scholarly journals LC-ESI-MS/MS analysis, toxicity and anti-anaemic activity of Rubia tinctorum L. aqueous extract

2022 ◽  
pp. e978
Author(s):  
Fatima Zohra Houari ◽  
Ramazan Erenler ◽  
Sena Bakir ◽  
Esra Capanoglu ◽  
Ahmed Hariri
Keyword(s):  
Single Dose ◽  
Citric Acid ◽  
Aqueous Extract ◽  
Root Extract ◽  
Acute Toxicity Test ◽  
Chemical Profile ◽  
Esi Ms ◽  
Rubia Tinctorum ◽  
Ms Analysis ◽  
Phytochemical Compounds

The present study investigated the chemical profile, toxicity, and anti-anaemic activity of Rubia tinctorum root aqueous extract against phenylhydrazine induced hemolytic anaemia. Phenolic compounds were analyzed by LC-ESI-MS/MS; acute toxicity test was evaluated by administering a single dose of 2,000 mg.kg-1 of the extract; anaemia was induced by administration of 40 mg phenylhydrazine by intraperitoneal injection for 2 days. Moreover, the anti-anaemic activity was evaluated by measuring the haematological parameters of rats treated with iron and aqueous extract for 15 days. The LC-ESI-MS/MS analysis results revealed the presence of 31 phytochemical compounds, among them, citric acid was found as the most abundant. No signs of toxicity or death were recorded, indicating that the LD50 of R. tinctorum root extract is higher than 2,000 mg.kg-1. Furthermore, the aqueous extract increased red blood cell levels by 69.82 and 71.67 % in the groups treated with 200 and 400 mg.kg-1 of the extract, respectively. Besides, a significant increase in hemoglobin from 12.05 ± 0.15 to 12.9 ± 0.52 g.dL-1 was noted in rats treated with 400 mg.kg-1 of extract. Thus, the data indicate that the root extract could be considered a natural source for the treatment of anaemia.

One single HPLC-PDA/(-)ESI-MS/MS analysis to simultaneously determine 30 components of the aqueous extract of Rabdosia rubescens

2011 ◽  
Vol 879 (26) ◽  
pp. 2783-2793 ◽  
Author(s):  
Jingcheng Tang ◽  
Ming Zhao ◽  
Yuji Wang ◽  
Guifeng Kang ◽  
Jianhui Wu ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Esi Ms ◽  
Ms Analysis ◽  
Rabdosia Rubescens

scholarly journals Screening and Evaluation of Bioactive Components of Methanol Root Extract of Anthocleista nobilis G. Don. by GCMS Analysis

2020 ◽  
Author(s):  
Adekunle ORIMISAN Ojatula
Keyword(s):  
Methanol Extract ◽  
Biological Activities ◽  
Biologically Active ◽  
Root Extract ◽  
Bioactive Constituents ◽  
Active Metabolites ◽  
Ms Analysis ◽  
Medicinal Value ◽  
Study Results ◽  
Phytochemical Compounds

Abstract Background: Medicinal plants have great importance in African medicine and are also used as precursors in drug discovery. The medicinal value of plants lies in their bioactive constituents which usually allow them to fight against several diseases. Plant-based natural constituents can be derived from any part of the plant like roots, bark, leaves, flowers, fruits, seeds. The present study was designed to determine the bioactive/biochemical compounds present in the methanol extract of Anthocleista nobilis root.Materials and Methods: GC -MS analysis of the methanol extract of A. nobilis root was performed using a Perkin Elmer GC Clarus 500 system comprising an Agilent technologies 5975 MSD model detector and a gas chromatograph interfaced to a mass spectrometer with the aid of the Turbo mass 5.0 software.Results: The study results of the GC-MS analysis provided different phytochemical compounds possessing several biological activities such as antimicrobial, antifungal, anticancer, and anti-inflammatory activities. This study therefore, showed that root of Anthocleista nobilis is a source of biologically active metabolites. Furthermore, root extract revealed the presence of diverse chemical constituents.Conclusion: The results of the present study suggest a recommendation of A. nobilis root as a plant of phytopharmaceutical importance.

LC-ESI-MS/MS analysis of Quassia amara leaves tea. Is antiplasmodial activity of the tea is due to quassinoids?

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
N Fabre ◽  
E Deharo ◽  
HL Le ◽  
C Girardi ◽  
A Valentin ◽  
...  
Keyword(s):  
Antiplasmodial Activity ◽  
Esi Ms ◽  
Ms Analysis ◽  
Quassia Amara

FTIR, GC-MS and HPLC analysis of Baliospermum montanum (Willd.) Muell. Arg.

2020 ◽  
Vol 11 (4) ◽  
pp. 5059-5066
Author(s):  
Sushma B K ◽  
Raveesha H R
Keyword(s):  
Acetic Acid ◽  
Quantitative Estimation ◽  
Chemical Constituents ◽  
Standard Procedure ◽  
Naphthalene Acetic Acid ◽  
Root Extract ◽  
Pentanoic Acid ◽  
Trimethylsilyl Ester ◽  
Isolation And Characterization ◽  
Ms Analysis

The present work is aimed to determine the chemical constituents in Baliospermum montanum methanolic extracts. An in vitro regenerated procedure was developed for the induction of callus from stem explant cultured on Murashige and Skoog (MS) medium fortified with various concentration and permutations of 2, 4-dichloro phenoxy acetic acid, 1-naphthalene acetic acid, 6-benzyl amino purine and gibberellic acid. FTIR & GC-MS analysis was done according to standard procedure. The quantitative estimation of β-sitosterol was done by HPLC method. Maximum fresh and dry weight of callus was estimated in the combination of GA3 (0.5 mg/L) + NAA (2 mg/L) compared to other concentration. The FTIR analysis showed various functional compounds with different characteristic peak values in the extracts. Major bioactive constituents were recognized in the GC-MS analysis. Root extract revealed the existence of 1-hexadecanol, pentanoic acid, 2-(aminooxy)- and 1-hexacosanol. Leaf extract showed the presence of propanoic acid, 2-oxo-, trimethylsilyl ester, 9,12-octadecadienoic acid (z,z)-, trimethylsilyl ester, docosane, 1,22-dibromo- and pentatriacontane. Stem and stem derived callus exhibit the presence of 1,6,3,4-dihydro-2-deoxy-beta-d-lyxo-hexopyranose, n-hexadecanoic acid and pentanoic acid, 2-(aminooxy). The methanolic extract of leaf exhibited 0.2149 % of β-sitosterol content. There were no peaks observed in the root, stem and stem derived callus. Further studies are necessary for the isolation and characterization of bioactive compounds from B. montanum.

Valorization of the Pharmacological Potential of Phytochemical Compounds Contained in the Crude Extract of the Root of a Plant of Withania frutescens L

2019 ◽  
Author(s):  
A. EL Moussaoui ◽  
F. Jawhari ◽  
K. EL Ouahdani ◽  
I. Es-Safi ◽  
D. Bousta ◽  
...  
Keyword(s):  
Crude Extract ◽  
Control Group ◽  
Root Extract ◽  
Nacl Solution ◽  
Phytochemical Compounds ◽  
Anti Inflammatory ◽  
Pharmacological Potential

Our present study focuses on the evaluation of the analgesic, anti-inflammatory and healing activity of Withania frutescens L. The anti-inflammatory result has an inhibition percentage of 78.87% ± 7.08 at 450 mg/kg and 75.14% ± 6.39 at 400 mg/kg and 89.75% ± 3.44 for diclofenac (1%). When applied locally, the 10% cream has an inflammation inhibition of 96.87% ± 5.85 and 76.14% ± 7.88 for the 5% cream with 89.87 ± 6.20 of reference (Indomethacin). The abdominal contractions of rats treated with the root extract are significantly lower than those of the control group that received only physiological NaCl solution, with 41.20 ± 2.30 for the extract and 82.20 ± 5.04 for NaCl and 53.40 ± 4.94 for the reference. The healing activity of the studied extract records a percentage of contraction of about 93.20% ± 3.36 (Extract 10%), 84.50% ± 3.84 (Extract 5%), 48.47% ± 2.15 (control) and 81.88 ± 2.24 for the reference.

LC-DAD-ESI-MS/MS analysis, cytotoxic and antiproliferative effect of chlorogenic acid derivates rich extract from Nerium oleander L. pink flowers

2021 ◽  
Author(s):  
Siham Ayouaz ◽  
Sheila Cristina Oliveira-Alves ◽  
Ana Teresa Serra ◽  
Khalef LEFSIH ◽  
Madani Samah ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Chlorogenic Acid ◽  
Antiproliferative Effect ◽  
Nerium Oleander ◽  
Phenolic Composition ◽  
Esi Ms ◽  
Ms Analysis

Nerium oleander L, is a medicinal plant widely used for pharmaceutical purposes. In this work the pink flowers of this plant were characterized in terms of phenolic composition by LC-DAD-ESI-MS/MS...

scholarly journals Increasing medicinal and phytochemical compounds of coneflower (Echinacea purpurea L.) as affected by NO3−/NH4+ ratio and perlite particle size in hydroponics

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Fatemeh Ahmadi ◽  
Abbas Samadi ◽  
Ebrahim Sepehr ◽  
Amir Rahimi ◽  
Sergey Shabala
Keyword(s):  
Particle Size ◽  
Caffeic Acid ◽  
Culture Media ◽  
Chemical Compounds ◽  
Echinacea Purpurea ◽  
Hydroponic Culture ◽  
Peat Moss ◽  
Root Extract ◽  
Caffeic Acid Derivatives ◽  
Phytochemical Compounds

AbstractMedicinal plants are considered as one of the most important sources of chemical compounds, so preparing a suitable culture media for medicinal plant growth is a critical factor. The present study is aimed to improve the caffeic acid derivatives and alkylamides percentages of Echinacea purpurea root extract in hydroponic culture media with different perlite particle size and NO3−/NH4+ ratios. Perlite particle size in the growing media was varied as very coarse perlite (more than 2 mm), coarse perlite (1.5–2 mm), medium perlite (1–1.5 mm), fine perlite (0.5–1 mm), and very fine perlite (less than 0.5 mm) in different ratios to peat moss (including pure perlite, 50:50 v/v, 30:70 v/v, and pure peat moss). Two NO3−/NH4+ ratios (90:10 and 70:30) were tested in each growing media. All phytochemical analyses were performed according to standard methods using high performance liquid chromatography (HPLC). It was found that the E. purpurea grown in the medium containing very fine-grade perlite with 50:50 v/v perlite to peat moss ratio had the maximum caffeic acid derivatives, including chicoric acid (17 mg g−1 DW), caftaric acid (6.3 mg g−1 DW), chlorogenic acid (0.93 mg g−1 DW), cynarin (0.84 mg g−1 DW), and echinacoside (0.73 mg g−1 DW), as well as, alkylamides (54.21%). The percentages of these phytochemical compounds increased by decreasing perlite particle size and increasing of NO3−/NH4+ ratio. The major alkylamide in the E. purpurea root extract was dodeca-2E, 4E, 8Z-10 (E/Z)-tetraenoic acid isobutylamide in all treatments, ranging from 31.12 to 54.21% of total dry weight. It can be concluded that optimizing hydroponic culture media and nutrient solution has significant effects on E. purpurea chemical compounds.

NMR, HPLC-ESI-MS, and MALDI-TOF MS Analysis of Condensed Tannins from Delonix regia (Bojer ex Hook.) Raf. and Their Bioactivities

2012 ◽  
Vol 60 (19) ◽  
pp. 5013-5022 ◽  
Author(s):  
Wei-Ming Chai ◽  
Yan Shi ◽  
Hui-Ling Feng ◽  
Ling Qiu ◽  
Hai-Chao Zhou ◽  
...  
Keyword(s):  
Condensed Tannins ◽  
Maldi Tof Ms ◽  
Esi Ms ◽  
Delonix Regia ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Tof Ms
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