scholarly journals Morphologic Pattern of Diseases in Gastric Biopsies and Role of Helicobacter Pylori in Chronic Gastritis at a Nigerian Teaching Hospital

2021 ◽  
Vol 6 (2) ◽  
pp. 71-75
Author(s):  
  Usman Bello ◽  
Abubakar S Maiyaki
Keyword(s):  
Helicobacter Pylori ◽  
Teaching Hospital ◽  
Chronic Gastritis ◽  
Gastric Biopsies

scholarly journals Detection of Helicobacter pylori Through Histochemistry & Immunofluorescent Staining in Biopsies of Patients with Chronic Gastritis

2011 ◽  
Vol 34 (4) ◽  
pp. 4-9
Author(s):  
Asia Jahanzeb ◽  
Abdul Hannan Nagi Nagi ◽  
Ayesha Suleman
Keyword(s):  
Helicobacter Pylori ◽  
Chronic Gastritis ◽  
Histopathological Examination ◽  
Endoscopic Examination ◽  
Immunofluorescent Staining ◽  
Rapid Urease Test ◽  
Specific Method ◽  
Total N ◽  
Urease Test ◽  
Gastric Biopsies

Introduction: Helicobacter pylori (H.pylori) is a helix shaped gram negative rod which is usually associated with chronic gastritis and also a major cause of other astroduodenal diseases as well. Aims & Objectives: The present study used histochemical and immunofluorescent stains on formalin fixed paraffin embedded human gastric biopsies for detection of H.pylori. Comparison was also done to evaluate best staining method. Place and duration of study: This study was accomplished in about one year. Sampling of gastric biopsies and rapid urease test were executed at the endoscopy suite of Lahore General Hospital whereas the histopathological examination and immunofluorescent staining were done in University of Health Sciences, Lahore. Material & Methods: Thirty patients (n=30cases) were included in the study following inclusion criteria. Diagnostic upper GI endoscopy was carried out in all cases. Five gastric biopsies were taken from each patient/case (total N=150 Biopsies) according to the Updated Sydney System. Rapid urease test was performed at the site of endoscopy. Biopsies fixed in 10% formalin were brought to the concerned department where they were assigned a specific laboratory number then processed and stained. Results: Endoscopic examination revealed chronic gastritis and rapid urease tests were positive. All cases (n=30) were positive for H.pylori on histopathology. The calculated sensitivity and specificity of H&E, Giemsa, Modified McMullen’s stain and Immunofluorescent method in present study were 71% and 100%,83% and 100%, 82% and 100%, 90% and 100% respectively. Conclusion: Special stains makes H.pylori identification easier in tissue sections. However, immunofluorescent test is the most sensitive and specific method as compared to histochemical stains.

The role of Helicobacter pylori in the chronic gastritis and stomach cancer formation: international guidelines review and own research results

Therapy ◽  
2020 ◽  
Vol 4_2020 ◽  
pp. 102-109
Author(s):  
Luzina E.V. Luzina ◽  
Chartorizhskaya N.N. Chartorizhskaya ◽  
Melnikov V.V. Melnikov ◽  
Lareva N.V. Lareva ◽  
◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Stomach Cancer ◽  
Chronic Gastritis ◽  
International Guidelines ◽  
Research Results

scholarly journals Comparative analysis of two-dimensional electrophoresis maps (2-DE) of Helicobacter pylori from Brazilian patients with chronic gastritis and duodenal ulcer: a preliminary report

2006 ◽  
Vol 48 (3) ◽  
pp. 175-177 ◽  
Author(s):  
Diego R.B. Pereira ◽  
Daniel Martins ◽  
Flavia V. Winck ◽  
Marcus B. Smolka ◽  
Nancy F. Nishimura ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Comparative Analysis ◽  
Chronic Gastritis ◽  
Protein Identification ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Duodenal Ulcers ◽  
Pathogenic Factors ◽  
Gastric Biopsies ◽  
H Pylori

Helicobacter pylori is a bacterium recognized as the major cause of peptic ulcer and chronic gastritis. Recently, a proteome-based approach was developed to investigate pathogenic factors related to H. pylori. In this preliminary study, H. pylori strains were isolated from gastric biopsies of patients with chronic gastritis and duodenal ulcers. A partial proteomic analysis of H. pylori strains was performed by bacterial lyses and proteins were separated by two-dimensional gel electrophoresis (2-DE). A comparative analysis was performed to verify a differential protein expression between these two 2-DE maps. These data should be useful to clarify the role of different proteins related to bacterial pathogenesis. This study will be completed using a larger number of samples and protein identification of H. pylori by MALDI-TOF mass spectrometry.

scholarly journals Efficacy of immunohistochemical staining in detecting Helicobacter pylori in Saudi patients with minimal and atypical infection

2021 ◽  
Vol 65 (3) ◽  
Author(s):  
Mohammed Akeel ◽  
Ahmed Elhafey ◽  
Atef Shehata ◽  
Erwa Elmakki ◽  
Thanaa Aboshouk ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Chronic Gastritis ◽  
Direct Detection ◽  
Giemsa Staining ◽  
Pcr Analysis ◽  
Qrt Pcr ◽  
Urease Test ◽  
Gastric Biopsies ◽  
Polymerase Chain ◽  
H Pylori

Gastric Helicobacter pylori infection is diagnosed based on histopathological evaluation of gastric mucosal biopsies, urease test, urea breath test, H. pylori culturing, or direct detection using polymerase chain reaction (PCR). This study aimed to evaluate the efficacy of immunohistochemical (IHC) staining in detecting H. pylori in gastric biopsies from patients with chronic gastritis and minimal or atypical infection. Gastric biopsies from 50 patients with chronic gastritis were subjected to routine haematoxylin and eosin (H&E), modified Giemsa, and IHC staining. The results of staining were compared with those of quantitative real-time PCR (qRT-PCR). The qRT-PCR analysis identified 32 (64%) H. pylori-positive cases, whereas IHC, H&E, and modified Giemsa staining identified 29 (58%), 27 (54%), and 21 (42%) positive cases. The sensitivity of IHC staining (87.50%) was higher than that of H&E (59.38%) and modified Giemsa (43.75%) staining. The specificity of H&E, modified Giemsa, and IHC staining was 55.56%, 61.11%, and 94.44%, respectively. IHC staining exhibited the highest diagnostic accuracy (90%), followed by H&E (58%) and modified Giemsa (50%) staining. Active gastritis, intestinal metaplasia, and lymphoid follicles were detected in 32 (64%), 4 (8%), and 22 (44%) cases, respectively, and all of these cases were H. pylori positive. In contrast to routine H&E and modified Giemsa staining, IHC allows for the accurate H. pylori detection in cases with minimal or atypical infection. Moreover, IHC can be an alternative diagnostic method to qRT-PCR for detection of H. pylori in such cases.

scholarly journals Problematic issues of chronic gastritis studies

2019 ◽  
Vol 44 (3) ◽  
pp. 54-61
Author(s):  
Y. S. Tsimmerman ◽  
Yu. A. Zakharova
Keyword(s):  
Helicobacter Pylori ◽  
Chronic Gastritis ◽  
Inflammatory Process ◽  
History Of ◽  
Histological Characteristics ◽  
Functional Features ◽  
Bacterial Associations ◽  
The 19Th Century ◽  
Gastric Microflora

The article defines chronic gastritis as a polyetiological and polypathogenetic stomach disease with a chronic, slowly progressing course, which is based on a specific inflammatory process with lymphoplasmocytic infiltration of its mucosa and neutrophilic component indicating its activity, and with development of disregenerative, dystrophic changes, leading to its secretory insufficiency, manifested hypo- and achlorhydria and gastric achilia. The history of studying chronic gastritis from the beginning of the 19th century till present days is briefly described. It is proposed to distinguish between causal (Helicobacter pylori, etc.) and predisposing (alcohol, smoking, coarse food, etc.) factors in the development of chronic gastritis. The analysis of various classifications of gastritis is carried out: based on etiology, pathogenesis, functional features, clinic, endoscopic and histological characteristics. The Sydney, Houston classifications, the OLGA system are described. Particular attention is paid to diagnosis, biopsy technique of the gastric mucosa, ratio of diagnoses of chronic gastritis and functional dyspepsia, as well as the role of gastric microflora in development of gastritis. It is revealed that gastric microflora in chronic gastritis is represented by numerous types of bacteria (more often in the form of bacterial associations), moreover, Helicobacter pylori is not the dominant microorganism colonizing the stomach, and the mucosal microflora found in the stomach has adhesiveness, invasiveness and pathogenic properties, including its urease activity.

scholarly journals Kyoto consensus - the new etiological classification of chronic gastritis and its discussion

2017 ◽  
Vol 95 (2) ◽  
pp. 181-188
Author(s):  
Yakov S. Tsimmerman ◽  
Yu. A. Zakharova
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Mucosa ◽  
Chronic Gastritis ◽  
Evidence Based Medicine ◽  
Urease Activity ◽  
Evidence Based ◽  
Based Medicine ◽  
Gastric Microflora

The main provisions and headings of the new etiological classification of chronic gastritis are discussed in the context of recent data on the true role of Helicobacter pylori infection in the development of this pathology. The methods and results of the authors ’ investigations into gastric microflora are presented along with information about the frequency of detection of its different forms, concentration of microbial forms in gastric mucosa, their pathogenic properties including urease activity, and possible contribution to chronic gastritis etiology. The possibility of alcoholic and chemical chronic gastritis is discussed along with the role of these conditions in the development of stomach cancer. Special emphasis is laid on the disagreement between the adopted consensuses and principles of evidence-based medicine.

scholarly journals Functional dyspepsia and chronic gastritis

2018 ◽  
Vol 9 (1) ◽  
pp. 77-83 ◽  
Author(s):  
Yury P. Uspenskiy ◽  
Natalia V. Baryshnikova
Keyword(s):  
Helicobacter Pylori ◽  
Digestive Tract ◽  
Functional Dyspepsia ◽  
Chronic Gastritis ◽  
Eradication Therapy ◽  
Point Of View ◽  
Etiological Role ◽  
Working Age ◽  
Social Importance

Diseases of upper parts of digestive tract are extremely widespread pathology and have not only the medical, but also social importance since often occur at young working-age people. It is actual to separate the items: functional dyspepsia (FD) and a dyspepsia associated with Helicobacter pylori because it is important for optimization of patient’s treatment. The review of modern foreign and Russian references about treatment of dyspeptic patients depending on existence or absence of an infection of Helicobacter pylori is presented in article (recommendation of V Maastricht consensus, Kyoto consensus, Russian gastroenterological association, Science society of Russian gastroenterologists). At diagnosis of a functional dyspepsia, it is recommended to be guided by the Roman criteria of the IV revision. In with document not only importance of an exception of organic pathology of digestive tract as the reasons of a secondary dyspepsia, but also need of inspection for the purpose of verification of Helicobacter pylori for an exception of an etiological role of a microorganism in development of a dyspepsia is emphasized. Allocation of two clinical options of a functional dyspepsia is also proved from the point of view of approaches to therapy of this disease. Algorithms of treatment of patients with various options of a dyspepsia are presented in article. These algorithms include different schemes of eradication therapy in case of dyspepsia associated with Helicobacter pylori and prokinetics/acid suppressors in case of functional dyspepsia.

A COMPARATIVE STUDY OF HEMATOXYLIN & EOSIN, SPECIAL STAINS AND IMMUNOHISTOCHEMISTRY FOR DETECTION OF HELICOBACTER PYLORI IN CASES OF GASTRITIS

2021 ◽  
pp. 21-24
Author(s):  
Umasankari. L. Ramasamy ◽  
Manjula Rajendran
Keyword(s):  
Helicobacter Pylori ◽  
Peptic Ulcer ◽  
Toluidine Blue ◽  
Cost Effective ◽  
Biopsy Specimens ◽  
Gastric Biopsies ◽  
Hematoxylin And Eosin ◽  
Hematoxylin Eosin ◽  
Upper Gastrointestinal

Background: The role of Helicobacter Pylori in the pathogenesis of Gastritis-Peptic ulcer syndrome and its association with the development of upper gastrointestinal tract malignancy warrant efcient method for the identication of the bacteria in biopsy specimens. Four methods - Hematoxylin and Eosin, Giemsa, Toluidine Blue and Immunohistochemistry were compared to detect the presence of Helicobacter pylori. The prevalence of H.Pylori in gastric biopsies was also evaluated. Aim Of The Study: The aim is to evaluate the prevalence of H.Pylori in cases of gastritis and compare the efcacy of Hematoxylin and Eosin, Giemsa , Toluidine blue and Immunohistochemistry for the detection of H.pylori in cases of gastritis. Materials And Methods: This Retrospective study was done in the Department of Pathology. A total of 40 cases of gastritis were randomly selected for this study and all the four methods were applied. Results: Helicobacter pylori infection showed an overall prevalence rate of about 75%. When compared with Immunohistochemistry, Sensitivity and specicity of Hematoxylin and Eosin was 66.67% and 100% respectively. Giemsa showed sensitivity of about 86.67% and specicity of about 100%. Toluidine blue showed sensitivity of about 80% and specicity of about 100%. Conclusion: Hence in the present study Giemsa was more reliable and cost effective stain when compared with Hematoxylin & Eosin, Toluidine blue and immunohistochemistry. However, Immunohistochemistry carries the highest level of sensitivity in the detection of Helicobacter Pylori especially when the density of organism is low and in clinically suspected cases of Helicobacter Pylori with negative Giemsa staining

Sign in / Sign up

Export Citation Format

Share Document