SiRNA-Mediated Flotillin-2 (Flot2) Downregulation Inhibits Cell Proliferation, Migration, and Invasion in Gastric Carcinoma Cells

Objective Sulforaphane, which exerts an effective anti-cancer ability, is a phytochemical converted from cruciferous plants. Here, we aimed to identify whether sulforaphane could suppress autophagy during the malignant progression of gastric carcinoma and to explore the underlying mechanisms. Methods SGC7901 cells were transfected with miR-4521 mimics, inhibitor, and pcDNA3.1- PIK3R3, and treated with sulforaphane or autophagy inhibitor. Cell proliferation, apoptosis, and miR-4521 or PIK3R3 expression were detected. Results MiR-4521 over-expression suppressed LC3-II/I ratio and Beclin-1 expression but induced p62 expression in SGC7901 cells. MiR-4521 also reduced gastric carcinoma cell proliferation and promoted apoptosis in vitro. In the mechanical observation, we identified that miR-4521 directly targeted PIK3R3 to repress its expression, and PIK3R3 up-regulation partly antagonized miR-4521-mediated autophagy, proliferation, and apoptosis in gastric carcinoma cells. In addition, sulforaphane exerted effective anti-cancer functions by repressing autophagy and growth in tumor cells at a concentration-dependent way. MiR-4521 inhibition or PIK3R3 over-expression weakened the anti-cancer functions of sulforaphane in gastric carcinoma cells. Conclusion Consequently, miR-4521 suppressed autophagy during the malignant progression of gastric carcinoma by targeting PIK3R3. Thus, miR-4521 may be applied as a therapeutic target for sulforaphane in gastric carcinoma.

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Lidocaine inhibits growth, migration and invasion of gastric carcinoma cells by up-regulation of miR-145

BMC Cancer ◽

10.1186/s12885-019-5431-9 ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 11

Author(s):

Hongyang Sui ◽

Anfeng Lou ◽

Zhisong Li ◽

Jianjun Yang

Keyword(s):

Gastric Carcinoma ◽

Carcinoma Cells ◽

Migration And Invasion

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Exosomal circ_0088300 Derived From Cancer-Associated Fibroblasts Acts as a miR-1305 Sponge and Promotes Gastric Carcinoma Cell Tumorigenesis

Frontiers in Cell and Developmental Biology ◽

10.3389/fcell.2021.676319 ◽

2021 ◽

Vol 9 ◽

Author(s):

Hao Shi ◽

Shan Huang ◽

Mingde Qin ◽

Xiaofeng Xue ◽

Xingpo Guo ◽

...

Keyword(s):

Cell Proliferation ◽

Gastric Carcinoma ◽

Carcinoma Cell ◽

Migration And Invasion ◽

Circular Rnas ◽

Cancer Associated Fibroblasts ◽

Regulatory Pathway ◽

Gastric Carcinoma Cell ◽

Cancer Associated Fibroblast ◽

Potential Biomarker

Cancer-associated fibroblast (CAF)-derived exosomes play a major role in gastric carcinoma (GC) tumorigenesis. However, the mechanism behind the activity of circular RNAs in CAF-derived exosomes in GC remains unclear. In the present study, we identified differentially expressed circ_0088300 in GC tissues and plasma exosomes. We found that CAFs delivered functional circ_0088300 to GC tumor cells via exosomes and promoted the proliferation, migration and invasion abilities of GC cells. Furthermore, we demonstrated that circ_0088300 packaging into exosomes was driven by KHDRBS3. In addition, we verified that circ_0088300 served as a sponge that directly targeted miR-1305 and promoted GC cell proliferation, migration and invasion. Finally, the JAK/STAT signaling pathway was found to be involved in the circ_0088300/miR-1305 axis, which accelerates GC tumorigenesis. In conclusion, our results indicated a previously unknown regulatory pathway in which exosomal circ_0088300 derived from CAFs acts as a sponge of miR-1305 and promotes GC cell proliferation, migration and invasion; these data identify a potential biomarker and novel therapeutic target for GC in the future.

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Leptomycin B inhibits the proliferation, migration, and invasion of cultured gastric carcinoma cells

Bioscience Biotechnology and Biochemistry ◽

10.1080/09168451.2019.1673148 ◽

2019 ◽

Vol 84 (2) ◽

pp. 290-296

Author(s):

Hepan Zhu ◽

Yi Yang ◽

Li Wang ◽

Xiaobin Xu ◽

Tingting Wang ◽

...

Keyword(s):

Gastric Carcinoma ◽

Carcinoma Cells ◽

Migration And Invasion ◽

Leptomycin B

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Solanine Attenuated Hepatocarcinoma Migration and Invasion Induced by Acetylcholine

Integrative Cancer Therapies ◽

10.1177/1534735420909895 ◽

2020 ◽

Vol 19 ◽

pp. 153473542090989

Author(s):

Liang-Tzung Lin ◽

Chen-Yen Choong ◽

Chen-Jei Tai

Keyword(s):

Cell Proliferation ◽

Carcinoma Cells ◽

Migration And Invasion ◽

Hep G2 ◽

Hep G2 Cells ◽

Mesenchymal Transition ◽

Cell Proliferation And Migration ◽

G2 Cells ◽

And Migration ◽

Proliferation And Migration

Aim: Evidence has provided an explanation of the correlation between the nervous system and the tumor microenvironment. Neurotransmitters may be involved in different aspects of cancer progression. The glycoalkaloid solanine has been reported to suppress neural signaling pathways and exists in numerous plants, including Solanum nigrum, which have been demonstrated to inhibit cancer cell proliferation. Methods: We evaluated the potentials of solanine on inhibiting acetylcholine-induced cell proliferation and migration in hepatocellular carcinoma cells. Results: The results indicated that solanine markedly attenuated cell proliferation and migration via inhibiting epithelial-mesenchymal transition and matrix metalloproteinases in acetylcholine-treated Hep G2 cells. In addition, exosomes derived from acetylcholine-treated Hep G2 cells were isolated, and solanine showed inhibiting effects of extrahepatic metastasis on blocking cell proliferation in exosome-treated A549 lung carcinoma cells through regulating microRNA-21 expression. Conclusion: Solanine has strong potential for application in integrative cancer therapy.

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VRK1 promotes proliferation, migration, and invasion of gastric carcinoma cells by activating β-catenin

Neoplasma ◽

10.4149/neo_2021_210304n278 ◽

2021 ◽

Author(s):

Li Wang ◽

Rujun Zhai ◽

Hong Shen ◽

Guodong Song ◽

Fangxin Wan ◽

...

Keyword(s):

Gastric Carcinoma ◽

Carcinoma Cells ◽

Migration And Invasion

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miR-486-3p Controls the Apoptosis of Endometrial Carcinoma Cells

Journal of Biomaterials and Tissue Engineering ◽

10.1166/jbt.2022.2985 ◽

2022 ◽

Vol 12 (5) ◽

pp. 1002-1007

Author(s):

Donghua Wang ◽

Xiaoli Liu ◽

Lirong Cao ◽

Shixiong Gong ◽

Yi He ◽

...

Keyword(s):

Cell Proliferation ◽

Cell Migration ◽

Endometrial Carcinoma ◽

Real Time ◽

Spectrophotometric Method ◽

Real Time Pcr ◽

Carcinoma Cells ◽

Migration And Invasion ◽

Invasive Capacity ◽

Ec Cells

Our study aimed to discuss the mechanism of miR-486-3p in controlling the apoptosis of endometrial carcinoma (EC) cells. EC cells were divided into NC group, miR-486-3p mimic and miR-486-3p inhibitor group followed by analysis of miR-486-3p level by Real-time PCR, cell proliferation by spectrophotometric method, apoptosis by FCM, cell migration and invasion by Transwell analysis. EC cells showed reduced miR-486-3p level. The EC malignant biological behaviors could be prompted through retraining miR-486-3p level with increased EC cell invasive capacity. DDR1 was a target of miR-486-3p. The variation of tumor activity could be regulated through controlling DDR1 expression. In conclusion, the apoptotic and invasive characteristic of EC cells are restrained after overexpression of miR-486-3p in EC cells through targeting DDR1, indicating that miR-486-3p could be considered to be one kind of brand-new target for the treatment of EC.

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Synthetic Isoliquiritigenin Inhibits Human Tongue Squamous Carcinoma Cells through Its Antioxidant Mechanism

Oxidative Medicine and Cellular Longevity ◽

10.1155/2017/1379430 ◽

2017 ◽

Vol 2017 ◽

pp. 1-11 ◽

Cited By ~ 8

Author(s):

Cuilan Hou ◽

Wenguang Li ◽

Zengyou Li ◽

Jing Gao ◽

Zhenjie Chen ◽

...

Keyword(s):

Cell Proliferation ◽

Fluorescence Microscopy ◽

Gelatin Zymography ◽

Squamous Carcinoma ◽

Carcinoma Cells ◽

Migration And Invasion ◽

Antioxidant Mechanism ◽

Squamous Carcinoma Cells ◽

Human Tongue ◽

Tongue Squamous Carcinoma

Isoliquiritigenin (ISL), a natural antioxidant, has antitumor activity in different types of cancer cells. However the antitumor effect of ISL on human tongue squamous carcinoma cells (TSCC) is not clear. Here we aimed to investigate the effects of synthetic isoliquiritigenin (S-ISL) on TSCC and elucidate the underlying mechanisms. S-ISL was synthesized and elucidated from its nuclear magnetic resonance spectrum and examined using high performance liquid chromatography. The effects of S-ISL on TSCC cells (Tca8113) were evaluated in relation to cell proliferation, apoptosis and adhesion, migration, and invasion using sulforhodamine B assay, fluorescence microscopy technique, flow cytometry (FCM) analysis, and Boyden chamber assay. The associated regulatory mechanisms were examined using FCM and fluorescence microscopy for intracellular reactive oxygen species (ROS) generation, Gelatin zymography assay for matrix metalloproteinase (MMP) activities, and Western blot for apoptosis regulatory proteins (Bcl-2 and Bax). Our data indicated that S-ISL inhibited Tca8113 cell proliferation, adhesion, migration, and invasion while promoting the cell apoptosis. Such effects were accompanied by downregulation of Bcl-2 and upregulation of Bax, reduction of MMP-2 and MMP-9 activities, and decreased ROS production. We conclude that S-ISL is a promising agent targeting TSCC through multiple anticancer effects, regulated by its antioxidant mechanism.

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Expression of heparanase regulates cell proliferation, apoptosis, migration and invasion in vitro in papillary thyroid carcinoma cells with multiple signaling pathways involved

10.21203/rs.2.13185/v1 ◽

2019 ◽

Author(s):

Chuanjia Yang ◽

Siyang Zhang ◽

Xiaoying Chang ◽

Yonglian Huang ◽

Dongxu Cui ◽

...

Keyword(s):

Cell Proliferation ◽

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Signaling Pathways ◽

Signaling Molecules ◽

Carcinoma Cells ◽

Biological Behavior ◽

Migration And Invasion ◽

Papillary Thyroid ◽

Multiple Signaling

Abstract Background Heparanase (HPSE) is an endo-β-D-glucuronidase, which is found overexpressed in various human cancers. The purpose of our work was to investigate the possible role of HPSE and the involved signaling molecules in the development of papillary thyroid carcinoma. Methods In this study, the expression plasmid of HPSE and RNA interference with shRNA specific for HPSE were used to elucidate the effects of HPSE on proliferation, apoptosis, migration and invasion in papillary thyroid carcinoma cells of B-CPAP. The probable downstream signaling molecules of HPSE were also explored. Results The results showed that upregulation of HPSE significantly promoted cell proliferation, migration and invasion of B-CPAP cells, and interfered with cell apoptosis. On the contrary, knockdown of HPSE exhibited the opposite effects. Compared with the parental cells, HPSE silencing cells showed attenuated capabilities of proliferation, migration and invasion, yet the apoptotic rate of transfected cells was increased. The activations of various signaling molecules correlated with cell biological behavior were found to be regulated by HPSE upregulation or knockdown. Conclusions Our results suggested that HPSE probably contributed to the progression and metastasis of papillary thyroid carcinoma, which were associated with multiple signaling pathways. HPSE could be a potent molecular target for the therapeutic strategy of papillary thyroid carcinoma.

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