Impact of Transfaunation of Rumen Ciliate Cultures on Physical Examination, Selected Serum Parameters and Milk Profile in Defaunated Lactating Dairy Goats

2020 ◽  
Keyword(s):  
Physical Examination ◽  
Mixed Culture ◽  
Pure Culture ◽  
Milk Fat ◽  
Mixed Cultures ◽  
Dairy Goats ◽  
Serum Parameters ◽  
Native Breed ◽  
Culture Duration ◽  
Inorganic Phosphorous

Rumen ciliates still have mysterious secrets and influences in ruminants. This study investigated the effect of transfaunation of pure and mixed cultures of rumen ciliates on physical clinical examination, selected serum parameters and milk profile in defaunated lactating dairy goats. A number of 8 Baladi native breed goats were randomly classified into two groups each one containing 4 goats. Pure culture group was transfaunated with 6 ml of pure culture of Holotricha spp., while mixed culture group was transfaunated with 6 ml of mixed culture of 81.85% Holotricha and 18.15% Ophryoscolex spp. once weekly for three consecutive weeks, after defaunation of both groups using 30 ml of 8% SLS for two consecutive days. Serum and milk samples were collected weekly for three successive weeks to study effect of type of ciliate culture, duration of transfaunation and their interaction. Results revealed that transfaunation of pure and mixed cultures of rumen ciliates had no effect on physical examination with minimal non-significant improvement of calcium, inorganic phosphorous, total protein and globulin in serum of defaunated goats. Transfaunation of pure or mixed cultures of rumen ciliates within three weeks could not improve significantly decreased milk fat % of defaunated goats without any effect on other measured milk profile parameters. It is concluded that further investigations on transfaunation without prior defaunation should be performed using different pure and mixed cultures of rumen ciliates for therapeutic and productive purposes.

scholarly journals Enhanced Removal of a Pesticides Mixture by Single Cultures and Consortia of Free and ImmobilizedStreptomycesStrains

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
María S. Fuentes ◽  
Gabriela E. Briceño ◽  
Juliana M. Saez ◽  
Claudia S. Benimeli ◽  
María C. Diez ◽  
...  
Keyword(s):  
Mixed Culture ◽  
Pure Culture ◽  
Immobilized Cells ◽  
Mixed Cultures ◽  
Ex Situ ◽  
Antagonistic Effects ◽  
Pure Cultures

Pesticides are normally used to control specific pests and to increase the productivity in crops; as a result, soils are contaminated with mixtures of pesticides. In this work, the ability ofStreptomycesstrains (either as pure or mixed cultures) to remove pentachlorophenol and chlorpyrifos was studied. The antagonism among the strains and their tolerance to the toxic mixture was evaluated. Results revealed that the strains did not have any antagonistic effects and showed tolerance against the pesticides mixture. In fact, the growth of mixed cultures was significantly higher than in pure cultures. Moreover, a pure culture (Streptomycessp. A5) and a quadruple culture had the highest pentachlorophenol removal percentages (10.6% and 10.1%, resp.), whileStreptomycessp. M7 presented the best chlorpyrifos removal (99.2%). Mixed culture of allStreptomycesspp. when assayed either as free or immobilized cells showed chlorpyrifos removal percentages of 40.17% and 71.05%, respectively, and for pentachlorophenol 5.24% and 14.72%, respectively, suggesting better removal of both pesticides by using immobilized cells. These results reveal that environments contaminated with mixtures of xenobiotics could be successfully cleaned up by using either free or immobilized cultures ofStreptomyces, throughin situorex situremediation techniques.

Plaque Formation by Mixed Cultures of Nonprimary Plaque-Forming Microorganisms

1974 ◽  
Vol 53 (4) ◽  
pp. 825-831 ◽  
Author(s):  
John C. Parsons ◽  
Chris H. Miller
Keyword(s):  
Mixed Culture ◽  
Pure Culture ◽  
Plaque Formation ◽  
Mixed Cultures ◽  
Streptococcus Salivarius

Some oral organisms that do not form artificial plaque in pure culture interacted to form adherent plaque when combined in mixed culture. The presence of a nonprimary plaque-forming strain of Streptococcus salivarius in combinations with one of several other nonprimary plaque formers, resulted in depositions of large quantities of plaque containing significant amounts of a fructose-positive polysaccharide.

0870 Percentages of milk fat, lactose, and protein are affected by diurnal variations in dairy goats

2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 418-418
Author(s):  
F. Rosa ◽  
J. S. Osorio ◽  
J. Lohakare ◽  
M. Moridi ◽  
A. Ferrari ◽  
...  
Keyword(s):  
Milk Fat ◽  
Diurnal Variations ◽  
Dairy Goats

scholarly journals PSX-8 Effects of dietary concentrate level and limited feed access on feed intake and performance by lactating Alpine dairy goats

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 455-456
Author(s):  
Luana P Ribeiro ◽  
Ryszard Puchala ◽  
Terry A Gipson ◽  
Raquel V Lourencon ◽  
Arthur L Goetsch
Keyword(s):  
Feed Intake ◽  
Milk Fat ◽  
Protein Concentration ◽  
Free Choice ◽  
Energy Yield ◽  
Dairy Goats ◽  
Improve Performance ◽  
Entire Study ◽  
And Performance ◽  
Concentrate Level

Abstract The objective was to determine if effects of different limited feed access treatments on performance by lactating Alpine dairy goats vary with dietary concentrate level. Primiparous (29; 55.2±0.8 kg BW) and multiparous Alpine goats (29; 70.2±1.06 kg) were used in a 24-wk study of 3 8-wk phases beginning at 7.1±0.37 d after kidding. Diets of 40 or 60% concentrate (40C and 60C, respectively) were offered free choice in Calan gate feeders, with access continuously (CON), for 8 h between morning and afternoon milkings (DAY), or for 2 h after each milking (FRH). Although there were many interactions involving phase, ADG during the entire study was greater for CON vs. FRH (P < 0.05; -20, -41, and -61 g for CON, DAY, and FRH, respectively; SEM = 8.6). Intake of DM also was greater for CON than for FRH (P < 0.05; 2.17, 2.03, 2.01, 2.29, 2.07, and 1.80 kg/d for 40C-CON, 40C-DAY, 40C-FRH, 60C-CON, 60C-DAY, and 60C-FRH, respectively; SEM=0.093). There were interactions (P < 0.02) between concentrate level and access treatment in milk yield (2.55, 2.41, 2.61, 3.21, 2.66, and 2.27 kg/d; SEM=0.103) and protein concentration (2.65, 2.55, 2.60, 2.67, 2.70, and 2.49%; SEM = 0.042), and milk fat concentration was greatest among treatments (P < 0.05) for FRH (2.79, 2.77, 3.18, 2.86, 2.89, and 3.02% for 40C-CON, 40C-DAY, 40C-FRH, 60C-CON, 60C-DAY, and 60C-FRH, respectively; SEM=0.103). As a consequence, milk energy yield was 6.14, 5.70, 6.67, 7.83, 6.58, and 5.61 MJ/d (SEM=0.251) and the ratio of milk energy to DMI was 3.01, 2.87, 3.37, 3.47, 3.28, and 3.18 MJ/kg for 40C-CON, 40C-DAY, 40C-FRH, 60C-CON, 60C-DAY, and 60C-FRH, respectively; SEM=0.123). Overall, limited feeder access treatments did not improve performance and, in fact, the shortest access treatment increased BW loss; however, efficiency of feed DM usage for milk energy yield was not adversely impacted.

scholarly journals Methods to extract the exopolymeric matrix from biofilms: a comparative study

1999 ◽  
Vol 39 (7) ◽  
pp. 243-250 ◽  
Author(s):  
Joana Azeredo ◽  
Valentina Lazarova ◽  
Rosário Oliveira
Keyword(s):  
Organic Carbon ◽  
Comparative Study ◽  
Mixed Culture ◽  
Pure Culture ◽  
Cell Walls ◽  
Extraction Method ◽  
Extraction Methods ◽  
Suitable Method ◽  
The Matrix ◽  
Biofilm Matrix

To study the composition of a biofilm a previous extraction method is required to separate cells from the matrix. There are several methods reported in the literature; however they are not efficient or promote leakage of intracellular material. In this work several extraction methods were assayed in mixed culture and pure culture biofilms and their efficiency was evaluated by the amount of organic carbon, proteins and intracellular material extracted. The results showed that the extraction with glutaraldehyde 3% (w/v) was the most suitable method, extracting great amounts of organic carbon without promoting cell lysis or permeabilization. Glutaraldehyde is a bifunctional reagent that binds to cell walls avoiding their permeabilization and the biofilm matrix is solubilized in the solution.

Note on the effect of temperature on a mixed culture of two organisms in symbiotic relation

1939 ◽  
Vol 29 (2) ◽  
pp. 302-305 ◽  
Author(s):  
E. H. Richards
Keyword(s):  
Nitrogen Fixation ◽  
Mixed Culture ◽  
Azotobacter Chroococcum ◽  
Mixed Cultures ◽  
Effect Of Temperature ◽  
Two Temperatures ◽  
Symbiotic Relation

1. A study was made of nitrogen-fixation byAzotobacter chroococcumalone in a medium containing dextrose (which it can utilize) and in mixture with a coliform organism on a medium containing no carbohydrate except starch, whichAzotobactercannot utilize unless it be hydrolysed by the coliform organism or some other agency.2. The amount of nitrogen fixed in the mixed cultures was found to be maximal at two temperatures, and a discussion is given of the causes thought to be operative in producing the double maximum.

scholarly journals Effects of dietary energy levels using calcium salts of fatty acids on nutritive value of diets and milk quality in peripartum dairy goats

2014 ◽  
Vol 38 (3) ◽  
pp. 286-294 ◽  
Author(s):  
Rodrigo de Souza ◽  
Claudete Regina Alcalde ◽  
Bruna Hygino ◽  
Bruna Susan de Labio Molina ◽  
Geraldo Tadeu dos Santos ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Body Weight ◽  
Energy Level ◽  
Milk Fat ◽  
Dry Matter ◽  
Nutritive Value ◽  
Energy Levels ◽  
Milk Quality ◽  
Dietary Energy ◽  
Dairy Goats

Peripartum is a critical period for dairy goats, as dry matter intake (DMI) is not enough to supply the energy requirement for foetal growth and subsequent lactation. The objective of this study was to evaluate the use of calcium salts of fatty acids (CSFA) to increase dietary energy level of peripartum dairy goats and its effects on nutritive value of diets and milk quality. Twenty multiparous Saanen goats (body weight 63.5±10.3 kg) were distributed in a completely randomised design in four treatments at the following dietary energy levels: 2.6 Mcal of metabolisable energy per kg of dry matter (Mcal ME/kg DM) - control diet; and 2.7, 2.8, and 2.9 Mcal/kg DM - with added CSFA. Goats were housed in individual stalls and evaluated in the peripartum period, by measuring body weight (BW), DMI, dry matter and nutrient digestibility of diets, blood composition, and milk quality and yield. Increasing dietary energy level to 2.9 Mcal ME/kg DM had no effect on BW and did not limit DMI. CSFA supplementation increased intake of total digestible nutrients and did not affect fibre digestion. Blood triglycerides and cholesterol concentration increased with CSFA addition. The treatments had no effect on milk yield and composition; however, CSFA supplementation changed the fatty acid concentration of milk fat, increasing levels of polyunsaturated and essential fatty acids. In conclusion, peripartum dairy goats supplemented with CSFA to increase dietary energy level up to 2.9 Mcal ME/kg DM had a greater supply of total digestible nutrients and showed altered fatty acid concentration of milk fat.

Experimentelle Rotlauf- und Streptokokken-Endokarditis bei der Ratte

1971 ◽  
Vol 8 (2) ◽  
pp. 146-174 ◽  
Author(s):  
A. Kast ◽  
M. Herbst ◽  
L. Stoll
Keyword(s):  
Endothelial Cells ◽  
Mixed Culture ◽  
Bacterial Endocarditis ◽  
Mixed Cultures ◽  
The Other ◽  
Male Rats ◽  
Bacterial Colonisation ◽  
Intravenous Injections ◽  
Single And Mixed Cultures

The ability of intravenous injections of single and mixed cultures of streptococci of types A, C, D, E, G, and N, of S. viridans and of E. rhusiopathiae of serotypes A and B, to cause endocarditis in rats was tested. A mixed culture of three strains of Erysipelothrix was particularly effective, especially in male rats, whereas in all the other experiments, bacterial endocarditis seldom occurred. The endocarditis was a consequence of vasculitis and myocarditis. In most cases of valvular endocarditis subendothelial edema caused detachment of endothelial cells and provided the environment for bacterial colonisation of the valves.

Determination of Kinetic Constants of Activated Sludge Microorganisms

1985 ◽  
Vol 17 (2-3) ◽  
pp. 259-272 ◽  
Author(s):  
J. S. Čech ◽  
J. Chudoba ◽  
P. Grau
Keyword(s):  
Activated Sludge ◽  
Mixed Culture ◽  
Removal Rate ◽  
Mixed Cultures ◽  
Kinetic Constants ◽  
Type Reactor ◽  
Substrate Removal ◽  
Velocity Coefficient ◽  
Mixed Reactor

A respirometric method for measuring kinetic constants of activated sludge microorganisms by means of a simple respirometer was developed and tested by using two types of mixed culture. It has been found that both the maximum substrate removal rate and the half-velocity coefficient are basically lower with the mixed cultures cultivated in a completely-mixed reactor /filamentous/ than with those cultivated in a selector-type reactor /nonfilamentous/.

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