Bactericidal properties of a combined corrosion inhibitor in the presence of SRB

2020 ◽  
Vol 25 (3) ◽  
Keyword(s):  
Hydrogen Sulfide ◽  
Hydrogen Diffusion ◽  
Desulfovibrio Desulfuricans ◽  
Bacterial Cells ◽  
Bacteriostatic Effect ◽  
Sulfate Reducing ◽  
Bactericidal Properties ◽  
Number Of Microorganisms ◽  
Reducing Bacteria ◽  
Steel St3

The bactericidal properties of the combined inhibitor with respect to two types of Desulfovibrio desulfuricans and Desulfomicrobium sulfate-reducing bacteria were studied. The effect of the inhibitor on the number of bacterial cells and the formation of hydrogen sulfide in Postgate nutrient Postgate “B” was evaluated. It has been shown that the combined inhibitor exhibits a bacteriostatic effect on to sulphate-reducing bacteria. It was revealed that the degree of suppression of the number of microorganisms Desulfovibrio desulfuricans at a concentration of the combined inhibitor 100,0 mg/L is higher than Desulfomicrobium. In the latter case, to achieve this effect, 120,0 mg/L concentration of the combined inhibitor is required. The studied combination inhibitor causes the inhibition of hydrogen diffusion in steel St3 in the MI medium saturated with H2S and CO2 separately and together, and also contributes to preserving the ductile properties of the steel St3 after exposure to solutions compared to non-inhibited media.

scholarly journals STUDY OF UNIVERSAL COMBINED INHIBITOR FOR OIL AND GAS INDUSTRY

2020 ◽  
Vol 63 (10) ◽  
pp. 78-89
Author(s):  
Guseyn R. Gurbanov ◽  
Saida M. Pashaeva ◽  
Mekhpara B. Adygezalova
Keyword(s):  
Carbon Dioxide ◽  
Hydrogen Sulfide ◽  
Corrosion Rate ◽  
Oil And Gas ◽  
Hydrogen Diffusion ◽  
Gravimetric Method ◽  
Oil And Gas Industry ◽  
Bacterial Cells ◽  
Reducing Bacteria ◽  
Steel St3

Using the gravimetric method, the inhibitory efficiency of the combined inhibitor with respect to hydrogen sulfide and carbon dioxide corrosion of St3 steel in model-produced water MI was studied. Corrosion tests were carried out in 0.5 l sealed vessels on St3 samples of size 30×20×1. Gossypol resin + MARZA was used as a multifunctional combined inhibitor. Diesel fuel and kerosene were used as solvent. It has been established that the protective effect of using a multifunctional combined inhibitor in formation water with oil containing hydrogen sulphide and carbon dioxide using kerosene as a solvent ranges from 75 to 96 and for diesel as 80 to 100. The combined inhibitor allows to achieve in the MI medium containing hydrogen sulfide and carbon dioxide in the process of daily testing the corrosion rate of steel is about 0.04 g/ m2∙h only in a concentration of not less than 70 mg/l. However, with an increase in the duration of the test by an order of magnitude, a similar corrosion rate is observed already at an inhibitor concentration of 50 mg/l. The same is characteristic of carbon dioxide and hydrogen sulfide - carbon dioxide solutions. The bactericidal properties of the combined inhibitor with respect to two types of Desulfovibriodesulfuricans and Desulfomicrobium sulfate-reducing bacteria were studied. The effect of the inhibitor on the number of bacterial cells and the formation of hydrogen sulfide in Postgate nutrient medium “B” was evaluated. It has been shown that the combined inhibitor exhibits a bacteriostatic effect on to sulphate-reducing bacteria. It was revealed that the degree of suppression of the number of microorganisms Desulfovibriodesulfuricans at a concentration of the combined inhibitor 100.0 mg/l is higher than Desulfomicrobium. In the latter case, to achieve this effect, 120.0 mg/l concentration of the combined inhibitor is required. The studied combination inhibitor causes inhibition of hydrogen diffusion in steel St3 in the MI medium saturated with H2S and CO2 separately and together, and contributes to preserving the ductile properties of the steel St3 after exposure to solutions compared to non-inhibited media.

scholarly journals Universalism of inhibitors against hydrogen sulfide and carbon dioxide corrosion of carbon steel

2021 ◽  
Vol 225 ◽  
pp. 05001
Author(s):  
Vladimir Vigdorovich ◽  
Liudmila Tsygankova ◽  
Natalia Shel ◽  
Nedal Alshikha
Keyword(s):  
Carbon Dioxide ◽  
Hydrogen Sulfide ◽  
Carbon Steel ◽  
Hydrogen Diffusion ◽  
Electrochemical Impedance ◽  
Negative Impact ◽  
Sulfate Reducing Bacteria ◽  
Carbon Dioxide Corrosion ◽  
Sulfate Reducing ◽  
Reducing Bacteria

The universality of inhibitors is understood as their ability to inhibit several types of corrosion attack: hydrogen sulfide and carbon dioxide corrosion, hydrogen diffusion into metal, development of sulfate-reducing and other types of bacteria, negative impact on the mechanical properties of metals. Indicators of universalism of new inhibitor have been studied. Producer of the inhibiting compositions is Limited Liability Company «INCORGAZ» (S-Petersburg, Russia). The efficacy of the inhibitor in the concentration of 25 - 200 mg/L has been studied with respect to carbon steel in a highly mineralized chloride medium (pH= 6) and NACE medium (5 g/L NaCl, 0.25 g/L CH3COOH, pH =3.5) containing H2S (50-400 mg/L) and CO2 (1at) separately and together. The bactericidal properties of the inhibitor were studied with respect to sulfate-reducing bacteria in the Postgate medium. The investigations were carried out by the methods of linear polarization resistance, electrochemical impedance spectroscopy, gravimetry, potentiodynamic polarization. The protective effectiveness of the inhibitor reaches 80% in the presence of CO2 and 90% in hydrogen sulphide environments. The inhibitor repeatedly reduces the number of sulfate-reducing bacteria and the production of biogenic hydrogen sulfide and inhibits the diffusion of hydrogen into steel.

Study of bactericide properties of MARZA-1 agent in laboratory conditions

2021 ◽  
pp. 33-38
Author(s):  
H.R. Gurbanov ◽  
◽  
M.B. Adygozelova ◽  
S.F. Ahmadov ◽  
S.M. Pashayeva ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Desulfovibrio Desulfuricans ◽  
Sulfate Reducing Bacteria ◽  
Cell Disruption ◽  
Sulfate Reducing ◽  
Laboratory Conditions ◽  
Comparison Of Results ◽  
Nutritional Medium ◽  
Reducing Bacteria

Bactericide properties of MARZA-1 inhibitor were studied with regard to desulfovibrio-desulfuricans and desulfo-microbium sulfate-reducing bacteria. According to the results of experiments, the effect of MARZA-1 inhibitor on the quantity of sulfate-reducing bacteria cells and formation of biogenic hydrogen-sulfide in Postgate-B nutritional medium has been estimated. MARZA-1 agent showed high bactericide effect towards desulfovibrio-desulfuricans and sulfate-reducing bacteria of desulfo-microbium type. The comparison of results with both bacteria justified that the rate of SRB cell disruption in the medium with desulfovibrio-desulfurican bacteria in the concentration of 7 mg/l on the fifth-seventh days, and in desulfo-microbium bacteria is observed with 10 mg/l concentration of MARZA-1 agent on the seventh day.

scholarly journals Dissimilatory sulfate reduction in bacteria Desulfovibrio desulfuricans ІМV К-6 upon influence of Uragan and Raundup herbicides

2015 ◽  
Vol 6 (1) ◽  
pp. 40-44
Author(s):  
G. І. Zvir ◽  
O. М. Moroz ◽  
S. O. Hnatush
Keyword(s):  
Hydrogen Sulfide ◽  
Sulfate Reduction ◽  
Colorimetric Method ◽  
Reduction Process ◽  
Desulfovibrio Desulfuricans ◽  
Sulfate Reducing Bacteria ◽  
Dissimilatory Sulfate Reduction ◽  
Sulfate Ions ◽  
Sulfate Reducing ◽  
Reducing Bacteria

Objects of the study were sulfate-reducing bacteria Desulfovibrio desulfuricans ІМV К-6, isolated from Yavorivske lakе. This strain is kept in the collection of microorganisms at the Department of Microbiology of Ivan Franko National University. Bacteria were grown in the Kravtsov-Sorokin’s liquid medium with the following composition (g/l): Na2SO4 × 10H2O – 0.5, NaH2PO4 – 0.3, K2HPO4 – 0.5, (NH4)2SO4 – 0.2, MgSO4 × 7H2O – 0.1, C3H5O3Na – 2.0. The bacteria were grown for 10 days at 30 °C under anaerobic conditions. In order to study the sensitivity of the sulfate reducing bacteria to action of Uragan and Raundup herbicides, the cells of D. desulfuricans ІМV К-6 were grown at the concentrations of herbicides as follows: 0,28 mМ, 2,8 mМ (concentration recommended for use) and 5,6 mM. Biomass was determined by photometric method. Concentration of hydrogen sulfide in the culture medium was determined by photo-colorimetric method. Concentration of sulfate-ions in the medium was determined by turbidimetric method. Capacity of sulfate reducing bacteria D. desulfuricans ІМV K-6 to grow, reducing sulfates to hydrogen sulfide upon influence of Uragan and Raundup herbicides was studied. Accumulation of bacterial biomass in the control and upon influence of herbicides was the highest on the fourth-sixth day of cultivation, and after that the stationary growth phase began. It was shown that sulfate reducing bacteria upon influence of herbicides grew more intensively compared with the control. It was discovered that the level of biomass changed depending on the increasing concentration of Uragan or Raundup herbicides in the medium. Sulfate reducing bacteria D. desulfuricans ІМV K-6 could reduce sulfates to hydrogen sulfide in the presence of sulfates and organic compounds in the medium (dissimilatory sulfate reduction). Stimulatory influence of Uragan and Raundup on the dissimilatory sulfate reduction process of D. desulfuricans ІМВ К-6 has been discovered. The formation of hydrogen sulfide correlates with the usage of sulfatе ions. The capacity of sulfate reducing bacteria D. desulfuricans ІМV K-6 to grow, reducing sulfate ions to hydrogen sulfide upon influence of Uragan and Raundup may be caused by presence of inert components (sulfates) in these herbicides that can be used by microorganisms as electron acceptors during sulfate respiration. 

Fluorescence chemosensors for hydrogen sulfide detection in biological systems

The Analyst ◽  
2015 ◽  
Vol 140 (6) ◽  
pp. 1772-1786 ◽  
Author(s):  
Zhi Guo ◽  
Guiqiu Chen ◽  
Guangming Zeng ◽  
Zhongwu Li ◽  
Anwei Chen ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Biological Systems ◽  
Sulfate Reducing Bacteria ◽  
Fluorescence Sensing ◽  
Sulfate Reducing ◽  
Potential Applications ◽  
Reducing Bacteria

The development of H2S fluorescence-sensing strategies and their potential applications in the determination of sulfate-reducing bacteria activity.

Suppressing sulfate-reducing bacteria, as well as hydrogen diffusion through steel, by inhibitors of AMDOR IC type

2007 ◽  
Vol 43 (1) ◽  
pp. 95-99
Author(s):  
V. I. Vigdorovich ◽  
M. V. Vigdorovich ◽  
A. V. Ryazanov ◽  
A. N. Zavershinskii
Keyword(s):  
Hydrogen Diffusion ◽  
Sulfate Reducing Bacteria ◽  
Sulfate Reducing ◽  
Reducing Bacteria

Experimental Investigation on the Active Range of Sulfate-Reducing Bacteria for Geological Disposal

1994 ◽  
Vol 353 ◽  
Author(s):  
S. Fukunaga ◽  
H. Yoshikawa ◽  
K. Fujiki ◽  
H. Asano
Keyword(s):  
Experimental Investigation ◽  
Environmental Conditions ◽  
Desulfovibrio Desulfuricans ◽  
Sulfate Reducing Bacteria ◽  
Geological Disposal ◽  
Sulfate Reducing ◽  
Buffer Material ◽  
Ph Range ◽  
High Level ◽  
Reducing Bacteria

AbstractThe active range ofDesulfovibrio desulfuricans. a species of sulfate-reducing bacteria, was examined in terms of pH and Eh using a fermenter at controlled pH and Eh. Such research is important because sulfate-reducing bacteria (SRB) are thought to exist underground at depths equal to those of supposed repositories for high-level radioactive wastes and to be capable of inducing corrosion of the metals used in containment vessels.SRB activity was estimated at 35°C, with lactate as an electron donor, at a pH range from 7 to 11 and Eh range from 0 to -380 mV. Activity increased as pH approached neutral and Eh declined. The upper pH limit for activity was between 9.9 and 10.3, at Eh of -360 to -384 mV. The upper Eh limit for activity was between -68 and -3 mV, at pH 7.1. These results show that SRB can be made active at higher pH by decreasing Eh, and that the higher pH levels of 8 to 10 produced by use of the buffer material bentonite does not suppress SRB completely.A chart was obtained showing the active range ofDesulfovibrio desulfuricansin terms of pH and Eh. Such charts can be used to estimate the viability of SRB and other microorganisms when the environmental conditions of a repository are specified.

scholarly journals APPLICATION OF NATURAL PETROLEUM ACIDS AS HEXAMETYLENDIAMIN COMPLEX COMBINES AS BACTERIA

2021 ◽  
Vol 10 (06) ◽  
pp. 58-63
Author(s):  
Teyyub İsmayılov, Sevinj Suleymanova Teyyub İsmayılov, Sevinj Suleymanova
Keyword(s):  
Room Temperature ◽  
Bactericidal Effect ◽  
Complex Compounds ◽  
Chemical Parameters ◽  
Sulfate Reducing ◽  
Bactericidal Properties ◽  
Physical And Chemical ◽  
Reducing Bacteria ◽  
Growth Of Bacteria ◽  
Petroleum Acid

Complex compounds were synthesized by mixing natural petroleum acids with hexamethylene diamine at a ratio of 1:1 and 2:1 at room temperature, IR spectra of the complexes were studied and confirmed, physical and chemical parameters were determined, solutions were prepared and bactericidal properties were studied. The bactericidal effect of a complex compound of natural petroleum acids synthesized with hexamethylenediamine in a ratio of 1: 1 mol at a concentration of 250 mg / l is 95%, a complex combination obtained at a concentration of 500 mg / l and 1000 mg / l is 100%, bactericidal at a concentration of 2: 1 mol at a concentration of 250 mg / l The effect was 98%, 100% at 500 and 1000 mg / l concentrations. According to the results, complex compounds of natural petroleum acids synthesized with hexamethylenediamine can be used to prevent the growth of bacteria. Keywords: natural petroleum acid, hexamethylenediamine, sulfate-reducing bacteria, complex compounds, bactericidal properties.

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