Role of miR-214 in biomaterial transplantation therapy for osteonecrosis

2021 ◽  
pp. 1-13
Author(s):  
Yuying Wang ◽  
Rui He ◽  
Anqi Yang ◽  
Rui Guo ◽  
Jie Liu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Formation ◽  
Bone Scaffold ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Marrow Mesenchymal Stem Cells ◽  
Transplantation Therapy ◽  
Cells Cultured ◽  
In Cells

BACKGROUND: The effectiveness and availability of conservative therapies for osteonecrosis of the femoral head (ONFH) are limited. Transplantation of bone marrow mesenchymal stem cells (BMSCs) combined with Bio-Oss, which is a good bone scaffold biomaterial for cell proliferation and differentiation, is a new potential therapy. Of note, the expression of miRNAs was significantly modified in cells cultured with Bio-Oss, and MiR-214 was correlated positively with osteonecrosis. Furthermore, miR-214 was upregulated in cells exposed to Bio-Oss. OBJECTIVE: To investigate whether targeting miR-214 further improves the transplantation effect. METHODS: We treated BMSCs with agomiR-214 (a miR-214 agonist), antagomiR-214 (a miR-214 inhibitor), or vehicle, followed by their transplantation into ONFH model rats. RESULTS: Histological and histomorphometric data showed that bone formation was significantly increased in the experimental groups (Bio-Oss and BMSCs treated with antagomiR-214) compared with other groups. CONCLUSIONS: miR-214 participates in the inhibition of osteoblastic bone formation, and the inhibition of miR-214 to bone formation during transplantation therapy with Bio-Oss combined with BMSCs for ONFH.

scholarly journals Bone Marrow Mesenchymal Stem Cells Stimulate Cardiac Stem Cell Proliferation and Differentiation

2010 ◽  
Vol 107 (7) ◽  
pp. 913-922 ◽  
Author(s):  
Konstantinos E. Hatzistergos ◽  
Henry Quevedo ◽  
Behzad N. Oskouei ◽  
Qinghua Hu ◽  
Gary S. Feigenbaum ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Cell Proliferation ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Cardiac Stem Cell ◽  
Stem Cell Proliferation ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Marrow Mesenchymal Stem Cells

Effects of Silver Nanoparticles on the In Vitro Culture and Differentiation of Human Bone Marrow-Derived Mesenchymal Cells

2016 ◽  
Vol 852 ◽  
pp. 1307-1312
Author(s):  
Xiao Qian Yu ◽  
Li Xin Xu
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Silver Nanoparticles ◽  
Antibacterial Properties ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Marrow Mesenchymal Stem Cells

The antibacterial properties of silver nanoparticles may be able to prevent inflammation around an implant when placed on the surface of the dental neck. The purpose of this study was to culture silver nanoparticles on human bone marrow stem cells in vitro to determine their effects on cell differentiation and to assess their biocompatibility. Silver nanoparticles were deposited on titanium foil implant surfaces using ion sputtering, and adult bone marrow mesenchymal stem cells were cultured on those surfaces to determine the effect of the silver nanoparticles on cell viability, suitability for subculture, proliferation and differentiation. The results indicated that the silver nanoparticles were biocompatible, allowing cell proliferation and differentiation of osteoblasts, but interfered with differentiation into chondrocytes and adipocytes.

The Role of MiR-339 in the Teriparatide-Induced Proliferation and Differentiation of Bone Marrow Mesenchymal Stem Cells

2019 ◽  
Vol 9 (8) ◽  
pp. 1148-1153
Author(s):  
Hongsong Fang ◽  
Shuang Deng ◽  
Zhihui Jin ◽  
Hao Peng ◽  
Sen Chen
Keyword(s):  
Bone Marrow ◽  
Protein Level ◽  
Luciferase Activity ◽  
Cell Number ◽  
Concentration Cell ◽  
Transfected Cells ◽  
Alp Activity ◽  
Proliferation And Differentiation ◽  
Marrow Mesenchymal Stem Cells

Osteoporosis is closely related to BMMSCs differentiation. Teriparatide promotes BMMSCs proliferation and differentiation with unclear mechanism. Both miR-339 and DLX5 are closely related to the differentiation of BMMSCs. Our study intends to assess the mechanism by how teripeptide promotes the proliferation and differentiation of BMMSCs. Rat BMMSCs were cultured and transfected with miR-339 inhibitor/NC and then treated with tripeptide (0, 10, 20, 50 nmol/L) followed by analysis of cell proliferation by CCK8 assay, alkaline phosphatase (ALP) activity, expression of miR-339, DLX5, Runx2 and OCN by real-time PCR, and DLX5 protein level by Western blot. miR-339 inhibitor transfection significantly decreased miR-339 expression, increased DLX-5 protein level, cell number, ALP activity and expression of osteogenic genes. Compared with 0 nmol/L group, 10, 20, 50 nmol/L group presented significantly increased cell number. With increased teriparatide concentration, cell number, ALP activity and expression levels of Runx2 and OCN was increased gradually and miR-339 and DLX5 expression was reduced. The luciferase activity in miR339 inhibitor and pmirGLO-DLX5-3′ UTR-wt-transfected cells was higher than cells transfected with miR-339 NC and pmirGLO-DLX5-3′ UTR-wt. Teriparatide promotes BMMSCs osteogenic differentiation by down-regulating miR-339 which targets DLX5 expression.

Role of melatonin in regulating neurogenesis: Implications for the neurodegenerative pathology and analogous therapeutics for Alzheimer’s disease

2020 ◽  
Vol 3 (2) ◽  
pp. 216-242 ◽  
Author(s):  
Mayuri Shukla ◽  
Areechun Sotthibundhu ◽  
Piyarat Govitrapong
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Adult Neurogenesis ◽  
Adult Brain ◽  
Therapeutic Approaches ◽  
Therapeutic Implications ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Progenitor Cell Proliferation

The revelation of adult brain exhibiting neurogenesis has established that the brain possesses great plasticity and that neurons could be spawned in the neurogenic zones where hippocampal adult neurogenesis attributes to learning and memory processes. With strong implications in brain functional homeostasis, aging and cognition, various aspects of adult neurogenesis reveal exuberant mechanistic associations thereby further aiding in facilitating the therapeutic approaches regarding the development of neurodegenerative processes in Alzheimer’s Disease (AD). Impaired neurogenesis has been significantly evident in AD with compromised hippocampal function and cognitive deficits. Melatonin the pineal indolamine augments neurogenesis and has been linked to AD development as its levels are compromised with disease progression. Here, in this review, we discuss and appraise the mechanisms via which melatonin regulates neurogenesis in pathophysiological conditions which would unravel the molecular basis in such conditions and its role in endogenous brain repair. Also, its components as key regulators of neural stem and progenitor cell proliferation and differentiation in the embryonic and adult brain would aid in accentuating the therapeutic implications of this indoleamine in line of prevention and treatment of AD.   

scholarly journals The effects of locomotion on bone marrow mesenchymal stem cell fate: insight into mechanical regulation and bone formation

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yuanxiu Sun ◽  
Yu Yuan ◽  
Wei Wu ◽  
Le Lei ◽  
Lingli Zhang
Keyword(s):  
Bone Marrow ◽  
Cell Fate ◽  
Differentiation Potential ◽  
Stem Cell Fate ◽  
Proliferation And Differentiation ◽  
Marrow Mesenchymal Stem Cells ◽  
Adipocytic Differentiation ◽  
Regulatory Effects ◽  
Insight Into ◽  
Mechanical Regulation

AbstractBone marrow mesenchymal stem cells (BMSCs) refer to a heterogeneous population of cells with the capacity for self-renewal. BMSCs have multi-directional differentiation potential and can differentiate into chondrocytes, osteoblasts, and adipocytes under specific microenvironment or mechanical regulation. The activities of BMSCs are closely related to bone quality. Previous studies have shown that BMSCs and their lineage-differentiated progeny (for example, osteoblasts), and osteocytes are mechanosensitive in bone. Thus, a goal of this review is to discuss how these ubiquious signals arising from mechanical stimulation are perceived by BMSCs and then how the cells respond to them. Studies in recent years reported a significant effect of locomotion on the migration, proliferation and differentiation of BMSCs, thus, contributing to our bone mass. This regulation is realized by the various intersecting signaling pathways including RhoA/Rock, IFG, BMP and Wnt signalling. The mechanoresponse of BMSCs also provides guidance for maintaining bone health by taking appropriate exercises. This review will summarize the regulatory effects of locomotion/mechanical loading on BMSCs activities. Besides, a number of signalling pathways govern MSC fate towards osteogenic or adipocytic differentiation will be discussed. The understanding of mechanoresponse of BMSCs makes the foundation for translational medicine.

An oligomer complementary to c-myc mRNA inhibits proliferation of HL-60 promyelocytic cells and induces differentiation

1988 ◽  
Vol 8 (2) ◽  
pp. 963-973
Author(s):  
J T Holt ◽  
R L Redner ◽  
A W Nienhuis
Keyword(s):  
Cell Growth ◽  
Protein Concentration ◽  
Myeloid Differentiation ◽  
Sequence Specificity ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Steady State Levels ◽  
Cell Growth And Differentiation ◽  
Antisense Oligomer

To study the role of a nuclear proto-oncogene in the regulation of cell growth and differentiation, we inhibited HL-60 c-myc expression with a complementary antisense oligomer. This oligomer was stable in culture and entered cells, forming an intracellular duplex. Incubation of cells with the anti-myc oligomer decreased the steady-state levels of c-myc protein by 50 to 80%, whereas a control oligomer did not significantly affect the c-myc protein concentration. Direct inhibition of c-myc expression with the anti-myc oligomer was associated with a decreased cell growth rate and an induction of myeloid differentiation. Related antisense oligomers with 2- to 12-base-pair mismatches with c-myc mRNA did not influence HL-60 cells. Thus, the effects of the antisense oligomer exhibited sequence specificity, and furthermore, these effects could be reversed by hybridization competition with another complementary oligomer. Antisense inhibition of a nuclear proto-oncogene apparently bypasses cell surface events in affecting cell proliferation and differentiation.

scholarly journals Updates in the pathophysiological mechanisms of Parkinson’s disease: Emerging role of bone marrow mesenchymal stem cells

2016 ◽  
Vol 8 (3) ◽  
pp. 106 ◽  
Author(s):  
Hanaa H Ahmed ◽  
Ahmed M Salem ◽  
Hazem M Atta ◽  
Emad F Eskandar ◽  
Abdel Razik H Farrag ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Stem Cells ◽  
Bone Marrow ◽  
Parkinson's Disease ◽  
Mesenchymal Stem Cells ◽  
Pathophysiological Mechanisms ◽  
Marrow Mesenchymal Stem Cells
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