scholarly journals ЦИТОМЕТРИЧЕСКИЕ ПОКАЗАТЕЛИ ЭПИТЕЛИАЛЬНОГО СЛОЯ ЯЙЦЕВОДА КУР В ВОЗРАСТНОМ ИНТЕРВАЛЕ 30-120 СУТОК

2019 ◽  
pp. 109-114
Author(s):  
R.Yu. Khokhlov ◽  
S.I. Kuznetsov
Keyword(s):  
Epithelial Cells ◽  
Mucous Membrane ◽  
Egg Laying ◽  
Epithelial Layer ◽  
Caudal Part ◽  
Minimum Area ◽  
Maximum Area ◽  
Caudal Region

Статья посвящена изучению цитометрических показателей эпителиального слоя слизистой оболочки яйцевода кур до начала яйцекладки, а именно с 30- по 120-суточный возраст. В результате анализа полученных цитометрических показателей установлено, что толщина эпителиального слоя краниального отдела яйцевода поступательно увеличивается от 30- до 90-суточного возраста. В каудальной части яйцевода аналогичная тенденция прослеживается на месяц позже, то есть с 60-суточного возраста. Наибольшие ядра эпителиоциты краниального отдела имели в 90-суточном возрасте, а в каудальном в 120-суточном возрасте. Размеры эпителиальных клеток в краниальном отделе менялись от минимальных значений в 30-суточном возрасте до максимальных в 60-суточном возрасте. В каудальном отделе минимальная площадь клеток эпителия отмечается, равно как и в краниальном, в 30-суточном возрасте. Однако, максимальная площадь эпителиоцитов в каудальном отделе не совпадает с краниальным отделом, так как наиболее крупные эпителиальные клетки в каудальном отделе отмечаются в 120-суточном возрасте. Минимальное отношение ядер к цитоплазме в краниальной части яйцевода отмечалось в 60-суточном возрасте, а в каудальной в 90-суточном возрасте. Наибольшее ядерно-цитоплазматическое отношение в краниальной части зафиксировано в 30-суточном возрасте, а в каудальной в 30- и 120-суточном возрасте.The article is devoted to the study of cytometric parameters of the epithelial layer of the mucous membrane of the oviduct of hens before the start of egg-laying, namely from 30 to 120 days of age. As a result of the analysis of the obtained cytometric indicators, it was found that the thickness of the epithelial layer of the cranial part of the oviduct progressively increases from 30 to 90 days of age. A similar trend is observed in the caudal part of the oviduct a month later, that is, from 60 days of age. Cranial epithelial cells had the largest nuclei at the age of 90 days, and caudal - at the age of 120 days. The sizes of epithelial cells in the cranial region varied from the minimum values at the age of 30 days to the maximum values at the age of 60 days. The minimum area of epithelial cells is noted in the caudal region, as well as in the cranial, at the age of 30 days. However, the maximum area of epithelial cells in the caudal region does not coincide with the cranial region, since the largest epithelial cells in the caudal region are observed at 120 days of age. The minimal ratio of nuclei to the cytoplasm in the cranial part of the oviduct was observed at 60 days of age, and in the caudal - at 90 days of age. The highest nuclear cytoplasmic ratio in the cranial part was recorded at 30 days of age, and in the caudal part - at 30 and 120 days of age.

Electron Microscopic Study of the Epithelium of the Seminal Vesicle of Aging Rats

1974 ◽  
Vol 32 ◽  
pp. 138-139
Author(s):  
V. F. Allison ◽  
G. C. Fink ◽  
G. W. Cearley
Keyword(s):  
Cell Growth ◽  
Epithelial Cell ◽  
Epithelial Cells ◽  
Seminal Vesicle ◽  
Seminal Vesicles ◽  
Epithelial Layer ◽  
Epithelial Hyperplasia ◽  
Electron Microscopic ◽  
Aging Rats ◽  
Pseudostratified Epithelium

It is well known that epithelial hyperplasia (benign hypertrophy) is common in the aging prostate of dogs and man. In contrast, little evidence is available for abnormal epithelial cell growth in seminal vesicles of aging animals. Recently, enlarged seminal vesicles were reported in senescent mice, however, that enlargement resulted from increased storage of secretion in the lumen and occurred concomitant to epithelial hypoplasia in that species.The present study is concerned with electron microscopic observations of changes occurring in the pseudostratified epithelium of the seminal vescles of aging rats. Special attention is given to certain non-epithelial cells which have entered the epithelial layer.

scholarly journals MORPHOLOGY AND MORPHOMETRIC INDICATORS OF THE MUCOUS MEMBRANE OF VARIOUS PARTS OF THE INTESTINE OF PATIENTS WITH POSTCHOLECYSTECTOMY SYNDROME

2020 ◽  
Vol 28 (3) ◽  
pp. 58-64
Author(s):  
Ivan T. Shcherbakov ◽  
Nina I. Leontieva ◽  
Nina M. Grachiova ◽  
Alina I. Soloviova ◽  
Nikolay A. Vinogradov ◽  
...  
Keyword(s):  
Sigmoid Colon ◽  
Mucous Membrane ◽  
Recurrent Abdominal Pain ◽  
Pathological Process ◽  
Morphometric Study ◽  
Control Group ◽  
Epithelial Layer ◽  
Intestinal Villi ◽  
Postcholecystectomy Syndrome ◽  
Number Of Patients

The actuality of the problem is associated with an increase in the number of patients with cholelithiasis of working age and a significant increase in cholecystectomies not only in Russia, but throughout the world. It is known that more than 2,5 million are performed annually. Moreover, 5-40% of operated patients develop postcholecystectomy syndrome, which is manifested by recurrent abdominal pain and cholegenic diarrhea. The aim of the study is to assess morphofunctional changes in the mucous membrane of various parts of the intestine in patients with postcholecystectomy syndrome. Histological, histochemical, morphometric and bacterioscopic, parasitological methods were used to study 42 biopsy specimens of the mucous membrane of various parts of the intestines of patients who underwent cholecystectomy. The control group consisted of 18 biopsies of practically healthy individuals. To objectify the study, a morphometric study of the mucous membrane of the duodenum according to 34 parameters, of the cecum and sigmoid colon according to 22 parameters was carried out. In the mucous membrane of the duodenum, chronic duodenitis of varying degrees of activity of the pathological process and atrophy of the intestinal villi were revealed, the intestinal glands were shortened, the intestinal villi are less wide. The epithelial layer of intestinal villi and intestinal glands was abundantly infiltrated with neutrophilic granulocytes. The density of the inflammatory cellular infiltrate in the stroma of the intestinal villi and intestinal glands increased. On the surface of the epithelial layer of the intestinal villi and between the intestinal glands, campylobacters were often found, less often - cryptosporidia. In the mucous membrane of the cecum and sigmoid colon, chronic colitis of varying degrees of activity of the pathological process and atrophy of the intestinal glands were revealed. The thickness of the mucous membrane and the depth of the intestinal glands were lower than normal. Thus, in patients with postcholecystectomy syndrome, pronounced morphometric and histological changes were revealed in the mucous membrane of various parts of the intestine, while the depth and degree of its damage were more significant in its proximal parts.

Peculiarities of the Gingiva Cellular Composition and Functional Activity of the Epithelial Cells among the Children Patients with Chronic Gastritis and Duodenitis

2016 ◽  
Vol 29 ◽  
pp. 54-60
Author(s):  
Y.G. Romanenko ◽  
A.D. Matveeva
Keyword(s):  
Candida Albicans ◽  
Epithelial Cells ◽  
Functional Properties ◽  
Chronic Gastritis ◽  
Functional Property ◽  
Functional Activity ◽  
Polymorphonuclear Leukocytes ◽  
Epithelial Layer ◽  
Cellular Composition ◽  
Skin Cells

At the children with chronic gastritis and duodenitis was revealed destroyed of morphological - functional property of gums with prevalence of degenerative component, which is manifested by appearance of basal and parabasal cells (0.3%), increasing of intermediate epithelial cells (84.7%), decreasing amount of surface nucleated epithelial cells (11.7%), and nuclear-free dead skin cells (4.3%). Majority of cells was with cytopathology shifts (18.2%), polymorphonuclear leukocytes (92.3%) and lymphocytes (20.5%). The data obtained in the research showed that 23 (21.5%) of samples, taken from children with chronic gastritis and duodenitis revealed cells in the early stages of differentiation, accumulation of leukocytes and fibrin, which demonstrates erosions of the gums in the epithelial layer. Adhesion of epithelial cells with Candida albicans at the children with chronic gastritis and duodenitis indicates about changes functional properties of the epithelial cells, growing on a background of the somatic pathology.

scholarly journals Redistribution of the tight junction protein ZO-1 during physiological shedding of mouse intestinal epithelial cells

2011 ◽  
Vol 300 (6) ◽  
pp. C1404-C1414 ◽  
Author(s):  
Yanfang Guan ◽  
Alastair J. M. Watson ◽  
Amanda M. Marchiando ◽  
Emily Bradford ◽  
Le Shen ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Tight Junction ◽  
Cell Nucleus ◽  
Total Duration ◽  
Immunofluorescent Staining ◽  
Cell Renewal ◽  
Epithelial Layer ◽  
Fixed Tissue ◽  
Junction Protein ◽  
Cell Shedding

We questioned how tight junctions contribute to intestinal barrier function during the cell shedding that is part of physiological cell renewal. Intravital confocal microscopy studied the jejunal villus epithelium of mice expressing a fluorescent zonula occludens 1 (ZO-1) fusion protein. Vital staining also visualized the cell nucleus (Hoechst staining) or local permeability to luminal constituents (Lucifer Yellow; LY). In a cell fated to be shed, ZO-1 redistributes from the tight junction toward the apical and then basolateral cell region. ZO-1 rearrangement occurs 15 ± 6 min ( n = 28) before movement of the cell nucleus from the epithelial layer. During cell extrusion, permeation of luminal LY extends along the lateral intercellular spaces of the shedding cell only as far as the location of ZO-1. Within 3 min after detachment from the epithelial layer, nuclear chromatin condenses. After cell loss, a residual patch of ZO-1 remains in the space previously occupied by the departed cell, and the size of the patch shrinks to 14 ± 2% ( n = 15) of the original cell space over 20 min. The duration of cell shedding measured by nucleus movement (14 ± 1 min) is much less than the total duration of ZO-1 redistribution at the same sites (45 ± 2 min). In about 15% of cell shedding cases, neighboring epithelial cells also undergo extrusion with a delay of 5–10 min. With the use of normal mice, ZO-1 immunofluorescent staining of fixed tissue confirmed ZO-1 redistribution and the presence of ZO-1 patches beneath shedding cells. Immunostaining also showed that redistribution of ZO-1 occurred without corresponding mixing of apical and basolateral membrane domains as marked by ezrin or E-cadherin. ZO-1 redistribution is the earliest cellular event yet identified as a herald of physiological cell shedding, and redistribution of tight junction function along the lateral plasma membrane sustains epithelial barrier during cell shedding.

scholarly journals RISK FACTORS FOR MALIGNANCY OF PAPILLOMAS IN THE OROPHARYNGEAL MUCOUS MEMBRANE ASSOCIATED WITH HUMAN PAPILLOMAVIRUS (HPV)

2021 ◽  
Vol 11 (3) ◽  
pp. 46-49
Author(s):  
Galina Reva ◽  
Stanislav Ichenko ◽  
Igor Sementsov ◽  
Aleksandr Kim ◽  
Oksana Voskanyan ◽  
...  
Keyword(s):  
Mucous Membrane ◽  
Process Development ◽  
Basal Membrane ◽  
Growth Zone ◽  
Pathological Process ◽  
Oral Epithelium ◽  
Epithelial Layer ◽  
Third Stage ◽  
Hematoxylin And Eosin ◽  
Antigen Presenting

Analysis of the HPV positive papilloma structures at different levels of pathological process development was carried out. Classical staining of preparations with hematoxylin and eosin was used for immunohistochemical determination of Ki67-positive cells and phenotyping of CD positive cells. We found that the process of papilloma formation begins with a local increase in the proliferative activity of keratinocytes which contributes to the formation of a local epithelial convex above the surface of the oropharyngeal mucosa (OPM) in the form of papilloma. The connective tissue of the OPM adjacent to the epithelium grows at the second stage. At the same time here is a decrease in immunocytes/macrophages number of the oral epithelium not only in the growth zone of the papilloma but in the adjacent neoplasm tissue. The third stage is characterized by the destruction of the basal membrane of the OPM. Apoptotic cells in the cambial layer and forming leukocytes infiltrate the OPM lamina propria. Virus-infected keratinocytes are phagocytized by macrophages or exfoliated from the surface of the epithelial layer. The emerging in the middle layers defect of tissue of the epithelial layer and the absence of Langerhans cells indicate a relationship between the migration of antigen-presenting cells expressing CD68 with impaired differentiation and specialization of keratinocytes. The conclusion is based on the analogy of "leukemic failure" in leukemia and on the absence of differentiating epithelial cells between the cambium and the specialized surface layer in the long-existing papilloma. The disappearance from the epithelium of CD68-positive cells specific to the epithelial layer is a prognostic sign of malignization in the mucous membrane of the oropharynx.

scholarly journals Features of the Expression of Second-Type Melatonin Receptors by Esophagus Epiteliocytes in Gastroesophageal Reflux Disease

2020 ◽  
Vol 30 (2) ◽  
pp. 26-34
Author(s):  
O. A. Karpovich ◽  
T. T. Shtabinskaya ◽  
V. I. Shishko ◽  
Ya. A. Kolodzeysky
Keyword(s):  
Gastroesophageal Reflux Disease ◽  
Epithelial Cells ◽  
Gastroesophageal Reflux ◽  
Mucous Membrane ◽  
Reflux Disease ◽  
Melatonin Receptors ◽  
Control Group ◽  
Expression Intensity ◽  
Low Intensity ◽  
Esophageal Epithelial Cells

Aim. To investigate the specific features of the expression of second-type melatonin receptors (MTNR1B) by epithelial cells of the distal esophagus in gastroesophageal reflux disease (GERD), depending on the severity of endoscopic changes in the mucous membrane.Materials and methods. The study included 48 GERD patients, out of whom 37 and 11 people were suffering from non-erosive reflux disease (NERD) and erosive reflux disease (ERD), respectively. The control group consisted of 18 patients without GERD, comparable with the main groups by gender, age and body mass index. In order to determine the expression of MTNR1B, esophagogastroduodenoscopy with the collection of biopsy material from the mucous membrane of the distal esophagus was performed. The quantification of the MTNR1B expression intensity was carried out using the Aperio ImageScope_v9.1.19.1567 software. The level of melatonin metabolite, 6-sulphatehydroxymelatonin (6-SOMT), was determined in daily urine, as well as separately in daytime and nighttime portions.Results. The intensity of MTNR1B expression by esophageal epithelial cells in patients with NERD demonstrated no difference with the control group (0.436 (0.123, 0.668) and 0.437 (0.202, 0.692), respectively; p> 0.05). A significant decrease in the expression of MTNR1B receptors was noted in patients with ERD compared to NERD patients (0.127 (0.059, 0.156) and 0.436 (0.123, 0.668), respectively; p = 0.017) and patients in the control group (0.437 (0.202, 0.692); p = 0.033). The low intensity of MTNR1B expression was associated with more pronounced endoscopic changes in the mucous membrane of the esophagus (r = –0.40; p = 0.0015). A statistically significant relationship was found between the expression intensity of MTNR1B and the level of melatonin in the daytime (r = 0.42; p = 0.018), as well as the night/day index reflecting the daily rhythm of melatonin synthesis (r = –0.43; p = 0.016).Conclusions. The obtained data indicate that a decrease in the intensity of MTNR1B expression by esophageal epithelial cells can be considered as a prognostically unfavourable sign of the GERD course. The low intensity of MTNR1B expression is associated with a more severe (erosive) form of GERD and more pronounced endoscopic changes in the mucous membrane of the esophagus.

The ultrastructual morphology of the midgut diverticulum of the calanoid copepod Calanus helgolandicus (Claus) (Crustacea)

1970 ◽  
Vol 18 (1) ◽  
pp. 9 ◽  
Author(s):  
JE Ong ◽  
PS Lake
Keyword(s):  
Amino Acids ◽  
Epithelial Cells ◽  
Myoepithelial Cell ◽  
Calanoid Copepod ◽  
Apical Region ◽  
Basal Region ◽  
Epithelial Layer ◽  
Apical Half ◽  
Mammalian Stomach ◽  
Blue Reaction

The midgut diverticulum of the marine calanoid copepod C. helgolandicus consists of a columnar epithelial layer, a myoepithelial layer, and between these a well-developed basement membrane. The apical region of the epithelial cell is thrown into tightly packed microvilli which showed an alcian blue reaction indicating the presence of acid mucopolysaccharides. The apical half of the cell contains numerous microvesicles and mitochondria as well as tiny Golgi-like bodies. The plasma membrane of the basal region of the epithelium is extremely digitated. The digitations contain numerous mitochondria and the whole structure resembles mitochondrial pumps. The epithelial cells contain a large centrally situated oval nucleus with its single nucleolus. The myoepithelial cell is squamous and contains a flattened nucleus as well as very well-developed circularly and longitudinally arranged myofibrils. It is suggested that the midgut diverticulum of Calanus is probably analogous to the mammalian stomach in that food is mechanically churned. However, it does not appear to be involved in the secretion of digestive juices but only mucopolysaccharides; it is probably involved in the absorption of amino acids which are probably actively transported, by the "mitochondrial pump" in the basal region of the epithelial cells, into the haemocoel.

A Histochemical Study of Fat Absorption in the Small Intestine of the Rat

1954 ◽  
Vol s3-95 (30) ◽  
pp. 153-158
Author(s):  
W. HEWITT
Keyword(s):  
Small Intestine ◽  
Epithelial Cells ◽  
Mucous Membrane ◽  
Histochemical Study ◽  
Fat Absorption ◽  
Red Coloration

Experiments are described in which sections from the small intestine of rats, fed on triolein 2¾ hours previously, were examined with Sudan black and the acid haematein test, with and without Sudan red coloration. The histochemical appearances in the epithelial cells of the mucous membrane are described. Lipoid can be observed not only within the epithelial cells and their free border but also as particles between the cells.

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