scholarly journals A Genetic Evaluation System for New Zealand White Rabbit Germplasm Resources Based on SSR Markers

Animals ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1258
Author(s):  
Jiali Li ◽  
Bin Zhao ◽  
Yang Chen ◽  
Bohao Zhao ◽  
Naisu Yang ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
New Zealand ◽  
Evaluation System ◽  
Zealand White Rabbit ◽  
Genetic Evaluation ◽  
Germplasm Resources ◽  
Polymorphic Loci ◽  
Marker Combination ◽  
New Zealand White Rabbits ◽  
Two Generations

At present, there is an abundance of quality domestic rabbit breeds in China. However, due to the lack of technical standards for the genetic evaluation of rabbit germplasm resources, there have been a number of problems, such as poor breed conservation. By studying the genetic diversity of 130 New Zealand white rabbits (regardless of generation), we obtained the best simple sequence repeat (SSR) marker combination. We found that, when using microsatellite markers for the effective genetic evaluation of domestic rabbits, the number of records should be greater than 60 and the marker number more than 22. Through the comparative analysis of 30 combinations of 22 markers, the optimal combination of 22 markers was determined, and the 22 SSR polymorphic loci were distributed on different chromosomes. We performed a genetic analysis of 200 New Zealand white rabbits corresponding to two generations, using the best SSR polymorphic loci combination. There were no significant differences in the genetic diversity parameters between the two generations of rabbits (p > 0.05), indicating that the characteristics of this excellent rabbit germplasm have been effectively preserved. At the same time, we verified that the established method can be used to evaluate the breed conservation of rabbit germplasm resources.

scholarly journals An Investigation of the Relationship between Cyniclomyces guttulatus and Rabbit Diarrhoea

Pathogens ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 880
Author(s):  
Tuanyuan Shi ◽  
Xinlei Yan ◽  
Hongchao Sun ◽  
Yuan Fu ◽  
Lili Hao ◽  
...  
Keyword(s):  
New Zealand ◽  
Body Weight Gain ◽  
Animal Experiments ◽  
Zealand White Rabbit ◽  
Opportunistic Pathogen ◽  
Large Numbers ◽  
New Zealand White Rabbits ◽  
Positive Rate ◽  
Relationship Of ◽  
The Relationship

Cyniclomyces guttulatus is usually recognised as an inhabitant of the gastrointestinal (GI) tract in rabbits. However, large numbers of C. guttulatus are often detected in the faeces of diarrhoeic rabbits. The relationship of C. guttulatus with rabbit diarrhoea needs to be clearly identified. In this study, a C. guttulatus Zhejiang strain was isolated from a New Zealand White rabbit with severe diarrhoea and then inoculated into SPF New Zealand white rabbits alone or co-inoculated with Eimeriaintestinalis, another kind of pathogen in rabbits. Our results showed that the optimal culture medium pH and temperature for this yeast were pH 4.5 and 40–42 °C, respectively. The sequence lengths of the 18S and 26S ribosomal DNA fragments were 1559 bp and 632 bp, respectively, and showed 99.8% homology with the 18S ribosomal sequence of the NRRL Y-17561 isolate from dogs and 100% homology with the 26S ribosomal sequence of DPA-CGR1 and CGDPA-GP1 isolates from rabbits and guinea pigs, respectively. In animal experiments, the C. guttulatus Zhejiang strain was not pathogenic to healthy rabbits, even when 1 × 108 vegetative cells were used per rabbit. Surprisingly, rabbits inoculated with yeast showed a slightly better body weight gain and higher food intake. However, SPF rabbits co-inoculated with C. guttulatus and E. intestinalis developed more severe coccidiosis than rabbits inoculated with C. guttulatus or E. intestinalis alone. In addition, we surveyed the prevalence of C. guttulatus in rabbits and found that the positive rate was 83% in Zhejiang Province. In summary, the results indicated that C. guttulatus alone is not pathogenic to healthy rabbits, although might be an opportunistic pathogen when the digestive tract is damaged by other pathogens, such as coccidia.

scholarly journals The effect of age, anatomical site and bone structure on osteogenesis in New Zealand White rabbit

2015 ◽  
Vol 88 (2) ◽  
Author(s):  
Francesca Ravanetti ◽  
Edoardo Scarpa ◽  
Vittorio Farina ◽  
Marco Zedda ◽  
Carlo Galli ◽  
...  
Keyword(s):  
New Zealand ◽  
Bone Structure ◽  
Skeletal Maturity ◽  
Zealand White Rabbit ◽  
Anatomical Site ◽  
Bone Implants ◽  
New Zealand White Rabbits ◽  
Preclinical Trials ◽  
Parietal Bones

Among animal models, rabbits are widely used in medical research, as they fill the gap between smaller models, commonly employed in basic science, and larger ones, which are better suited for preclinical trials. Given their rapid growth, rabbits provide a valuable system for the evaluation of bone implants for tissue regeneration. By means of a histomorphometric analysis, here we quantified the mineral apposition rates (MARs) in osteonic, periosteum and endosteum osteogenic fronts, of skeletal elements within femur, tibia, radius, ulna, frontal and parietal bones in New Zealand White rabbits aged 6, 7 and 8 months. Our hypothesis is that the MAR varies according to the skeletal maturity of the animal, and also within the skeletal elements and the osteogenic fronts considered. In the present study we show that the MAR in both femur and tibia is significantly higher than in ulna and radius. We also demonstrate that the MAR in parietal bones is significantly higher compared to the MAR of both frontal and forelimb bones. Contrary to what was expected, the MARs of all the skeletal elements considered were not decreased following full skeletal maturity. Finally, the MAR of the osteonic-osteogenic front is the lowest in all of the skeletal elements considered. In conclusion, these results provide new important insights for the evaluation of bone implants, casting a light on the role of both age and osteogenic fronts on the bone MAR, and providing valuable information on the physiological bone turnover in New Zealand White rabbits.

scholarly journals Morphometry of megakaryocytes in the liver of New Zealand White rabbits during intrauterine and postnatal development

2002 ◽  
Vol 32 (6) ◽  
pp. 1011-1017
Author(s):  
Maria Rita Pacheco ◽  
Nilson Ferreira ◽  
Válder Rodrigues Melo ◽  
Silvana Martinez Baraldi-Artoni ◽  
Antonio Marcos Orsi ◽  
...  
Keyword(s):  
New Zealand ◽  
Shape Factor ◽  
Zealand White Rabbit ◽  
Postnatal Period ◽  
Maximum Diameter ◽  
Postnatal Life ◽  
Minimum Diameter ◽  
Intrauterine Life ◽  
New Zealand White Rabbit ◽  
New Zealand White Rabbits

The hepatic megakaryocytic cells of New Zealand White rabbit in the intrauterine phase and in the immediate postnatal period were studied. Statistical analysis of the data concerning the cytoplasm and nucleus of those cells, i.e., area, perimeter, maximum diameter, minimum diameter, volume and shape factor, presented significant differences (p<0.01) for F values concerning the life phases studied on15th, 22nd and 29th day of intrauterine life and 10th day of postnatal life, and for F values for animal within each phase. The Tukey’s test showed that most of the parameters studied in the cytoplasm and nucleus of these megakaryocytic cells presented the lowest values on the 15th day of intrauterine life and the highest on the 22nd day of the same phase.

scholarly journals Model of a critical size defect in the New Zealand White rabbit’s tibia

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
Che Nor Zarida Che Seman ◽  
Zamzuri Zakaria ◽  
Mohamed Azril Mohamed Amin ◽  
Mohd Shukrimi Awang ◽  
Nazri Mohd Yusof ◽  
...  
Keyword(s):  
New Zealand ◽  
Critical Size ◽  
Zealand White Rabbit ◽  
Control Group ◽  
Critical Size Defect ◽  
New Zealand White Rabbit ◽  
Surgical Suture ◽  
Study Results ◽  
Rabbit Tibia ◽  
New Zealand White Rabbits

Introduction: Critical size defects (CSD) in the long bones of New Zealand White rabbit (Oryctolagus cuniculus) have been used for years as an experimental model for investigation of the effectiveness of a new bone substitute materials. There are varieties of protocols available in the literature. This technical note attempts to present an alternative surgical technique of a CSD in the New Zealand white rabbit tibia. Methods: Thirty-nine New Zealand White rabbits were used in this study. A CSD of approximately 4.5 mm (width) X 9.0 mm (length) was surgically drilled at the proximal tibial metaphysis, approximately 1 cm from the knee joint. The surrounding of soft tissue was repositioned and sutured layer by layer with bio absorbable surgical suture. Povidone soaked gauze and bandage were used as a dressing. There is no control group used in this study. Results: This alternative method created good CSD with less bleeding from the muscle observed. No mortality on other surgical complications were observed within 6 weeks, 12 weeks and 24 weeks following surgery. Conclusions: A simple and safe method for performing critical size defect was demonstrated and recommended as an approach for surgery on New Zealand White rabbits.

scholarly journals Bacterial keratitis: similar bacterial and clinical outcomes in female versus male New Zealand White rabbits infected with Serratia marcescens

2021 ◽  
Author(s):  
Eric G Romanowski ◽  
Sanya Yadav ◽  
Nicholas A Stella ◽  
Kathleen A Yates ◽  
John E Romanowski ◽  
...  
Keyword(s):  
New Zealand ◽  
Serratia Marcescens ◽  
Viral Infections ◽  
Zealand White Rabbit ◽  
Gram Negative Bacteria ◽  
Bacterial Keratitis ◽  
Gram Negative ◽  
Single Sex ◽  
New Zealand White Rabbits ◽  
Microbiological Parameters

Females and males respond differently to a number of systemic viral infections. Differences between females and males with respect to the severity of keratitis caused by Gram-negative bacteria such as Serratia marcescens are less well established. In this study we injected female and male New Zealand White rabbit corneas with a keratitis isolate of S. marcescens and evaluated the eyes after 48 hours for a number of clinical and microbiological parameters. No statistical differences in bacterial burden and corneal scores were recorded between female and male rabbits although there was a non-significant trend toward a higher frequency of female rabbits demonstrating hypopyons. This data suggests that for experimental bacterial keratitis studies involving Gram-negative rods, a single sex or mixed group of rabbit is sufficient for evaluating pathology and bacterial burdens. This will reduce the number of animals used for subsequent studies.

scholarly journals Posterolateral Intertransverse Lumbar Arthrodesis in the New Zealand White Rabbit Model: The Illustration of an Alternative Surgical Approach

2020 ◽  
Vol 11 (2) ◽  
Author(s):  
Zunariah B ◽  
Zamzuri Z ◽  
Che Nor Zarida CS ◽  
Rosnani AJ
Keyword(s):  
New Zealand ◽  
Surgical Complication ◽  
Rabbit Model ◽  
Zealand White Rabbit ◽  
Paraspinal Muscles ◽  
New Zealand White Rabbit ◽  
Alternative Approach ◽  
New Zealand White Rabbits ◽  
Lumbar Arthrodesis ◽  
Good Exposure

Introduction: The New Zealand White (NZW) rabbit model is commonly used for the research of posterolateral intertransverse lumbar arthrodesis. The standard approach reported by many researchers are a muscle splitting approach through the intermuscular plane between multifidus and longissimus muscles. Methods: In this research the fusion bed was exposed by retracting the three groups of paraspinal muscles (multifidus, longissimus and iliocostalis) medially. Results: This alternative method showed good exposure of the transverse processes and intertransverse membrane with less bleeding from the muscles observed. No death due to surgical complication was observed out of twenty-four rabbits operated. Conclusion: An easy and safe method for performing posterolateral intertransverse lumbar arthrodesis was demonstrated and recommended as an alternative approach for surgery on New Zealand White rabbits.

scholarly journals Genetic diversity and population structure of Meretrix petechialis in China revealed by sequence-related amplified polymorphism markers

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8723 ◽  
Author(s):  
Qiaoyue Xu ◽  
Junhong Zheng ◽  
Hongtao Nie ◽  
Qingzhi Wang ◽  
Xiwu Yan
Keyword(s):  
Genetic Diversity ◽  
Genetic Differentiation ◽  
Genetic Distance ◽  
Gene Diversity ◽  
Scientific Basis ◽  
Germplasm Resources ◽  
Polymorphic Loci ◽  
Information Index ◽  
Geographical Populations ◽  
Meretrix Petechialis

Genetic variation in nine stocks of Meretrix petechialis collected from China was analyzed using sequence-related amplified polymorphism (SRAP) markers. Eight primer pairs produced 132 polymorphic loci with an average of 16.5 loci per primer pair. A population from Jiangsu had the highest percentage of polymorphic loci at 27.27%, suggesting that these resources had a rich genetic diversity. The Nei’s gene diversity of the nine populations ranged from 0.0647 to 0.0793; a population from Shandong was the lowest and a population from North Korea the highest. The Shannon’s information index was between 0.1023 and 0.1202, with the lowest in the Shandong population and the highest in the Jiangsu population. The Nei’s unbiased genetic distance between the nine populations was 0.0243–0.0570 and the genetic similarity was 0.9446–0.9760; the genetic distance between Guangxi and Shandong populations was the furthest (0.0570) and the genetic distance between Shandong and Jiangsu populations was the closest (0.0243). Nei’s gene diversity analysis indicated that the genetic variance was mainly found within individual geographical populations, and the analysis of molecular variance revealed low but significant genetic differentiation among local and regional populations. The limited gene flow (Nm = 0.555) was inferred as a major reason for the extent of genetic differentiation in M. petechialis. The results obtained here indicated that M. petechialis have high degree of genetic diversity and the potential of further breeding with excellent germplasm resources. This study provides a scientific basis for the protection of germplasm resources and the breeding of M. petechialis.

scholarly journals Model of A Critical Size Defect in the New Zealand White Rabbit’s Tibia

2020 ◽  
Vol 17 (1) ◽  
Author(s):  
Che Nor Zarida Che Seman ◽  
Zamzuri Zakaria ◽  
Mohd Ariff Sharifudin ◽  
Aminudin Che Ahmad ◽  
Mohd Shukrimi Awang ◽  
...  
Keyword(s):  
New Zealand ◽  
Critical Size ◽  
Zealand White Rabbit ◽  
Technical Note ◽  
Layer By Layer ◽  
X Rays ◽  
Critical Size Defects ◽  
Surgical Suture ◽  
Rabbit Tibia ◽  
New Zealand White Rabbits

Introduction: Critical size defects (CSD) in the long bones of New Zealand White rabbits (Oryctolagus cuniculus) have been used for years as an experimental model for investigation of the effectiveness of a new bone substitute material. There are varieties of protocols available in the literature. This technical note attempts to present an alternative surgical technique of a CSD in the New Zealand white rabbit tibia. Methods: Thirty-nine New Zealand White rabbits were used in this study. A CSD of approximately 4.5 mm (width) X 9.0 mm (length) was surgically drilled at the proximal tibial metaphysis, approximately 1 cm from the knee joint. The surrounding of soft tissue was repositioned and sutured layer by layer with bioabsorbable surgical suture. Two x-rays of anteroposterior and lateral were taken before assessed under computed tomography scan at 6, 12 and 24 weeks. Results: This alternative method created CSD with less bleeding from the muscle observed. No mortality or other surgical complications observed within 6 weeks, 12 weeks and 24 weeks following surgery. Conclusion: A simple and safe method for performing CSD was demonstrated and recommended as an alternative approach for surgery on New Zealand White rabbits.

scholarly journals Interstitial Adipocytes in the Beagle Dog and New Zealand White Rabbit Choroid Plexus

2019 ◽  
Vol 47 (4) ◽  
pp. 553-555 ◽  
Author(s):  
Daniel Rudmann ◽  
Curtis Colleton
Keyword(s):  
Skeletal Muscle ◽  
New Zealand ◽  
Choroid Plexus ◽  
Zealand White Rabbit ◽  
Preclinical Studies ◽  
Pathologic Process ◽  
Beagle Dog ◽  
New Zealand White Rabbit ◽  
New Zealand White Rabbits ◽  
Microscopic Findings

The choroid plexus (CP) produces cerebrospinal fluid and has epithelial, interstitial, and vascular compartments. The CP is a potential site of toxicity, and recognizing the normal microanatomy in different animal models is important for the pathologist. In preclinical studies with beagle dog and New Zealand white rabbits, we observed variable numbers of adipocytes in the CP interstitium of control and xenobiotic-treated animals. The adipocytes were unilocular and consistent morphologically with white adipose tissue. There was a striking variability in the number of adipocytes; however, the presence of adipocytes was not associated with other microscopic findings that would suggest a pathologic process. The morphology of adipocytes was reminiscent of what is observed normally in the interstitium of other tissues like skeletal muscle, bone marrow, and the subcutis. Therefore, we propose that the interstitial adipocytes not be recorded as a finding in preclinical studies unless the adipocytes are altered spontaneously (ie, lipoma) or after xenobiotic treatment.

Sign in / Sign up

Export Citation Format

Share Document