Structure Of Stigmasterols in Bran of Red Rice from Minahasa Regency, North Sulawesi, Indonesia

Rice bran contained important bioactive phytochemical components such as tocopherol, tocotrienol, and γ-oryzanol, and one of the components of γ-oryzanol is stigmasterol. The aim of the present study was to isolate and identify stigmasterol in native red rice bran extract. The isolation of sterol from red rice bran extract was performed by using Thin Layer Chromatography (TLC) and followed by identification by Fourier Transformed Infrared Spectroscopy (FTIR) and Nuclear Magnetic Resonance (NMR). The TLC single spot originated from crude red rice hexane extract was observed at 365 nm UV and the FTIR spectra analysis result showed that the OH group was indicated by 3436,9 cm-1 absorption. While absorption regions of 2931,6 to 2852,5 cm-1 indicate the presence of single bond CH. The data of 1H-, 13C- and 2D-NMR of rice bran sterol indicated that the single spot obtained in TLC analysis was stigmasterol. It can be concluded that native red rice variety of North Sulawesi contained stigmasterol and potential as a native antioxidant sources.

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ANTIOXIDANT EVALUATION AND PHYTOCHEMICAL CONTENT OF VARIOUS RICE BRAN EXTRACTS OF THREE VARIETIES RICE FROM SEMARANG-CENTRAL JAVA, INDONESIA

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i6.16565 ◽

2017 ◽

Vol 10 (6) ◽

pp. 377 ◽

Cited By ~ 1

Author(s):

Sukrasno Sukrasno ◽

Slamet Tuty ◽

Irda Fidrianny

Keyword(s):

Rice Bran ◽

Phenolic Content ◽

Antioxidant Activities ◽

Carotenoid Content ◽

Total Phenolic ◽

Red Rice ◽

Black Rice ◽

Dpph Assay ◽

Rice Bran Extract ◽

Dpph Scavenging

Objectives: The objectives of this research were to evaluate antioxidant activity from different polarities rice bran extract of three varieties of rice using two methods of antioxidant testing which were FRAP (Ferric Reducing Antioxidant Power) and DPPH (2,2-diphenyl-1-picrylhydrazyl), and correlation of total phenolic, flavonoid and carotenoid content with their EC50 of FRAP and IC50 of DPPH antioxidant activities. Methods: Extraction was conducted by reflux using different polarity solvents. The extracts were evaporated using rotary evaporator. Determination of total phenolic, flavonoid and carotenoid content, antioxidant activities using FRAP and DPPH assays were performed by UV-visible spectrophotometry and its correlation with EC50 of FRAP capacities and IC50 of DPPH scavenging activities were analyzed by Pearson’s method. Results: Ethanolic rice bran extract of black rice showed the lowest EC50 of FRAP capacity 64.35 µg/ml and IC50 of DPPH scavenging activity 23.92 µg/ml. The highest phenolic content, flavonoid content and carotenoid content were also given by ethanolic rice bran extract of black rice. There were significantly negative correlation between total phenolic content and carotenoid content in rice bran extract of red rice and black rice with their IC50 of DPPH. Conclusions: All of rice bran extracts (except n-hexane rice bran extract of black rice and ethanolic rice bran extract of white rice) were very strong antioxidant, by DPPH assay. Phenolic and carotenoid compounds in rice bran extracts of red rice and black rice were the major contributor in antioxidant activity by DPPH assay. Rice bran extracts of black rice had linear results by FRAP and DPPH assays.

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The Effect of Ethanol Extract 96% o Brown Rice Bran to the Number and Viability of White Rat (Rattus norvegicus) Spermatozoa Sprague dawley Strains Induced by Clove Cigarette Smoke

Berkala Kedokteran ◽

10.20527/jbk.v14i2.5327 ◽

2018 ◽

Vol 14 (2) ◽

pp. 133

Author(s):

Soraya Rahmanisa ◽

Natasya Hayatillah ◽

Evi Kurniawaty

Keyword(s):

Rice Bran ◽

Rattus Norvegicus ◽

Brown Rice ◽

Ethanol Extract ◽

Male Rats ◽

Red Rice ◽

Sperm Number ◽

Sprague Dawley ◽

Rice Bran Extract ◽

One Way Anova

Abstract: Indonesia has the greatest number of smokers in Southeast Asia dominated by male consume kretek cigarette. Cigarette’s smoke is source of free radicals that can cause oxidative stress to sperm and lead it to infertility. Red rice bran extract has lot of potential antioxidants to stop oxidatif stress. This study was experimental within 30 days. The 25 Sprague dawley male rats divided into 5 groups: K1 wasn’t treated, K2,P1,P2, and P3 exposed to smokes of 2 kretek cigarettes, given 96% ethanol extract of red rice bran dosage 100 mg/Kg (P1), 200 mg/Kg (P2) and 400 mg/Kg (P3). Spermatozoa number and viability was observed. Data tested with One Way Anova. There was significant effect from red rice bran extract toward sperm number and viability (p=0,00). Average spermatozoa number was 91.90±7.72 (K1), 39.68±7,51 (K2), 79.88±8.63 (P1), 86.40±10.5 (P2), 86.00±5.78 (P3). Average viability was 65.00±6.85 (K1), 29.6±5.85 (K2), 51.4±3.50 (P1), 60.00±6.67 (P2), 61,00±2.91 (P3). The increasing of number and viability has been achived at dose 100mg/Kg and best at 400 mg/KgBB. The 96% ethanol extract of red rice bran can prevent the decreasing number and viability of rat spermatozoa exposed by kretek cigarette. Keywords: Clove cigarette, spermatozoa, rice bran extract

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