scholarly journals MORPHOMETRIC AND HISTOCHEMICAL CHARACTERISTICS OF THE CRIBRIFORM TYPE OF INTRADUCTAL CARCINOMA OF THE MAMMARY GLAND

2019 ◽  
Vol 72 (5) ◽  
pp. 748-752
Author(s):  
Dmytro Y. Nikolenko ◽  
Dmytro M. Boiko ◽  
Olexandr A. Shkurupii ◽  
Oksana V. Ovcharenko
Keyword(s):  
Mammary Gland ◽  
Periodic Acid ◽  
Protein Structures ◽  
Basal Membrane ◽  
Intraductal Carcinoma ◽  
Complex Method ◽  
Biopsy Material ◽  
Periodic Acid Schiff ◽  
Atypical Cells ◽  
Rhythmic Growth

Introduction: Due to the increasing morbidity and mortality rates from breast cancer, the problem of early, especially morphological diagnosis, continues to be important. The aim of this study to investigate the karyometric and histochemical features of cribriform pattern of parenchyma of intraductal carcinoma of the mammary gland. Materials and methods: Operational and biopsy material was studied in form of serial sections of micropreparations of cribriform type intraductal carcinoma of the mammary gland. Fixation with 10% neutral formalin, paraffin sections are stained with hematoxylin and eosin, complex Bergman + Periodic Acid - Schiff (PAS) reaction + alcyan blue. Cariometry was performed on 100 parenchymal tumor cell, tenth logarithms of the nucleus volume (LgV) were determined, kariocavirogram was constructed. Results: The atypical cellular polymorphism of the tumor, cribriform structures with cancerous cells in the state of apoptosis was revealed. The modal nuclear classes are defined in the interval LgV 0,75; 0,9; 1,2; 1,45, and do not comply with the law of rhythmic growth of nuclei in normal and confirm the cellular atypism of a cancerous tumor. Results are deprived of subjectivity through mathematical analysis of data in percentages (%). Histochemically, in the cytoplasm of cancer cells, precursors of lipids were detected in the form of a Schiff (PAS) positive reaction; alcyan-positive basal membrane of the duct; Bergman-positive protein structures in the nuclei. Conclusions: Cariometry and histochemical analysis of intraductal carcinoma of the mammary gland is an objective and accessible complex method for the analysis of atypical cells, reveals the degree of differentiation, apoptosis of cells and non-invasive stage of cancer.

scholarly journals GAMBARAN MIKROSKOPIK GINJAL TIKUS WISTAR (RATTUS NORVEGICUS) SETELAH DIINDUKSI DENGAN GENTAMISIN

2013 ◽  
Vol 4 (3) ◽  
Author(s):  
Poppy M Lintong ◽  
Carla F Kairupan ◽  
Priska L N Sondakh
Keyword(s):  
Body Weight ◽  
Epithelial Cells ◽  
Wistar Rats ◽  
Periodic Acid ◽  
Basal Membrane ◽  
Tubular Epithelial Cells ◽  
Periodic Acid Schiff ◽  
Group Iii ◽  
Group I ◽  
Group Ii

Abstract: Gentamycin, a frequently used aminoglycoside antibiotics, has a nephrotoxic effect to human beings and animals. The purpose of this research was to find out the microscopic changes of wistar rat kidneys after gentamycin induction. This was an experimental study, using five adult wistar rats, divided into three groups. Group I was the control group; group II consisted of two rats, injected with gentamycin 0,3 ml/day (dose of 60 mg/kg body weight/day) intraperitoneally for seven days; and group III consisted of two rats, injected with gentamycin 0,3 ml/day intraperitoneally for 10 days. Group I and II were terminated at day-8, and group III at day-11. Their kidneys were processed for microscopic slides, stained with hematoxylin eosin and Periodic Acid Schiff. In microscopic evaluation, group II and III showed oedema, necrosis, apoptosis, and basal membrane destruction of tubular epithelial cells. Group III also showed fat vacuoles in these epithelial cells (macrovesicular fatty changes). Conclusion: wistar rats injected with gentamycin 60 mg/kg body weight/day for 7 and 10 days showed oedema, necrosis, apoptosis, and basal membrane destruction of tubular epithelial cells; and macrovesicular fatty changes after 10 days of gentamycin.Key words: gentamycin, necrosis tubular epithelial cells, fatty changesAbstrak: Gentamisin termasuk antibiotik golongan aminoglikosida berspektrum luas yang bersifat nefrotoksik terhadap manusia dan hewan. Tujuan penelitian ini untuk melihat perubahan mikroskopik struktur ginjal tikus Wistar setelah diberikan gentamisin. Metode penelitian eksperimental dengan menggunakan lima ekor tikus Wistar dewasa yang dibagi atas tiga kelompok. Kelompok I tanpa perlakuan; kelompok II terdiri dari dua ekor tikus perlakuan yang diinjeksi dengan gentamisin 0,3 ml/hari (dosis 60 mg/kgBB/hari) secara intraperitonial selama tujuh hari; dan kelompok III terdiri dari dua ekor tikus perlakuan yang diinjeksi dengan gentamisin 0,3 ml/hari secara intraperitonial selama 10 hari. Tikus Wistar kelompok I dan II diteminasi hari ke-8, sedangkan kelompok III diterminasi hari ke-11. Ginjal tikus kelompok I -III kemudian dibuat preparat histopatologik dengan pengecatan rutin hematoksilin eosin dan Periodic Acid Schiff (PAS). Hasil penelitian menunjukkan tikus Wistar perlakuan yang diberikan gentamisin 0,3 ml/hari selama 7 sampai 10 hari secara mikroskopik memperlihatkan pembengkakan, nekrosis, apoptosis, dan destruksi membrana basalis sel epitel tubulus; dan pada hari ke-10 terlihat vakuol-vakuol lemak pada sel epitel sehingga inti terdesak ke tepi (perlemakan makrovesikuler). Simpulan: pemberian gentamisin pada tikus Wistar dengan dosis 60 mg/kg BB/hari selama 7-10 hari menunjukkan pembengkakan, nekrosis, apoptosis sel epitel tubulus, dan membrana basalis tubulus rusak; dan setelah hari ke-10 juga terlihat perlemakan makrovesikuler.Kata kunci: gentamisin, nekrosis sel epitel tubulus, perlemakan makrovesikuler

scholarly journals Histologic and inflammatory lamellar changes in horses with oligofructose-induced laminitis treated with a CXCR1/2 antagonist

2016 ◽  
Vol 36 (1) ◽  
pp. 13-18 ◽  
Author(s):  
Leonardo R. de Lima ◽  
Heloisa M.F. Mendes ◽  
Frederico M. Soriani ◽  
Danielle G. de Souza ◽  
Geraldo Eleno S. Alves ◽  
...  
Keyword(s):  
Chemokine Receptors ◽  
Periodic Acid ◽  
Previous History ◽  
Basal Membrane ◽  
Treated Group ◽  
Periodic Acid Schiff ◽  
Chemokine Signaling ◽  
Mixed Breed ◽  
History Of ◽  
Membrane Changes

Abstract: With the hypothesis that blocking chemokine signaling can ameliorate acute laminitis, the aim was to evaluate the therapeutic effect of intravenous DF1681B, a selective antagonist for CXCR1 and CXCR2 (chemokine receptors), in an oligofructose equine laminitis model. To twelve mixed breed clinically healthy hoses with no previous history of hoof-related lameness was administered oligofructose (10g/kg given by nasogastric tube) and divided into two groups: treated (intravenous DF1681B at 30mg/kg 6, 12, 18, and 24h after oligofructose) and non-treated groups. Laminar biopsies were performed before and 12, 36, and 72h after administering oligofructose. Samples were stained with periodic acid-Schiff (PAS) and scored from 0 to 6 according to epidermal cell and basal membrane changes. The IL-1β, IL-6, and CXCL1 RNA expressions were determined by RT-PCR. Parametric and non-parametric tests were used to compare times within each group (P<0.05). The PAS grades and IL-1β and IL-6 RNA expression increased in the non-treated group, but remained constant in the treated horses. In conclusion, DF1681B therapy reduced laminar inflammation and epidermal deterioration in treated horses. CXCR1/2 blockage should be considered therapeutically for equine acute laminitis.

scholarly journals Relationship between the morphologic alterations of vocal cords from adult autopsies and the cause of death

2004 ◽  
Vol 59 (2) ◽  
pp. 63-66 ◽  
Author(s):  
Ana Karina Marques Salge ◽  
Eumenia Costa da Cunha Castro ◽  
Mara Lúcia Fonseca Ferraz ◽  
Marlene Antônia dos Reis ◽  
Vicente de Paula Antunes Teixeira
Keyword(s):  
Vocal Cord ◽  
Cause Of Death ◽  
Respiratory Diseases ◽  
Periodic Acid ◽  
Basal Membrane ◽  
Vocal Cords ◽  
Periodic Acid Schiff ◽  
White Ethnicity ◽  
Staining Methods ◽  
Hematoxylin Eosin

PURPOSE: The purpose of this study was to identify the possible alteration in the thickness of the epithelium basal membrane of the vocal cords and correlate it with the cause of death. METHOD: Larynxes collected from adult autopsies during the period of 1993 to 2001 were utilized. We used the hematoxylin-eosin and periodic acid-Schiff staining methods for the morphological and morphometric analysis. RESULTS: Sixty-six vocal cords were analysed; increased thickness was identified in 14 cases (21.2%), with equal proportions between the genders. Increased vocal-cord thickness was more frequent in patients of the white ethnicity (12 cases, 85.7%). Respiratory alterations were found in 10 (71.4%) of the cases with increased vocal-cord thickness. Of the patients that were maintained with mechanical ventilation before death, 7 (18.4%) had thickening of the basal membrane. Among the smokers, 9 (19.63%) had basal membrane thickening. CONCLUSION: No statistically significant differences were found between the cases in which the cause of death was related to respiratory diseases as compared to non-respiratory diseases and the thickening of the basal membrane of the vocal cords. However, new studies are needed in order to verify the etiopathogenesis of this thickening.

scholarly journals Actinomycotic Endomyometritis Associated with a Long-Term Use of Intrauterine Device Lasting for 42 Years

2019 ◽  
Vol 62 (1) ◽  
pp. 35-38
Author(s):  
Vladimír Bartoš ◽  
Jana Doboszová ◽  
Martin Sudek
Keyword(s):  
Intrauterine Device ◽  
Periodic Acid ◽  
Total Abdominal Hysterectomy ◽  
Abdominal Hysterectomy ◽  
Microscopic Investigation ◽  
Silver Stain ◽  
Biopsy Material ◽  
Periodic Acid Schiff ◽  
Large Round ◽  
Methenamine Silver Stain

In women, pelvic actinomycosis is closely associated with prolonged use of the intrauterine devices (IUD). A 70-year old female presented with intermittent blood-stained vaginal discharge. An analysis of her history revealed, she was inserted with an IUD 42 years ago, but it has remained in situ untill now. Curettage of the uterus was done, but an IUD was firmly attached inside the cavity and there was not able to remove it. A biopsy material consisted of the large round and oval granules of filamentous and mycelium-like microorganisms. They showed strong positivity with Periodic acid–Schiff stain and Gömöri methenamine silver stain. Histopathology was consisted with uterine actinomycosis. A total abdominal hysterectomy with bilateral adnexectomy was performed. The uterus contained a retained plastic IUD. Microscopic investigation revealed a diffuse chronic active endomyometritis with sporadic Actinomycetes colonies. Wearing an IUD continuously for very long periods of time can lead to actinomycotic infection, which may manifest for many years after its application. All IUD users have to keep in mind regular gynecological check-ups to avoid the complications of a retained and “forgotten” IUD.

scholarly journals Pericardial mesothelioma in a German Shepherd dog: a case report

2013 ◽  
Vol 58 (No. 11) ◽  
pp. 594-598 ◽  
Author(s):  
S. Ceribasi ◽  
M. Ozkaraca ◽  
AO Ceribasi ◽  
H. Ozer
Keyword(s):  
Case Report ◽  
Periodic Acid ◽  
Cell Formation ◽  
Basal Membrane ◽  
German Shepherd ◽  
Neoplastic Cells ◽  
Periodic Acid Schiff ◽  
Yellow Colour ◽  
German Shepherd Dog ◽  
Pericardial Mesothelioma

In this case report, a diagnosis of pericardial mesothelioma in a four year old male German shepherd dog is described. The dog, which had anorexia, bloody diarrhoea, dehydration and depression and, died on day&nbsp;10 of therapy, was systematically necropsied. At necropsy, approximately 1.5 litres of cloudy and bloody exudate were detected in the thoracic cavity. The parietal lamina of the pericardium was covered with multilobular nodular masses related with each other, 1 to 5 cm in diameter and grey-yellow in colour. There were proliferations characterised with grey-yellow colour and approximately 1 to 5 mm in length on visceral pleura. The presence of abscess foci with liquefied-centrum was observed when examining a section surface of the lung. The appearance of the oesophageal serosa, thoracic aorta and the thoracic section of the diaphragm were similar to pleura. A thickening was microscopically determined in the parietal lamina of the pleura and pericardium due to papillary proliferations consisting of cells similar to cubic or cylindrical epithelium. Severe lymphocyte and plasma cell infiltrations were observed in the pleural sections next to the lung. Neoplastic cells had nuclei with large eosinophilic granular cytoplasms and large vesicular and single nucleoli. Some neoplastic cells were determined to include intracytoplasmic vacuoles. The neoplastic cells contained some mitotic figures. It was observed that some tumour cells contributed to giant cell formation through integration. In periodic acid Schiff-haematoxylin (PAS-H) examinations it was determined that the pleural basal membrane maintained its integrity. Immunohistochemically, the tumour gave a weak positive reaction with anti-pancytokeratin staining while giving intense reaction with anti-vimentin staining.

scholarly journals CHARACTERISTIC OF SKIN MORPHOLOGY OF SUNDA PORCUPINE (Hystrix javanica) WITH SPECIAL REFERENCE TO THE CONNECTIVE TISSUE

2018 ◽  
Vol 12 (1) ◽  
Author(s):  
Andhika Yudha Prawira ◽  
Desrayni Hanadhita ◽  
Anisa Rahma ◽  
Supratikno Supratikno ◽  
Savitri Novelina ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Periodic Acid ◽  
Basal Membrane ◽  
Wide Distribution ◽  
Periodic Acid Schiff ◽  
Characteristic Type ◽  
Elastin Fiber ◽  
Staining Methods ◽  
Skin Morphology ◽  
Dominant Structure

This study was conducted to investigate the histological characteristic, type, and distribution of connective tissue in Sunda porcupine skin. The investigation was carried out in three adult of sunda porcupines at microscopic level using hematoxylin eosin, Masson thrichrome, Verhoeffs van Gieson, alcian blue pH 2.5 and periodic acid Schiff staining methods. Skin consists of epidermis, dermis hypodermis, and subcutaneous muscle. Quill follicles were the main and dominant structure as well as the specific characteristic on Sunda porcupine skin. The connective tissue was distributed well in basal membrane, dermis, quill follicle, and hypodermis with various intensity and density. The collagen was the main fiber found in the skin while the elastin fiber was not observed. The acid carbohydrate was found distributed well in the skin while the neutral carbohydrate was not detected in this study. In addition the fibers of connective tissue associated with the adipose tissue which found plentifully in quill follicles and hypodermis. The present results showed that the wide distribution of connective tissue might have an important role on the wound healing physiology of Sunda porcupine skin.

scholarly journals Lipid-rich Carcinoma of the Mammary Gland in a Cat

2005 ◽  
Vol 42 (3) ◽  
pp. 360-362 ◽  
Author(s):  
D. A. Kamstock ◽  
R. Fredrickson ◽  
E. J. Ehrhart
Keyword(s):  
Mammary Gland ◽  
Alcian Blue ◽  
Microscopic Examination ◽  
Mammary Carcinoma ◽  
Periodic Acid ◽  
Surgical Excision ◽  
Mammary Tissue ◽  
Periodic Acid Schiff ◽  
First Report ◽  
Subcutaneous Mass

A 1.5-year-old female, intact, clinically healthy cat presented for a subcutaneous mass of the ventral abdomen. surgical excision and microscopic examination of the mass were performed. Histologically, this was a discrete, unencapsulated, multilobular, expansile mass, which compressed the surrounding normal mammary tissue. Lobules were composed of tubuloacinar structures formed by atypical round to polygonal cells, which contained foamy to microvacuolated cytoplasm and variably sized, intracytoplasmic, distinct vacuoles causing nuclear peripheralization. Neoplastic cells demonstrated intense and diffuse immunoreactivity for cytokeratin and lacked immunoreactivity for vimentin. The vacuolar contents stained positively with oil RedO and negatively with periodic acid-Schiff and Alcian blue stains. Histomorphologic, histochemical, and immunohistochemial analysis support a diagnosis of lipid-rich mammary carcinoma. This is the first report of a cat with a lipid-rich variant of mammary carcinoma.

DISAPPEARANCE OF HISTOCHEMICALLY-DEMONSTRABLE ADENOSINE TRIPHOSPHATASE AND OF PAS-REACTIVE BASEMENT MEMBRANES IN BLOOD VESSELS IN THE DECIDUA DURING OVUM IMPLANTATION IN MICE

1968 ◽  
Vol 41 (1) ◽  
pp. 53-59 ◽  
Author(s):  
KATHLEEN HALL
Keyword(s):  
Atpase Activity ◽  
Blood Vessels ◽  
Adenosine Triphosphatase ◽  
Plasma Membranes ◽  
Periodic Acid ◽  
Basal Membrane ◽  
Close Correlation ◽  
Basement Membranes ◽  
Periodic Acid Schiff ◽  
Decidual Cells

SUMMARY Histochemical studies were made of the endometrial blood vessels before and after normal implantation of blastocysts in intact mice and delayed implantation in pregnant, spayed, hormone-treated mice. Adenosine triphosphatase (ATPase) activity was investigated by the calcium method of Padykula and Herman and the lead method of Wachstein and Meisel, controlled by inclusion of inhibitors and by substitution of other substrates. Paraffin sections were subjected to the periodic acid-Schiff (PAS) technique. In the endothelium of endometrial blood vessels intense ATPase staining occurred on days 2 to 5 in intact mice and until at least day 9 in spayed mice injected with progesterone alone (no implantation), and in the non-decidual areas after normal (day 6) or induced (days 7–9) implantation. The reaction appeared to be localized in the endothelial plasma membranes, mainly in the basal membrane. The basement membranes of these vessels reacted strongly with PAS. Within decidual tissue at the implantation sites in both intact and spayed mice ATPase activity was completely absent from all blood vessels. The basement membranes of these vessels gave no reaction with PAS. There was close correlation in distribution and time of appearance between absence of ATPase activity and differentiation of alkaline phosphatase-reactive decidual cells. The specificity of the vascular reaction with ATP is considered in the light of its behaviour towards enzyme inhibitors and other substrates, and the significance of its disappearance in decidual blood vessels is discussed.

Sign in / Sign up

Export Citation Format

Share Document