STUDY OF THE POSSIBILITY OF USING ULTRASOUND TO INTENSIFY EXTRACTION METHODS

2021 ◽  
pp. 180-186
Author(s):  
Anna Anatolyevna Chakhirova ◽  
Marina Alekseevna Ogai ◽  
Elena Vladimirovna Kovtun ◽  
Larisa Petrovna Lezhneva ◽  
Mikhail Viktorovich Kovtun
Keyword(s):  
Extraction Methods

The effect of different extraction methods on chemical composition profile and pharmacological activities of essential oils and hydrosols

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
F Ghavidel ◽  
MM Zarshenas ◽  
A Sakhteman ◽  
A Gholami ◽  
Y Ghasemi ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oils ◽  
Extraction Methods ◽  
Composition Profile ◽  
Pharmacological Activities

Characterization of dissolved organic matter (DOM) extracted from soils by hot water percolation (HWP)

2010 ◽  
Vol 59 (1) ◽  
pp. 99-108 ◽  
Author(s):  
M. Takács ◽  
Gy. Füleky
Keyword(s):  
Organic Matter ◽  
Humic Acid ◽  
Natural Organic Matter ◽  
Extraction Methods ◽  
Soil Samples ◽  
Hot Water ◽  
Soil Extracts ◽  
Water Percolation ◽  
Soil Humic Acid

The Hot Water Percolation (HWP) technique for preparing soil extracts has several advantages: it is easily carried out, fast, and several parameters can be measured from the same solution. The object of this study was to examine the possible use of HWP extracts for the characterization of soil organic matter. The HPLC-SEC chromatograms, UV-VIS and fluorescence properties of the HWP extracts were studied and the results were compared with those of the International Humic Substances Society (IHSS) Soil Humic Acid (HA), IHSS Soil Fulvic Acid (FA) and IHSS Suwannee Natural Organic Matter (NOM) standards as well as their HA counterparts isolated by traditional extraction methods from the original soil samples. The DOM of the HWP solution is probably a mixture of organic materials, which have some characteristics similar to the Soil FA fractions and NOM. The HWP extracted organic material can be studied and characterized using simple techniques, like UV-VIS and fluorescence spectroscopy.

New Feature Vectors using GFCC for Speaker Identification

2018 ◽  
Vol 6 (8) ◽  
pp. 243
Author(s):  
A. Nagesh
Keyword(s):  
Speaker Recognition ◽  
Speaker Identification ◽  
Signal To Noise Ratio ◽  
Main Idea ◽  
Extraction Methods ◽  
Identification System ◽  
Identification Performance ◽  
Feature Vectors ◽  
Overall Performance ◽  
New Feature

The feature vectors of speaker identification system plays a crucial role in the overall performance of the system. There are many new feature vectors extraction methods based on MFCC, but ultimately we want to maximize the performance of SID system.  The objective of this paper to derive Gammatone Frequency Cepstral Coefficients (GFCC) based a new set of feature vectors using Gaussian Mixer model (GMM) for speaker identification. The MFCC are the default feature vectors for speaker recognition, but they are not very robust at the presence of additive noise. The GFCC features in recent studies have shown very good robustness against noise and acoustic change. The main idea is  GFCC features based on GMM feature extraction is to improve the overall speaker identification performance in low signal to noise ratio (SNR) conditions.

A Review of Different Techniques of Age Estimation from Human Face

2018 ◽  
Vol 4 (10) ◽  
pp. 6
Author(s):  
Khemchandra Patel ◽  
Dr. Kamlesh Namdev
Keyword(s):  
Age Estimation ◽  
Real Life ◽  
Extraction Methods ◽  
Human Face ◽  
Age Changes ◽  
Missing Children ◽  
Different Types ◽  
Age Variation ◽  
Human Faces ◽  
Facial Age

Age changes cause major variations in the appearance of human faces. Due to many lifestyle factors, it is difficult to precisely predict how individuals may look with advancing years or how they looked with "retreating" years. This paper is a review of age variation methods and techniques, which is useful to capture wanted fugitives, finding missing children, updating employee databases, enhance powerful visual effect in film, television, gaming field. Currently there are many different methods available for age variation. Each has their own advantages and purpose. Because of its real life applications, researchers have shown great interest in automatic facial age estimation. In this paper, different age variation methods with their prospects are reviewed. This paper highlights latest methodologies and feature extraction methods used by researchers to estimate age. Different types of classifiers used in this domain have also been discussed.

OPTIMIZATION OF DNA EXTRACTION METHODS FROM BIOLOGICAL MATERIALS OF HONEY BEES IN A DIFFERENT METAMOTPHOSIS PHASE

2016 ◽  
Vol 52 ◽  
pp. 171-176
Author(s):  
M. Palkina ◽  
O. Metlitska
Keyword(s):  
Ion Exchange ◽  
Dna Extraction ◽  
Honey Bees ◽  
Biological Material ◽  
Exchange Resin ◽  
Ion Exchange Resin ◽  
Extraction Methods ◽  
Chelex 100 ◽  
Drone Brood ◽  
Dna Extraction Methods

The aim of the research – adaptation, optimization and using of existing DNA extraction methods from bees’ biological material with the reagent «Chelex-100" under complex economic conditions of native laboratories, which will optimize labour costs and improve the economic performance of DNA extraction protocol. Materials and methods. In order to conduct the research the samples of honey bees’ biological material: queen pupae exuviae, larvae of drone brood, some adult bees’ bodies (head and thorax) were selected. Bowl and drone brood were obtained from the experimental bee hives of Institute of Apiculture nd. a. P. I. Prokopovich of NAAS. DNA extraction from biosamples of Apis mellifera ssp. was carried out using «Chelex-100®» ion exchange resin in different concentrations and combinations. Before setting tests for determination of quantitative and quality indexes, dilution of DNA samples of the probed object was conducted in ratio 1:40. The degree of contamination with protein and polysaccharide fractions (OD 260/230), quantitative content of DNA (OD 260/280) in the extracted tests were conducted using spectrophotometer of «Biospec – nano» at the terms of sample volume in 2 µl and length of optical way in 0,7 mm [7]. Verification of DNA samples from biological material of bees, isolated by «Chelex-100®», was conducted after cold keeping during 24 hours at 20°C using PСR with primaries to the fragment of gene of quantitative trait locus (QTL) Sting-2 of next structure [8]:  3' – CTC GAC GAG ACG ACC AAC TTG – 5’; 3' – AAC CAG AGT ATC GCG AGT GTT AC – 5’ Program of amplification: 94 °C – 5 minutes – 1 cycle; 94 °C – 1 minute, 57°C – 1 minute, 72 °C – 2 minutes – 30 cycles; elongation after 72°C during 2 minutes – 1 cycle. The division of obtained amplicons was conducted by gel electrophoresis at a low current – 7 µÀ, in 1,5 % agarose gel (Sigma ®) in TAE buffer [7]. The results. At the time of optimization of DNA isolation methods, according to existing methods of foreign experts, it was found optimal volume of ion exchange resin solution was in the proposed concentration: instead of 60 µl of solution used 120 µl of «Chelex-100®», time of incubation was also amended from 30 minutes to 180 minutes [9]. The use of the author's combination of method «Chelex-100®» with lysis enzymes, proteinase K and detergents (1M dithiothreitol), as time of incubation was also amended, which was reduced to 180 minutes instead of the proposed 12 hours [10]. Changes in quality characteristics of obtained DNA in samples after reduction in incubation time were not found. Conclusions. The most economical method of DNA isolation from bees’ biological material is 20% solution of «Chelex-100» ion exchange resin with the duration of the incubation period of 180 minutes. It should also be noted that the best results can be obtained from exuviae, selected immediately after the queen’s exit from bowl, that reduces the likelihood of DNA molecules destruction under the influence of nucleases activation, but not later than 12 hours from release using the technology of isolated obtain of queens.

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