The Antioxidant Effect of PLGA Nanoparticles Encapsulated with Lutein in Rats Treated with Hypercaloric Diet

Poly lactic-co-glycolic acid (PLGA) is a biodegradable polymer that has been the focus of intense research due to its potential applications in medical research. Its uses in nanotechnology are underlined by its capability of targeting various cells and delivering active compounds to different human tissues. To date, PLGA nanoparticles are employed in areas such as vaccination therapy, diagnostic imaging procedure and various applications in various anticancer therapies. The aim of the present study is to measure and evaluate different correlations between oxidative stress parameters in spleen and liver following administration of nanoparticles encapsulated with lutein in an animal model. Our results show that acute oral administration of PLGA NPs induces a change in the oxidative stress status in both liver and spleen of rats, but does not induce oxidative stress damage to cell structures such as lipids or proteins.

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Oxidative Stress Following PLGA Nanoparticles Administration to an Animal Model

Materiale Plastice ◽

10.37358/mp.17.2.4826 ◽

2017 ◽

Vol 54 (2) ◽

pp. 249-252 ◽

Cited By ~ 1

Author(s):

Daniela Miricescu ◽

Iulia Stanescu ◽

Paula Perlea ◽

Bogdan Calenic ◽

Radu Radulescu ◽

...

Keyword(s):

Oxidative Stress ◽

Animal Model ◽

Plga Nanoparticles ◽

Engineered Nanoparticles ◽

Sustained Delivery ◽

Imaging Procedures ◽

Cell Structures ◽

Stress Damage ◽

Substantial Interest ◽

Sustained Delivery Of Drugs

In the recent years, engineered nanoparticles (NPs) such as PLGA or poly-lactic-co-glycolic acid, have raised a substantial interest due to their possible medical applications in vaccination, diagnostic imaging procedures, cancer therapy or sustained delivery of drugs. The main aim of the present work is to evaluate key oxidative stress parameters in several organs following NPs administration in an animal model. Our data shows that acute oral administration of PLGA NPs induces a change in the antioxidant status in both rat liver and spleen, but may not induce oxidative stress damage to cell structures such as lipid or protein oxidation.

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The antioxidant effect of β-Glucan on oxidative stress status in experimental spinal cord injury in rats

Neurosurgical Review ◽

10.1007/s10143-005-0389-2 ◽

2005 ◽

Vol 28 (4) ◽

pp. 298-302 ◽

Cited By ~ 32

Author(s):

Hakan Kayali ◽

M. Fatih Ozdag ◽

Serdar Kahraman ◽

Ahmet Aydin ◽

Engin Gonul ◽

...

Keyword(s):

Oxidative Stress ◽

Spinal Cord ◽

Spinal Cord Injury ◽

Antioxidant Effect ◽

Experimental Spinal Cord Injury ◽

Cord Injury ◽

Oxidative Stress Status

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Protective Effects of Carvacrol against Oxidative Stress Induced by Chronic Stress in Rat’s Brain, Liver, and Kidney

Biochemistry Research International ◽

10.1155/2016/2645237 ◽

2016 ◽

Vol 2016 ◽

pp. 1-7 ◽

Cited By ~ 41

Author(s):

Saeed Samarghandian ◽

Tahereh Farkhondeh ◽

Fariborz Samini ◽

Abasalt Borji

Keyword(s):

Oxidative Stress ◽

Restraint Stress ◽

Reduced Glutathione ◽

Control Group ◽

Protective Effects ◽

Chronic Restraint Stress ◽

Oxidative Stress Parameters ◽

Liver And Kidney ◽

Stress Damage ◽

The Brain

Restraint stress may be associated with elevated free radicals, and thus, chronic exposure to oxidative stress may cause tissue damage. Several studies have reported that carvacrol (CAR) has a protective effect against oxidative stress. The present study was designed to investigate the protective effects of CAR on restraint stress induced oxidative stress damage in the brain, liver, and kidney. For chronic restraint stress, rats were kept in the restrainers for 6 h every day, for 21 consecutive days. The animals received systemic administrations of CAR daily for 21 days. To evaluate the changes of the oxidative stress parameters following restraint stress, the levels of malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), and catalase (CAT) activities were measured in the brain, liver, and kidney. In the stressed animals that received vehicle, the MDA level was significantly higher (P<0.001) and the levels of GSH and antioxidant enzymes were significantly lower than the nonstressed animals (P<0.001). CAR ameliorated the changes in the stressed animals as compared with the control group (P<0.001). This study indicates that CAR can prevent restraint stress induced oxidative damage.

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Si Miao San Attenuates Inflammation and Oxidative Stress in Rats with CIA via the Modulation of the Nrf2/ARE/PTEN Pathway

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2021/2843623 ◽

2021 ◽

Vol 2021 ◽

pp. 1-11

Author(s):

Pan Shen ◽

Yao Huang ◽

Xin Ba ◽

Weiji Lin ◽

Kai Qin ◽

...

Keyword(s):

Oxidative Stress ◽

High Dose ◽

Collagen Induced Arthritis ◽

Rat Serum ◽

Oxidative Stress Parameters ◽

Tnf Α ◽

Cox 2 ◽

Synovial Tissues ◽

Stress Damage ◽

And Oxidative Stress

Objective. Si Miao San (SMS) is a traditional Chinese formula used in China to treat rheumatic diseases. To date, its mechanism in rheumatoid arthritis (RA) treatment is uncertain. Our study aims to assess the antiarthritic effects of SMS in experimental arthritic rats. Materials and Methods. SMS (8.63, 4.31, and 2.16 g/kg/day) was orally administered after the first immunization from day 14 to day 53. The effects of SMS on rats with collagen-induced arthritis (CIA) were evaluated by arthritis score and histological assessment. The levels of cytokines and anti-CII antibodies in rat serum were measured by ELISAs. The expression of oxidative stress parameters was detected by biochemical assay kits. The levels of Nrf2, HO-1, NQO1, and PTEN were determined by western blotting. Results. Medium- and high-dose SMS treatment significantly decreased arthritis scores and alleviated ankle joint histopathology in the rats with CIA. It inhibited the production of IL-6, TNF-α, COX-2, and PGE2 in rat serum. SMS also suppressed the expression of anti-CII antibodies IgG1 and IgG2a. Moreover, SMS significantly suppressed the levels of MDA and MPO in the synovial tissues while increasing the levels of SOD and CAT in the rats with CIA. The levels of Nrf2, HO-1, NQO1, and PTEN were upregulated by SMS in rat synovial tissues. Conclusions. This study demonstrated that SMS effectively alleviated the disease progression of CIA by decreasing the levels of proinflammatory cytokines and reducing oxidative stress damage, as indicated by IL-6, TNF-α, COX-2, and PGE2 levels; inhibiting the overproduction of MDA and MPO; and enhancing antioxidant enzymes by upregulating the Nrf2/ARE/PTEN signalling pathway.

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Hypercaloric Diet and Vitamin E Intake

Materiale Plastice ◽

10.37358/mp.19.4.5287 ◽

2019 ◽

Vol 56 (4) ◽

pp. 937-941

Author(s):

Catalina Radulescu ◽

Daniela Miricescu ◽

Alexandra Totan ◽

Andreea Cristina Didilescu ◽

Iulia-Ioana Stanescu ◽

...

Keyword(s):

Vitamin E ◽

Wistar Rats ◽

Glycolic Acid ◽

Redox Status ◽

Plga Nanoparticles ◽

Antioxidant Effect ◽

Daily Dose ◽

Group 2 ◽

Hypercaloric Diet ◽

Group 1

PLGA (poly-lactic-co-glycolic acid) nanoparticles represent an important synthetic biocomponent that has the potential to be a promising carrier of drugs, proteins, nucleic acids, due to its biodegradability and minimal side effects. The aim of our study was to observe the antioxidant effect of vitamin E loaded in PLGA nanoparticles administered over a period of 3 weeks in Wistar rats treated with a hypercaloric diet. Glutathione (GSH) and malondiadehyde (MDA) biomarkers determined from liver lysate were analyzed to evaluate the oxidative stress (OS) induced by the hypercaloric diet. The results of our study revealed a statistically significant increase for GSH and vitamin E in group 2 of Wistar rats receiving hypercaloric diet and a daily dose of vitamin E versus group 1 (p[0.005). The antioxidant effect of vitamin E was also observed by the statistically significant decrease of MDA in group 2 of Wistar rats compared with group 1. The daily dose of vitamin E has improved the liver redox status of group 2 Wistar rats.

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Does human myocardium show a gender dependent modification of oxidative stress parameters?

The Thoracic and Cardiovascular Surgeon ◽

10.1055/s-0034-1367393 ◽

2014 ◽

Vol 62 (S 01) ◽

Author(s):

K. Buschmann ◽

Y. Gramlich ◽

M. Oelze ◽

A. Daiber ◽

T. Münzel ◽

...

Keyword(s):

Oxidative Stress ◽

Human Myocardium ◽

Oxidative Stress Parameters ◽

Dependent Modification ◽

Stress Parameters

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Physical exercise protects dynamic balance and motor coordination of rats treated with vincristine

Revista Brasileira de Fisiologia do Exercício ◽

10.33233/rbfex.v19i5.4220 ◽

2020 ◽

Vol 19 (5) ◽

pp. 336

Author(s):

Luiza Minato Sagrillo ◽

Viviane Nogueira De Zorzi ◽

Luiz Fernando Freire Royes ◽

Michele Rechia Fighera ◽

Beatriz Da Silva Rosa Bonadiman ◽

...

Keyword(s):

Oxidative Stress ◽

Locomotor Activity ◽

Physical Exercise ◽

Motor Coordination ◽

Dynamic Balance ◽

Exercise Group ◽

Adult Rats ◽

The Central Nervous System ◽

Stress Damage ◽

Training Protocol

Physical exercise has been shown to be an important modulator of the antioxidant system and neuroprotective in several diseases and treatments that affect the central nervous system. In this sense, the present study aimed to evaluate the effect of physical exercise in dynamic balance, motor coordination, exploratory locomotor activity and in the oxidative and immunological balance of rats treated with vincristine (VCR). For that, 40 adult rats were divided into two groups: exercise group (6 weeks of swimming, 1h/day, 5 days/week, with overload of 5% of body weight) and sedentary group. After training, rats were treated with 0.5 mg/kg of vincristine sulfate for two weeks or with the same dose of 0.9% NaCl. The behavioral tests were conducted 1 and 7 days after each dose of VCR. On day 15 we carried out the biochemical analyzes of the cerebellum. The physical exercise was able to protect against the loss of dynamic balance and motor coordination and, had effect per se in the exploratory locomotor activity, and neutralize oxidative stress, damage DNA and immune damage caused by VCR up to 15 days after the end of the training protocol. In conclusion, we observed that previous physical training protects of the damage motor induced by vincristine.Key-words: exercise, oxidative stress, neuroprotection, cerebellum.

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