scholarly journals Post-mortem Gross Detection of Early Myocardial Infarction using Triphenyl Tetrazolium Chloride Test

2021 ◽  
Keyword(s):  
Myocardial Infarction ◽  
Post Mortem ◽  
Triphenyl Tetrazolium Chloride ◽  
Tetrazolium Chloride

scholarly journals Potential Fertilization Capacity of Two Grapevine Varieties: Effects on Agricultural Production in Designation of Origin Areas in the Northwestern Iberian Peninsula

Agronomy ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 961
Author(s):  
J. Antonio Cortiñas Rodríguez ◽  
María Fernández-González ◽  
Estefanía González-Fernández ◽  
Rosa A. Vázquez-Ruiz ◽  
F. Javier Rodríguez-Rajo ◽  
...  
Keyword(s):  
Airborne Pollen ◽  
Germination Rate ◽  
Pollen Viability ◽  
Weather Conditions ◽  
Triphenyl Tetrazolium Chloride ◽  
Tetrazolium Chloride ◽  
The Mean ◽  
Study Sites ◽  
Fertilization Capacity

In the present study, we analyzed the main parameters related with the potential fertilization ability of two grapevine varieties, Godello and Mencía, during the years 2017 and 2018. The research was carried out in two vineyards of the Galician winegrowing Designation of Origin areas of Ribeiro and Ribeira Sacra. Ten vines of each variety were selected for bunch and flower counting, pollen calculations, pollen viability studies by means of aceto-carmine (AC) stain and 2, 3, 5-triphenyl tetrazolium chloride (TTC) methods, and the determination of their germination rate. In all vineyards the 50% fruitset was reached, except for Godello in Cenlle during 2017. The mean coulure value was higher for Godello (40.5%) than for Mencía (31%). Analyzing the pollen production per plant and airborne pollen levels, we observed important discordances between them, which can be due to the influence of weather conditions and be related with self-pollination processes. We found important differences on pollen viability depending on the applied method and variety, with higher values for the AC method than the TTC for both varieties in all study plots, and higher values for Mencía variety than Godello. Regarding germination rates, we observed a marked reduction in 2017 with respect to 2018, in all study sites and for both varieties. The analyzed parameters were useful to explain the different productive abilities of Godello and Mencía varieties in the two studied bioclimatic regions of Ribeiro and Ribeira Sacra.

scholarly journals Endothelial β1 Integrin-Mediated Adaptation to Myocardial Ischemia

2021 ◽  
Author(s):  
Carina Henning ◽  
Anna Branopolski ◽  
Paula Follert ◽  
Oksana Lewandowska ◽  
Aysel Ayhan ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Coronary Artery ◽  
Myocardial Ischemia ◽  
Cardiac Function ◽  
Integrin Subunit ◽  
Β1 Integrin ◽  
Human Coronary Artery ◽  
Tetrazolium Chloride ◽  
Magnetic Resonance Imaging Mri ◽  
Interfering Rna

Abstract Background Short episodes of myocardial ischemia can protect from myocardial infarction. However, the role of endothelial β1 integrin in these cardioprotective ischemic events is largely unknown. Objective In this study we investigated whether endothelial β1 integrin is required for cardiac adaptation to ischemia and protection from myocardial infarction. Methods Here we introduced transient and permanent left anterior descending artery (LAD) occlusions in mice. We inhibited β1 integrin by intravenous injection of function-blocking antibodies and tamoxifen-induced endothelial cell (EC)-specific deletion of Itgb1. Furthermore, human ITGB1 was silenced in primary human coronary artery ECs using small interfering RNA. We analyzed the numbers of proliferating ECs and arterioles by immunohistochemistry, determined infarct size by magnetic resonance imaging (MRI) and triphenyl tetrazolium chloride staining, and analyzed cardiac function by MRI and echocardiography. Results Transient LAD occlusions were found to increase EC proliferation and arteriole formation in the entire myocardium. These effects required β1 integrin on ECs, except for arteriole formation in the ischemic part of the myocardium. Furthermore, this integrin subunit was also relevant for basal and mechanically induced proliferation of human coronary artery ECs. Notably, β1 integrin was needed for cardioprotection induced by transient LAD occlusions, and the absence of endothelial β1 integrin resulted in impaired growth of blood vessels into the infarcted myocardium and reduced cardiac function after permanent LAD occlusion. Conclusion We showed that endothelial β1 integrin is required for adaptation of the heart to cardiac ischemia and protection from myocardial infarction.

scholarly journals Post-mortem cardiac diffusion tensor imaging: detection of myocardial infarction and remodeling of myofiber architecture

2014 ◽  
Vol 24 (11) ◽  
pp. 2810-2818 ◽  
Author(s):  
Sebastian Winklhofer ◽  
Christian T. Stoeck ◽  
Nicole Berger ◽  
Michael Thali ◽  
Robert Manka ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Diffusion Tensor Imaging ◽  
Diffusion Tensor ◽  
Post Mortem ◽  
Imaging Detection

A Histochemical Study of Dehydrogenase Activity in the Pectoralis Major Muscle of the Pigeon and certain other Vertebrate Skeletal Muscles

1958 ◽  
Vol s3-99 (48) ◽  
pp. 469-473
Author(s):  
J. C. GEORGE ◽  
K. S. SCARIA
Keyword(s):  
Dehydrogenase Activity ◽  
Skeletal Muscles ◽  
Pectoralis Major Muscle ◽  
Breast Muscle ◽  
Muscle Fibres ◽  
Triphenyl Tetrazolium Chloride ◽  
Tetrazolium Chloride ◽  
Unique System ◽  
Oxidative Processes ◽  
The Individual

Certain dehydrogenases in the breast muscle of the pigeon and fowl and the leg muscle of the fowl and frog were studied histochemically by the use of 2:3:5: triphenyl tetrazolium chloride. The dehydrogenase activity was found to have a relationship with the colour and the mitochondrial content of the individual muscle fibres. In the pigeon breast muscle, however, the broad white fibres did not show the presence of any of the enzymes studied. It is therefore concluded that these fibres in the pigeon breast muscle are a unique system in which none of the oxidative processes concerned takes place; they cannot be considered as analogous to the white fibres of the other muscles studied.

Oxygen uptake and tetrazolium reduction during skin cycle of the mouse

1965 ◽  
Vol 209 (1) ◽  
pp. 231-235 ◽  
Author(s):  
Ira M. Dushoff ◽  
John Payne ◽  
Falls B. Hershey ◽  
Robert C. Donaldson
Keyword(s):  
Oxygen Consumption ◽  
Mouse Skin ◽  
Growth Cycle ◽  
Triphenyl Tetrazolium Chloride ◽  
Intact Skin ◽  
Tetrazolium Chloride ◽  
Quantitative Measurements ◽  
Close Relationship ◽  
Coefficient Of Regression ◽  
Tetrazolium Reduction

Quantitative measurements of oxygen consumption and triphenyl tetrazolium chloride reduction by intact skin were made during various stages of the skin cycle of the mouse. Values are highest during early anagen, when growth is most rapid: Qo2 is 2.47 ± 0.10 (mean ± se) and TTC is 537 ± 24.6. In late anagen the activities decrease 40–50%: Qo2 is 1.24 ± 0.10 and TTC is 315 ± 20.8. There is a close relationship between tetrazolium reduction and oxygen consumption during all phases of the growth cycle of mouse skin. The coefficient of regression of TTC on Qo2 is 163.2 ( P < .001).

Abstract 358: Myeloid-specific Il-4 Receptor α Knockout Alters Cardiac Remodeling Post-myocardial Infarction

2016 ◽  
Vol 36 (suppl_1) ◽  
Author(s):  
Jianrui Song ◽  
Thomas Vigil ◽  
Yutein Chung ◽  
Ryan Frieler ◽  
Sascha Goonewardena ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Infarct Size ◽  
Cardiac Remodeling ◽  
Post Myocardial Infarction ◽  
Critical Determinant ◽  
Tetrazolium Chloride ◽  
Alternatively Activated Macrophages ◽  
Inflammatory Reactions ◽  
Infarct Healing ◽  
Adverse Remodeling

Introduction: Cardiac remodeling post myocardial infarction (MI) can be a critical determinant of outcome for patients with MI. Well-contained inflammation results in successful infarct healing while excessive inflammation cause adverse remodeling which leads to heart failure. Macrophages are important participants in inflammation, helping resolve pro-inflammatory reactions and performing reparative processes. Reprogramming macrophages towards a resolving and reparative phenotype is a potential therapeutic approach. We hypothesized that IL4/IL13-induced, alternatively activated macrophages (M2) have an important role in cardiac remodeling post-MI, and we tested this hypothesis in a mouse model of MI using myeloid-specific IL4 receptor α knockout mice (MyIL4RaKO). Methods: MyIL4RaKO mice were generated using IL4Ra flox/flox ;LysM-Cre. MI was induced by ligating the left anterior descending coronary artery. Hearts were cut into 1mm sections, and then stained by tetrazolium chloride for infarct size measurement. Evenly spaced radians were taken through the infarct with the center of left ventricle in 5μm heart sections, and the average infarct thickness was calculated. qPCR was used to determine gene expression. Echocardiography was performed at baseline and 3 weeks post MI. Results: Initial infarct size was not affected by IL4Ra knockout but at 1-week post MI, infarct size of MyIL4RaKO mice (16.54 ± 2.433, n=11) was shown significantly smaller than that of FC mice (24.96 ± 2.005, n=15) showing changes in remodeling (p= 0.0129). Changes in remodeling continued and at 3-week post MI, infarct thickness of MyIL4RaKO mice (0.2171 ± 0.01053, n=6) was significantly increased, compared with that of FC mice (0.3508 ± 0.03629, n=8, p= 0.0094). These changes were accompanied by MyIL4RaKO mice also showed lower level of fibrosis markers: Col1A1 and Plod2. A significantly lower ejection fraction was observed in MyIL4RaKO mice (25.46 ± 3.749, n=5) compared with FC mice (37.90 ± 2.309, n=5) at 3 weeks (p= 0.0223). Conclusions: Myeloid-specific IL4Ra knockout results in alteration of remodeling, altered fibrosis and decreased cardiac function post MI, although the cardiac hypertrophy did not show significant change.

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