scholarly journals Tingkat Pengetahuan Mahasiswa Fakultas Kedokteran Universitas Yarsi Mengenai Penggunaan Human Diploid Cell Dalam Proses Produksi Vaksin Polio

2021 ◽  
Vol 1 (12) ◽  
Author(s):  
Wahyu Fitrah Darwanto Nugroho ◽  
Indra Kusuma ◽  
Siti Nur Riani
Keyword(s):  
Hepatitis A ◽  
Oral Polio Vaccine ◽  
Simple Random Sampling ◽  
Diploid Cell ◽  
Human Diploid Cell ◽  
Cross Sectional ◽  
Inactivated Polio Vaccine ◽  
Polio Vaccine ◽  
Human Diploid Cells ◽  
Diploid Cells

Latar Belakang : Vaksin merupakan suspensi mikroorganisme yang dilemahkan atau dimatikan, atau protein antikgenik dari berbagai organisme tadi yang diberikan untuk mencegah, meringankan, atau mengobati penyakit-penyakit menular. Vaksin pertama kali tercatat pada tahun 1769, yang dipublikasikan oleh Edward Jenner, yaitu specimen yang berasal dari lesi lengan seseorang yang terinfeksi Cowpox. Human Diploid Cells (HDC) merupakan salah satu sel yang digunakan untuk mengkultur virus yang akan dijadikan vaksin. HDC yang berasal dari aborsi manusia ini banyak digunakan untuk mengkultur virus Polio IPV dan OPV, Rabies, Rubella, Measles, Varicella-Zooster, dan Hepatitis A. Tujuan : Vaksin polio merupakan vaksin yang diwajibkan pada anak yang dijadwalkan dari Ikatan Dokter Anak Indonesia (IDAI) yang dibagi menjadi dua jenis, IPV (Inactivated Polio Vaccine) dan OPV (Oral Polio Vaccine). Metode : Jenis Penelitian yang digunakan adalah deskriptif dengan pendekatan cross sectional menggunakan kuesioner. Populasi yang digunakan adalah mahasisa Fakultas Kedokteran Universitas YARSI tahun pertama dan tahun ketiga yang memenuhi syarat. Cara pemilihan sampel dengan simple random sampling. Hasil : Penelitian yang dilaksanakan selama 3 hari dengan menggunakan kuesioner, dari 100 responden didapatkan persentase jumlah kuesioner Pengetahuan mengenai Human Diploid Cell berdasarkan Tingkat Pendidikan didapatkan pengetahuan baik sebanyak 5% pada tahun ketiga dan 7% pada tahun pertama. Pengetahuan cukup sebanyak 23% pada tingkat ketiga dan 28% pada tahun pertama. Pengetahuan kurang sebanyak 9% pada tingkat ketiga dan 28% pada tahun pertama. Persentase jumlah kuesioner Pengetahuan mengenai Polio berdasarkan Tingkat Pendidikan didapatkan pengetahuan baik sebanyak 15% pada tahun ketiga dan 19% pada tahun pertama. Pengetahuan cukup sebanyak 18% pada tingkat ketiga dan 31% pada tahun pertama. Pengetahuan kurang sebanyak 4% pada tingkat ketiga dan 13% pada tahun pertama. Kesimpulan : Tidak terdapat hubungan antara tingkat pendidikan dengan pengetahuan mengenai Human Diploid Cell dalam vaksin Polio. Dalam pandangan Islam, penggunaan vaksin Polio hukumnya mubah karena prinsip Dharuriyat bertujuan untuk mempertahankan nyawa atau Hifdz an-nafs anak dari ancaman penyakit.

scholarly journals Factors associated with the utilization of inactivated polio vaccine among children aged 12 to 23 months in Kalungu District, Uganda

2020 ◽  
Vol 35 (Supplement_1) ◽  
pp. i30-i37
Author(s):  
Mirembe Rachel Faith ◽  
Babirye Juliet ◽  
Nathan Tumuhamye ◽  
Tumwebaze Mathias ◽  
Emma Sacks
Keyword(s):  
Health Care Providers ◽  
Health Workers ◽  
Oral Polio Vaccine ◽  
Systematic Sampling ◽  
Care Providers ◽  
Cross Sectional ◽  
Inactivated Polio Vaccine ◽  
Factors Associated ◽  
Polio Vaccine ◽  
Eradication Strategy

Abstract Uganda officially introduced the inactivated polio vaccine (IPV) in May 2016 as part of the polio eradication strategy and integrated it into its routine immunization programme in addition to the oral polio vaccine. The current coverage stands at 60% as of July 2017. We therefore aimed to determine factors associated with the uptake of IPV among children in Kalungu District so as to inform the implementation of the vaccine policy. A community-based cross-sectional study was conducted among caregivers of 406 eligible children aged 12–23 months through multi-stage systematic sampling and a standardized semi-structured questionnaire. Nine key informant interviews were conducted through purposive selection of health care providers and members of Village Health Teams (VHTs) based on their expertize. Modified Poisson regression and thematic content analysis were used to determine factors significant to IPV uptake among children. 71% of sampled children aged 12–23 months had received IPV in Kalungu District. The survey found that being encouraged by health workers and VHTs was significant to children’s uptake of IPV (Adjusted PR 1.24, 95% CI; 1.22–3.47). Distance to the immunization point (Adjusted PR 0.32,95% CI; 0.16–0.62) and caregiver’s education level (Adjusted PR 1.16,95% CI; 1.05–2.22) were also associated with IPV uptake. Qualitative findings from health workers and VHT members further confirmed the perception that distance to the immunization post was important, and VHTs also stated that being encouraged by health workers was critical to IPV uptake. The current prevalence of IPV uptake among children aged 12–23 months in Kalungu is 71%, higher than the last reported national coverage (60%), though still below the recommended national coverage of 95%. Efforts should be focused on sensitization of caregivers through health workers and VHTs. Immunization outreach should be strengthened so as to bring services closer to patients.

scholarly journals Tingkat Pengetahuan Mahasiswa Fakultas Kedokteran Universitas Yarsi Mengenai Penggunaan Human Diploid Cell Dalam Proses Produksi Vaksin MR Dan Tinjauannya Menurut Islam

2021 ◽  
Vol 1 (12) ◽  
Author(s):  
Nurfatimah Aprilianda Simatupang ◽  
Indra Kusuma ◽  
Siti Nur Riani
Keyword(s):  
Random Sampling ◽  
Simple Random Sampling ◽  
Diploid Cell ◽  
Human Diploid Cell ◽  
Cross Sectional

Latar Belakang :  Vaksin MR merupakan salah satu vaksin yang wajib diberikan kepada anak 0 – 9 bulan. Produksi vaksin MR menggunakan Human Diploid Cell yang berasal dari janin yang sengaja di abortus menimbulkan kontroversi mengenai halal dan haram vaksin MR. Penggawa kesehatan masyarakat musti memahami dan memiliki dasar keilmuan untuk dapat menjawab kerisauan dan kontroversi mengenai kehalalan vaksin MR sehingga masyarakat menerima penggunaan vaksin MR sebagai bentuk preventif dari penyakit Measles. Menurut pandangan Islam, vaksin MR hukumnya mubah karena prinsip dharuriyah untuk menjaga hifdz nasb dan hifdz nafs. Metode : Jenis penelitian yang digunakan adalah penelitian deskriptif dengan pendekatan cross sectional menggunakan kuesioner. Populasi penelitian adalah mahasiswa fakultas kedokteran Universitas YARSI tahun pertama dan tahun ketiga yang memenuhi definisi operasional yang dipilih dengan teknik simple random sampling.  Hasil : Penelitian yang dilaksanakan menggunakan kuesioner didapatkan dari 100 responden. Persentase jumlah kuesioner Pengetahuan  mengenai Human Diploid Cell  berdasarkan Tingkat Pendidikan didapatkan pengetahuan baik sebanyak 13,51% pada tahun ketiga dan 11,11% pada tahun pertama. Pengetahuan cukup sebanyak 62,16% pada tingkat ketiga dan 44,44% pada tahun pertama. Pengetahuan kurang sebanyak 24,32% pada tingkat ketiga dan 44,44% pada tahun pertama . Persentase jumlah kuesioner Pengetahuan  mengenai Vaksin MR berdasarkan Tingkat Pendidikan didapatkan pengetahuan baik sebanyak 54,05% pada tahun ketiga dan 49,21% pada tahun pertama. Pengetahuan cukup sebanyak 43,24% pada tingkat ketiga dan 42,86% pada tahun pertama. Pengetahuan kurang sebanyak 2,70% pada tingkat ketiga dan 7,94% pada tahun pertama. Simpulan :  Tidak terdapat hubungan antara Tingkat Pendidikan dengan pengetahuan mengenai Penggunaan Human Diploid Cell dalam Proses Produksi Vaksin MR. Menurut Islam, penggunaan vaksin MR menjadi mubah sebagai upaya menegakkan prinsip dharuriyah dalam menjaga keturunan bagi orang tua (hifdz nasb) dan menjaga nyawa anak dari ancaman penyakit Measles (hifdz nafs).

The growth of Trypanosoma cruzi in human diploid cells for the production of trypomastigotes

Parasitology ◽  
1980 ◽  
Vol 80 (1) ◽  
pp. 153-162 ◽  
Author(s):  
C. J. Sanderson ◽  
Jennifer A. Thomas ◽  
Cheryl E. Twomey
Keyword(s):  
Trypanosoma Cruzi ◽  
Culture Medium ◽  
Diploid Cell ◽  
Human Diploid Cell ◽  
Cell Debris ◽  
Single Strain ◽  
Passage Number ◽  
Human Diploid Cells ◽  
Diploid Cells

SummaryThe use of human diploid cell lines of finite life for the in vitro production of Trypanosoma cruzi is described. Both MRC5 and WI38 cells release trypomastigotes with less than 5% amastigotes. This could form the basis for biochemical and immunological studies, which were previously limited by the problems of separating parasites from blood. By selecting the in vitro passage number of the parasite it is possible to select for either the broad or the slender forms of trypomastigotes, allowing comparative studies of these forms within a single strain of the parasite. It is also possible to isolate amastigotes by disrupting the cells before trypomastigotes appear, and separating them from cell debris with Metrizamide. It is shown that by incorporating [3H]uridine in the cell-culture medium, labelled trypomastigotes are obtained. The release of this label (putative RNA) provides a relatively simple isotopic assay for parasite death. Examples of this assay for testing drug toxicity and in immunological lysis are presented.

scholarly journals Impact of Maternal Antibody on the Immunogenicity of Inactivated Polio Vaccine in Infants Immunized With Bivalent Oral Polio Vaccine: Implications for the Polio Eradication Endgame

2018 ◽  
Vol 67 (suppl_1) ◽  
pp. S57-S65 ◽  
Author(s):  
James T Gaensbauer ◽  
Chris Gast ◽  
Ananda S Bandyopadhyay ◽  
Miguel O’Ryan ◽  
Xavier Saez-Llorens ◽  
...  
Keyword(s):  
Oral Polio Vaccine ◽  
Polio Eradication ◽  
Maternal Antibody ◽  
Inactivated Polio Vaccine ◽  
Polio Vaccine

scholarly journals Failure to detect infection by oral polio vaccine virus following natural exposure among inactivated polio vaccine recipients

2007 ◽  
Vol 136 (2) ◽  
pp. 180-183 ◽  
Author(s):  
H. E. GARY ◽  
B. SMITH ◽  
J. JENKS ◽  
J. RUIZ ◽  
W. SESSIONS ◽  
...  
Keyword(s):  
Oral Polio Vaccine ◽  
Developed Countries ◽  
The United States ◽  
Vaccine Virus ◽  
Susceptible Population ◽  
Inactivated Polio Vaccine ◽  
Polio Vaccine ◽  
Natural Exposure ◽  
The Us ◽  
Protection Against Infection

SUMMARYWhile oral polio vaccine (OPV) has been shown to be safe and effective, it has been observed that it can circulate within a susceptible population and revert to a virulent form. Inactivated polio vaccine (IPV) confers protection from paralytic disease, but provides limited protection against infection. It is possible, then, that an IPV-immunized population, when exposed to OPV, could sustain undetected circulation of vaccine-derived poliovirus. This study examines the possibility of polio vaccine virus circulating within the United States (highly IPV-immunized) population that borders Mexico (OPV-immunized). A total of 653 stool and 20 sewage samples collected on the US side of the border were tested for the presence of poliovirus. All samples were found to be negative. These results suggest that the risk of circulating vaccine-derived poliovirus is low in fully immunized IPV-using populations in developed countries that border OPV-using populations.

Parental acceptance of inactivated polio vaccine in Southeast Nigeria: A qualitative cross-sectional interventional study

Vaccine ◽  
2014 ◽  
Vol 32 (46) ◽  
pp. 6157-6162 ◽  
Author(s):  
Beckie Nnenna Tagbo ◽  
Maduka Donatus Ughasoro ◽  
Dorothy Omono Esangbedo
Keyword(s):  
Parental Acceptance ◽  
Interventional Study ◽  
Cross Sectional ◽  
Inactivated Polio Vaccine ◽  
Polio Vaccine

scholarly journals Polio inactivated vaccine costs into routine childhood immunization in Brazil

2015 ◽  
Vol 49 (0) ◽  
Author(s):  
Ana Marli Christovam Sartori ◽  
Margarete Paganotti Vicentine ◽  
Lígia Castelloni Figueiredo Gryninger ◽  
Patricia Coelho de Soárez ◽  
Hillegonda Maria Dutilh Novaes
Keyword(s):  
Oral Vaccine ◽  
Oral Polio Vaccine ◽  
Inactivated Vaccine ◽  
Childhood Immunization ◽  
Immunization Program ◽  
Inactivated Polio Vaccine ◽  
Polio Vaccine ◽  
National Immunization ◽  
National Immunization Day ◽  
The Cost

OBJECTIVE To analyze the costs of vaccination regimens for introducing inactivated polio vaccine in routine immunization in Brazil.METHODS A cost analysis was conducted for vaccines in five vaccination regimens, including inactivated polio vaccine, compared with the oral polio vaccine-only regimen. The costs of the vaccines were estimated for routine use and for the “National Immunization Days”, during when the oral polio vaccine is administered to children aged less than five years, independent of their vaccine status, and the strategic stock of inactivated polio vaccine. The presented estimated costs are of 2011.RESULTS The annual costs of the oral vaccine-only program (routine and two National Immunization Days) were estimated at US$19,873,170. The incremental costs of inclusion of the inactivated vaccine depended on the number of vaccine doses, presentation of the vaccine (bottles with single dose or ten doses), and number of “National Immunization Days” carried out. The cost of the regimen adopted with two doses of inactivated vaccine followed by three doses of oral vaccine and one “National Immunization Day” was estimated at US$29,653,539. The concomitant replacement of the DTPw/Hib and HepB vaccines with the pentavalent vaccine enabled the introduction of the inactivated polio without increasing the number of injections or number of visits needed to complete the vaccination.CONCLUSIONS The introduction of the inactivated vaccine increased the annual costs of the polio vaccines by 49.2% compared with the oral vaccine-only regimen. This increase represented 1.13% of the expenditure of the National Immunization Program on the purchase of vaccines in 2011.

scholarly journals The Pathology of Poliomyelitis and the Vaccines and Nonvaccine Therapy

2021 ◽  
Vol 308 ◽  
pp. 02018
Author(s):  
Yushuo Chen ◽  
Tianrui Yue ◽  
Zixiao Zhang
Keyword(s):  
Developing Countries ◽  
Nervous System ◽  
Low Cost ◽  
Oral Polio Vaccine ◽  
Polio Eradication ◽  
Paralytic Poliomyelitis ◽  
Strongly Correlated ◽  
Inactivated Polio Vaccine ◽  
Polio Vaccine ◽  
New Type

Poliomyelitis is an exclusively human disease that mainly affects children. Clinical features of poliomyelitis can be varied, from mild illness to the most severe paralysis, and the factor why poliomyelitis has different performances in individuals has been proved strongly correlated with membrane protein CD155. The nervous system shows a special protecting phenomenon against the invasion of poliovirus, and the mechanism is not very clear at present. Vaccines are the main means of preventing and controlling polio, and many different vaccines have been invented in the process of fighting polio. Inactivated polio vaccine (IPV) and oral polio vaccine (OPV) are the two main vaccines. IPV is known for its safety while OPV is widely used in developing countries because of its relatively low cost. This usage also leads to some side effects: vaccine-associated paralytic polio (VAPP) and vaccine-derived poliovirus (VDPV). Now, for polio eradication, the elimination of these two diseases has become particularly important. Thus, a new type of vaccine was created: sequential IPV-OPV with the safety of IPV and the low cost of OPV. This paper will talk about the different polio vaccines and their effects. An enormous difference between people who have gotten the vaccine and people who have not got the vaccine. Comparing the two kinds of people, people who get normal poliovirus, and people who get poliovirus after taking a vaccine, known as VAPP (vaccine-associated paralytic poliomyelitis), the former cannot get full recovery whole life and the latter has a very low possibility. In conclusion, people should take vaccines if it is affordable for them.

scholarly journals Demonstration of dual rhinovirus infection in humans by isolation of different serotypes in human heteroploid (HeLa) and human diploid fibroblast cell cultures

1977 ◽  
Vol 5 (2) ◽  
pp. 202-207
Author(s):  
M K Cooney ◽  
G E Kenny
Keyword(s):  
Hela Cell ◽  
Cell Cultures ◽  
Hela Cells ◽  
Fibroblast Cell ◽  
Diploid Cell ◽  
Human Diploid Cell ◽  
Human Diploid Fibroblast ◽  
First Passage ◽  
Cytopathic Effects ◽  
Diploid Cells

The ability to isolate rhinoviruses in human heteroploid cell cultures was investigated by inoculating HeLa cells (HeLa M) with specimens previously shown to be positive in human diploid cell cultures. The 135 positive specimens selected were representative of 22 different rhinovirus types, and 4 to 9 specimens were available for each serotype. Specimens were inoculated into human diploid fetal tonsil fibroblasts (FT), HeLa cells with 30 mM Mg2+, and HeLa cells without increased Mg2+. One hundred twelve rhinovirus strains (83%) were reisolated in FT cells, whereas 76 rhinovirus strains (56%) were recovered in HeLa cells with 30 mM Mg2+. All strains recovered in FT were the same serotype as that originally recovered in diploid cells, but five of the HeLa cell isolates (3.7% of total specimens) were different serotypes, indicating dual rhinovirus infections. Four rhinovirus serotypes, (3, 42, 48, and 70) were recovered in HeLa but not in diploid cells; these serotypes were rare in our previous studies. Isolation of rhinovirus in FT cells was usually accomplished at first passage, whereas rhinovirus cytopathic effects in HeLa cells were not observed at first passage, but required one, two, or (rarely) three blind passages. Only 28 rhinoviruses (21%) were recovered in HeLa cells without increased Mg2+; however, three serotypes, types 16, 36, and 58, were recovered as effectively in HeLa cells, with or without added Mg2+, as they were in FT cells. In general, rhinoviruses were less efficiently recovered in HeLa cells; however, certain serotypes may be detected better by HeLa cells.

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