scholarly journals Leptin and camel milk abate oxidative stress status, genotoxicity induced in valproic acid rat model of autism

2018 ◽  
Vol 32 ◽  
pp. 205873841878551 ◽  
Author(s):  
Mohamed A Hamzawy ◽  
Yasmin B El-Ghandour ◽  
Sekena H Abdel-Aziem ◽  
Zoba H Ali
Keyword(s):  
Oxidative Stress ◽  
Valproic Acid ◽  
Rat Model ◽  
Positive Control ◽  
Female Rats ◽  
Camel Milk ◽  
Male Rats ◽  
Control Group ◽  
Autistic Behaviour ◽  
Group B

The aspect of treatment of autistic behaviour was investigated using valproic acid rat model of pregnant female rats. Two main groups (10 male rats/group) were treated for 6 days and then divided into six subgroups. The first group of normal rats was divided into three subgroups: (A) – control group, (B) – treated with camel milk (CAM; 2 mL/p.o) and (C) – treated with leptin (1000 µg/kg i.p) twice daily. The second group of autistic rats was randomly distributed into four subgroups as follows: (D) – positive control (autistics rats), (E) – treated with CAM, (F) – treated with a moderate dose of leptin and (G) – treated with a higher dose of leptin. Autistic behaviours of male offspring were checked by grooming and elevated pulz maze tests. Valproic acid (VPA)-induced autistic rats showed severe changes in oxidative stress markers, neurotransmitters and inflammatory cytokines, besides genotoxic manifestation of expression of tumour necrosis factor (TNF)-α, Bax and caspase-3. Leptin or CAM alone showed no signs of toxicity. CAM showed pronounced improvement in control rats than control itself. Leptin or CAM treatment of autistic animals showed a significant improvement of all measured parameters and genetic expression values. The improvement was pronounced in animals treated with CAM. These results suggest that CAM is a potential therapeutic candidate for autism via regulation of inflammatory and apoptotic pathways. Leptin plays an essential role in alleviation of autistic behaviour through antioxidant effects.

scholarly journals In vivo evidences of Curcuma longa on oxidative stress in STZ-induced diabetes on sperm parameters in male wistar rats

2017 ◽  
Vol 6 (3) ◽  
pp. 1045-1051
Author(s):  
A.J. Olanrewaju ◽  
S.Y. Olatunji ◽  
J.O. Owolabi ◽  
A.T. Oluwatosin ◽  
W.C. Amaechi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Sperm Quality ◽  
Curcuma Longa ◽  
Diabetic Control ◽  
Positive Control ◽  
Sperm Parameters ◽  
Male Rats ◽  
Control Group ◽  
Alternative Source

Several conditions such as chemotherapy and toxins can interfere with spermatogenesis and reduce sperm quality and production. In the case where natural antioxidant response cannot manage oxidative stress and free radical overload, oxidative damage occurs and this begins the genesis of many diseases of which diabetes is one of them. This has awakened the interest of researchers to the use of an alternative source of medicine and herbal medicine. Medicinal use of Curcuma longa dates back to ancient China and India; its constituents are stated to have anti-hepatotoxic, anti-inflammatory, stimulant, and antioxidant and used since ancient time as medicinal and nutritive origins knowing to possess androgenic activities and have well effect in diseases treatment in more countries world-wide. As an antioxidant Curcuma longa possible has a useful effect on spermatogenesis and sperm parameters. Wistar male rats (n=24) were allocated into six groups, positive control (n=4), diabetic control (n=4) and experimental groups (n=20), that subdivided into groups of 4 that received treatment of Curcuma longa rhizome powder with or without STZ-induced diabetes in the dosages (25 and 100mg/kg/day) for 21 consecutive days. In twenty-second day, the testes were removed and semen was collected from epididymis and prepared for analysis. The percentage of sperm viability and motility in the treatment groups increased mildly with a significance of: (p<0.05) in comparison to control group and with the diabetic group being critically lower than those in control group. This suggested that Curcuma longa may be promising in enhancing sperm health parameters.Keywords: Turmeric, oxidative stress, semen, STZ-induced diabetes, antioxidant

A Recombinant Humanized Monoclonal Antibody for Treatment of Endometriosis in a Rat Model

2011 ◽  
Vol 3 (3) ◽  
pp. 143-150
Author(s):  
Hatice Işık ◽  
Ozlem Moraloglu ◽  
Sevtap Kilic ◽  
Ali Seven ◽  
Muzaffer Caydere ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Rat Model ◽  
Female Rats ◽  
Control Group ◽  
Ki 67 ◽  
Beneficial Effects ◽  
Anti Vegf ◽  
Humanized Monoclonal Antibody ◽  
Group A ◽  
Group B

Aim Angiogenesis plays an important role in the pathogenesis of endometriosis. Thus, the inhibition of angiogenesis may prevent endometriosis. Bevacizumab is a recombinant humanized monoclonal antibody against VEGF. The aim of this study is to investigate the efficacy of anti-VEGF therapy on the prophylaxis and treatment of endometriotic foci in a rat model. Methods This experimental study is prospective, randomized, and placebo-controlled. Thirty-six Wistar-Albino female rats were divided into 3 groups. Experimental endometriosis was induced by the implantation of autologous endometrial tissue. The bevacizumab administration route was intraperitoneal. Group A was the prophylaxis group; Group B was the treatment group, and Group C was the control group. The volumes of the implants as well as their VEGF and Ki-67 immunohistochemical staining are main outcome measures. Results The volumes of the lesions were smaller in Group A than Group C [P<.05]. The volumes of endometriotic foci in Group B were smaller than in Group C [P<.05]. Bevacizumab caused regression and atrophy of the endometriotic lesions. After the treatment the histopathologic and immunohistochemical scores in Group B were less than before treatment and less than the scores in Group C. Conclusions Bevacizumab treatment had a regressive effect on the endometriotic implants. As an anti-VEGF agent, bevacizumab has beneficial effects on the prophylaxis and treatment of endometriosis.

scholarly journals Oxidative stress profile following the co-administration of cisplatin and resveratrol in female rats: a preliminary study

2021 ◽  
Vol 25 (2) ◽  
pp. 134-145
Author(s):  
Izuchukwu Azuka Okafor ◽  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Normal Control ◽  
Intraperitoneal Injection ◽  
Female Rats ◽  
Control Group ◽  
Stress Profile ◽  
Body Tissues ◽  
Group A ◽  
Group B

Introduction: Cisplatin is one of the most widely used drugs for the treatment of various cancers but has oxidative tissue damage as one of its side effects. This study investigated the oxidative stress profile in some important body tissues following the co-administration of cisplatin (CIS) and resveratrol (RSV). Methods: Thirty-five adult female rats with an average body weight of 162g were divided into 5 groups (n=7) and used for this experimental study. Group A served as the normal control group and received distilled water only. Group B received only a single dose intraperitoneal injection of 10mg/kg CIS. Groups C, D and E were orally given 5, 10 and 20mg/kg of RSV respectively for 7 days, starting 24h after a single CIS dose intraperitoneal injection of 10mg/kg. Selected body tissues were harvested for oxidative stress profiling at the end of the experiment. Results: CIS significantly increased malondialdehyde levels and decreased glutathione, superoxide dismutase and catalase levels in all the tissues assessed (ovary, uterus, liver, kidney, pancreas, stomach and spleen) when compared to the normal control. The RSV treatment caused the reversal of these effects; malondialdehyde levels were significantly decreased, while glutathione, superoxide dismutase and catalase levels were significantly increased across all the examined tissues. Conclusion: RSV at different doses could be effective in the management of CIS-induced oxidative stress and lipid peroxidation across some body tissues. However, this effect may be dependent on the dose of CIS and RSV.

scholarly journals Administration of red rosella’s petals extract (Hibiscus sabdariffa) increase level of superoxide dismutase (SOD) wistar rats which induced by repeteadly heated cooking oil

2017 ◽  
Vol 4 (2) ◽  
pp. 13 ◽  
Author(s):  
Novera Herdiani ◽  
Bambang Wirjatmadi ◽  
Merryana Adriani
Keyword(s):  
Oxidative Stress ◽  
Treatment Group ◽  
Wistar Rats ◽  
Saturated Fatty Acids ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Control Group ◽  
Standard Diet ◽  
Cooking Oil

Background:  repeatedly heated cooking oil is cooking oil that is used many times, had saturated fatty acids containing free radicals. Red rosella petals extract contains antioxidants to minimize the effects of repeatedly heated cooking oil that can raise the levels of SOD. The aim of this study was to analyzed the effect of red rosella’s petals extract against oxidative stress of Wistar rats were induced by repeatedly heated cooking oil with examine levels of  serum SOD.This research was conducted True Experimental Laboratory with a completely randomized design (CRD) and use the post test. The sample consisted of 24 male rats were divided 4 groups: negative control (fed with standard diet); positive control (fed with standard diet + administered by repeatedly heated cooking oil as much as 2.1 ml / kg bw); treatment group dose I (fed with standard diet + administered by red rosella extract dose 540 mg / kg bw + repeatedly heated cooking oil as much as 2.1 ml / kg bw), and treatment group dose II (fed with standard diet + administered by red rosella extract dose 810 mg / kg bw + repeatedly heated cooking oil as much as 2.1 ml / kg bw). Analysis of data using statistical test One Way ANOVA and Tukey HSD (α = 0.05).Results: There were significant differences (p = 0.000) between groups. Red rosella extract treatment group dose 810 mg / kg bw and 540 mg / kg bw were significantly different from the positive control (p = 0.000). Red rosella extract group dose 540 mg / kg bw was significantly different from the negative control (p = 0.000). Red rosella extract group dosage of 810 mg / kg bw was not differ significantly from the control group (p = 0.171).Conclusion: The red rosella’s petals extract dose of 810 mg / kg bw and dose of 540 mg / kg bw were able to prevent oxidative stress. Optimal deose of red rosella petals extract dose of 810 mg / kg bw was the most effective, because the higher antioxidant activity (67.33%) at dose 810 mg / kg bw dose than 540 mg / kg bw.

scholarly journals Gambir Extract (Uncaria Gambir) Decreases Inflammatory Response and Increases Gastric Mucosal Integrity in Wistar Rats - Model Gastritis

2019 ◽  
Vol 7 (19) ◽  
pp. 3149-3152 ◽  
Author(s):  
Liniyati Oswari ◽  
Rachmat Hidayat ◽  
Fatmawati Fatmawati ◽  
Lusia Hayati ◽  
Bella Safira Alisa
Keyword(s):  
Prostaglandin E2 ◽  
Rat Model ◽  
Wistar Rat ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Tnf Alpha ◽  
Control Group ◽  
Control Group Design ◽  
Alpha Level

BACKGROUND: Uncaria gambir (local name: gambir) is a plant native to Sumatera, Malaya and Borneo. This plant is potential as local wisdom for therapeutics. In Sumatera, gambir was used as a traditional treatment for fever, diarrhoea, diabetics and wound healing. AIM: To explore the efficacy of gambir extract on TNF alpha level, prostaglandin E2 level, lesson area, body weight, lipid profile and leptin level in Wistar rat-model gastritis. METHODS: This study was an experimental study, with a pre-post-test control group design. The subjects in this study were 30 male rats, 8 weeks old, weight 150-200 gram. Rats were administered with gambir extract at the dose of 20, 40 and 80 mg/kg BW/day for 3 days. Gambir was extracted by maceration methods. Statistical analysis was performed by SPSS 18. RESULTS: Gambir extract at the dose of 80 mg/kg BW exhibited the highest efficacy in reducing TNF alpha level, lesion area and increasing prostaglandin E2 level compared to gambir extract at doses of 20 mg/kg BW, 400 mg/kg BW, negative control, and positive control. CONCLUSION: Gambir extract was effective in reducing TNF alpha level, lesson area, and increasing prostaglandin E2 level in Wistar rat-model gastritis.

scholarly journals Test of the Aphrodisiac Effect of Ethanol Extract of Roots, Stems and Leaves of Packaged Plants (Smilax rotundifolia) on Male White Rats (Sprague Dawley)

2021 ◽  
Vol 5 (4) ◽  
pp. 280-285
Author(s):  
Kiki Rizki Handayani ◽  
Ayu Wulandari
Keyword(s):  
Ethanol Extract ◽  
Positive Control ◽  
Female Rats ◽  
Colloid Solution ◽  
Control Group ◽  
Sprague Dawley ◽  
Plant Parts ◽  
White Rats ◽  
Male And Female Rats ◽  
Group B

Developing countries use medicinal plants for aphrodisiac treatment, one of which is a pack plant (Smilax rotundifolia) which is commonly used to enlarge male genitalia in Papua Indonesia. Because these claims are not scientifically tested and proven, at this time This study aims to determine the effect of the ethanol extract of S. rotundifolia stem and leaf tubers on the parameters of sexual behavior in male Sprague Dawley rats. As well as fear the most effective plant parts have aphrodisiac activity. Thirty rats were divided into 5 groups (A-E) consisting of group A given 0.5% Na CMC colloid solution (normal control), group B given X-gra® suspension dosage 51.37 mg / kgBW (control positive), group C was given root ethanol extract dosage 200mg / kgBB, group D was given stem ethanol extract dosage 200mg / kgBB and group E was given leaf ethanol extract dosage 200mg / kgBB. The aphrodisiac test was observed on the 31st day with a ratio of male and female rats (1: 2). All parameters tested in the extract group showed significant differences with the normal group. This showed an aphrodisiac effect in all extract groups. The 200 mg / KgBB stem extract group reduced latent rates and intromission mounts as well as increased copulation rates, showing a marked increase in the number of intromissions, reduced ejaculatory latency and inter-intromission interval, and increased ejaculation frequency. which was not significant in the X-gra group (positive control) (p

Edaravone ameliorates the adverse effects of valproic acid toxicity in small intestine

2014 ◽  
Vol 34 (6) ◽  
pp. 654-661 ◽  
Author(s):  
S Oktay ◽  
B Alev ◽  
S Tunali ◽  
E Emekli-Alturfan ◽  
T Tunali-Akbay ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Valproic Acid ◽  
Small Intestine ◽  
Free Radical ◽  
Biochemical Parameters ◽  
Radical Reaction ◽  
Female Rats ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Free Radical Reaction

Valproic acid (VPA) is a drug used for the treatment of epilepsy, bipolar psychiatric disorders, and migraine. Previous studies have reported an increased generation of reactive oxygen species and oxidative stress in the toxic mechanism of VPA. Edaravone, a free radical scavenger for clinical use, can quench free radical reaction by trapping a variety of free radical species. In this study, effect of edaravone on some small intestine biochemical parameters in VPA-induced toxicity was investigated. Thirty seven Sprague Dawley female rats were randomly divided into four groups. The groups include control group, edaravone (30 mg–1 kg–1 day–1) given group, VPA (0.5 g–1 kg–1 day–1) given group, VPA + edaravone (in same dose) given group. Edaravone and VPA were given intraperitoneally for 7 days. Biochemical parameters such as malondialdehyde, as an index of lipid peroxidation(LPO), sialic acid (SA), glutathione levels and glutathione peroxidase, glutathione- S-transferase, superoxide dismutase, catalase, myeloperoxidase, alkaline phosphatase (ALP), and tissue factor (TF) activities were determined in small intestine samples by colorimetric methods. Decreased small intestine antioxidant enzyme activities, increased LPO and SA levels, and increased activities of ALP and TF were detected in the VPA group. Based on our results edaravone may be suggested to reverse the oxidative stress and inflammation due to VPA-induced small intestine toxicity.

Effect of Turmeric and Ginger on Lipid Profile in Male Rats Exposed to Oxidative Stress

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Eman A. Al-Rekabi ◽  
Dheyaa K. Alomer ◽  
Rana Talib Al-Muswie ◽  
Khalid G. Al-Fartosi
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Drinking Water ◽  
Lipid Profile ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Male Rats ◽  
Control Group ◽  
Very Low Density Lipoprotein ◽  
Low Density

The present study aimed to investigate the effect of turmeric and ginger on lipid profile of male rats exposed to oxidative stress induced by hydrogen peroxide H2O2 at a concentration of 1% given with consumed drinking water to male rats. Methods: 200 mg/kg from turmeric and ginger were used, and the animals were treatment for 30 days. Results: the results showed a significant increase in cholesterol, triglycerides, low density lipoprotein (LDL), very low density lipoprotein (VLDL), whereas it explained a significant decrease in high density lipoprotein (HDL) of male rats exposed to oxidative stress when compared with control group. the results showed a significant decrease in cholesterol, triglycerides, (LDL), (VLDL), whereas it explained a significant increase in (HDL) of rats treated with turmeric and ginger at dose 200 mg/kg when compared with male rats exposed to oxidative stress.

UJI AKTIVITAS HEPATOPROTEKTOR KOMBINASI EKSTRAK ETANOL DAUN PANDAN WANGI (Pandanus amaryllifolius roxb) DAN KAYU MANIS (Cinnamomum burmanii) TERHADAP KADAR MDA YANG DIINDUKSI PARASETAMOL

2020 ◽  
pp. 68-73
Author(s):  
Yuni Asri Mulatsih Agami ◽  
Eka Wisnu Kusuma
Keyword(s):  
Oxidative Stress ◽  
Ethanol Extract ◽  
Optimal Dose ◽  
Experimental Methods ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Negative Group ◽  
Ic50 Value ◽  
Dose Combination

Kasus penyakit hati semakin meningkat seiring penggunaan senyawa hepatotoksin salah satunya karena penggunaan parasetamol dengan dosis berlebih. Hal tersebut dapat meningkatkan produksi radikal bebas sehingga memicu terjadinya stress oksidatif yang dapat menimbulkan kerusakan jaringan yang ditandai dengan peningkatan kadar Malondialdehyde (MDA). Stress oksidatif dapat diatasi dengan antioksidan dari berbagai tanaman. Kulit kayu manis memiliki aktivitas antioksidan dengan nilai IC50 53ppm dan daun pandan wangi 39,7%  Penelitian ini bertujuan untuk mengetahui aktivitas kombinasi ekstrak etanol daun pandan wangi dan kayu manis dalam menurunkan kadar MDA. tikus yang diinduksi parasetamol. Penelitian menggunakan metode eksperimental, dilakukan selama 9 hari dengan 30 ekor tikus jantan dibagi menjadi 6 Kelompok, yaitu: Normal diberi aquadest, Kontrol Positif diberi silimarin 100 mg/kgBB, Kontrol Negatif diberi CMC-Na 0,05%, serta 3 kelompok lainnya diberi kombinasi ekstrak daun pandan wangi:kayu manis berturut-turut dosis I (25:75), dosis II (50:50), dosis III (75:25). Semua kelompok diinduksi parasetamol 2,5 g/kgBB pada hari ke-7  setelah 30 menit perlakuan, kecuali kelompok normal. Pada hari ke 9 dilakukan pengukuran kadar MDA dengan metode TBARs menggunakan spektrofotometri. Pemberian kombinasi ekstrak etanol daun pandan wangi dan kayu manis dapat menurunkan kadar MDA dengan kombinasi dosis yang paling optimal adalah 75:25 berdasarkan statistik dengan nilai signifikan 0,000<0,05 dibandingkan dengan kelompok negatif.    Cases of liver disease have increased with the use of hepatotoxin compounds, one of which is due to the use of paracetamol with excessive doses. This can increase the production of free radicals so that it triggers oxidative stress which can cause tissue damage which is characterized by increased levels of Malondialdehyde (MDA). Oxidative stress can be overcome with antioxidants from various plants. Cinnamomum burmanii has antioxidant activity with IC50 value of 53ppm and Pandanus amarrylifolius 39.7%. This study aims to determine the combined activity of ethanol extract of Pandanus amarrylifolius and Cinnamomum burmanii  in reducing MDA levels. Paracetamol-induced rats. Research using experimental methods, conducted for 9 days with 30 male rats divided into 6 groups, namely: Normal given aquadest, Positive Control were given silimarin 100 mg / kgBB, Negative Control was given CMC-Na 0.05%, and 3 other groups were given a combination of Pandanus amarrylifolius extract: Cinnamomum burmanii dose I (25:75), dose II (50:50), dose III (75:25). All groups induced paracetamol 2.5 g / kgBB on the 7th day after 30 minutes of treatment, except the normal group. On the 9th day MDA levels were measured using the TBARs method using spectrophotometry. Giving a combination of Pandanus amarrylifolius and Cinnamomum burmanii ethanol extract can reduce MDA levels with the most optimal dose combination is 75:25 based on statistics with a significant value of 0,000<0.05 compared with the negative group.

Effect of Opuntia dejecta (Salm-Dyck) flowers in liver of fructose-fed rats: Biochemicals, enzymatic and histological studies

2021 ◽  
pp. 096032712110134
Author(s):  
O Zouaoui ◽  
K Adouni ◽  
A Jelled ◽  
A Thouri ◽  
A Ben Chrifa ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Body Weight ◽  
Diabetes Type 2 ◽  
Male Rats ◽  
Control Group ◽  
Standard Drug ◽  
High Fructose ◽  
Group A ◽  
Histological Architecture

Phytochemical composition and antioxidant activity of flowers decoction at post-flowering stage (F3D) of Opuntia dejecta were determined. The obtained findings demonstrate that F3D has a marked antioxidant activity in all tested assays. Furthermore, the present study was designed to test the protective activity of F3D against induced Diabetes type 2 (DT2) in male rats. Those metabolic syndromes were induced by a high-fructose diet (HFD) (10% fructose solution) for a period of 20 weeks. F3D was administered orally (100 and 300 mg/kg body weight) daily for the last 4 weeks. Metformin (150 mg/kg body weight) was used as a standard drug and administrated orally for the last 4 weeks. The results showed a significant increase in blood glucose, triglycerides and hepatic markers (ALAT, ASAT and ALK-P) in HFD group. A significant increase in hepatic TBARS and a significant decrease in SOD, CAT and GPX were observed in fructose fed rats compared to control group. Administration of F3D showed a protective effect in biochemical and oxidative stress parameters measured in this study. Also, oral administration of F3D restored the histological architecture of rat liver in comparison with rats fed HFD. In conclusion, F3D attenuated hepatic oxidative stress in fructose-fed rats.

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