Protective Role of Poria Cocos Polysaccharide Induced Differentiation of Bone Marrow Mesenchymal Stem Cells in Chronic Kidney Disease

2021 ◽  
Vol 20 (1) ◽  
pp. 177-184
Author(s):  
Yanping Fu ◽  
Daliang Yu ◽  
Xi Xie ◽  
Yu Huang ◽  
Shuhui Li
Keyword(s):  
Therapeutic Strategy ◽  
Protective Role ◽  
Poria Cocos ◽  
Traditional Chinese Herbal Medicine ◽  
Chinese Herbal ◽  
Renal Morphology ◽  
Proliferation And Differentiation ◽  
Marrow Mesenchymal Stem Cells ◽  
Renal Regeneration

The progressive loss of renal function and accumulation of collagen leads to CKD. Human BM-MSCs are considered as an ideal therapeutic strategy for renal regeneration in the CKD. Polysaccharides extracted from Poria cocos, an edible medicinal mushroom, have been in use in the traditional Chinese herbal medicine as they exhibit antidiabetic, antioxidative, antitumor, and other pharmacological effects. Whether the polysaccharides of P. cocos could ameliorate the CKD via induction of BM-MSC differentiation remains to be explored. The data presented here show that the polysaccharides of P. cocos not only induced BM-MSC proliferation and differentiation, but also reduced the levels of proinflammatory cytokines and improved renal morphology.

Role of miR-214 in biomaterial transplantation therapy for osteonecrosis

2021 ◽  
pp. 1-13
Author(s):  
Yuying Wang ◽  
Rui He ◽  
Anqi Yang ◽  
Rui Guo ◽  
Jie Liu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Formation ◽  
Bone Scaffold ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Marrow Mesenchymal Stem Cells ◽  
Transplantation Therapy ◽  
Cells Cultured ◽  
In Cells

BACKGROUND: The effectiveness and availability of conservative therapies for osteonecrosis of the femoral head (ONFH) are limited. Transplantation of bone marrow mesenchymal stem cells (BMSCs) combined with Bio-Oss, which is a good bone scaffold biomaterial for cell proliferation and differentiation, is a new potential therapy. Of note, the expression of miRNAs was significantly modified in cells cultured with Bio-Oss, and MiR-214 was correlated positively with osteonecrosis. Furthermore, miR-214 was upregulated in cells exposed to Bio-Oss. OBJECTIVE: To investigate whether targeting miR-214 further improves the transplantation effect. METHODS: We treated BMSCs with agomiR-214 (a miR-214 agonist), antagomiR-214 (a miR-214 inhibitor), or vehicle, followed by their transplantation into ONFH model rats. RESULTS: Histological and histomorphometric data showed that bone formation was significantly increased in the experimental groups (Bio-Oss and BMSCs treated with antagomiR-214) compared with other groups. CONCLUSIONS: miR-214 participates in the inhibition of osteoblastic bone formation, and the inhibition of miR-214 to bone formation during transplantation therapy with Bio-Oss combined with BMSCs for ONFH.

scholarly journals Lead toxicity induces autophagy to protect against cell death through mTORC1 pathway in cardiofibroblasts

2015 ◽  
Vol 35 (2) ◽  
Author(s):  
Li Sui ◽  
Rui-Hong Zhang ◽  
Ping Zhang ◽  
Ke-Li Yun ◽  
Hong-Cai Zhang ◽  
...  
Keyword(s):  
Cell Death ◽  
Side Effects ◽  
Therapeutic Strategy ◽  
Lead Toxicity ◽  
Protective Role ◽  
Molecular Processes ◽  
Effective Therapeutic Strategy ◽  
Mtorc1 Pathway ◽  
Set Up

Lead is a metal with many recognized adverse health side effects, and yet the molecular processes in cardiofibroblasts underlying lead toxicity are still poorly understood. Our current findings will help to understand the role of lead-mediated toxicity in cardiofibroblasts, indicating that autophagy serves a protective role in response to ER stress, which affords to set up an effective therapeutic strategy for the numerous diseases related to lead-toxicity.

scholarly journals Protective Role of an Initial Low-Dose Septic Challenge against Lethal Sepsis in Neonatal Mice: A Pilot Study

2021 ◽  
Vol 10 (24) ◽  
pp. 5823
Author(s):  
Ruka Nakasone ◽  
Mariko Ashina ◽  
Takumi Kido ◽  
Harunori Miyauchi ◽  
Masafumi Saito ◽  
...  
Keyword(s):  
Neonatal Sepsis ◽  
Therapeutic Strategy ◽  
Low Dose ◽  
Inflammatory Responses ◽  
Lethal Dose ◽  
Protective Role ◽  
Bacterial Invasion ◽  
Initial Exposure ◽  
Lethal Sepsis

Neonatal sepsis is characterized by systemic bacterial invasion followed by a massive inflammatory response. At present, no therapeutic strategy has been found that significantly reduces the mortality of neonatal sepsis. We aimed to investigate the protective role of an initial low-dose septic challenge for the prevention of subsequent lethal sepsis in a mouse model. A stock cecal slurry (CS) solution was prepared from adult ceca. The LD83 (1.5 mg CS/g) was used for all animals. An initial challenge of normal saline (NS) or 0.5 mg CS/g (non-lethal dose) was administered at four days of age, then 1.5 mg CS/g was administered intraperitoneally at seven days of age (72 h post-initial challenge), and survival was monitored. Initial exposure to NS (n = 10) resulted in 90% mortality following exposure to the LD83 CS dose in contrast to an initial exposure to CS (n = 16), which significantly decreased mortality to 6% (p < 0.0001), reduced blood bacterial counts, attenuated inflammatory responses, and suppressed lipid mediators. Initial exposure to a non-lethal CS dose prior to exposure to a lethal CS dose significantly reduces sepsis mortality, a protective effect that might be mediated by modulating abnormal systemic inflammatory responses.

The Role of MiR-339 in the Teriparatide-Induced Proliferation and Differentiation of Bone Marrow Mesenchymal Stem Cells

2019 ◽  
Vol 9 (8) ◽  
pp. 1148-1153
Author(s):  
Hongsong Fang ◽  
Shuang Deng ◽  
Zhihui Jin ◽  
Hao Peng ◽  
Sen Chen
Keyword(s):  
Bone Marrow ◽  
Protein Level ◽  
Luciferase Activity ◽  
Cell Number ◽  
Concentration Cell ◽  
Transfected Cells ◽  
Alp Activity ◽  
Proliferation And Differentiation ◽  
Marrow Mesenchymal Stem Cells

Osteoporosis is closely related to BMMSCs differentiation. Teriparatide promotes BMMSCs proliferation and differentiation with unclear mechanism. Both miR-339 and DLX5 are closely related to the differentiation of BMMSCs. Our study intends to assess the mechanism by how teripeptide promotes the proliferation and differentiation of BMMSCs. Rat BMMSCs were cultured and transfected with miR-339 inhibitor/NC and then treated with tripeptide (0, 10, 20, 50 nmol/L) followed by analysis of cell proliferation by CCK8 assay, alkaline phosphatase (ALP) activity, expression of miR-339, DLX5, Runx2 and OCN by real-time PCR, and DLX5 protein level by Western blot. miR-339 inhibitor transfection significantly decreased miR-339 expression, increased DLX-5 protein level, cell number, ALP activity and expression of osteogenic genes. Compared with 0 nmol/L group, 10, 20, 50 nmol/L group presented significantly increased cell number. With increased teriparatide concentration, cell number, ALP activity and expression levels of Runx2 and OCN was increased gradually and miR-339 and DLX5 expression was reduced. The luciferase activity in miR339 inhibitor and pmirGLO-DLX5-3′ UTR-wt-transfected cells was higher than cells transfected with miR-339 NC and pmirGLO-DLX5-3′ UTR-wt. Teriparatide promotes BMMSCs osteogenic differentiation by down-regulating miR-339 which targets DLX5 expression.

scholarly journals Traditional Chinese Herbal Medicine Plays a Role in the Liver, Kidney, and Intestine to Ameliorate Hyperuricemia according to Experimental Studies

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Li Xu ◽  
Li Li Lu ◽  
Jian Dong Gao
Keyword(s):  
Herbal Medicine ◽  
Metabolic Disorders ◽  
Experimental Studies ◽  
Herbal Medicines ◽  
Traditional Chinese Herbal Medicine ◽  
Chinese Herbal ◽  
Stage Renal Disease ◽  
End Stage ◽  
Traditional Chinese Herbal Medicines

In the last few decades, hyperuricemia has drawn increasing attention owing to its global prevalence. Observational surveys have manifested that there is a relation between hyperuricemia and increased risks of hypertension, chronic kidney disease, cardiovascular events, metabolic disorders, end stage renal disease, and mortality. As alternatives, Traditional Chinese medicinal herbs have demonstrated concrete effects in mitigating hyperuricemia in different experiments. Researchers have made efforts to investigate the role of herbal medicine in attenuating hyperuricemia. This review focuses on traditional Chinese herbal medicines that have been reported to ameliorate hyperuricemia in experimental studies.

scholarly journals The Protective Role of Klotho in CKD-Associated Cardiovascular Disease

2020 ◽  
Vol 6 (6) ◽  
pp. 395-406
Author(s):  
Xianjin Bi ◽  
Ke Yang ◽  
Bo Zhang ◽  
Jinghong Zhao
Keyword(s):  
Cardiac Dysfunction ◽  
Therapeutic Strategy ◽  
Predictive Biomarker ◽  
Protective Role ◽  
Kidney Dysfunction ◽  
Accelerated Atherosclerosis ◽  
Progressive Development ◽  
Stage 1 ◽  
Overall Mortality

<b><i>Background:</i></b> Cardiovascular diseases (CVDs) are the leading cause of morbidity and mortality in advanced CKD. The major pathological changes of CKD-associated CVD are severe vascular media calcification, aberrant cardiac remodeling such as hypertrophy and fibrosis, as well as accelerated atherosclerosis. α-Klotho is proposed as an anti-aging gene, which is primarily expressed in the kidney. Recent studies reveal that α-Klotho deficiency is associated with profound cardiovascular dysfunction. Of note, CKD represents extremely declined α-Klotho levels, hinting that α-Klotho deficiency may be implicated in the pathogenesis of CKD-associated CVD. <b><i>Summary:</i></b> Based on the pathogenic mechanism of α-Klotho deficiency and decreased Klotho levels in the circulation even early in stage 1 of CKD, α-Klotho serves as a sensitive biomarker for renal insufficiency and also a novel predictor of risk of overall mortality of CVD events in CKD. Meanwhile, loss of Klotho resulted from kidney dysfunction markedly contributes to the progressive development of CKD and CVD. By contrast, prevention of Klotho decline using exogenous supplementation or genetically activated ways by several mechanisms can dramatically mitigate cardiac dysfunction, prevent vascular calcification, and retard the progression of CKD-accelerated atherosclerosis. <b><i>Key Messages:</i></b> Klotho deficiency is proposed as a novel predictive biomarker as well as a pathogenic contributor to CVD events in CKD. In the future, Klotho may be a crucial potential therapeutic strategy to decrease the burden of CVD comorbidity with CKD in clinics.

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