scholarly journals IDENTIFIKASI BAKTERI RESISTEN MERKURI PADA INDIVIDU DI DAERAH PESISIR PANTAI DI DESA BUDO KECAMATAN WORI

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Jefry Hamonangan Hinonaung
Keyword(s):  
Room Temperature ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Sulfate Reducing ◽  
Liquid Element ◽  
Bottom Waters ◽  
Biochemical Testing ◽  
Biology Laboratory ◽  
Reducing Bacteria ◽  
Mercury Resistant Bacteria

Abstract: Mercury is a silver liquid element at room temperature. Mercury form a variety of both inorganic and organic compounds. Mercury that goes into the sea, there is evaporated back into the atmosphere and fall to the ground experiencing methylation. Mercury in water can undergo methylation area with the help of the sulfate reducing bacteria and iron. Not only mercury from only rainwater but sediment mercury in the bottom waters can also be converted into methyl mercury. MeHg is harmful to humans, because it will accumulate MeHg in plankton or microorganisms. Then the plankton and microorganisms will be eaten by predators higher up the food chain in consumption by humans. This tudy aims to determine determine mercury resistant bacteria found in individuals in the coastal areas. The study design was a descriptive exploratory method. Samples taken in this study was a colony of mercury -resistant bacteria in tartar, urine and feces. Specimens were obtained put in a sterile pot and immediately brought to the biology laboratory in the MIPA Unsrat Manado Faculty to the identification of mercury -resistant bacteria and test. Isolation of mercury resistant bacterial isolates in 3 samples, obtained 6 isolates. Then test to identify bacteria with morphological, physiological testing, and biochemical testing. Results of a study found four genus of bacteria. Keywords: Mercury , Mercury Resistant Bacteria , Tartar , Urine , Feces     Abstrak:Merkuri merupakan suatu unsur berbentuk cair keperakan pada suhukamar. Merkuri membentuk berbagai persenyawaan baik anorganikmaupun organik. Merkuri yang masuk ke dalam laut, ada yang menguap kembali ke atmosfir dan jatuh ke tanah mengalami metilisasi. Merkuri dalam daerah perairan dapat mengalami metilisasi dengan bantuan bakteri pereduksi sulfat dan besi. Tidak hanya merkuri dari air hujan saja tetapi sedimen merkuri di dasar perairan juga dapat diubah menjadi metil merkuri. MeHg ini berbahaya bagi manusia, karena MeHg ini akan terakumulasi dalam plankton atau mikroorganisme. Kemudian plankton dan mikroorganisme ini akan di makan oleh predator yang lebih tinggi lagi dalam rantai makanan hingga di konsumsi oleh manusia. Penelitian ini bertujuan untuk mengetahui mengetahui bakteri resisten merkuri yang terdapat pada individu di daerah pesisir pantai. Desain penelitian adalah metode deskriptif eksploratif. Sampel yang diambil dalam penelitian ini adalah koloni bakteri resisten merkuri pada karang gigi, urin dan feses. Spesimen yang didapatkan dimasukkan ke dalam pot steril dan segera di bawa ke laboratoriumbiologi Fakultas MIPA Unsrat Manado untuk dilakukan identifikasi bakteri dan uji resisten merkuri. Isolasi isolat bakteri resisten merkuri pada 3 sampel, diperoleh 6 isolat. Kemudian dilakukan identifikasi bakteri dengan uji morfologi, uji fisiologi, dan uji biokimia. Hasil peneltian ditemukan 4 genus bakteri. Kata kunci: Merkuri, Bakteri Resisten Merkuri, Karang gigi, Urin, Feses

scholarly journals APPLICATION OF NATURAL PETROLEUM ACIDS AS HEXAMETYLENDIAMIN COMPLEX COMBINES AS BACTERIA

2021 ◽  
Vol 10 (06) ◽  
pp. 58-63
Author(s):  
Teyyub İsmayılov, Sevinj Suleymanova Teyyub İsmayılov, Sevinj Suleymanova
Keyword(s):  
Room Temperature ◽  
Bactericidal Effect ◽  
Complex Compounds ◽  
Chemical Parameters ◽  
Sulfate Reducing ◽  
Bactericidal Properties ◽  
Physical And Chemical ◽  
Reducing Bacteria ◽  
Growth Of Bacteria ◽  
Petroleum Acid

Complex compounds were synthesized by mixing natural petroleum acids with hexamethylene diamine at a ratio of 1:1 and 2:1 at room temperature, IR spectra of the complexes were studied and confirmed, physical and chemical parameters were determined, solutions were prepared and bactericidal properties were studied. The bactericidal effect of a complex compound of natural petroleum acids synthesized with hexamethylenediamine in a ratio of 1: 1 mol at a concentration of 250 mg / l is 95%, a complex combination obtained at a concentration of 500 mg / l and 1000 mg / l is 100%, bactericidal at a concentration of 2: 1 mol at a concentration of 250 mg / l The effect was 98%, 100% at 500 and 1000 mg / l concentrations. According to the results, complex compounds of natural petroleum acids synthesized with hexamethylenediamine can be used to prevent the growth of bacteria. Keywords: natural petroleum acid, hexamethylenediamine, sulfate-reducing bacteria, complex compounds, bactericidal properties.

Treatment of Acid Mine Drainage by Sulfate Reducing Bacteria and Iron at Room Temperature

2010 ◽  
Vol 113-116 ◽  
pp. 1500-1503
Author(s):  
Ying Feng ◽  
Yong Kang ◽  
Yan Fang Yu
Keyword(s):  
Room Temperature ◽  
Mine Drainage ◽  
Reduction Rate ◽  
Sulfate Reducing Bacteria ◽  
Treatment Efficiency ◽  
Sulfate Reducing ◽  
Acid Mine ◽  
Mine Wastewater ◽  
Reducing Bacteria ◽  
Acid Mining Drainage

This study describes a new method to treat acid mine wastewater containing high amounts of heavy metals and sulfate by biotechnology. Sulfate reducing Bacteria (SRB) was inoculated in an up-flow multiple bed bioreactor treating practical wastewater. In addition to precipitation processes, water purification was also possible with the metabolism process of microorganisms. Iron dust was added to the system to enhance the activity of SRB and ensure the treatment efficiency. The results indicates that treating acid mining drainage using SRB and iron at room temperature (20°C~25°C) is possible, the reduction rate of sulfate is up to 61%, pH of wastewater raises from 2.75 to 6.2 and the copper concentration of effluent is less than 0.2 mg/L.

Microbial diversity associated with the anaerobic sediments of a soda lake (Mono Lake, California, USA)

2018 ◽  
Vol 64 (6) ◽  
pp. 385-392 ◽  
Author(s):  
Patricia Rojas ◽  
Nuria Rodríguez ◽  
Vicenta de la Fuente ◽  
Daniel Sánchez-Mata ◽  
Ricardo Amils ◽  
...  
Keyword(s):  
Soda Lakes ◽  
Soda Lake ◽  
Mono Lake ◽  
Environmental Groups ◽  
Sulfate Reducing ◽  
Operational Taxonomic Units ◽  
Bottom Waters ◽  
Reducing Activity ◽  
High Sulfate ◽  
Reducing Bacteria

Soda lakes are inhabited by important haloalkaliphilic microbial communities that are well adapted to these extreme characteristics. The surface waters of the haloalkaline Mono Lake (California, USA) are alkaline but, in contrast to its bottom waters, do not present high salinity. We have studied the microbiota present in the shoreline sediments of Mono Lake using next-generation sequencing techniques. The statistical indexes showed that Bacteria had a higher richness, diversity, and evenness than Archaea. Seventeen phyla and 8 “candidate divisions” were identified among the Bacteria, with a predominance of the phyla Firmicutes, Proteobacteria, and Bacteroidetes. Among the Proteobacteria, there was a notable presence of Rhodoplanes and a high diversity of sulfate-reducing Deltaproteobacteria, in accordance with the high sulfate-reducing activity detected in soda lakes. Numerous families of bacterial fermenters were identified among the Firmicutes. The Bacteroides were represented by several environmental groups that have not yet been isolated. Since final organic matter in anaerobic environments with high sulfate contents is mineralized mainly by sulfate-reducing bacteria, very little methanogenic archaeal biodiversity was detected. Only 2 genera, Methanocalculus and Methanosarcina, were retrieved. The species similarities described indicate that a significant number of the operational taxonomic units identified may represent new species.

scholarly journals MERCURY REDUCTASE ACTIVITY OF AN INDIGENOUS MERCURY RESISTANT BACTERIAL ISOLATE (Bacillus sp. S1) FROM KALIMAS-SURABAYA AS A POTENTIAL REDUCING AGENT FOR MERCURIAL ION (HG2+)

2015 ◽  
Vol 2 (1) ◽  
pp. 446 ◽  
Author(s):  
Enny Zulaika ◽  
Langkah Sembiring
Keyword(s):  
Room Temperature ◽  
Bacterial Isolate ◽  
Reductase Activity ◽  
Elemental Mercury ◽  
Total Cell ◽  
Resistant Bacteria ◽  
Bacillus Sp ◽  
Reduction Efficiency ◽  
Mercury Reductase ◽  
Mercury Resistant Bacteria

<p>Mercury reductase activity and Hg2+ lowering capacity of a Mercury Resistant Bacteria (MRB) (Bacillus sp. S1) which was isolated from Kalimas River of Surabaya, Indonesia were studied. The activity was determined by Mercury Reductase Assay System (MRAS) in a solution mixture contained 50 mM PBS (pH ± 7.0), 0.5 mM EDTA, 200 µM MgSO4, 0.1% (v/v) ß-merchaptoethanol, 200 µM NADH2 and 25 mg/L HgCl2 and one volume of crude extract incubated at room temperature for various interval period of time. Mercury reductase activity was measured spectrophotometrically at 340 nm. One unit of reductase activity was defined as one molar of oxidized NADH2 produced per total cell per minute. Results of study showed that, the isolate could resist the concentration of HgCl2 up to 11 mg/L. At 30 minute incubation period at room temperature, the highest mercury reductase activity and the Hg2+ lowering capacity was found to be 0.006 unit/109cell and <br />1.48 mg/L/109cell/minute, respectively with the reduction efficiency of Hg2+ to Hg0 of 0.18% per minute. Therefore, it can be concluded that the Bacillus sp. S1 isolate could be assumed to be exellent mercury bioremediation agent since it was found to be highly mercury resistant and very efficient to reduce cationic mercury (Hg2+) to elemental mercury (Hg0). </p><p><strong>Keywords</strong>: Kalimas-Surabaya, Bacillus sp. S1, Mercury resistant, Mercury reductase</p>

scholarly journals Profil Protein Isolat Bakteri Resisten Merkuri Dari Pertambangan Emas Rakyat Di Desa Pongkor, Bogor-Jawa Barat, Indonesia

2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Patricia Wahyu Irawati
Keyword(s):  
Gold Mining ◽  
Bacterial Resistance ◽  
Inhibitory Concentration ◽  
Protein Profile ◽  
Mining Area ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Sds Page ◽  
Mercury Resistant Bacteria ◽  
Mic Value

Mercury pollution due to gold mining at Pongkor Village can threaten the life of organisms because mercury is toxic. Bioremediation can be considered to reduce mercury concentration in the water. The mercury-resistant bacteria can be isolated from the area and may be developed as mercury bioremediation agents. This study aims to isolate mercury-resistant bacteria, test their resistance to mercury, and conduct protein profile studies after mercury induction. The mercury-resistant bacteria were isolated from soil samples in the Pongkor Village gold mining area and the isolate resistance to HgCl2 was measured based on the Minimum Inhibitory Concentration (MIC) value. The protein profile was analyzed using SDS PAGE gel electrophoresis. The results showed that two of the most resistant bacterial isolates to mercury were HgP1 and HgP2 with MIC value was 575 ppm. The protein profile showed that mercury induced decreased protein synthesis under normal conditions and increased synthesis of one protein suspected to play a role in the mechanism of bacterial resistance to mercury.

Specificity of Lectins Labeled with Colloidal Gold to the Exopolymeric Matrix Carbohydrates of the Sulfate-Reducing Bacteria Biofilm Formed on Steel

2020 ◽  
Vol 82 (5) ◽  
pp. 11-20
Author(s):  
D.R. Abdulina ◽  
◽  
L.M. Purish ◽  
G.O. Iutynska ◽  
◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Colloidal Gold ◽  
Steel Surface ◽  
Lectin Binding ◽  
Sulfate Reducing Bacteria ◽  
Gold Particles ◽  
Sulfate Reducing ◽  
Transmission Electron ◽  
Reducing Bacteria

The studies of the carbohydrate composition of the sulfate-reducing bacteria (SRB) biofilms formed on the steel surface, which are a factor of microbial corrosion, are significant. Since exopolymers synthesized by bacteria could activate corrosive processes. The aim of the study was to investigate the specificity of commercial lectins, labeled with colloidal gold to carbohydrates in the biofilm exopolymeric matrix produced by the corrosive-relevant SRB strains from man-caused ecotopes. Methods. Microbiological methods (obtaining of the SRB biofilms during cultivation in liquid Postgate B media under microaerophilic conditions), biochemical methods (lectin-binding analysis of 10 commercial lectins, labeled with colloidal gold), transmission electron microscopy using JEM-1400 JEOL. Results. It was shown using transmission electron microscopy that the binding of lectins with carbohydrates in the biofilm of the studied SRB strains occurred directly in the exopolymerіс matrix, as well as on the surfaces of bacterial cells, as seen by the presence of colloidal gold particles. For detection of the neutral carbohydrates (D-glucose and D-mannose) in the biofilm of almost all studied bacterial strains PSA lectin was the most specific. This lectin binding in biofilms of Desulfotomaculum sp. К1/3 and Desulfovibrio sp. 10 strains was higher in 90.8% and 94.4%, respectively, then for ConA lectin. The presence of fucose in the SRB biofilms was detected using LABA lectin, that showed specificity to the biofilm EPS of all the studied strains. LBA lectin was the most specific to N-аcetyl-D-galactosamine for determination of amino sugars in the biofilm. The amount of this lectin binding in D. vulgaris DSM644 biofilm was 30.3, 10.1 and 9.3 times higher than SBA, SNA and PNA lectins, respectively. STA, LVA and WGA lectins were used to detect the N-acetyl-Dglucosamine and sialic acid in the biofilm. WGA lectin showed specificity to N-acetyl-D-glucosamine in the biofilm of all the studied SRB; maximum number of bounded colloidal gold particles (175 particles/μm2) was found in the Desulfotomaculum sp. TC3 biofilm. STA lectin was interacted most actively with N-acetyl-D-glucosamine in Desulfotomaculum sp. TC3 and Desulfomicrobium sp. TC4 biofilms. The number of bounded colloidal gold particles was in 9.2 and 7.4 times higher, respectively, than using LVA lectin. The lowest binding of colloidal gold particles was observed for LVA lectin. Conclusions. It was identified the individual specificity of the 10 commercial lectins to the carbohydrates of biofilm matrix on the steel surface, produced by SRB. It was estimated that lectins with identical carbohydrates specificity had variation in binding to the biofilm carbohydrates of different SRB strains. Establishing of the lectin range selected for each culture lead to the reduction of the scope of studies and labor time in the researching of the peculiarities of exopolymeric matrix composition of biofilms formed by corrosiverelevant SRB.

The influence of potassium dichromate on dissimilatory reduction of sulfate and nitrate ions by bacteria Desulfovibrio sp.

2017 ◽  
Vol 28 (1-2) ◽  
pp. 84-95
Author(s):  
O. M. Moroz ◽  
S. O. Hnatush ◽  
Ch. I. Bohoslavets ◽  
T. M. Hrytsun’ ◽  
B. M. Borsukevych
Keyword(s):  
Electron Acceptor ◽  
Potassium Dichromate ◽  
Anaerobic Respiration ◽  
Sulfate Reducing Bacteria ◽  
Negative Influence ◽  
Electron Acceptors ◽  
Metal Compounds ◽  
Nitrate Ions ◽  
Sulfate Reducing ◽  
Reducing Bacteria

Sulfate reducing bacteria, capable to reductive transformation of different nature pollutants, used in biotechnologies of purification of sewage, contaminated by carbon, sulfur, nitrogen and metal compounds. H2S formed by them sediment metals to form of insoluble sulfides. Number of metals can be used by these microorganisms as electron acceptors during anaerobic respiration. Because under the influence of metal compounds observed slowing of bacteria metabolism, selection isolated from technologically modified ecotops resistant to pollutions strains is important task to create a new biotechnologies of purification. That’s why the purpose of this work was to study the influence of potassium dichromate, present in medium, on reduction of sulfate and nitrate ions by sulfate reducing bacteria Desulfovibrio desulfuricans IMV K-6, Desulfovibrio sp. Yav-6 and Desulfovibrio sp. Yav-8, isolated from Yavorivske Lake, to estimate the efficiency of possible usage of these bacteria in technologies of complex purification of environment from dangerous pollutants. Bacteria were cultivated in modified Kravtsov-Sorokin medium without SO42- and FeCl2×4H2O for 10 days. To study the influence of K2Cr2O7 on usage by bacteria SO42- or NO3- cells were seeded to media with Na2SO4×10H2O or NaNO3 and K2Cr2O7 at concentrations of 1.74 mM for total content of electron acceptors in medium 3.47 mM (concentration of SO42- in medium of standard composition). Cells were also seeded to media with 3.47 mM Na2SO4×10H2O, NaNO3 or K2Cr2O7 to investigate their growth in media with SO42-, NO3- or Cr2O72- as sole electron acceptor (control). Biomass was determined by turbidymetric method, content of sulfate, nitrate, dichromate, chromium (III) ions, hydrogen sulfide or ammonia ions in cultural liquid – by spectrophotometric method. It was found that K2Cr2O7 inhibits growth (2.2 and 1.3 times) and level of reduction by bacteria sulfate or nitrate ions (4.2 and 3.0 times, respectively) at simultaneous addition into cultivation medium of 1.74 mM SO42- or NO3- and 1.74 mM Cr2O72-, compared with growth and level of reduction of sulfate or nitrate ions in medium only with SO42- or NO3- as sole electron acceptor. Revealed that during cultivation of bacteria in presence of equimolar amount of SO42- or NO3- and Cr2O72-, last used by bacteria faster, content of Cr3+ during whole period of bacteria cultivation exceeded content H2S or NH4+. K2Cr2O7 in medium has most negative influence on dissimilatory reduction by bacteria SO42- than NO3-, since level of nitrate ions reduction by cells in medium with NO3- and Cr2O72- was a half times higher than level of sulfate ions reduction by it in medium with SO42- and Cr2O72-. The ability of bacteria Desulfovibrio sp. to priority reduction of Cr2O72- and after their exhaustion − NO3- and SO42- in the processes of anaerobic respiration can be used in technologies of complex purification of environment from toxic compounds.

Removal of lead by sulfate-reducing bacteria in anaerobic continuous stirred tank reactors

2016 ◽  
Vol 14 (3) ◽  
pp. 557-561
Author(s):  
Nguyễn Thị Yên ◽  
Kiều Thị Quỳnh Hoa
Keyword(s):  
Sulfate Reducing Bacteria ◽  
Stirred Tank ◽  
Synthetic Wastewater ◽  
Terminal Electron Acceptor ◽  
Stirred Tank Reactors ◽  
Sulfate Reducing ◽  
Sulfide Production ◽  
Continuous Stirred Tank ◽  
Continuous Stirred Tank Reactors ◽  
Reducing Bacteria

Lead contaminated wastewater negatively impacts to living organisms as well as humans. In recent years, a highly promising biological process using the anaerobic production of sulfide ions by sulfate-reducing bacteria has presented itself as an alternative option for the removal of lead. This process is based on microbial utilization of electron donors, such as organic compounds (carbon sources), and sulfate as the terminal electron acceptor for sulfide production. The biogenic hydrogen sulfide reacts with dissolved heavy metals to form insoluble metal sulfide precipitates Removal of lead by an enriched consortium of sulfate-reducing bacteria (DM10) was evaluated sulfate reduction, sulfide production and lead precipitation. Four parallel anaerobic continuous stirred tank reactors (CSTR, V = 2L) (referred as R1 - R4) were fed with synthetic wastewater containing Pb2+ in the concentrations of 0, 100, 150 and 200 mg L-1 of lead and operated with a hydraulic retention time of 5 days for 40 days. The loading rates of each metal in R1- R4 were 0, 20, 30 and 40 mg L-1 d-1, respectively. The results showed that there was no inhibition of SRB growth and that lead removal efficiencies of 99-100% for Pb2+ were achieved in R2 (100 mg L-1) and R3 (150 mg L-1) throughout the experiment. For the highest lead concentration of  200 mg L-1, a decrease in efficiency of removal (from 100 to 96%) was observed at the end of the experiment. The obtained result of this study might help for a better control operation and performance improvements of reactors.

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