Essential Oil of Hypericum perforatum. The chemical composition and antimicrobial activity

2017 ◽  
Vol 68 (4) ◽  
pp. 687-692
Author(s):  
Lavinia Moleriu ◽  
Calin Jianu ◽  
Gabriel Bujanca ◽  
Gabriela Doros ◽  
Corina Misca ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Hypericum Perforatum ◽  
Extraction Yield ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Alpha Pinene ◽  
Resistant Species ◽  
Hypericum Perforatum L

The chemical composition and antimicrobial activity of the essential oil isolated by steam distillation from Hypericum perforatum L. (St John�s wort) growing wild in western Romania have been studied. The extraction yield was 0.41% (v/w) based on the dry plant material. The essential oil was analyzed by GC�MS, and a total of twenty-two components were identified. The major components were alpha-pinene (30.92%), beta-pinene (18.32%) and caryophyllene (15.26%). The antimicrobial activity of the H. perforatum essential oil was screened using the disk diffusion method against 7 common food-related bacteria and fungus. The analyzed EO possesses strong antimicrobial activity. Klebsiella pneumoniae and Pseudomonas aeruginosa were the most resistant species. The analyzed oil can represent an inexpensive natural source of antiseptic compounds, an alternative to synthetic preservatives.

scholarly journals Chemical composition and antimicrobial activity of essential oil from Pyrethrum pulchrum Ledeb.

2018 ◽  
Vol 19 (45) ◽  
pp. 38-43 ◽  
Author(s):  
Uugangerel Erdenetsogt ◽  
Choijamts Gotov ◽  
Kerstin Voigt ◽  
Stefan Bartram ◽  
Wilhelm Boland ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Diffusion Method ◽  
Oil Composition ◽  
Disk Diffusion Method ◽  
Gram Positive Bacteria ◽  
Aerial Parts ◽  
Total Oil ◽  
Sporidiobolus Salmonicolor

The chemical composition and antimicrobial activity of the essential oil from the aerial parts of Pyrethrum pulchrum Ledeb. were investigated. Dried plant material was hydro-distillated yielding 0.1% of essential oil. The oil was analyzed by GC-MS techniques. Fifty-five compounds were identified representing 99.7% of the total oil composition. Camphor was the predominant compound (33.9%) followed by linalool (21.1%) and α-pinene (9.0%). The antimicrobial activity of the oil was determined using the disk diffusion method against Gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus and Enterococcus faecalis), Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli), Mycobacterium vaccae and fungi (Candida albicans, Sporidiobolus salmonicolor and Penicillum notatum). The essential oil of P. pulchrum displays an intermediate activity against selected bacteria.

scholarly journals Quality testing of industrially produced essential oil of white pine (Pinus sylvestris L.) from the Republic of Srpska

2020 ◽  
Vol 12 (2) ◽  
Author(s):  
Vesna Gojković Cvjetković ◽  
Željka Marjanović-Balabań ◽  
Marijana Kapović-Solomuń ◽  
Ljiljaná́ Stanojevic ◽  
Jelená́ Stanojevic ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
White Pine ◽  
Disk Diffusion Method ◽  
Sesquiterpene Hydrocarbons ◽  
Zones Of Inhibition ◽  
The Republic

The quality of the essential oil obtained from white pine wood greenery from the territory of the Republic of Srpska was analyzed by examining the chemical composi- tion and antimicrobial activity of samples produced by the hydro-distillation process in industrial production conditions. The chemical composition of the essential oil was analyzed by GC/MS and GC/FID analysis. The antimicrobial activity of the ob- tained white pine oil was investigated for its possible use as an antimicrobial agent. The obtained results prove the presence of 37 components in the analyzed sam- ple, which represents 98.5% of the total components present. The most common are monoterpene hydrocarbons, followed by sesquiterpene hydrocarbons, much less represented are monoterpenes containing oxygen and aromatic compounds, while aliphatic esters are found only in traces. Antimicrobial activity was examined using the disk diffusion method. The results showed large zones of inhibition, both for the tested oil sample and for all eight analyzed bacterial strains (from 12.00 ± 0.00 to 17.33 ± 1.15mm), as well as for the fungal strain. The chemical composition and antimicrobial action of essential white pine oiled from plant material originating from the territory of the Republic of Srpska indicates its significant phytomedical potential.

scholarly journals Chemical composition and antimicrobial activity of the essential oil from leaves of Magnolia coriacea (Hung T. Chang & B. L. Chen) figlar growing in Vietnam

2020 ◽  
Vol 42 (3) ◽  
Author(s):  
Chu Thi Thu Ha ◽  
Bui Van Thanh ◽  
Dinh Thi Thu Thuy
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Inhibitory Concentration ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Test Microorganism ◽  
Leaf Essential Oil ◽  
Main Components ◽  
Susceptibility Assay

Leaf essential oil of Magnolia coriacea (Hung T. Chang & B. L. Chen) Figlar growing wild in the Bat Dai Son Nature Reserve, Ha Giang Province, Viet Nam was obtained by hydrodistillation and its chemical composition was analyzed using GC/MS. In total, 45 compounds were detected in the essential oil, accounting for 87.1% of the oil, in which 37 compounds were identified accounting for 66.9%. Bicyclogermacrene (12.6%) and spathulenol (17.0%) were the main components of the leaf essential oil of M. coriacea. Antimicrobial activity of the essential oil sample was tested against three microorganism strains using an agar disk diffusion method. The results show that the inhibitory zone diameters ranged from 8.5 to 20.5 mm. Median inhibitory concentration (IC50) and minimum inhibitory concentration (MIC) of the essential oil was determined using microdilution broth susceptibility assay against seven test microorganism strains. Bacillus subtilis had the highest sensitivity with IC50 and MIC values of 185.9 and 512 µg/mL, respectively.  

scholarly journals Chemical Composition and Antibacterial Activity of the Essential Oil of Espeletia nana

2012 ◽  
Vol 7 (5) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Alexis Peña ◽  
Luis Rojas ◽  
Rosa Aparicio ◽  
Libia Alarcón ◽  
José Gregorio Baptista ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Disk Diffusion Method ◽  
Gram Negative ◽  
Gram Positive Bacteria

The essential oil of the leaves of Espeletia nana Cuatrec, obtained by hydrodistillation, was analyzed by GC-MS, which allowed the identification of 24 components, which made up 99.9% of the oil. The most abundant compounds were α-pinene (38.1%), β-pinene (17.2%), myrcene (15.0%), spathulenol (4.2%), bicyclogermacrene (4.0%), α-zingiberene (4.0%), and γhimachalene (3.7%). Antibacterial activity was tested against Gram-positive and Gram-negative bacteria using the agar disk diffusion method. Activity was observed only against Gram-positive bacteria. MIC values were determined for Staphylococcus aureus ATCC 25923(200 μg/mL) and Enterococcus faecalis ATCC 29212 (600 μg/mL).

scholarly journals Antimicrobial Activity of two Mentha Species Essential Oil and its Dependence on Different Origin and Chemical Diversity

2018 ◽  
Vol 13 (8) ◽  
pp. 1934578X1801300 ◽  
Author(s):  
Mária Pl'uchtová ◽  
Teresa Gervasi ◽  
Qada Benameur ◽  
Vito Pellizzeri ◽  
Daniela Grul'ová ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Inhibition Zone ◽  
Chemical Diversity ◽  
Diffusion Method ◽  
Salmonella Spp ◽  
Disk Diffusion Method ◽  
Gram Positive Bacteria ◽  
Growth Inhibition Zone

Genus Mentha presents group of plants which are the most studied in family Lamiaceae. Aboveground parts are used for different purposes in pharmacy, food industry or confectionery. Most important is natural product extracted from leaves - essential oil (EO). The aim of presented experiment was to demonstrate different chemotype and compare antibacterial activity of two Mentha species EO. Plant samples were obtained from various environments – from Slovakia and from Italy. Dominant compounds were determined by GC/MS. The results showed high amount of menthol and menthone in tested Slovak peppermint EO. On the other hand, carvone and 1,8-cineole were determinate as dominant compounds in Italian spearmint EO. The antimicrobial activity of the EO was investigated by disc diffusion and broth micro dilution methods. EO was evaluated for their antibacterial activity against 7 microorganisms: Enterobacter cloacae, Salmonella spp., Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Streptococcus pyogenes. The determination results of antibacterial activity by agar disk diffusion method ranged from 7 to 14 mm of the growth inhibition zone. MIC of tested mint EO varied from 0.625 to 2.5 μg/mL. In addition, both EO showed relatively the same antibacterial activity against the selected Gram-negative bacteria. However, there is a variation in the antibacterial activity against Gram-positive bacteria.

scholarly journals Chemical Composition and Antibacterial Activity of Essential Oil of Anvillea radiata Coss. & Dur

2007 ◽  
Vol 2 (5) ◽  
pp. 1934578X0700200
Author(s):  
Fadwa El Hanbali ◽  
Ahmed El Hakmaoui ◽  
Fouad Mellouki ◽  
Lahoussine El Rhaffari ◽  
Mohamed Akssira
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Aerial Parts

The essential oil of the aerial parts of Anvillea radiata Coss. & Dur. was examined by GC-MS. Twenty-nine constituents were identified, representing 88.8% of the total. 6-Oxocyclonerolidol (66.6%) and 6-hydroxycyclonerolidol (11.4%) were found to be the major components. The disc diffusion method, used for antimicrobial activity testing, showed that the essential oil had a significant antibacterial activity against all tested microorganisms.

scholarly journals Comparison of Techniques and Solvents on the Antimicrobial and Antioxidant Potential of Extracts from Acacia dealbata and Olea europaea

Antibiotics ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 48 ◽  
Author(s):  
Anabela Borges ◽  
Helena José ◽  
Vera Homem ◽  
Manuel Simões
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Capacity ◽  
Plant Extracts ◽  
Olea Europaea ◽  
Antioxidant Activities ◽  
Extraction Yield ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Acacia Dealbata ◽  
Olea Europaéa

Ethnopharmacological use of plant natural extracts has been known since ancient times. The optimization of plant molecule extraction is fundamental in obtaining relevant extraction yields. The main purpose of this study was to understand the role of different extraction techniques (solid-liquid, ultrasound, Soxhlet, and microwave) and solvents (water, methanol, ethanol, acetone, dichloromethane, and hexane) on the antimicrobial and antioxidant activities of extracts from Olea europaea (olive) and Acacia dealbata (mimosa). Crude plant extracts were evaluated for their antimicrobial activity against Staphylococcus aureus and Escherichia coli by the disk diffusion method. The antioxidant capacity of the extracts was determined by ABTS (2,2-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid)) and DPPH (2,2-diphenyl-1-picrylhydrazyl) methods. In terms of extraction yield, ultrasound extraction and the solvents methanol, acetone (O. europaea) or water (A. dealbata) were found to be the best options. However, ethanol and acetone proved to be the best solvents to extract compounds with antimicrobial activity and antioxidant capacity, respectively (regardless of the extraction method employed). Soxhlet and microwave were the best techniques to extract compounds with antimicrobial activity, whereas any of the tested techniques showed the ability to extract compounds with antioxidant capacity. In most of the cases, both plant extracts (mimosa and olive) were more efficient against S. aureus than E. coli. In the present study, both mimosa and olive leaf crude extracts proved to have antimicrobial and antioxidant activities, increasing the demand of these natural products as a source of compounds with health benefits.

scholarly journals Biological activity of essential oil from Foeniculum vulgare

2021 ◽  
Vol 24 (2) ◽  
pp. 148-152
Author(s):  
Petra Borotová ◽  
Lucia Galovičová ◽  
Veronika Valková ◽  
Hana Ďúranová ◽  
Nenad Vuković ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Essential Oil ◽  
Vapour Phase ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Volatile Components ◽  
Foeniculum Vulgare ◽  
Disk Diffusion Method ◽  
Bacterial Growth Inhibition

Abstract Foeniculum vulgare Mill. is a medicinal plant, used as a flavouring agent. The essential oil from F. vulgare has potential antimicrobial and insecticidal effects, and can be used in food industry in order to protect the food resources and food products against microbial and pest’s contamination. The aim of the research was to characterize the volatile components of F. vulgare essential oil by Gas Chromatography/Mass Spectrometry (GC/MS) and Gas Chromatography (GC-FID) and to observe the antimicrobial activity by disk diffusion method and in vapour phase. Also, insecticidal activity of the vapour phase of the essential oil of F. vulgare was detected. We found that major components of the essential oil from F. vulgare were trans-anethole (73.6%), fenchone (6.0%), and limonene (5.7%). Antimicrobial activity on gram-positive, gram-negative, and yeasts was weak in liquid phase, but vapour phase showed stronger activity against B. subtilis at the concentration 250 μL.L−1 (98.65% of bacterial growth inhibition). Vapour phase of essential oil was effective against insects, where 25% concentration had 80% lethality.

scholarly journals In vitro Synergistic Antimicrobial Activity of Romanian Propolis and Antibiotics against Escherichia coli Isolated from Bovine Mastitis

2015 ◽  
Vol 43 (2) ◽  
pp. 327-334 ◽  
Author(s):  
Mihaela NICULAE ◽  
Laura STAN ◽  
Emoke PALL ◽  
Anamaria Ioana PAȘTIU ◽  
Iulia Maria BALACI ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Bovine Mastitis ◽  
Antibacterial Properties ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Ethanolic Extracts

The study was aimed to characterize the chemical composition and the antimicrobial activity of Romanian propolis ethanolic extracts (EEP) against antibiotic-sensitive and antibiotic-resistant E. coli strains isolated from bovine mastitis. The preliminary antimicrobial screening was performed by a disk diffusion method, followed by determination of minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) based on broth microdilution assay; further, the synergistic action of propolis with antimicrobial drugs was assessed by a disk diffusion method on agar containing subinhibitory concentrations of propolis. For the chemical characterisation of EEP, the flavonoids (flavones/flavonols, flavanones/dihydroflavonols) and total phenolics were evaluated by spectrophotometric methods. The phenolic compounds of these extracts were also determined using HPLC. The results indicated for Romanian propolis ethanolic extracts the typical poplar composition profile with flavonoids and phenolic acids as main biological active compounds, with chromatographic analysis data confirmed also spectrophotometrically. In addition, positively correlated with the chemical composition, a strong antimicrobial efficacy was exhibited towards E. coli strains, along with interesting synergistic interaction with antibiotics that can be further investigated to obtain propolis-based formulation with antibacterial properties. Subsequent in vitro and in vivo studies evaluating the safety and efficacy are intended to consider propolis in veterinary therapeutic protocols.

scholarly journals Chemical Composition and Antimicrobial Activity of Essential Oil of Heracleum rigens

2012 ◽  
Vol 7 (7) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Nataraj Jagannath ◽  
Hanumanthaiah Ramakrishnaiah ◽  
Venkatarangaiah Krishna ◽  
Prameela Javarayi Gowda
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Diffusion Method ◽  
Bornyl Acetate ◽  
Klebsiella Pneumonia ◽  
Oil Composition ◽  
Bacterial Strains ◽  
Octyl Acetate ◽  
Total Oil

The essential oil was extracted from the seeds of Heracleum rigens by hydrodistillation and a total of twenty compounds accounting for 98.5% of the total oil composition were identified. Physicochemical properties and chemical composition of the oil was determined by a combination GC/FID and GC/MS analysis. The major compounds identified were bornyl acetate (51.2%), α-pinene (22.6%), limonene (9.62%), octyl acetate (3.94%), p-cymene (2.85%) and γ-terpinene (1.93%). The antimicrobial activity of the oil was screened by the disc diffusion method against nine pathogenic bacterial strains. Maximum antimicrobial activity was noted against Klebsiella pneumonia and Bacillus subtillis. This investigation corroborates the traditional claim of H. rigens as an effective antimicrobial agent.

Sign in / Sign up

Export Citation Format

Share Document