Molecular detection and antimicrobial activity of Endophytic fungi isolated from a medical plant Rosmarinus officinalis

2020 ◽  
Vol 23 (13) ◽  
Author(s):  
Shimal Yonuis Abdulhadi ◽  
Ghazwan Qasim Hasan ◽  
Raghad Nawaf Gergees
Keyword(s):  
Antimicrobial Activity ◽  
Endophytic Fungi ◽  
Molecular Detection ◽  
Rosmarinus Officinalis ◽  
Medical Plant

scholarly journals Evaluation of antimicrobial, antioxidant and cytotoxic properties of bioactive compounds produced from endophytic fungi of Himalayan yew (Taxus wallichiana) in Nepal

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 379 ◽  
Author(s):  
Dhurva Prasad Gauchan ◽  
Pratistha Kandel ◽  
Astha Tuladhar ◽  
Ashesh Acharya ◽  
Upendra Kadel ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Endophytic Fungi ◽  
Diffusion Method ◽  
Dry Extract ◽  
Brine Shrimp Lethality Assay ◽  
Fungal Extracts ◽  
Taxus Wallichiana

Background: Endophytic fungi are largely underexplored in the discovery of natural bioactive products though being rich sources of novel compounds with promising pharmaceutical potential. In this study, Taxus wallichiana, which has huge medicinal value, was investigated for its endophytic diversity and capability to produce bioactive secondary metabolites by analyzing antioxidant, antimicrobial and cytotoxic properties. Methods: The endophytes were identified by ITS-PCR using genomic DNA samples. The secondary metabolites were extracted by solvent extraction method using ethyl acetate. The antioxidant activity was analyzed by Thin Layer Chromatography, Total Phenol Content (TPC), Total Flavonoid Content (TFC) and DPPH assay, and the antimicrobial activity was analyzed by agar-well diffusion method. Brine shrimp lethality assay was used to analyze the cytotoxicity of the fungal extracts. Results: Out of 16 different Taxus trees sampled from different locations of Dhorpatan, 13 distinctive endophytic fungi were isolated and grouped into 9 different genera: Bjerkandera, Trichoderma, Preussia, Botrytis, Arthrinium, Alternaria, Cladosporium, Sporormiella and Daldinia. The ethyl acetate extracts isolated from three endophytic fungi: Alternaria alternata, Cladosporium cladosporioides and Alternaria brassicae showed significant TPC values of 204±6.144, 312.3±2.147 and 152.7±4.958µg GAE/mg of dry extract, respectively, and TFC values of 177.9±2.911, 644.1±4.202 and 96.38±3.851µg RE/mg of dry extract, respectively. Furthermore, these three extracts showed a dose dependent radical scavenging activity with IC50 concentration of 22.85, 22.15 and 23.001 µg/ml, respectively. The extracts of C. cladosporioides and A. brassicae also showed promising antimicrobial activity against Escherichia coli, Staphylococcus aureus and Bacillus subtilis with a minimum inhibitory concentration of 250μg/ml for all bacteria. Both the samples showed cytotoxic property against shrimp nauplii with LC50 of 104.2 and 125.9µg/ml, respectively. Conclusions: The crude fungal extracts obtained from endophytes: A. alternata, C. cladosporioides and A. brassicae upon purification and further identification of the bioactive compounds can be a fascinating source for novel pharmaceutical agents.

scholarly journals Antimicrobial and larvicidal potential of endophytic fungi isolated from Tragia involucrata Linn.

2016 ◽  
Vol 6 (01) ◽  
pp. 1494 ◽  
Author(s):  
Ananda Danagoudar ◽  
Chandrashekhar G. Joshi* ◽  
M.T. Nivya ◽  
H.M. Manjunath ◽  
Jagadeesha Poyya ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Larvicidal Activity ◽  
Endophytic Fungi ◽  
Ethyl Acetate Extract ◽  
Penicillium Citrinum ◽  
Diffusion Method ◽  
Disc Diffusion Method ◽  
The People ◽  
Therapeutic Compounds

Endophytic fungi are one of the untapped resources of therapeutic compounds for various diseases. The present study focused on the antimicrobial as well as larvicidal activity of ethyl acetate extract of endophytic fungi isolated from Tragia involucrata. The ethyl acetate extract of Penicillium citrinum CGJ-C1 (GenBank No.KT780618), Penicillium citrinum CGJ-C2 (KP739821), Cladosporium sp. (KP739822), and Cryptendoxyla hypophloia CGJ-D2 (KT780619) was subjected to antimicrobial activity against a panel of microorganisms by disc diffusion method, larvicidal activity against Culex quinquefasciatus. All the extracts showed significant antimicrobial activity against the tested organisms ranging from 8±0.32 to 13±2.11. The extent of activity was comparable to the standard drugs. The larvicidal potential of the endophytes was superior to T.involucrata extract. The larvicidal activity was found to be dose and time dependent with LC50 value ranging from 4.25- 158.06ppm after 24hrs of treatment. This is the first report on the bioactivity of the endophytes isolated from T.involucrata. Further studies on the bio-guided isolation of lead compound will benefit the people suffering from microbe’s related diseases.

scholarly journals Endophytic Fungi from Sorghum bicolor (L.) Moench: Influence of Genotypes and Crop Systems and Evaluation of Antimicrobial Activity

2018 ◽  
Vol 8 (5) ◽  
Author(s):  
Ruth Terezinha Rodrigues ◽  
Márcia Marília de Souza Silva ◽  
Douglas Moreira de Oliveira ◽  
Josimar Bento Simplício ◽  
Cynthia Maria Carneiro Costa ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Sorghum Bicolor ◽  
Endophytic Fungi ◽  
Sorghum Bicolor L

scholarly journals Antimicrobial activity of green silver nanoparticles from endophytic fungi isolated from Calotropis procera (Ait) latex

Microbiology ◽  
2019 ◽  
Vol 165 (9) ◽  
pp. 967-975 ◽  
Author(s):  
Nadia Hussein Mohamed ◽  
Mady Ahmed Ismail ◽  
Wael Mostafa Abdel-Mageed ◽  
Ahmed Abdelfattah Mohamed Shoreit
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Endophytic Fungi ◽  
Calotropis Procera

scholarly journals Chemical Composition and in Vitro Antimicrobial Activity of the Volatile Oils from Gliomastix murorum and Pichia guilliermondii, Two Endophytic Fungi in Paris polyphylla var. yunnanensis

2009 ◽  
Vol 4 (11) ◽  
pp. 1934578X0900401 ◽  
Author(s):  
Jianglin Zhao ◽  
Tijiang Shan ◽  
Yongfu Huang ◽  
Xili Liu ◽  
Xiwu Gao ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Endophytic Fungi ◽  
Inhibitory Concentration ◽  
Octadecenoic Acid ◽  
Volatile Oil ◽  
Pichia Guilliermondii ◽  
Volatile Oils ◽  
Paris Polyphylla ◽  
Gliomastix Murorum

Volatile oils were obtained by hydrodistillation from Gliomastix murorum and Pichia guilliermondii, two endophytic fungi isolated from the traditional Chinese medicinal herb Paris polyphylla var. yunnanensis. The oils were analyzed for their chemical composition by gas chromatography-mass spectrometry (GC-MS). Palmitic acid (15.5%), (E)-9-octadecenoic acid (11.6%), 6-pentyl-5,6-dihydropyran-2-one (9.7%), and (7Z,10Z)-7,10- hexadecadienoic acid (8.3%) were the major compounds of the 40 identified components in G. murorum volatile oil. 1,1,3a,7-Tetramethyl-1a,2,3,3a,4,5,6,7b-octahydro-1H-cyclopropa[a]- naphthalene (25.9%), palmitic acid (15.5%), 1-methyl-2,4-di- (prop-1-en-2-yl)-1- vinylcyclohexane (7.9%), (E)-9-octadecenoic acid (7.3%), and (9E,12E)-ethyl-9,12-octadecadienoate (5.2%) were the major compounds of the 27 identified components in P. guilliermondii volatile oil. The in vitro antimicrobial activity of the volatile oils was also investigated to evaluate their efficacy against six bacteria and one phytopathogenic fungus. The minimum inhibitory concentration (MIC) values of the volatile oils against the test bacteria ranged from 0.20 mg/mL to 1.50 mg/mL. One of the most sensitive bacteria was Xanthomonas vesicatoria with an MIC of 0.20 mg/mL and 0.40 mg/mL for G. murorum and P. guilliermondii, respectively. The mean inhibitory concentration (IC50) of the volatile oils against spore germination of Magnaporthe oryzae was 0.84 mg/mL for G. murorum and 1.56 mg/mL for P. guilliermondii. These results indicated that the volatile oils from the endophytic fungi have strong antimicrobial activity and could be a potential source of antimicrobial ingredients.

scholarly journals Allium sativum, Rosmarinus officinalis, and Salvia officinalis Essential Oils: A Spiced Shield against Blowflies

Insects ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 143 ◽  
Author(s):  
Stefano Bedini ◽  
Salvatore Guarino ◽  
Maria Cristina Echeverria ◽  
Guido Flamini ◽  
Roberta Ascrizzi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Foodborne Pathogens ◽  
Allium Sativum ◽  
Insect Pests ◽  
Meat Products ◽  
Rosmarinus Officinalis ◽  
Salvia Officinalis ◽  
Meat Processing ◽  
Toxic Activity

Blowflies are known vectors of many foodborne pathogens and unintentional human ingestion of maggots by meat consumption may lead to intestinal myiasis. In fact, the control of insect pests is an important aspect of industrial and home-made food processing and blowflies (Diptera: Calliphoridae), which are among the most important pests involved in the damage of meat products. Most spices, largely used in food preparations and industry, contain essential oils that are toxic and repellent against insects and exert antimicrobial activity. In this study, we assessed the electro-antennographic responses, the oviposition deterrence, the toxicity, and the repellence of the essential oils (EOs) of Allium sativum L., Salvia officinalis L., and Rosmarinus officinalis L. against the blowfly Calliphora vomitoria L. We tested the EOs antibacterial and antifungal properties and the efficacy of an A. sativum EO-charged mist sprayed in the tunnel entryway of a meat processing room to form an olfactive barrier against the entrance of flies. The results showed that the EOs are perceived by female blowfly’ antennae and exert an evident repellent activity against them completely deterring the oviposition for up to 24 h starting from the concentration of 2.5 μL cm−2 EO. The EOs also exhibited toxic activity by both topical application (LD50 from 0.44 to 1.97 μL insect−1) and fumigation (LC50 from 1.76 to 31.52 μL L−1) against adults of C. vomitoria and were able to exert a clear antimicrobial activity toward pathogens. Lastly, the EO-charged mist was able to reduce by about 40% the presence of Calliphoridae in the meat processing room of a dry-ham factory.

scholarly journals Diversity and Antimicrobial Activity of Endophytic Fungi Isolated from Chloranthus japonicus Sieb in Qinling Mountains, China

2020 ◽  
Vol 21 (17) ◽  
pp. 5958
Author(s):  
Chao An ◽  
Saijian Ma ◽  
Xinwei Shi ◽  
Wenjiao Xue ◽  
Chen Liu ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Endophytic Fungi ◽  
Wiener Index ◽  
Opportunistic Pathogen ◽  
Qinling Mountains ◽  
Anticancer Properties ◽  
Abundant Genus ◽  
Antibacterial And Antifungal ◽  
Richness Index ◽  
Ethyl Acetate Extracts

The plant Chloranthus japonicus Sieb is known for its anticancer properties and mainly distributed in China, Japan, and Korea. In this study, we firstly investigated the diversity and antimicrobial activity of the culturable endophytic fungi from C. japonicus. A total of 332 fungal colonies were successfully isolated from 555 tissue segments of the medicinal plant C. japonicus collected from Qinling Mountains, China. One hundred and thirty representative morphotype strains were identified according to ITS rDNA sequence analyses and were grouped into three phyla (Ascomycota, Basidiomycota, Mucoromycota), five classes (Dothideomycetes, Sordariomycetes, Eurotiomycetes, Agaricomycetes, Mucoromycetes), and at least 30 genera. Colletotrichum (RA, 60.54%) was the most abundant genus, followed by Aspergillus (RA, 11.75%) and Diaporthe (RA, 9.34%). The Species Richness Index (S, 56) and the Shannon-Wiener Index (H′, 2.7076) indicated that C. japonicus harbored abundant fungal resources. Thirteen out of 130 endophytic fungal ethyl acetate extracts exhibited inhibitory activities against at least one pathogenic bacterium or fungus. Among of these, F8158, which was identified as Trichoderma cf. harzianum, exhibited good antagonistic capacities (the percent inhibition of mycelial growth ranged from 47.72~88.18) for different pathogens and has a potential application in biological control. In addition, it is noteworthy that the strain F8157 (Thanatephorus cucumeris, an opportunistic pathogen) showed antibacterial and antifungal activity, which is reported firstly in this study, and should be investigated further. Taken together, these results indicated that the endophytic fungi from C. japonicus may be of potential interest in screening bio-control agents and discovering of new bioactive compounds.

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