THE INFLUENCE OF POLYHEXAMETHYLENE GUANIDINE SALTS ON THE MICROBIOLOGICAL PARAMETERS OF WATER

The article represent the use of disinfectant which was made based on salts of polyhexamethylene guanidine hydrochloride (PGMG-hydrochloride) and polyhexamethylene guanidine phosphate (PGMG-phosphate) for water disinfection. Water plays one of the most important roles in almost all life processes. Water is a part of the blood, participates in blood circulatory system, delivers nutrients and oxygen to the organs, participates in oxidation reaction, hydrolysis and other reactions of intercellular metabolism, forms an environment to maintain a healthy microflora of the digestive tract, which provides effective breakdown of nutrients by the optimal amount of enzymes. Good drinking water quality must to be epidemically safe and free of pathogenic microbes, viruses and other biological inclusions. Also must be harmless in chemical composition, have good organoleptic properties ‒ be transparent, colorless, have no taste or odor. Water quality is assessed by total microbial contamination and the amount of bacteria of the Escherichia coli group in 1 cm3 of water, and also by thermostable Escherichia coli (fecal coliforms) in 100 cm3 of water, pathogenic microorganisms and the amount of coli-phages. In result of the conducted researches it was found that the use of disinfectant in 0,2 % concentration did not reduce the total microbial pollution of water. In particular, the efficiency of 1, 2 and 3-hour of exposure was 57, 62 and 68 %, respectively. After application of disinfectant in 0,3 % concentration the efficiency of its application was for 1 h – 69 %, 2 h – 82 %, 3 h – 100 %. It was found that the use of a disinfectant, which was made based on PGMG salts in 0,4 % concentration contributed to reduce the total microbial contamination of water. The efficiency by the 1, 2 and 3 hour of exposure was, respectively, 73, 86 and 100 %. Therefore, the use of a disinfectant, containing in its composition PGMG salts, in 0,3% concentration at 3 hours of exposure was more optimal, which contributed to reduce the total microbial contamination of water.

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Análise Microbiológica da Qualidade das Águas do Rio Cuiabá Perímetro Urbano, a Partir de 4 Pontos de Amostragem

UNICIÊNCIAS ◽

10.17921/1415-5141.2017v21n2p56-59 ◽

2017 ◽

Vol 21 (2) ◽

pp. 56

Author(s):

Waléria Finicia de Oliveira ◽

Osvaldo Borges Pinto

Keyword(s):

Escherichia Coli ◽

Water Quality ◽

Biological Indicators ◽

Domestic Sewage ◽

Human Consumption ◽

River Waters ◽

Sampling Points ◽

Microbiological Parameters ◽

Quality Water ◽

Consequent Increase

A ocupação eminentemente urbana dos municípios resultou em um incremento da demanda nos diversos usos das águas do rio Cuiabá e consequente aumento das cargas orgânicas, de nutrientes e de coliformes gerados pelos esgotos domésticos. As microbacias hidrográficas, com vegetação natural remanescente, são áreas muito importantes para manter o abastecimento de água de boa qualidade. O objetivo deste trabalho foi realizar análises microbiológicas da qualidade da água do rio Cuiabá, especificamente no perímetro urbano, a partir de quatro pontos de amostragem, usando os parâmetros microbiológicos, a fim de quantificar Coliformes Totais e Escherichia coli. Foram feitas quatro coletas em pontos aleatórios, uma em 03/11/2014 e as outras três em 22/10/2015. Os resultados obtidos, nas análises feitas com as amostras, apontou como impróprio para o consumo humano, conforme a Resolução nº 357/05 do CONAMA.Palavras-chaves: Qualidade da Água. Bioindicadores, Escherichia coli.AbstratThe eminently urban occupation of the municipalities resulted in an increase in the demand for the different uses of the Cuiabá river waters and consequent increase in the organic loads, nutrients and coliforms generated by domestic sewage. The waters and consequent increase in the organic loads, nutrients and coliforms generated by domestic sewage. Thehydrographic basins with remaining natural vegetation arevery important areas to maintain the supply of good quality water. The objective of this work was to perform microbiological analyzes of the water quality, specifically at the urban perimeter, from 04 Sampling Points, using the microbiological parameters, in order to quantify Total Coliformes and Escherichia coli. Four collections were performed, one for each point, the first on November 3rd, 2014, another on October 22nd, 2015. The results obtained from the analyses performed with the samples, pointed as inadequate for human consumption, according to Resolution number 357/05 of CONAMA.Keywords: Water Quality. Biological Indicators. Escherichia coli

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Relationship between water quality parameters and the survival of indicator microorganisms – Escherichia coli – in a stormwater wetland

Water Science & Technology ◽

10.2166/wst.2013.386 ◽

2013 ◽

Vol 68 (7) ◽

pp. 1650-1656 ◽

Cited By ~ 4

Author(s):

Jing Cheng ◽

Siping Niu ◽

Youngchul Kim

Keyword(s):

Escherichia Coli ◽

Water Quality ◽

Pearson Correlation ◽

Dilution Effect ◽

Quality Parameters ◽

Water Quality Parameters ◽

Indicator Microorganisms ◽

E Coli ◽

Significant Seasonal Variation ◽

Almost All

Indicator microorganisms – Escherichia coli – in a stormwater wetland controlling non-point source pollution in the Keum River basin was studied and the relationship between water quality parameters and the survival of E. coli was investigated by Pearson correlation analysis based on 16 sets of data collected on dry days. A significant seasonal variation of the density of E. coli was observed in the influent and effluent. A dominant decay of E. coli was found in almost all the wetland components. In the settling pond, the density was observed to vary negatively with pH and the removal of total suspended solids, and the decay was promoted by solar radiation. In the aeration pond, the survival of E. coli was encouraged negatively by the dilution effect, unfavorable increase of dissolved oxygen (DO) and sedimentation with coarse particles. In the marsh wetland, the reduction of E. coli was positively influenced by UV radiation, obvious increase of pH, DO and sedimentation with algae and particles. In the polishing pond, the introduction of E. coli with avian feces and growth of the bacteria were more prevalent than decay. Although this introduction or growth was almost negligible, further study will be conducted in the future.

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Inequalities in microbial contamination of drinking water supplies in urban areas: the case of Lilongwe, Malawi

Journal of Water and Health ◽

10.2166/wh.2016.258 ◽

2016 ◽

Vol 14 (5) ◽

pp. 851-863 ◽

Cited By ~ 20

Author(s):

Akosua Sarpong Boakye-Ansah ◽

Giuliana Ferrero ◽

Maria Rusca ◽

Pieter van der Zaag

Keyword(s):

Escherichia Coli ◽

Water Quality ◽

Drinking Water ◽

Water Supply ◽

Low Income ◽

Urban Areas ◽

Microbial Contamination ◽

Water Storage ◽

Faecal Contamination ◽

Household Level

Over past decades strategies for improving access to drinking water in cities of the Global South have mainly focused on increasing coverage, while water quality has often been overlooked. This paper focuses on drinking water quality in the centralized water supply network of Lilongwe, the capital of Malawi. It shows how microbial contamination of drinking water is unequally distributed to consumers in low-income (unplanned areas) and higher-income neighbourhoods (planned areas). Microbial contamination and residual disinfectant concentration were measured in 170 water samples collected from in-house taps in high-income areas and from kiosks and water storage facilities in low-income areas between November 2014 and January 2015. Faecal contamination (Escherichia coli) was detected in 10% of the 40 samples collected from planned areas, in 59% of the 64 samples collected from kiosks in the unplanned areas and in 75% of the 32 samples of water stored at household level. Differences in water quality in planned and unplanned areas were found to be statistically significant at p < 0.05. Finally, the paper shows how the inequalities in microbial contamination of drinking water are produced by decisions both on the development of the water supply infrastructure and on how this is operated and maintained.

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Disinfection of Ready-to-Eat Lettuce Using Polyhexamethylene Guanidine Hydrochloride

Microorganisms ◽

10.3390/microorganisms8020272 ◽

2020 ◽

Vol 8 (2) ◽

pp. 272 ◽

Cited By ~ 1

Author(s):

Jiayi Wang ◽

Yougui Yu ◽

Yuemei Dong

Keyword(s):

Escherichia Coli ◽

Acetic Acid ◽

Guanidine Hydrochloride ◽

Agricultural Products ◽

Quality Analysis ◽

Escherichia Coli O157 ◽

Quality Loss ◽

Antibacterial Effects ◽

Polyhexamethylene Guanidine

As a novel and safe sanitizer, polyhexamethylene guanidine hydrochloride (PHMG) has been used to inhibit the spoilage of agricultural products caused by fungi. However, little is known about its antibacterial effects on vegetables. In this study, we evaluated the disinfection efficacy of PHMG on ready-to-eat lettuce. PHMG (150–200 mg/L) treatment for 5 min was optimal for lettuce disinfection. Compared to several household sanitizers (vinegar: 1% acetic acid; kettle descaler: 1% citric acid; “84” disinfectant: 200 mg/L sodium hypochlorite), PHMG showed the greatest reductions in Escherichia coli O157:H7, Listeria monocytogenes, aerobic mesophilic counts, aerobic psychrotrophic counts and molds and yeasts. Quality analysis of color (as determined by L*, a* and b*) and determination of electrolyte leakage indicated that PHMG did not cause any additional quality loss as compared to other household sanitizers. These results provide a reference for the application of PHMG as a vegetable sanitizer at the ready-to-eat stage.

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QUALIDADE DA ÁGUA EM ÁREAS RURAIS: Análise bacteriológica e físico-química das águas dos tanques de pedra das comunidades KM 21 (Campina Grande) e Pedra Redonda (Pocinhos) (Water quality in rural areas: bacteriological and physical-chemical analysis...)

Revista Brasileira de Geografia Física ◽

10.26848/rbgf.v9.6.p1737-1753 ◽

2016 ◽

Vol 9 (6) ◽

pp. 1737

Author(s):

Laíse Do Nascimento Cabral ◽

Sérgio Murilo Santos de Araújo

Keyword(s):

Escherichia Coli ◽

Water Quality ◽

Rural Communities ◽

Water Resources ◽

Chemical Analysis ◽

Rural Areas ◽

Human Consumption ◽

Physical Chemical ◽

Physico Chemical ◽

Microbiological Parameters

Nesse trabalho, foi analisada a qualidade da água em níveis bacteriológicos para consumo humano e demais usos em reservatórios denominados Tanques de Pedra nas comunidades rurais do Semiárido Paraibano: KM 21, Campina Grande (Agreste paraibano)-PB, e Pedra Redonda (Curimataú paraibano), Pocinhos-PB. Como critério de escolha das comunidades, considerou-se a importância dos reservatórios para a população, haja vista serem o principal reservatório para captação de água da chuva e armazenamento. A qualidade da água foi analisada a partir de sete coletas com a determinação dos parâmetros físico-químicos e microbiológicos. A metodologia aplicada foi o método enzimático de substrato definido Colilert. Para as análises físico-químicas, utilizou-se 24 parâmetros identificados pelo Valor Máximo Permissível (VMP). Os resultados, para parâmetros microbiológicos e físico-químicos, estão fundamentados na Portaria 2914/11 do Ministério da Saúde. Utilizou-se registro fotográfico para obtenção de imagens dos reservatórios. Os resultados mostraram que a qualidade da água das comunidades encontrava-se comprometida, tendo em vista que se encontrava infectada nos níveis bacteriológicos (Escherichia coli e Coliformes totais) e físico-químicos para cor e turbidez, sendo estes parâmetros utilizados para consumo humano. Assim, concluiu-se que, nos casos da utilização do recurso hídrico para usos múltiplos da água, pode haver utilização, porém com algumas restrições. Por fim, é válido destacar que os tanques de pedra são o maior provedor de água para as famílias de ambas as comunidades, por este motivo a análise da qualidade da água deu-se para este tipo de reservatório dado a sua importância aquífera e social. A B S T R A C T In this study, the water quality was analyzed for bacteriological levels for human consumption and other uses in so-called reservoirs Stone Tanks in rural communities Paraibano Semi-Arid: KM 21, Campina Grande (Agreste Paraibano)-PB, and Pedra Redonda (Curimataú Paraibano), Pocinhos-PB. As a criterion of choice of the communities, it considered the importance of reservoirs for the population, given as the primary reservoir for capturing rainwater and storage. The water quality was analyzed from seven collections with the determination of physical, chemical and microbiological parameters. The methodology applied was the enzymatic method of substrate defined Colilert. For the physico-chemical analysis, we used 24 parameters identified by the Maximum Permissible Value (MPV). The results for microbiological and physico-chemical parameters are based on the Ministry of Health Ordinance 2914/11. We used photographic record to obtain images of the reservoirs. The results showed that the water quality of communities found compromised, considering that was infected in the bacteriological level (Escherichia coli and Coliforms totals) and physico-chemical for color and turbidity, these parameters being used for human consumption. Thus, it was concluded that in cases where the use of water resources for multiple uses water can be used, but with some restrictions. Finally, it is worth noting that the stone tanks are the largest water provider for the families of both communities, for this reason the analysis of water quality was given for this type of reservoir given its importance aquifer and social. Keywords: Water resources; Sustainability; water management; Agreste of Paraíba; Brazilian semiarid.

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Monitoring of urban and rural basins: water quality of Mourão basin

Brazilian Journal of Biology ◽

10.1590/1519-6984.01213suppl ◽

2015 ◽

Vol 75 (4 suppl 2) ◽

pp. 158-164 ◽

Cited By ~ 3

Author(s):

F. H. Passig ◽

S. B. Lima ◽

K. Q. Carvalho ◽

M. C. R. Halmeman ◽

P. C. Souza ◽

...

Keyword(s):

Water Quality ◽

River Basin ◽

Rural Areas ◽

Oxygen Demand ◽

Fecal Coliforms ◽

Dry Period ◽

Volatile Solids ◽

Microbiological Parameters ◽

Nitrite Nitrate

The Mourão River basin is located on the central western region of the Paraná State – Brazil, between coordinates 23º 44’ - 24º 25 South latitude and 52º 12’ - 52º 30’ West longitude, between 270 and 820 m above sea level, and 1,648.21 km2 drainage area. Water quality was evaluated by monitoring physical, chemical and microbiological parameters. Monthly samplings were performed for a year at five sites in the basin for analysis of: pH, temperature, dissolved oxygen, biochemical oxygen demand, total nitrogen, ammoniacal nitrogen, nitrite, nitrate, total phosphorus, turbidity, total solids, volatile solids and fecal coliforms. The results of the evaluated parameters showed higher values than the limits set by CONAMA Resolution 357 from 2005 for Class 2 in some samples. The Water Quality Index (WQI) indicated that 72% of samples had average quality and 28% had good quality for the Mourão River basin. Higher values of WQI were observed after rainfall period with median of 75 compared to the dry period with median of 62. The source of the Mourão River is contaminated with fecal coliforms, evidencing the real need to treat sewage in rural areas.

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Assay of Potability with Bottled Mineral Water from Trademarks

Earth & Environmental Science Research & Reviews ◽

10.33140/eesrr.02.04.02 ◽

2019 ◽

Vol 2 (4) ◽

Keyword(s):

Escherichia Coli ◽

Water Quality ◽

Physicochemical Parameters ◽

Mineral Water ◽

Nitrate Nitrogen ◽

Human Consumption ◽

Microbiological Analysis ◽

Microbiological Parameters ◽

Confirmatory Tests ◽

Bottled Mineral Water

The purpose of this test was to evaluate the physicochemical (ammoniacal nitrate, nitrogen nitrate, nitrogen nitrite, pH, orthophosphate and turbidity) and microbiological parameters (Escherichia coli and total coliforms) of mineral water samples bottled trademarks and compare the results obtained with Brasilian Ministery of Healty Ordinance 2.914/11. For this purpose, it was used trademarks mineral water bottled sold in pet 300 and 500 ml bottles. For microbiological analysis it was used Colipaper (Alfakit®), and all of them the analysis of physicochemical parameters was used Ecokit II (Alfakit®). From the five mineral water bottled samples, all were within the physicochemical parameters determined by Ordinance 2.914/11. In the microbiological findings, only one sample presented Escherichia coli. Therefore, all the analyzed brands meet the required physicochemical parameters of water quality for human consumption, except brand A, which did not meet the microbiological parameters. The results demonstrates the need for confirmatory tests performed by laboratories qualified to attest the result obtained, as well as the need to implement more effective monitoring by the responsible agency for monitoring the water quality for human consumption.

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Water Quality Sampling in Billy Barquedier National Park and Surrounding Areas, Belize, C.A.

Journal of Student Research ◽

10.47611/jsr.v10i3.1326 ◽

2021 ◽

Vol 10 (3) ◽

Author(s):

Taylor Boatwright ◽

Briana Monarca ◽

Minerva Brauss ◽

K. Daniel Brauss

Keyword(s):

Escherichia Coli ◽

Water Quality ◽

Water Samples ◽

National Park ◽

Water Source ◽

Fecal Coliforms ◽

Human Need ◽

Surrounding Areas ◽

Drinking Quality ◽

Physical Components

Looking at the physical, microbial, and chemical makeup of water samples gives a good indication of the drinking quality, which is important as it’s a vital human need. Testing water from Billy Barquedier National Park and the Valley community in the Stann Creek District, Belize, CA. is important to tell whether contamination is taking place from the water source or in the pipe systems. Testing involved taking samples as well as testing in the field with the Pro DSS water quality meter. Lab testing used a colorimeter and autoclave to test the microbial and chemical makeup. It’s important to look to at the connection between the chemical and physical components compared to the microbial components to see connections between harmful bacteria such as Escherichia coli and fecal coliforms.

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Bacteriological assessment of bottled drinking water in 5 provinces of central highland, VietNam

Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam ◽

10.47866/2615-9252/vjfc.75 ◽

2019 ◽

Vol 2 (3) ◽

pp. 86-89

Author(s):

Thuan Nguyen Vu ◽

Doanh Pham Van ◽

Thu Hien Nguyen Thi ◽

◽

...

Keyword(s):

Escherichia Coli ◽

Water Quality ◽

Pseudomonas Aeruginosa ◽

Drinking Water ◽

Microbial Contamination ◽

Total Coliform ◽

Bottled Drinking Water ◽

Bacteriological Quality ◽

Drinking Water Standard ◽

Central Highland

The assessment of the bacteriological quality among 390 samples of bottled drinking water was studied. The samples were tested for Escherichia coli, Clostridia, total Coliform, Streptococci feacal and Pseudomonas aeruginosa. The rates of microbial contamination in bottled drinking water were of 56.7% (2017) and 50.7% (2018). The samples failed to meet QCVN drinking water standard of acceptable limits of bacterial presence including Pseudomonas aeruginosa (41.5%), total Coliform (11.8%), Clostridia (4.6%); Escherichia coli (4.9%) and Streptococcifeacal (1.3%). It is necessary to strengthen the inspection, supervision, propagation, and education that give warnings, ensure water quality and raise the awareness of producers, traders and consumers.

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