Effect of Polyamines on Chilling Tolerance in Seed Imbibition and Seed Germination in Maize

The seeds of red clover are heteromorphic and two color morphs can be visually recognized, light purple and yellow, resulting from heterozygosity and recessive homozygosity at two loci. Here, we report the responses of seed imbibition, seed germination, and early seedling growth of the two morphs to distilled water, sodium chloride, and complete nutrient solution. The sensitivityof red clover seeds to treatments increased with the stage of development in what seems to be a cumulative process. No differences were found in seed imbibition between morphs or between treatments. In seedling growth, on the contrary, treatments were always effective, but differences between morphs were only observed in seeds that were treated with nutrient solution, whereas in the intermediate stage of seed germination, the effects by treatments were observed together with the appearance of differences between morphs in distilled water and in the treatment by sodium chloride solution. Simultaneously, the superior performance of the yellow morph that was found in germination, which appears to be a trait stable across cultivars of red clover seeds, turned into a superior performance of the light purple morph in seedling growth.

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Effect of FLOWERING LOCUS C on seed germination depends on dormancy

Functional Plant Biology ◽

10.1071/fp16368 ◽

2017 ◽

Vol 44 (5) ◽

pp. 493 ◽

Cited By ~ 10

Author(s):

Logan Blair ◽

Gabriela Auge ◽

Kathleen Donohue

Keyword(s):

Arabidopsis Thaliana ◽

Seed Germination ◽

Genetic Pathway ◽

Flowering Locus C ◽

Primary Dormancy ◽

Seed Imbibition ◽

Secondary Dormancy ◽

Germination Temperature ◽

Flowering Locus ◽

Dormancy Induction

FLOWERING LOCUS C (FLC) has a major regulatory role in the timing of flowering in Arabidopsis thaliana (L.) Heynh. and has more recently been shown to influence germination. Here, we investigated the conditions under which FLC influences germination, and demonstrated that its effect depends on the level of primary and secondary dormancy and the temperature of seed imbibition. We tested the germination response of genotypes with different degrees of FLC activity over the course of after-ripening and after secondary dormancy induction by hot stratification. Genotypes with high FLC-activity showed higher germination; this response was greatest when seeds exhibited primary dormancy or were induced into secondary dormancy by hot stratification. In this study, which used less dormant seeds, the effect of FLC was more evident at 22°C, the less permissive germination temperature, than at 10°C, in contrast to prior published results that used more dormant seeds. Thus, because effects of FLC variation depend on dormancy, and because the range of temperature that permits germination also depends on dormancy, the temperature at which FLC affects germination can also vary with dormancy. Finally, we document that the effect of FLC can depend on FRIGIDA and that FRIGIDA itself appears to influence germination. Thus, pleiotropy between germination and flowering pathways in A. thaliana extends beyond FLC and involves other genes in the FLC genetic pathway.

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Imbibition, Germination, and Early Seedling Growth Responses of Light Purple and Yellow Seeds of Red Clover to Distilled Water, Sodium Chloride, and Nutrient Solution

Sci ◽

10.3390/sci1010010.v1 ◽

2019 ◽

Vol 1 (1) ◽

pp. 10

Author(s):

Ana Sofia Costa ◽

Luís Silva Dias ◽

Alexandra Soveral Dias

Keyword(s):

Sodium Chloride ◽

Seed Germination ◽

Nutrient Solution ◽

Seedling Growth ◽

Red Clover ◽

Superior Performance ◽

Distilled Water ◽

Seed Imbibition ◽

Early Seedling Growth ◽

Early Seedling

The seeds of red clover are heteromorphic and two color morphs can be visually recognized, light purple and yellow, resulting from heterozygosity and recessive homozygosity at two loci. Here, we report the responses of seed imbibition, seed germination, and early seedling growth of the two morphs to distilled water, sodium chloride, and complete nutrient solution. The sensitivity of red clover seeds to treatments increased with the stage of development in what seems to be a cumulative process. No differences were found in seed imbibition between morphs or between treatments. In seedling growth, on the contrary, treatments were always effective, but differences between morphs were only observed in seeds that were treated with nutrient solution, whereas in the intermediate stage of seed germination, the effects by treatments were observed together with the appearance of differences between morphs in distilled water and in the treatment by sodium chloride solution. Simultaneously, the superior performance of the yellow morph that was found in germination, which appears to be a trait stable across cultivars of red clover seeds, turned into a superior performance of the light purple morph in seedling growth.

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Effects of brassinosteroids and gibberellin on water uptake and performance of soya bean seeds under different temperatures

Seed Science and Technology ◽

10.15258/sst.2021.49.2.06 ◽

2021 ◽

Vol 49 (2) ◽

pp. 141-157

Author(s):

Kantima Thongsri ◽

Kanokwan Teingtham ◽

Juangjun Duangpatra ◽

Jutamas Romkaew

Keyword(s):

Seed Germination ◽

Low Temperature ◽

Seed Treatment ◽

Soya Bean ◽

Bean Seed ◽

Negative Effects ◽

Seed Imbibition ◽

Different Temperatures ◽

Mean Germination Time ◽

And Performance

Low temperature has negative effects on seed performance and seedling growth in soya bean, reducing stand establishment. It was hypothesised, that seed treatment with plant hormones could improve seed imbibition and performance at low temperature. Therefore, the effects of 24-epibrassinolide (EBL) and gibberellic acid (GA3) on soya bean seed germination were studied. Seeds of 'CM60' and 'CM0701-24' were imbibed with 0.25 or 0.50 ppm EBL, 50 or 100 ppm GA3, or distilled water at 10, 15, 20 and 25°C. The seed imbibition patterns of the two soya bean varieties followed a similar trend. Low temperature delayed seed imbibition resulting in longer time to reach the end of phases 1 and 2 of seed germination as compared with high temperature. Soya bean seeds treated with 50 or 100 ppm GA3 showed increased germination index, shoot and root length and reduced mean germination time. Thus, 50-100 ppm GA3 could be used as a pre-soaking seed treatment to improve soya bean seed and seedling performance under low temperature.

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Quantification of storage proteins during seed imbibition of native species from the brazilian caatinga vegetation

Semina Ciências Agrárias ◽

10.5433/1679-0359.2016v37n4p1733 ◽

2016 ◽

Vol 37 (4) ◽

pp. 1733

Author(s):

Fabrício Francisco Santos da Silva ◽

Bárbara França Dantas

Keyword(s):

Seed Germination ◽

Native Species ◽

Storage Proteins ◽

Seed Storage ◽

Seed Storage Proteins ◽

Northeastern Brazil ◽

Blotting Paper ◽

Seed Imbibition ◽

Caatinga Vegetation ◽

Before And After

Poincianella pyramidalis (Fabaceae), Schinopsis brasiliensis (Anacardiaceae) and Sideroxylon obtusifolium (Sapotaceae) are native species of the Caatinga vegetation from northeastern Brazil and have both biological importance and potential economic uses. Little is known about the water uptake and degradation of storage proteins during seed germination of these species. The aim of this study was to evaluate the imbibition and quantify the amount of storage proteins during seed germination of P. pyramidalis, S. brasiliensis and S. obtusifolium. Two lots of S. obtusifolium seeds with different vigour were used. Four replicates of 20 seeds of P. pyramidalis, S. brasiliensis and S. obtusifolium, were sown onto gerboxes with blotting paper soaked in distilled water and incubated during 72, 200 and 624 hours. Before and after imbibition seeds were weighed and frozen at until the sequential extraction and analysis of the seed storage proteins. Based on our results, we conclude that seed germination of P. pyramidalis, S. brasiliensis and S. obtusifolium has a well-defined triphasic imbibition. All storage proteins content of P. pyramidalis and S. brasiliensis seeds degraded along with the seed imbibition. Likewise, the content of albumins, globulins and glutelins decreased as S. obtusifolium seeds absorbed water.

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Imbibition, Germination, and Early Seedling Growth Responses of Light Purple and Yellow Seeds of Red Clover to Distilled Water, Sodium Chloride, and Nutrient Solution

Sci ◽

10.3390/sci1010032 ◽

2019 ◽

Vol 1 (1) ◽

pp. 32

Author(s):

Costa ◽

Dias ◽

Dias

Keyword(s):

Sodium Chloride ◽

Seed Germination ◽

Nutrient Solution ◽

Seedling Growth ◽

Red Clover ◽

Superior Performance ◽

Distilled Water ◽

Seed Imbibition ◽

Early Seedling Growth ◽

Early Seedling

The seeds of red clover are heteromorphic and two color morphs can be visually recognized, light purple and yellow, resulting from heterozygosity and recessive homozygosity at two loci. Here, we report the responses of seed imbibition, seed germination, and early seedling growth of the two morphs to distilled water, sodium chloride, and complete nutrient solution. The sensitivity of red clover seeds to treatments increased with the stage of development in what seems to be a cumulative process. No differences were found in seed imbibition between morphs or between treatments. In seedling growth, on the contrary, treatments were always effective, but differences between morphs were only observed in seeds that were treated with nutrient solution, whereas in the intermediate stage of seed germination, the effects by treatments were observed together with the appearance of differences between morphs in distilled water and in the treatment by sodium chloride solution. Simultaneously, the superior performance of the yellow morph that was found in germination, which appears to be a trait stable across cultivars of red clover seeds, turned into a superior performance of the light purple morph in seedling growth.

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