scholarly journals ANTIOXIDANT EVALUATION AND PHYTOCHEMICAL CONTENT OF VARIOUS RICE BRAN EXTRACTS OF THREE VARIETIES RICE FROM SEMARANG-CENTRAL JAVA, INDONESIA

2017 ◽  
Vol 10 (6) ◽  
pp. 377 ◽  
Author(s):  
Sukrasno Sukrasno ◽  
Slamet Tuty ◽  
Irda Fidrianny
Keyword(s):  
Rice Bran ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Red Rice ◽  
Black Rice ◽  
Dpph Assay ◽  
Rice Bran Extract ◽  
Dpph Scavenging

Objectives: The objectives of this research were to evaluate antioxidant activity from different polarities rice bran extract of three varieties of rice using two methods of antioxidant testing which were FRAP (Ferric Reducing Antioxidant Power) and DPPH (2,2-diphenyl-1-picrylhydrazyl), and correlation of total phenolic, flavonoid and carotenoid content with their EC50 of FRAP and IC50 of DPPH antioxidant activities. Methods: Extraction was conducted by reflux using different polarity solvents. The extracts were evaporated using rotary evaporator. Determination of total phenolic, flavonoid and carotenoid content, antioxidant activities using FRAP and DPPH assays were performed by UV-visible spectrophotometry and its correlation with EC50 of FRAP capacities and IC50 of DPPH scavenging activities were analyzed by Pearson’s method. Results: Ethanolic rice bran extract of black rice showed the lowest EC50 of FRAP capacity 64.35 µg/ml and IC50 of DPPH scavenging activity 23.92 µg/ml. The highest phenolic content, flavonoid content and carotenoid content were also given by ethanolic rice bran extract of black rice. There were significantly negative correlation between total phenolic content and carotenoid content in rice bran extract of red rice and black rice with their IC50 of DPPH. Conclusions: All of rice bran extracts (except n-hexane rice bran extract of black rice and ethanolic rice bran extract of white rice) were very strong antioxidant, by DPPH assay. Phenolic and carotenoid compounds in rice bran extracts of red rice and black rice were the major contributor in antioxidant activity by DPPH assay. Rice bran extracts of black rice had linear results by FRAP and DPPH assays. 

scholarly journals Correlation of Total Phenolic, Flavonoid and Carotenoid Content of Sesbania sesban (L. Merr) Leaves Extract with DPPH Scavenging Activties

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Sani N. Fitriansyah ◽  
Irda Fidrianny ◽  
Komar Ruslan
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Sesbania Sesban ◽  
Total Carotenoid Content ◽  
Dpph Method ◽  
Dpph Scavenging

Antioxidants are found in many plants and can neutralize free radicals. Sesbania sesban (L.)Merr is a plant that has been used empirically by Indonesian people and its proven have many pharmacologycal activity. The objectives of this research were to study antioxidant activity Sesbania sesban leaves extract using DPPH (2,2-diphenyl-1-picrylhydrazyl) method and correlation with its total phenolic, flavonoid and carotenoid content. Extraction was performed by reflux using different polarity solvent. The extracts were vaporated using rotary evaporator. Antioxidant activity was tested using DPPH assay. Determination of total phenolic, flavonoid and carotenoid content were performed by UV-visible spectrophotometry and its correlation with inhibitory concentration 50 (IC50) DPPH scavenging activity were analyzed by Pearson’s method. Nhexane, ethyl acetate and ethanol extracts of Sesbania sesban leaves had IC50 DPPH  50 g/ml and it can be classified as very strong antioxidant. Ethanol extract of Sesbania sesban had the highest of total phenolic content (5.18 g GAE/100 g) and highest total flavonoid content (4.56 g QE/100 g), while the highest total carotenoid content (4.56 g BE/100 g) was given by n-hexane extract. Total phenolic content in Sesbania sesban leaves extracts had significant and negative correlation with their IC50 DPPH scavenging activities. Phenolic compounds in Sesbania sesban leaves extracts were contributor major in its antioxidant activities by DPPH method.

Phenolic Content, Antibacterial and Antioxidant Activities of Moroccan propolis

2020 ◽  
Vol 15 (6) ◽  
pp. 696-705
Author(s):  
Omar Belmehdi ◽  
Badia Douhri ◽  
Abdelhakim Bouyahya ◽  
Yousif Laghmouchi ◽  
Nadia S. Senhaji ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Capacity ◽  
Bleaching Test ◽  
Ethanol Extracts ◽  
Dpph Scavenging

Background:Propolis contains compounds with antibacterial and antioxidant capacities, such as phenolic compounds and flavonoids. The aim of this study was to investigate the antibacterial and antioxidant effects of six propolis samples from northern Morocco and to determine their content of polyphenols and flavonoids.Methods:The total polyphenols and flavonoids of Ethanol Extracts (EEP) were evaluated by colorimetric assays. To test the antibacterial activity, the well diffusion and micro-dilution methods were adopted. The kinetic of bacterial growth in the presence of EEP was tested. The antioxidant activity was evaluated using three techniques: reducing power, β-carotene bleaching test, and DPPH scavenging capacity assay.Results:The EEPs were found to have high total phenolic content (65.09 - 122.00 mg GAE/g) and flavonoids (41.95 - 50.44 mg QE/g). The screening of the antibacterial activity showed that all tested bacteria were sensitive to EEP except Escherichia coli. The inhibitory zones varied from 11.50 ± 0.70 to 32.00 ± 0.70 mm, and MIC and MBC ranged from 0.07 to 5 mg/mL and 0.07 to >5 mg/mL, respectively. The antioxidant activity was proportional to the phenolic content of propolis. The IC50 varied from 48.70 to 156.00 mg/mL and the RAA% ranged from 75.07 to 96.68 %.

scholarly journals Effect of grinding and extraction conditions on the determination of antioxidant activity and phenolic acids in barley

2021 ◽  
Author(s):  
Bing Zhou ◽  
Zhao Jin ◽  
Paul B. Schwarz ◽  
Yin Li
Keyword(s):  
Phenolic Acid ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Dpph Scavenging

AbstractIn the present study, the objective was to evaluate the effects of barley kernel size, grind level, and extract solvent on the antioxidant activities associated with total phenolic content and phenolic acid compositions. Three barley varieties (Kindred, Azure, and Tradition), were used and the results showed that with the exception of superoxide anion radical scavenging activity, thin kernel size fraction (2.0 mm) showed much higher antioxidant activities and total phenolic content than those in the 2.4 mm and 2.8 mm fractions when averaged across barley cultivar, grind level, and extract solvent. A similar trend was found for individual phenolic acid compositions among kernel sizes. Simple correlation analysis revealed that total phenolic content showed strong correlation with DPPH· scavenging activity, ABTS + scavenging activity, and reducing power. Generally, fine grind and 80% acetone extract showed averaged highest values in antioxidant activities, total phenolic content, and individual phenolic acid compositions. Stepwise linear regression showed that extract solvent was the most important factor for DPPH· scavenging activity, ABTS + scavenging activity, reducing power, and iron chelating activity. The sum of syringic acid and caffeic acid exhibited a dominant role in explaining the major variation in antioxidant activities except for superoxide anion radical scavenging activity.

scholarly journals Antimicrobial, Antioxidant and Cytotoxic Activities of Glochidion multiloculare (Roxb. ex Willd.) Müll. Arg. (Euphorbiaceae)

2013 ◽  
Vol 11 (2) ◽  
pp. 117-120
Author(s):  
ATM Zafrul Azam ◽  
Abdullah Al Hasan ◽  
Md Gias Uddin ◽  
Mohammad Mehedi Masud ◽  
Choudhury Mahmood Hasan
Keyword(s):  
Brine Shrimp ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Ic50 Value ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Liquid Chromatographic

The methanol extract (ME) of the powdered bark of Glochidion multiloculare and its six vacuum liquid chromatographic (VLC) fractions (Fa-f) were investigated for antimicrobial, cytotoxic and antioxidant activities. Only fractions Fc and Fd showed mild antimicrobial activity. Significant free radical (DPPH) scavenging activity was found in Ff (IC50 value = 9.27±0.117 ?g/ml). The total phenolic content was measured involving Folin-Ciocalteu reagent and it was the highest in fraction Fe (187.00±1.74 mg of GAE/gm of sample). Cytotoxicity (LC50) by brine shrimp lethality bioassay was found to be significant for Fb (0.023±0.001 ?g/ml), Fc (0.3±0.01 ?g/ml) and Fd (0.117±0.0015 ?g/ml). DOI: http://dx.doi.org/10.3329/dujps.v11i2.14560 Dhaka Univ. J. Pharm. Sci. 11(2): 117-120, 2012 (December)

scholarly journals Antioxidant profile of chicken bile extract and correlation with chemical content

2019 ◽  
Vol 10 (3) ◽  
pp. 2311-2317
Author(s):  
Tuty S ◽  
Fidrianny I ◽  
Sukrasno
Keyword(s):  
Phenolic Content ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Dpph Assay ◽  
Dpph Method ◽  
Bile Extract ◽  
Dpph Scavenging ◽  
Bear Bile ◽  
Phenolic And Flavonoid

Component in chicken bile may be similar to bear bile, which contains an immunostimulant agent ursodeoxycholic acid and related to antioxidant effect. The purposes of this study were to evaluate the antioxidant potential of chicken bile extract by the DPPH method, analyze the correlation between total phenolic and flavonoid content with IC50 of DPPH scavenging activities. Chicken bile was cut and dried using freeze dryer, then extracted by reflux using different polarity solvents. A rotary evaporator was used to concentrate the extracts. Calculation of total flavonoid and phenolic content, antioxidant potential using DPPH assay were carried out by UV-visible spectrophotometry, and Pearson’s method was used to analyze their correlation. IC50 of DPPH of ethanolic chicken bile extract was 46.64 µg/ml, while ethyl acetate and n-hexane extracts were 69.99 µg/ml and IC50 71.65 µg/ml, respectively. The highest phenolic content and flavonoid content were given by ethanolic chicken bile extract. The total phenolic and flavonoid content in chicken bile extracts had a significant and negative correlation with IC50 of DPPH. All chicken bile extracts are potential antioxidants by DPPH assay. Phenolic and flavonoid compounds in chicken bile extracts contributed together in antioxidant capacity by DPPH assay.

scholarly journals Quantification of Phytochemicals Imparting Antioxidant Activities in Commonly Used Vegetables

2018 ◽  
Vol 6 (2) ◽  
pp. 97-102
Author(s):  
Ram Lakhan Singh ◽  
Pankaj Singh
Keyword(s):  
Ascorbic Acid ◽  
Protein Content ◽  
Total Phenolic Content ◽  
Allium Sativum ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Cucurbita Maxima ◽  
Age Related

Plant derived phytochemicals have recently became of great importance in the protection of various diseases, like heart disease, cardiovascular disease, cancer, diabetes, Alzheimer’s disease, cataract and age related functional disorders caused by free radicals. The present study was carried out to explore the commonly used vegetables having higher content of antioxidant imparting phytochemicals such as ascorbic acid, carotenoids, total phenolic content, carbohydrate and protein content in commonly used vegetables. The results revealed that the concentration among tested samples ranged from 7.07 mg/100g of FW (Momardica charantia leaf) to 174.15 mg/100g of FW (Allium sativum leaf) for ascorbic acid; 1.31 µg/g of FW (Chenopodium album leaf) to 14.00 µg/g of FW (Allium sativum leaf ) for carotenoid content; 8.72 mg of GAE/g of DW (Cucurbita maxima fruit) to 67.20 mg/g of DW (Colocasia esculentum leaf) for total phenolic content; 27.15 mg/g (Laginaria vulgaris leaf) to 901.00 mg/g (Cucurbita maxima fruit) for carbohydrate content and 35.96 mg/g (Amarphophyllus fruit) to 589.23 mg/g (Beta vulgaris fruit) for protein content. Results also showed that these bioactive phytochemicals are widely distributed in the vegetables and their concentrations are variable in different vegetables as well as vegetable part’s itself. Hence, vegetable rich diet having higher content of phytochemicals can be used to cure or in the prevention of various chronic diseases such as hepatotoxicity, diabetes, cardiovascular diseases, cancer, oxidative stress etc and may serve as a good source of nutraceuticals which have potential for use in health care formulations.Int. J. Appl. Sci. Biotechnol. Vol 6(2): 97-102

scholarly journals PHYTOCHEMICAL CONTENT AND ANTIOXIDANT ACTIVITIES IN DIFFERENT ORGANS OF POMELO (CITRUS MAXIMA [BURM.] MERR.) USING 2,2-DIPHENYL-1-PICRYLHYDRAZYL AND PHOSPHOMOLYBDENUM ASSAYS

2016 ◽  
pp. 185 ◽  
Author(s):  
Irda Fidrianny ◽  
Elvira Sari ◽  
Komar Ruslan
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Phytochemical Content ◽  
Dpph Method ◽  
Citrus Maxima ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Peel Extract

<p>ABSTRACT<br />Objectives: The aims of this research were to determine antioxidant activity from various organs extracts of pomelo using 2,2-diphenyl-1picrylhydrazyl<br />(DPPH)<br />and<br />phosphomolybdenum<br />assays,<br />total<br />phenolic,<br />flavonoid,<br />and<br />carotenoid<br />content,<br />correlation<br />of<br />total<br />phenolic,<br />flavonoid,</p><p>and<br />carotenoid<br />content<br />in<br />various<br />extracts<br />of<br />chayote<br />with their<br />inhibitory<br />concentration<br />50%<br />(IC<br />) of DPPH antioxidant activities and exhibitory<br />concentration 50% (EC<br />50<br />) of phosphomolybdenum capacity, and correlation between two antioxidant assays.<br />50<br />Methods: Extraction was carried out by reflux using various polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidant<br />activities using DPPH and phosphomolybdenum assays, determination of total phenolic, flavonoid, and carotenoid content were conducted by UVvisible<br />spectrophotometry<br />and its correlation<br />with IC<br />Results: The lowest IC<br />50<br />50<br /> of DPPH and EC<br />50<br /> of phosphomolybdenum was analyzed by Pearson’s method.<br /> of DPPH scavenging activity was shown by ethyl acetate cortex extract of pomelo (0.68 µg/ml), whereas the lowest EC<br /> of<br />phosphomolybdenum capacity was given by ethyl acetate leaves extract of pomelo (101.36 µg/ml). Ethyl acetate cortex extract of pomelo had the<br />highest total phenolic content and ethyl acetate leaves extract had the highest total flavonoid content (TFC). There was a negative and significant<br />correlation between TFC in cortex and peel extracts of pomelo with their IC<br />50<br /> of DPPH.<br />Conclusions: All organs extracts of pomelo (except n-hexane peel extract) were classified as a very strong antioxidant by DPPH method. Flavonoid<br />compounds in cortex and peel extract of pomelo were the major contributor in antioxidant activities by DPPH method. DPPH and phosphomolybdenum<br />assays gave no linear results in antioxidant activities of leaves, cortex, and peel extracts of pomelo.<br />Keywords: Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Phosphomolybdenum, Pomelo, Three organs.<br />50</p>

Screening of Microalgae for Antioxidant Activities, Carotenoids and Phenolic Contents

2014 ◽  
Vol 625 ◽  
pp. 156-159 ◽  
Author(s):  
Hamdy Elsayed Ahmed Ali ◽  
Sanaa Mahmoud Metwally Shanab ◽  
Mervat Aly Mohamed Abo-State ◽  
Emad Ahmed Ahmed Shalaby ◽  
Usama Eldmerdash ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Scenedesmus Obliquus ◽  
Ethanolic Extract ◽  
Natural Antioxidants ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Microalgal Biomass ◽  
Phenolic Contents ◽  
Dpph Scavenging

Ten different microalgal species were screened for their antioxidant activity using 2,2 ́-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay, both total phenolic and carotenoid contents were determined. The ethanolic extract ofOscillatoriasp. showed the highest antioxidant activity (69.1 %), while the greenChlorellasp. showed higher phenolic content (39.1 mg GAE g-1dry wt.) andScenedesmus obliquusrecorded higher carotenoid content (3.73 mg L-1), than cyanobacterial species Microalgal biomass plays a great role as a potential source of natural antioxidants, not only due to the carotenoids but also the phenolic compounds.

scholarly journals TOTAL PHENOLIC CONTENT, ANTIOXIDANT CAPACITY AND STARCH HYDROLYTIC ENZYME INHIBITION OF THE EXTRACTS FROM UNPOLISHED COLORED RICES

2018 ◽  
Vol 15 (4) ◽  
pp. 685-690
Author(s):  
Le Hoang Dao ◽  
Pham Van Hung
Keyword(s):  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Free Form ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Red Rice ◽  
Flavonoid Content ◽  
Black Rice ◽  
White Rice

The different colors of rice are contributed by various phenolic compounds, which have various health benefits. However, phenolic compounds, which are mostly existed in rice bran and easily lost during milling. Therefore, the consumption of unpolished rice is recommended to prevent from many diseases. The objective of this study is to determine total phenolic and flavonoid contents, antioxidant capacity and diabetic enzyme inhibition potential of the extracts of the different unpolished colored rices. Three kinds of unpolished colored rices (unpolished white rice, unpolished red rice and unpolished black rice) grown in Vietnam were used to extract their bioactive compounds by alcoholic solvent extracting method. The results showed that the highest level of total phenolic content (TPC) was found in unpolished red rice (470.96 µg FAE/g db sample), while the lowest was in unpolished white rice (135.84 µg FAE/g db sample). TPC of rice in free form was considerably higher than that in the bound form. Total flavonoid content in free form was also significantly higher than bound form. Total flavonoid content in free form of unpolished black rice (126.75 µg RE/g db sample) was higher than that in the others. In the present study, unpolished red rice extract possessed the best antioxidant activity (78.79%) in most cases, and showed the strongest inhibitory potential against alpha-amylase (78.56%) and amyloglucosidase (36.13%) as well. As a result, extracts of unpolished colored rices could act like both antioxidant and enzyme inhibitors and have a potential to be used in prevention of type 2 diabetes.

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