scholarly journals COMPARISON OF HBA1C LEVEL MEASURED BY HPLC AND CAPILLARY ELECTROPHORESIS AMONG PATIENT WITH HIGH UREA

2021 ◽  
Vol 5 (1) ◽  
pp. 56-60
Author(s):  
Wan Nor Fazila Hafizan Wan Nik ◽  
Iwani Abd Razak ◽  
Noor Azlin Azraini Che Soh ◽  
Noorazliyana Shafii ◽  
Julia Omar ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Capillary Electrophoresis ◽  
Hba1c Level ◽  
High Performance ◽  
Comparative Method ◽  
Glycated Haemoglobin ◽  
Routine Method ◽  
Urea Level ◽  
The Mean ◽  
Hba1c Measurement

Utilization of glycated haemoglobin (HbA1c) in diagnosis and monitoring of diabetes mellitus is accepted and validated worldwide. Standardisation between various methods available is no longer an issue. However, knowledge of HbA1c interference by various haemoglobin (Hb) fractions presence in the patient’s sample must be taken into account during HbA1c analysis and interpretation. Carbamylated Hb (cHb) is one of Hb fractions, formed when Hb condensed at the N-terminal valine by cyanate derived from spontaneous decomposition of urea which usually raised in patients with renal impairment. This study aimed to compare the level of HbA1c in patient with high urea measured using High Performance Liquid Chromatography (HPLC) and Capillary Electrophoresis (CE). After analysis using the laboratory’s routine method, or HPLC, the patient’s samples with concurrent urea level of >25 mmol/L were re-analyzed within 2 hours using the comparative method or CE.  A cut off cHb of 2% on HPLC considered as no interference. The mean level of urea was 31.37±5.09 mmol/L (range 25.2-43.1mmol/L). Out of 68 samples, only 24 cHb were detected by HPLC but only less than 2% and none cHb detected on CE.  Correlation between HPLC and CE showed no significant different in HbA1c measurement (r= p>0.05). Therefore, we propose that both HPLC and CE can be used to determine HbA1c level in patient with high urea.

scholarly journals The relation of anatomical distribution of symptomatic peripheral arterial disease (PAD) with HbA1c level in patients with type 2 diabetes mellitus

2021 ◽  
Vol 12 ◽  
pp. 204201882110005
Author(s):  
Nawaf J. Shatnawi ◽  
Nabil A. Al-Zoubi ◽  
Hassan M. Hawamdeh ◽  
Yousef S. Khader ◽  
Mowafeq Heis ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Peripheral Arterial Disease ◽  
Hba1c Level ◽  
Arterial Disease ◽  
Anatomical Distribution ◽  
The Mean ◽  
Peripheral Arterial

Aims: Increased level of glycated hemoglobin (HbA1c) is associated with an increased prevalence of peripheral arterial disease (PAD). This study aimed to assess the relationship between the anatomical distribution of symptomatic PAD lesions in patients with type 2 diabetes and HbA1c levels at the time of PAD diagnosis. Patients and methods: A retrospective study was conducted at King Abdullah University Hospital during the period August 2011 to December 2015. Consecutive patients with type 2 diabetes presented with symptomatic PAD confirmed by computed tomography-angiography (CTA) were included in this study. CTA images were reviewed. Relevant information including demographic data, PAD symptoms, comorbidities, HbA1c level, lipid profile, C-reactive protein and the mean platelets volume were retrieved from medical records. Results: A total of 332 patients with type 2 diabetes (255 males and 77 females) were included in this study. The mean HbA1c at the time of PAD diagnosis was 8.68% (±2.06%). The prevalence of hemodynamic relevant atherosclerotic lesions of the superficial femoral artery, popliteal artery, leg vessels, femoro-popliteal, and crural segments was significantly higher in patients with HbA1c >7.5% compared with patients with HbA1c ⩽7.5%. Conclusion: The anatomical distribution of symptomatic PAD in patients with type 2 diabetes mellitus differed significantly according to HbA1c level at the time of PAD diagnosis.

HBA1C IS ASSOCIATED WITH FRAILTY IN A GROUP OF ABORIGINAL AUSTRALIANS

2018 ◽  
pp. 1-4
Author(s):  
Z. Hyde ◽  
K. Smith ◽  
L. Flicker ◽  
D. Atkinson ◽  
S. Fenner ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Risk Factor ◽  
Hba1c Level ◽  
Binary Logistic Regression ◽  
Glycated Haemoglobin ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Aboriginal Australians ◽  
Obesity And Diabetes ◽  
Hba1c Levels

In this cross-sectional study of 141 Aboriginal Australians aged ≥45 years living in the remote Kimberley region of Western Australia, we explored whether glycated haemoglobin (HbA1c) levels were associated with frailty. Sixty-four participants (45.4%) had a HbA1c level ≥6.5% and 84 participants (59.6%) were frail. A significant trend was observed with regard to HbA1c levels and frailty, with those having HbA1c levels ≥6.5% having the greatest prevalence of frailty (70.3%). In binary logistic regression analyses, having a HbA1c level ≥6.5% was associated with being frail after adjustment for age, sex, and education. This association was attenuated after further adjustment for body mass index (BMI). Poorer glycaemic control is very common and a potential risk factor for frailty in remote-living Aboriginal Australians, and appears to be partly mediated by BMI, a known risk factor for diabetes mellitus. Obesity and diabetes mellitus are potentially important modifiable risk factors for frailty.

scholarly journals Factors associated with high levels of glycated haemoglobin in patients with type 1 diabetes: a multicentre study in Brazil

BMJ Open ◽  
2017 ◽  
Vol 7 (12) ◽  
pp. e018094 ◽  
Author(s):  
Carine Sousa Andrade ◽  
Guilherme Sousa Ribeiro ◽  
Carlos Antonio Souza Teles Santos ◽  
Raimundo Celestino Silva Neves ◽  
Edson Duarte Moreira
Keyword(s):  
Type 1 Diabetes ◽  
Glycaemic Control ◽  
Hba1c Level ◽  
Glycated Haemoglobin ◽  
Poor Adherence ◽  
Adherence To Diet ◽  
The Mean ◽  
Lower Education ◽  
Hba1c Levels

ObjectiveLong-term complications of type 1 diabetes mellitus (DM1) can be prevented with adequate glycaemic control. However, high levels of glycated haemoglobin (HbA1c) occur in 60%–90% of the patients with DM1. Thus, we aimed to investigate the role of sociodemographic, behavioural and clinical factors on the HbA1c levels of patients with DM1 in Brazil.Design, setting and participantsA cross-sectional study was conducted in ambulatory patients with DM1 aged ≥18 years from 10 Brazilian cities. Sociodemographic, behavioural and clinical data were obtained through interviews.Main outcome measuresHbA1c level was measured by liquid chromatography. Hierarchical multiple variable linear regression models were used to identify factors correlated with high levels of HbA1c.ResultsOf 979 patients with DM1, 63.8% were women, and the mean age was 40 (SD 14.6) years. The mean HbA1c level was 9.4% (SD 2.2%), and 89.6% of the patients had HbA1c ≥7.0%. Factors independently correlated with increased HbA1c levels included: lower education, non-participation in diabetes classes/lecture during the year before, having a self-perception of poor adherence to diet and insulin, not having private medical care and not measuring the HbA1c levels in the prior year. Of note, poor adherence to diet and insulin were the independent factors most strongly associated with high levels of HbA1c (mean increment in HbA1c levels of 0.88% and 1.25%, respectively).ConclusionPoor glycaemic control, which is common among Brazilian patients with DM1, is associated with lower education, self-perception of insufficient adherence to diet and insulin and inadequate monitoring of HbA1c levels. Specific actions, particularly those targeting improving adherence to diet and insulin, may contribute to successful management of patients with DM1.

Short-Term Effect of Hypergastrinemia Following Esomeprazole Treatment On Well-Controlled Type 2 Diabetes Mellitus: A Prospective Study

2020 ◽  
Vol 20 (7) ◽  
pp. 1090-1096
Author(s):  
Yusuf Bozkuş ◽  
Umut Mousa ◽  
Özlem T. İyidir ◽  
Nazlı Kırnap ◽  
Canan Ç. Demir ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Hba1c Level ◽  
Intervention Group ◽  
Control Group ◽  
Metformin Monotherapy ◽  
The Mean ◽  
Hba1c Levels

Objective: Proton pump inhibitor (PPI) drugs reduce gastric acid secretion and lead to an increase in serum gastrin levels. Many preclinical and some clinical researches have established some positive effects of gastrin or PPI therapy on glucose regulation. The aim of this study was to prospectively investigate the short term effects of esomeprazole on glycaemic control in patients with type 2 diabetes mellitus. In addition, the presence of an association between this effect and gastrin levels was evaluated. Methods: Thirty-two subjects with type 2 diabetes mellitus were enrolled and grouped as intervention (n=16) and control (n=16). The participants in the intervention group were prescribed 40 mg of esomeprazole treatment for three months. At the beginning of the study and at the 3rd month, HbA1c level (%) and gastrin levels (pmol/L) of participants were assessed. Then, the groups were compared in terms of their baseline and 3rd month values. Results: In the intervention group, the mean gastrin level increased significantly from 34.3±14.4 pmol/L to 87.4±43.6 pmol/L (p<0.001). The mean HbA1c level was similar to the pre-treatment level (6.3±0.7% vs. 6.4±0.9%, p=0.441). There were no statistically significant differences in all parameters of the control group. The majority of individuals were on metformin monotherapy (65.6 %). The subgroup analysis of metformin monotherapy revealed that, in intervention group, there was a significant increase in gastrin levels (39.9±12.6 vs. 95.5±52.5, p=0.026), but the HbA1c levels did not change (6.0±0.4 % vs. 5.9±0.6 %, p=0.288); and in control group, gastrin levels did not change (37.5 ± 26.7 vs. 36.1 ±23.3, p=0.367), but there was an increase in HbA1c levels (6.1 ± 0.50 vs. 6.4 ± 0.60, p=0.01). Conclusion: Our study demonstrates that esomeprazole has no extra benefit for the controlled diabetic patient in three months. However, in only the metformin-treated subgroup, esomeprazole may prevent the rise in HbA1c level.

The effectiveness and safety of the supplementation of therapy with oral hypoglycemic agents by basal insulin in the patients with type 2 diabetes mellitus. Results of the SOLOS observational program

2013 ◽  
Vol 59 (4) ◽  
pp. 65-68
Author(s):  
M G Pavlova ◽  
I V Glinkina
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Insulin Glargine ◽  
Hba1c Level ◽  
Combined Therapy ◽  
Fasting Blood Glucose ◽  
Hypoglycemic Agents ◽  
The Mean

The results of numerous clinical investigations carried out during the last years provide convincing evidence of the high effectiveness and safety of early initiation of insulin therapy. Of special interest are the studies conducted under conditions of real clinical practice. One of them is the Russian SOLOS observational program designed to evaluate the influence of intensification of the treatment of type 2 diabetes mellitus with oral hypoglycemic agents (OHA) supplemented by using insulin glargine in the patients who failed to be adequately compensated by OHA therapy alone. Another objective of this program was to find out the opinion of the patients and physicians about the SoloStar pen employed for injecting insulin glargine. The data concerning 1309 patients at the mean age of 59.5±8.8 years with type 2 diabetes mellitus and the mean duration of the disease 6.9±5.0 years were available for the analysis. The overwhelming majority of the patients presented with severe decompensation of carbohydrate metabolism, the mean fasting blood glucose level being 10.7±2.4 mmol/l and the HbA1c level varying from 7.2 to 18.2% (mean 9.6±1.5%). Supplementation of OHA therapy with insulin glargine caused the significant reduction of the HbA1c level to 8.1±1.0% (p<0.001) within 3 months after the onset of the combined treatment. The HbA1c level fell down to 7.2±0.9% (p<0.001) after 6 months 40.6% of the patients had HbA1c at the level below 7%. Fasting blood glucose levels decreased to 7.4±1.4 mmol/l and 6.3±1.0 mmol/l (p<0.001) at 3 and 6 months after the onset of insulin administration respectively. The number of daytime and nocturnal hypoglycemic episodes under effect of insulin glargine injections significantly decreased by the end of the study. Severe hypoglycemic episodes suffered initially by 8 (0.5%) patients totally disappeared 6 months after the initiation of the combined therapy. Most patients did not need hospitalization after supplementation of OHA treatment with insulin. The results of combined therapy with the use of disposable SoloStar pens were positively assessed by the overwhelming majority of both the patients and the physicians involved in the study.

scholarly journals Modern approaches to the treatment of type 2 diabetes mellitus and its complications

2012 ◽  
Vol 58 (3) ◽  
pp. 61-64
Author(s):  
A S Ametov ◽  
E V Doskina
Keyword(s):  
Diabetes Mellitus ◽  
Hba1c Level ◽  
Platelet Adhesion ◽  
International Diabetes Federation ◽  
The Other ◽  
The World ◽  
The Mean ◽  
The One ◽  
Hba1c Levels

According to the International Diabetes Federation, there are over 366 mln subjects suffering diabetes mellitus (DM) in the world. This figure is expected to reach 552 mln by 2030. The patients are treated with preparations acting on different components of DM pathogenesis on the one hand and with medicines facilitating prophylaxis of the disease on the other hand. Diabeton MB decreases the mean HbA1c level of 7-8% by 0.9%. Also, it causes reduction of the initial HbA1c levels of 8-9% and 10% by 1.7% (2.6---??) and 4.2% respectively. Diabeton MB produces a number of other effects besides the hypoglycemic activity; specifically, it reduces the intensity of LDLP oxydation, platelet adhesion and aggregation, adhesion of monocytes, etc.

scholarly journals Relationship Of Glycated Haemoglobin (Hba1c) And Glucose In Streptozotocininduced Wistar Rats Is Determined By Linear Regression

2013 ◽  
Vol 3 (3) ◽  
pp. 1-5
Author(s):  
George Gborienemi Simeon ◽  
AA Uwakwe ◽  
GO Ibeh
Keyword(s):  
Diabetes Mellitus ◽  
Plasma Glucose ◽  
High Performance ◽  
Glycated Hemoglobin ◽  
Medical Science ◽  
Glycated Haemoglobin ◽  
Citrate Buffer ◽  
Liquid Chromatograph ◽  
Mean Values ◽  
Relationship Of

Objective To evaluate the relationship of glucose and glycated haemoglobin (HbA1C) in type 1 diabetes model induced by streptozotocin. Research Design and Methods Induction of diabetes mellitus was achieved through the intraperitoneal injection of 70mg/kg body weight of streptozotocin dissolved in 1m citrate buffer pH 4.5 twice daily for 2 days. A total number of thirty rats were used selected among those that have exceeded glucose threshold (>10.0mmol/l) 2 weeks after streptozotocin induction. All rats weighed between 240-300g. Samples for fasting plasma glucose and glycated haemoglobin were collected at the tail vein. Glucose was determined by the glucose oxidase method and HbA1C was determined by High Performance Liquid Chromatograph (HPLCEsi/ ms) with uv detection. Data was analysed by one way and two way analysis of variance using SPSS version. Results Significant linear relationship was demonstrated between plasma glucose level and glycated haemoglobin which could be predictive of risk of developing diabetes. Control samples had values within reference range, glucose (3.5-6.5 mmol/l) and glycated hemoglobin (4.3-7%). However diabetic test rats elicited values that varied significantly with time. Test result confirms the fact that higher mean values of plasma glucose in diabetic (positive) controls were due to the effect of streptozotocin. Conclusion Plasma glucose and glycated hemoglobin show positively mutual relationship and can be used in early diagnosis of diabetes mellitus. Using correlation coefficient and regression enhances measurement of the strength of the bivariate association and is predictive. Asian Journal of Medical Science, Volume-3 No-3 (2012), Page 1-5 DOI: http://dx.doi.org/10.3126/ajms.v3i3.4816

scholarly journals PROFIL ASAM LAKTAT PENDERITA DIABETES MELLITUS TERKENDALI (KONTROL) DAN TIDAK TERKENDALI (KONTROL)

2018 ◽  
Vol 14 (3) ◽  
pp. 97
Author(s):  
Laily Indrayanti ◽  
Harjo Mulyono
Keyword(s):  
Diabetes Mellitus ◽  
Lactic Acid ◽  
Hba1c Level ◽  
Acid Level ◽  
Pearson Correlation ◽  
Diabetic Patients ◽  
Lactic Acid Level ◽  
Group I ◽  
The Mean ◽  
Group Ii

Lactic acid is an intermediate product of carbohydrate metabolism. Increment of plasma lactic acid level usually correlated withaerobic metabolism defect which caused by hypoperfusion or hypoxia, that can be happened in DM (Diabetes Mellitus) patients. DiabetesMellitus is a risk factor of lactic acidosis. Determination of glycated Hb (HbA1c) is a parameter to monitor the blood glucose. The aimof this study is to compare the mean lactic acid level between uncontrolled and controlled DM) patients and their correlation betweenlactic acid and HbA1c level. The research carried out by cross sectional study which was done at the Clinical Pathology Laboratory ofSardjito Hospital between September–October 2007. Inclusion criteria of samples were diabetic patients who had HbA1c examination.Statistical analysis was done by independent t test and Pearson correlation test. Twenty one patients were included in this research.They were divided into two (2) groups, group I are those who had HbA1c ≤ 7%, they consist of 10 patients, group II are patients whohad HbA1c ≥ 7.1%, they consist 11 patients. The mean of lactic acid of group I was 1.85 mmol/L and group II was 1.74 mmol/L (p = 0.574). There wasn’t any significant correlation between HbA1c level and lactic acid. (r = -0.179, p = 0.437). The mean of lacticacid level in uncontrolled DM was lower than the controlled one but not significant, and there was no significant correlation betweenHbA1c level and lactic acid. It is suggested to continue this study but with larger sample to know the correlation between lactic acid andHbA1c in DM patients who had metformin therapy.

scholarly journals Estimation of Glycated Haemoglobin by Nephelometry, Ion Exchange Resin and High Performance Liquid Chromatography: A Cross-sectional Study

2020 ◽  
Author(s):  
Mangalore Balakrishna Prathima ◽  
Shridhar Reshma ◽  
. Sushith ◽  
Prathiksha Shetty ◽  
D'Sa Janice ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ion Exchange ◽  
Gold Standard ◽  
High Performance ◽  
Glycated Haemoglobin ◽  
Diabetic Patients ◽  
Cross Sectional ◽  
The Mean ◽  
R Value

Introduction: Diabetes mellitus cases are continually rising all over the world. Glycated haemoglobin (HbA1c) used as a diagnostic test to measure long-term average glycaemic control in diabetic patients. Aim: To assess the precision and reproducibility of the Ion Exchange Column Chromatography Resin (IECR) method and nephelometry in comparison to High-Performance Liquid Chromatography (HPLC) with respect to the estimation of HbA1c. Materials and Methods: A cross-sectional comparative study was conducted on 50 blood samples, collected from the diabetic subjects at the Department of Biochemistry and their HbA1c values were estimated by HPLC based BioRad D-10, nephelometry and IECR techniques. HPLC was used as a gold standard method, to evaluate the sensitivity and specificity of nephelometry and IECR techniques. Data were expressed as mean±standard deviation and intraclass Correlation Coefficient and Pearson correlation were calculated. Results: The mean age of the study subjects was 53.06±7.67 years. The mean plasma HbA1c levels were 8.16±2.9, 7.62±2.5 and 7.84±2.5 and mean estimated Average Glucose (eAG) was 187.00±84.4, 172.0±76.8, and 289.46±199.9 by HPLC, Nephelometry and IECR, respectively. Compared with HPLC, nephelometry had excellent correlation (r-value 0.925); p<0.001) and IECR (r-value 0.869; p<0.001). Conclusion: Nephelometry and IECR both had better performance and showed a greater concordance with gold standard HPLC. Therefore, nephelometry and IECR can be used as an alternative assay for HbA1c estimation.

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