The Effect of Exercising on Oxidative Stress Status and Pain in a Valproic-acid Induced Model of Autism Possible relevance of oxytocin

2017 ◽  
Vol 68 (9) ◽  
pp. 2028-2033 ◽  
Author(s):  
Iulia Antioch ◽  
Dana Ababei ◽  
Radu Lefter ◽  
Alin Ciobica ◽  
Cezar Honceriu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Valproic Acid ◽  
Pain Perception ◽  
Visceral Pain ◽  
Specific Activity ◽  
Female Rats ◽  
Hot Plate Test ◽  
Thermal Pain ◽  
Oxidative Stress Status ◽  
Local Inflammatory Reaction

In this report we will describe the effect of exercising (6 days, each day 3 separate training phases of 5 minutes each, on an adapted treadmill at 1.0 m/s) on oxidative stress status from the temporal lobe (expressed through 3 main parameters: superoxide dismutase (SOD), glutathione peroxidase (GPX) and malondialdehyde (MDA), as a marker of lipid peroxidation) and pain (as determined through 2 specific behavioural tasks such as hot plate test for the supraspinal acute thermal pain and the intra abdominal Zymosan administration for eliciting a local inflammatory reaction following responses to inflammatory visceral pain) in a rat valproic-acid induced perinatal model of autism, also trying to emphasize a possible implication of oxytocin in this complex pathological picture. We demonstrated here an increased oxidative stress status, as a result of treadmill exercising, in a VPA rat induced model of autism, as demonstrated mainly by a significant decrease in the specific activity of SOD (for the exercised VPA female rats), as well as a significant decrease of GPX specific activity in the male VPA exercised rats, when compared to non-exercised VPA groups, as well as the fact that the aforementioned series of exercises did not resulted in any changes of the pain perception of this rat models of autism, as studied in 2 pain-related behavioural tasks, independent to the gender of the rats with VPA model of autism.

scholarly journals Autistic-Like Behaviors, Oxidative Stress Status, and Histopathological Changes in Cerebellum of Valproic Acid Rat Model of Autism Are Improved by the Combined Extract of Purple Rice and Silkworm Pupae

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Nartnutda Morakotsriwan ◽  
Jintanaporn Wattanathorn ◽  
Woranan Kirisattayakul ◽  
Kowit Chaisiwamongkol
Keyword(s):  
Oxidative Stress ◽  
Valproic Acid ◽  
Purkinje Cell ◽  
Rat Model ◽  
Cell Loss ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Silkworm Pupae ◽  
Oxidative Stress Status ◽  
Purkinje Cell Loss

Due to the crucial role of oxidative stress on the pathophysiology of autism and the concept of synergistic effect, the benefit of the combined extract of purple rice and silkworm pupae (AP1) for autism disorder was the focus. Therefore, we aimed to determine the effect of AP1 on autistic-like behaviors, oxidative stress status, and histopathological change of cerebellum in valproic acid (VPA) rat model of autism. VPA was injected on postnatal day (PND) 14 and the animals were orally given AP1 at doses of 50, 100, and 200 mg·kg−1BW between PND 14 and PND 40. The autism-like behaviors were analyzed via hot-plate, rotarod, elevated plus-maze, learning, memory, and social behavior tests. Oxidative stress and the histological change in the cerebellum were assessed at the end of study. AP1 treated rats improved behaviors in all tests except that in hot-plate test. The improvement of oxidative stress and Purkinje cell loss was also observed in the cerebellum of VPA-treated rats. Our data suggest that AP1 partially reduced autism-like behaviors by improving oxidative stress and Purkinje cell loss. Further research is required to identify the active ingredients in AP1 and gender difference effect.

Oxidative Stress Status in Patients with Choledocholithiasis: Before and After Endoscopic Sphincterotomy and Biliary Clearance

2018 ◽  
Vol 69 (8) ◽  
pp. 2172-2176
Author(s):  
Catalin Victor Sfarti ◽  
Alin Ciobica ◽  
Carol Stanciu ◽  
Gheorghe G. Balan ◽  
Irina Garleanu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Endoscopic Sphincterotomy ◽  
Specific Activity ◽  
Oxidative Stress Marker ◽  
Control Group ◽  
Oxidative Stress Parameter ◽  
Extrahepatic Cholestasis ◽  
Biliary Clearance ◽  
Oxidative Stress Status ◽  
Before And After

Choledocholithiasis may cause biliary obstruction which leads to hepatocellular injury. Oxidative stress has been proposed as a possible mechanism involved in this disorder. This study evaluates the oxidative stress burden in patients with choledocholithiasis and secondary cholestasis, before and after endoscopic sphincterotomy. Experimental part: Patients diagnosed with choledocholithiasis and secondary extrahepatic cholestasis were included in the study between January 1st 2016 and October 31st 2016. In all patients oxidative stress markers were collected within 2 hours before and 48 hours after therapeutic ERCP. Selected markers were superoxide dismutase (SOD), glutathione peroxidase (GPX) and malondialdehyde (MDA). The results were compared to those from a group of 40 healthy subjects. Significantly lower concentrations of SOD (p = 0.03) and GPX (p [ 0.0001) activities, associated with an increased level of MDA level (p [ 0.0001) were shown in patients before biliary clearance compared with the healthy control group. After ERCP the only oxidative stress parameter which showed improvement was the SOD specific activity (p = 0.037). This study shows that extrahepatic cholestasis secondary to choledocholithiasis is associated with increased oxidative stress status. After biliary clearance one oxidative stress marker was significantly improved (SOD), suggesting a possible antioxidant effect of such procedure.

Increase in non-transferrin bound iron and the oxidative stress status in epilepsy patients treated using valproic acid monotherapy

2011 ◽  
Vol 49 (04) ◽  
pp. 268-276 ◽  
Author(s):  
S. Ounjaijean ◽  
T. Westermarck ◽  
M. Partinen ◽  
E. Plonka-Poltorak ◽  
P. Kaipainen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Valproic Acid ◽  
Oxidative Stress Status

scholarly journals The Relevance of Oxidative Stress Status in One Week and One Month Alcohol Abstinent Patients

2014 ◽  
Vol 33 (3) ◽  
pp. 284-290 ◽  
Author(s):  
Ovidiu Alexinschi ◽  
Roxana Chirita ◽  
Alin Ciobica ◽  
Padurariu Manuela ◽  
Romeo Dobrin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Alcohol Withdrawal ◽  
Antioxidant Defense ◽  
Defense Mechanism ◽  
Specific Activity ◽  
Oxidative Stress Marker ◽  
Control Group ◽  
Stress Markers ◽  
Oxidative Stress Status ◽  
Markers Of Oxidative Stress

Abstract Background: Although it is generally accepted that there is an increased oxidative stress status in alcoholics, the separate relevance of oxidative stress following alcohol withdrawal is still not understood to this date. There are reports stating that the increased oxidative stress status in alcoholics may persist independently of the constant presence of alcohol intake, while on the other side, it was demonstrated that the antioxidant defense mechanism could significantly increase after alcohol withdrawal. Methods: In the present work, we were interested in studying the relevance of oxidative stress status in the alcohol withdrawal processes, by determining some oxidative stress markers (two antioxidant enzymes: superoxide dismutase - SOD and glutathione peroxidase - GPX and a lipid peroxidation maker - MDA) after one week and one month of abstinence, as compared to the baseline and a control group of subjects. Results: Our data confirmed the increased oxidative stress status in alcoholic patients and, more importantly, we de m - onstrated here a significant decrease of the oxidative stress status one week and one month following the withdrawal, as showed by a significant increase in the specific activity of SOD (p<0.003), as well as by a decrease in MDA levels (p<0.019). Still, in the case of all three markers of oxidative stress status which we determined, the levels after one week or one month of abstinence were significantly altered when compared to controls. Conclusions: This suggests that severe and prolonged deficiency in the oxidative stress marker levels needs longer than one month of abstinence to normalize.

scholarly journals Leptin and camel milk abate oxidative stress status, genotoxicity induced in valproic acid rat model of autism

2018 ◽  
Vol 32 ◽  
pp. 205873841878551 ◽  
Author(s):  
Mohamed A Hamzawy ◽  
Yasmin B El-Ghandour ◽  
Sekena H Abdel-Aziem ◽  
Zoba H Ali
Keyword(s):  
Oxidative Stress ◽  
Valproic Acid ◽  
Rat Model ◽  
Positive Control ◽  
Female Rats ◽  
Camel Milk ◽  
Male Rats ◽  
Control Group ◽  
Autistic Behaviour ◽  
Group B

The aspect of treatment of autistic behaviour was investigated using valproic acid rat model of pregnant female rats. Two main groups (10 male rats/group) were treated for 6 days and then divided into six subgroups. The first group of normal rats was divided into three subgroups: (A) – control group, (B) – treated with camel milk (CAM; 2 mL/p.o) and (C) – treated with leptin (1000 µg/kg i.p) twice daily. The second group of autistic rats was randomly distributed into four subgroups as follows: (D) – positive control (autistics rats), (E) – treated with CAM, (F) – treated with a moderate dose of leptin and (G) – treated with a higher dose of leptin. Autistic behaviours of male offspring were checked by grooming and elevated pulz maze tests. Valproic acid (VPA)-induced autistic rats showed severe changes in oxidative stress markers, neurotransmitters and inflammatory cytokines, besides genotoxic manifestation of expression of tumour necrosis factor (TNF)-α, Bax and caspase-3. Leptin or CAM alone showed no signs of toxicity. CAM showed pronounced improvement in control rats than control itself. Leptin or CAM treatment of autistic animals showed a significant improvement of all measured parameters and genetic expression values. The improvement was pronounced in animals treated with CAM. These results suggest that CAM is a potential therapeutic candidate for autism via regulation of inflammatory and apoptotic pathways. Leptin plays an essential role in alleviation of autistic behaviour through antioxidant effects.

scholarly journals Oxytocin Differentiated Effects According to the Administration Route in a Prenatal Valproic Acid-Induced Rat Model of Autism

Medicina ◽  
2020 ◽  
Vol 56 (6) ◽  
pp. 267
Author(s):  
Radu Lefter ◽  
Alin Ciobica ◽  
Iulia Antioch ◽  
Daniela Carmen Ababei ◽  
Luminita Hritcu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Valproic Acid ◽  
Rat Model ◽  
Short Term Memory ◽  
Therapeutic Potential ◽  
Gastrointestinal Transit ◽  
Autism Spectrum ◽  
Temporal Area ◽  
Oxidative Stress Status ◽  
And Oxidative Stress

Background and objectives: The hormone oxytocin (OXT) has already been reported in both human and animal studies for its promising therapeutic potential in autism spectrum disorder (ASD), but the comparative effectiveness of various administration routes, whether central or peripheral has been insufficiently studied. In the present study, we examined the effects of intranasal (IN) vs. intraperitoneal (IP) oxytocin in a valproic-acid (VPA) autistic rat model, focusing on cognitive and mood behavioral disturbances, gastrointestinal transit and central oxidative stress status. Materials and Methods: VPA prenatally-exposed rats (500 mg/kg; age 90 days) in small groups of 5 (n = 20 total) were given OXT by IP injection (10 mg/kg) for 8 days consecutively or by an adapted IN pipetting protocol (12 IU/kg, 20 μL/day) for 4 consecutive days. Behavioral tests were performed during the last three days of OXT treatment, and OXT was administrated 20 minutes before each behavioral testing for each rat. Biochemical determination of oxidative stress markers in the temporal area included superoxide dismutase (SOD), glutathione peroxidase (GPx) and malondialdehyde (MDA). A brief quantitative assessment of fecal discharge over a period of 24 hours was performed at the end of the OXT treatment to determine differences in intestinal transit. Results: OXT improved behavioral and oxidative stress status in both routes of administration, but IN treatment had significantly better outcome in improving short-term memory, alleviating depressive manifestations and mitigating lipid peroxidation in the temporal lobes. Significant correlations were also found between behavioral parameters and oxidative stress status in rats after OXT administration. The quantitative evaluation of the gastrointestinal (GI) transit indicated lower fecal pellet counts in the VPA group and homogenous average values for the control and both OXT treated groups. Conclusions: The data from the present study suggest OXT IN administration to be more efficient than IP injections in alleviating autistic cognitive and mood dysfunctions in a VPA-induced rat model. OXT effects on the cognitive and mood behavior of autistic rats may be associated with its effects on oxidative stress. Additionally, present results provide preliminary evidence that OXT may have a balancing effect on gastrointestinal motility.

Amygdala activation by corticosterone alters visceral and somatic pain in cycling female rats

2011 ◽  
Vol 300 (6) ◽  
pp. G1080-G1085 ◽  
Author(s):  
Jenny K. Gustafsson ◽  
Beverley Greenwood-Van Meerveld
Keyword(s):  
Menstrual Cycle ◽  
Estrous Cycle ◽  
Pain Perception ◽  
Visceral Pain ◽  
Female Rats ◽  
Dorsal Margin ◽  
Somatic Pain ◽  
Amygdala Activation ◽  
Enhanced Sensitivity ◽  
Visceromotor Response

Irritable bowel syndrome (IBS) is often seen in women, and symptom severity is known to vary over the menstrual cycle. In addition, activation of the hypothalamic-pituitary-adrenal (HPA) axis enhances symptomology and patients with IBS have increased activation of the amygdala, a brain region known to facilitate HPA output. However, little is known about the effects of amygdala activation during different stages of the menstrual cycle. We therefore investigated the effects of amygdala activation on somatic and visceral pain perception over the rat estrous cycle. Female Wistar rats were implanted with either corticosterone (Cort) or cholesterol as a control onto the dorsal margin of the central amygdala. Visceral sensitivity was quantified by recording the visceromotor response (VMR) to colorectal distension (CRD) and somatic sensitivity was assessed via the Von Frey test. In cholesterol controls, both visceral and somatic sensitivity varied over the estrous cycle. Rats in proestrus/estrus responded to CRD with an increased VMR compared with rats in metestrus/diestrus. Somatic sensitivity followed a similar pattern with enhanced sensitivity during proestrus/estrus compared with metestrus/diestrus. Elevated amygdala Cort induced visceral hypersensitivity during metestrus/diestrus but had no effect during proestrus/estrus. In contrast, elevated amygdala Cort increased somatic sensitivity during both metestrus/diestrus and proestrus/estrous. These results suggests that amygdala activation by Cort eliminates spontaneously occurring differences in visceral and somatic pain perception, which could explain the lowered pain thresholds and higher incidence of somatic pain observed in women with IBS.

scholarly journals SENSITIVITY OF BREAST CANCER STEM CELLS (CD24-/CD44+) TO DOXORUBICIN IS ASSOCIATED WITH OXIDATIVE STRESS STATUS

2019 ◽  
pp. 91-96
Author(s):  
RESDA A. SYAHRANI ◽  
MELVA LOUISA ◽  
SEPTELIA I. WANANDI
Keyword(s):  
Oxidative Stress ◽  
Mrna Expression ◽  
Manganese Superoxide Dismutase ◽  
Specific Activity ◽  
Trypan Blue Exclusion ◽  
Doxorubicin Treatment ◽  
Manganese Superoxide ◽  
Oxidative Stress Status ◽  
Dichlorofluorescein Diacetate ◽  
Ellman’S Method

Objective: The aim of this study was to analyze the sensitivity of BCSCs to doxorubicin and its association with oxidative stress. Methods: BCSCs (CD24-/CD44+) were treated with doxorubicin every 2 d for 14 d. The determination of cell viability was performed using a trypan blue exclusion assay. The levels of reactive oxygen species (ROS) were measured using a dihydroethidium (DHE) and a 2’,7’-dichlorofluorescein diacetate (DCFH-DA) probes. Manganese superoxide dismutase (MnSOD) mRNA expression and specific activity were also analyzed. Glutathione (GSH) level was measured using Ellman’s method. Results: The viability of the BCSCs decreased after 2 d of treatment with doxorubicin, but started to increase after 8 d. After 8 d of doxorubicin treatment, the ROS level in the BCSCs decreased, while the MnSOD specific activity increased. In addition, the MnSOD mRNA expression and GSH level were suppressed after 8 d of treatment. Conclusion: Doxorubicin treatment induced cytotoxicity after 2 d by increasing the superoxide levels of the BCSCs. After 8 d of treatment, the sensitivity of BCSCs to doxorubicin decreased due to the suppressed oxidative stress from the enhanced antioxidant activity of the MnSOD.

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