scholarly journals Effects of Nitrogen Sources in Phytase Production on Bacterial Strains Isolated from Malaysia’s Hot Spring

2021 ◽  
Vol 12 (1S) ◽  
pp. 31-39
Author(s):  
Nur Dinie Zailan ◽  
Nurul Asma Hasliza Zulkifly ◽  
Afnani Alwi ◽  
Siti Noor Syuhada Muhammad Amin ◽  
Nadiawati Alias
Keyword(s):  
Nitrogen Source ◽  
Yeast Extract ◽  
Hot Springs ◽  
Hot Spring ◽  
Inositol Phosphates ◽  
Nitrogen Sources ◽  
Bacterial Strains ◽  
Phytase Production ◽  
Ph Values ◽  
Soybean Extract

Efficient strategies for phytase production gained increasing importance as more applications require high amounts of phytase for the market. Four phytase-producing bacterial strains isolated from Malaysia’s hot springs were used in this study to determine the effect of nitrogen sources on phytase production. All of the strains were screened out by applying halozone method which shows all of the strains were definitely positive phytase producer. Phytase Screening Medium (PSM) with soybean extract as substrate was used as a cultivation medium. Optimised condition with 1.0 % (w/v) of glucose (as carbon source), pH 5.5 and 37°C temperature was applied. Yeast extract and peptone were used to identify optimum nitrogen source in maximum phytase production. Quantitative analysis observed were optical density, colony forming unit, pH values and phytase activity to identify the effect of nitrogen source in phytase production. The finding was bacterial strain L3 as the best producer in producing maximum phytase (0.2162 U/mL) with optimised condition using yeast extract as nitrogen source. Findings in this study proved that yeast extract act as the optimum nitrogen source which contribute to maximum phytase production as supported by previous studies. This study can provide an efficient strategy to produce maximum phytase as few studies stated that phytase is an application tool in functional food production that consists of myo-inositol phosphates that is believed to have important pharmacological effects.

scholarly journals DETERMINATION OF OPTIMUM CULTURE CONDITIONS FOR MYCELIAL GROWTH OF Macrolepiota procera MUSHROOM

2020 ◽  
Vol 19 (1) ◽  
pp. 11-20
Author(s):  
Aysun Pekşen ◽  
Beyhan Kibar
Keyword(s):  
Yeast Extract ◽  
Mycelial Growth ◽  
Nitrogen Sources ◽  
Culture Conditions ◽  
Malt Extract ◽  
The Public ◽  
Carbon And Nitrogen ◽  
Ph Values ◽  
Incubation Temperatures ◽  
Macrolepiota Procera

Macrolepiota procera, commonly called the Parasol Mushroom, is a delicious mushroom collected from the nature and commonly consumed by the public in many regions of Turkey. This study was conducted to determine the optimum culture conditions (pH, temperature, carbon and nitrogen sources) for mycelial growth of M. procera. Three pH values (pH 5.0, 5.5 and 6.0), four incubation temperatures (15, 20, 25 and 30°C), seven carbon (C) sources (dextrose, glucose, lactose, maltose, mannitol, sucrose and xylose) and six nitrogen (N) sources ((NH4)2HPO4, NH4NO3 and Ca(NO3)2, malt extract, peptone and yeast extract) were investigated. In the second step of the study, the effect of seven pH values (4.0, 4.5, 5.0, 5.5, 6.0, 6.5 and 7.0) on the mycelial colony diameter was examined at 20 and 25°C since these temperatures gave the best mycelial growth in the previously conducted temperature experiment. The best mycelial growth was determined at pH 6.0. The optimum temperature for mycelial growth of M. procera was found as 25°C. The use of glucose as carbon source and yeast extract and peptone as nitrogen source in the culture medium gave the best results for mycelial growth. Determining of optimum culture conditions for mycelial growth of M. procera will provide important contributions to the fortcoming studies on it’s commercially cultivation in Turkey.

Anoxybacillus sp. AH1, an α-amylase-producing thermophilic bacterium isolated from Dargeçit hot spring

Biologia ◽  
2015 ◽  
Vol 70 (7) ◽  
Author(s):  
Ömer Acer ◽  
Hemşe Pırınççiğlu ◽  
Fatma Matpan Bekler ◽  
Reyhan Gül-Güven
Keyword(s):  
Hot Springs ◽  
Hot Spring ◽  
Thermophilic Bacteria ◽  
Potato Starch ◽  
Nitrogen Sources ◽  
Soluble Starch ◽  
Thermophilic Bacterium ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Optimum Growth Temperature

AbstractThe present study was conducted to isolate α-amylase-producing thermophilic bacteria from Darge¸cit hot springs in Turkey. The morphological, biochemical and physiological characterisation, as well as genetic analysis by 16S rRNA sequences indicated that the isolated strain AH1 was a member of Anoxybacillus genus. The strain was aerobe, Gram-positive and spore-forming rod, exhibiting optimum growth temperature and pH of 60ºC and 7.0-7.5, respectively. Optimization of growth medium and enzyme assay conditions for extracellular α-amylase production by the novel thermophilic Anoxybacillus sp. AH1 were carried out in many different media containing a variety of carbon and nitrogen sources. Among various carbon and nitrogen sources, peptone (2054.1 U/mL) at 1% and maltose (1862.9 U/mL) at 0.5% increased α-amylase activity, compared to controls. Moreover, a high enzyme production was observed with potato starch at 0.5% and 1% (2668.4 U/mL and 3627 U/mL, respectively), as well as with 1% soluble starch (2051.9 U/mL). The enzyme activity was found to be rather high in the presence of CaCl

scholarly journals Influence of Nitrogen Sources on D-Lactic Acid Biosynthesis by Sporolactobacillus laevolacticus DSM 442 Strain

Fermentation ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 78
Author(s):  
Alicja Katarzyna Michalczyk ◽  
Sylwia Garbaczewska ◽  
Bolesław Morytz ◽  
Arkadiusz Białek ◽  
Jerzy Zakrzewski
Keyword(s):  
Lactic Acid ◽  
Nitrogen Source ◽  
Yeast Extract ◽  
Optical Purity ◽  
Nitrogen Sources ◽  
Fermentation Medium ◽  
Agricultural Product ◽  
Acid Biosynthesis ◽  
Batch Cultures ◽  
Pea Seed

The purpose of this study was to explore the possibility of replacing an expensive yeast extract contained in the fermentation medium for D-lactic acid (D-LA, R-lactic acid) biosynthesis with an alternative nitrogen source. The screening studies were conducted under stationary conditions and showed that pea seed hydrolysate was the most beneficial substrate in the process of D-LA biosynthesis by the strain Sporolactobacillus laevolacticus DSM 442 among the used inorganic and organic nitrogen sources, waste materials, food and agricultural products. After 96 h, 75.5 g/L D-LA was obtained in batch cultures in a medium containing pea seed hydrolysate, with an average productivity of 0.79 g/L/h, yield of 75.5%, and optical purity of 99.4%. In batch cultures fed once, in a medium with an analogous composition, 122.6 g/L D-LA was obtained after 120 h, and the average yield, productivity and optical purity were 87.6%, 1.021 g/L/h, and 99.6%, respectively. Moreover, the amount of D-LA obtained in the fermentation medium enriched with the above-mentioned cheap agricultural product was similar to the amounts obtained in the medium containing yeast extract in both stationary and bioreactor cultures. Our research shows that hydrolyzed pea seeds, which belong to the legume family, may be a promising nitrogen source for the production of D-LA on an industrial scale.

scholarly journals Examing the aniline degradation by Pseudomonas moraviensis AN-5

2018 ◽  
Vol 1 (6) ◽  
pp. 32-39
Author(s):  
Duc Danh Ha
Keyword(s):  
Environmental Pollution ◽  
Nitrogen Source ◽  
Aromatic Compound ◽  
Yeast Extract ◽  
Aquatic Species ◽  
Bacterial Strains ◽  
Carbon And Nitrogen ◽  
Water Problems

Aniline is a toxic aromatic compound causing environmental pollution, especially water problems, which harmfully affect to human and aquatic species. Seven bacterial strains were isolated from the sewerage of biological and chemical laboratories using aniline as a sole carbon and nitrogen source. Among them, Pseudomonas moraviensis AN-5 showed the highest efficiency. The aniline-degrading P. moraviensis AN-5 utilized aniline completely after 3 days at concentrations ≤ 4.0 mM. The addition of yeast extract and succinate stimulated the growth and aniline degradation of the strain. The investigation of aniline degraration pathway of P. moraviensis AN-5 showed that aniline was degraded by AN-5 via the ortho-clevage pathway.

scholarly journals Effect of pH on Different Strains of Phytase Producing Bacteria from Malaysia’s Hot Spring

2020 ◽  
Vol 11 (1S) ◽  
Author(s):  
Ainin Sofiya Kholed ◽  
Nurul Asma Hasliza Zulkifly ◽  
Afnani Alwi@Ali ◽  
Tajul Afif Abdullah ◽  
Nadiawati Alias
Keyword(s):  
Culture Condition ◽  
Hot Spring ◽  
Feed Additives ◽  
Phytase Activity ◽  
Future Research ◽  
Bacillus Sp ◽  
Bacterial Strains ◽  
Phytase Production ◽  
Sodium Phytate ◽  
The Media

In the recent research, the optimisation of culture condition for phytase production rarely done for Acetinobacter baumanii. The optimisation of the phytase production from the bacterial strains largely contributed by Bacillus sp. The study on the phytase originated from hot spring are limited and the species that identified from the hot spring samples are not in the same species from the previous study and mainly the species isolated from Bacillus sp. In this study, four potential strains of bacteria producing phytase isolated from hot spring in several regions in Malaysia. For enrichment of the bacterial, Nutrient Agar was used, meanwhile for batch culture optimisation, the bacteria producing phytase grown in modified liquid Phytase Screening Media with soy extract as agro residual substrate as a replacement for sodium phytate, the chemical substrate. The bacteria were screened for their ability to produce clear zone in solid PSM with sodium phytate as substrate. Optimisation of media through its physical factor that is pH of the media carried out using shake flask scale in laboratory. The growth of the bacterial strains and phytase activity measured quantitatively through the two different pH of media at pH 5.5 and pH 7. The analysis of colony-forming unit and pH determination after fermentation was carried out in this study. From the study, bacterial strain L3 from Labis, Johor has the highest phytase activity in the two parameters studied where the inorganic phosphate released at pH 5.5 (0.21953 U/mL) and pH 7 (0.2047 U/mL). Optimisation carried out through manipulating the culture condition that is pH of the media to determine at which condition has the highest phytase production. Several effects on enzyme activity caused by culture conditions identified. The optimisation of the fermentation medium able to contribute to the less cost production of the industrial enzyme as phytase has potential production for feed additives for poultry feeding. In the future research, molecular identification of the bacterial strains from the better-quality bacteria producing phytase grown in optimised culture media to investigate the molecular identity of the bacterial.

scholarly journals Comparison on Effects of Temperature on Different Strains of Phytase Producing Bacteria Isolated from Malaysia’s Hot Spring

2020 ◽  
Vol 11 (1S) ◽  
Author(s):  
Anis Adilah Mustafa ◽  
Nurul Asma Hasliza Zulkifly ◽  
Afnani Alwi@Ali ◽  
Tajul Afif Abdullah ◽  
Nadiawati Alias
Keyword(s):  
Enzyme Activity ◽  
Hot Springs ◽  
Ph Value ◽  
Hot Spring ◽  
Culture Method ◽  
Phytase Activity ◽  
Bacterial Strains ◽  
Temperature Optimization ◽  
Biochemical Research ◽  
Different Strains

The main purpose of this research was to find the best growth curve for bacterial growth and the optimum temperature for the production of phytase from different potential phytase producing bacterial strains. A total of four strains used were originally isolated from hot springs in Malaysia, which were in Labis, Johor (L3), Dusun Tua, Selangor (RT), Ulu Legong, Kedah (A) and Ranau, Sabah (B9). Nutrient Agar (NA) and modified Phytase Screening Medium (PSM) liquid media were used for the culture enrichment while optimisation was carried out through batch culture method using a shake-flask scale. Strains growth and enzyme activity were quantitatively measured at different temperatures at (30°C and 37°C) values. Enzyme activity was determined according to the reaction of the phytase with its substrate (sodium phytate) and expressed in units of phytase activity (U/ mL). As for the overall, strain L3 (from Labis, Johor) exhibit promising rate of Pi released in the media at 30°C and 37°C, with optimum phytase activity values of 0.2047 U/mL and 0.2195 U/mL, respectively. The pH of the cultures was also measured, where it shows that strains grown in cultures at 37°C produced a higher phytase activity and resulting a lower reading of pH compared when grown at 30°C. All around, L3 strains has the lowest value of pH when cultured at 30°C and 37°C, with the pH value of 3.62 and 2.37, respectively.  From the result obtained, the lower pH indicates the process of phytic acid degradation take place by the phytase in producing inorganic phosphate (Pi) due to the accumulation of organic acid. Since these bacterial strains were originally taken from Hot Springs, further analysis of temperature optimization using 55°C and even 60°C should be carried out. In the future, biochemical research and molecular identification may also be carried out to identify molecular identity in the strains.

scholarly journals Effect of Carbon Sources on Different Strains of Phytase-Producing Bacteria Isolated from Malaysia’s Hot Spring

2021 ◽  
Vol 12 (2) ◽  
pp. 29-38
Author(s):  
Nurul Izyan Che Mohamood ◽  
Nadiawati Alias ◽  
Nurul Asma Hasliza Zulkifly
Keyword(s):  
Phytic Acid ◽  
Inorganic Phosphate ◽  
Hot Springs ◽  
Animal Feed ◽  
Hot Spring ◽  
Carbon Sources ◽  
Feed Additives ◽  
Phytase Activity ◽  
Phytase Production ◽  
Different Strains

Animal feed from cereal grains and oilseed meals mainly containing phytic acid which has adverse effects on animal nutrition and its environment. Ruminants can easily digest the phytic acid as they have fungi and bacteria in their guts which can produce phytase to degrade the phytic acid. Meanwhile, phytic acid in non-ruminant animals is poorly digested due to the lack of sufficient phytase in their guts. Thus, the feed must be supplemented with inorganic phosphate to ensure it can absorb adequate nutrients. This study aimed to determine the effects of using different carbon sources to the growth of different strains of phytase producing bacteria based on optical density (OD), colony forming unit (CFU), and their phytase production. All four strains of potentially producing-phytase bacteria  have been isolated from several hot springs in Malaysia. The bacteria were grown in modified Phytase Screening Medium (PSM) with glucose and lactose as a carbon source and under optimum culture conditions (pH 5.5, 37˚C, 200 rpm) for 72 hours. For quantitative screening of phytase production, the bacterial cultures were harvested to obtain the supernatants that were used to measure the amount of inorganic phosphorus released by the bacterial strains. Among these carbon sources, glucose has shown consistency between their CFU counts and the observed ODs whereas lactose shown inconsistency. Meanwhile, the maximum phytase activity was recorded for all strains in the presence of glucose in which bacteria strain L3 (0.0404 U/mL), RT (0.0359 U/mL), B9 (0.0262 U/mL), and A (0.0263 U/mL). As for the overall, strain L3 (Labis, Johor) gave a promising rate of inorganic phosphate released with optimum phytase activity value of 0.0404 U/mL in presence of glucose and lactose. The optimisation of the fermentation medium can contribute to more economical production of industrial enzyme as phytase has the potential to produce feed additives for poultry feeding.

Screening of Nitrogen Sources in the Medium for Streptococcus thermophilus Using Plackett–Burman Design

2012 ◽  
Vol 531 ◽  
pp. 532-535 ◽  
Author(s):  
He Chen ◽  
Chuan Na Li ◽  
Guo Wei Shu ◽  
Chang Feng Wang
Keyword(s):  
Nitrogen Source ◽  
Yeast Extract ◽  
Casein Hydrolysate ◽  
Nitrogen Sources ◽  
Streptococcus Thermophilus ◽  
Meat Extract ◽  
Viable Bacteria ◽  
Burman Design ◽  
Soya Peptone ◽  
Plackett Burman Design

This study demonstrates that the type of nitrogen sources has an important influence on the growth of Streptococcus thermophilus.at the same time ,viable counts was studied by in the medium containing various nitrogen sources (peptone, yeast extract, meat extract, tryptone，soya peptone and casein hydrolysate). The results indicated that soya peptone was the most efficient nitrogen source and and the influence of different concentrations of soya peptone on growth was determined. The results indicated that viable bacteria were stimulated by the high soya peptone concentration (30g/L).

scholarly journals OPTIMASI NUTRISI MEDIA PERTUMBUHAN BAKTERI TERMOFIL PENGHASIL SELULASE DARI SUMBER AIR PANAS RIMBO PANTI

2015 ◽  
Vol 5 (1) ◽  
pp. 1
Author(s):  
Armaini ◽  
Abdi Dharma ◽  
Sumaryati Syukur ◽  
Jamsari
Keyword(s):  
Nitrogen Source ◽  
Hot Springs ◽  
Inorganic Nitrogen ◽  
Carbon Sources ◽  
Cellulase Activity ◽  
Nitrogen Sources ◽  
Cellulase Production ◽  
Optimum Concentration ◽  
Crystalline Cellulose ◽  
Inorganic Nitrogen Source

 Optimization have been done on the media for the growth of the isolated thermophiles bacteria from hot springs Rimbo Panti, the nutrients comprising variety of carbon sources such as CMC (carboxymethyl cellulose), avicell (micro crystalline cellulose), and cellobiose, with a variety of sources organic nitrogen, peptone, extracts yeast, tryptone, and urea, as well as variations consist of inorganic nitrogen sources, KNO3, NaNO3, (NH4)2SO4, and (NH4)NO3. Determination of cellulase activity performed using DNS reagent (3,5-dinitro salicylic acid). Maximum cellulase production with high activity based on the results of this research, the best of carbon source is CMC with optimum concentration 0.125%, inorganic nitrogen source is peptone with the optimum concentration of 0.3 to 0.4% and the inorganic nitrogen source is (NH4)2SO4 with optimum concentration of 0.2 - 0.25%. Optimization of size of inoculums obtained the optimum amount of inoculums 2%. Keywords: Optimization, thermophiles bacteria, cellulose, carbon sources, nitrogen sources

scholarly journals EFFECT OF DIFFERENT CULTURE CONDITION ON ANTIOXIDANT SECONDARY METABOLITES FROM ENDOPHYTIC FUNGI ISOLATED FROM TURMERIC ROOT

2017 ◽  
Vol 22 (1) ◽  
pp. 31 ◽  
Author(s):  
Eris Septiana ◽  
Partomuan Simanjuntak
Keyword(s):  
Antioxidant Activity ◽  
Nitrogen Source ◽  
Yeast Extract ◽  
Carbon Sources ◽  
Basal Medium ◽  
Nitrogen Sources ◽  
Growth Conditions ◽  
Carbon And Nitrogen ◽  
Antioxidant Compound ◽  
Initial Ph

Antioxidant is an interesting topic due to their capability to inhibit free radical and prevent damage because of oxidative processes. Endophyt fungi is one of antioxidant compound resources in nature. The low yield to gain antioxidant compound from fungi challenges to look for the composition of media and optimalization of growth conditions. This research aimed to know the effect of medium condition in different carbon and nitrogen sources as well as initial pH towards antioxidant activity of endophyt fungi Bo.Ci.Cl.A3. Shaker fermentation was used on 120 rpm at room temperature for 14 days. The carbon sources were glucose, sucrose, and starch and nitrogen sources were NaNO3, NH4NO3, and yeast extract with initial pH at 5, 7, and 9. Ethyl acetate was used as extractor. The results showed that endophyt fungi can produce secondary metabolite as antioxidant at all variation of fermented media. The nitrogen source of yeast extract could increase antioxidant activity of endophyt fungi Bo.Ci.Cl.A3, while other sources such as nitrogen source, carbon sources, and different initial pH on the basal medium that were used did not give increasing antioxidant activity. The conclusion of this research was the substitution of nitrogen source with yeast extract (3 g/L) on the basal medium Czapek Dox’s Broth could increase antioxidant activity of endophyt fungi Bo.Ci.Cl.A3.

Sign in / Sign up

Export Citation Format

Share Document