Frequency of Discordance of Diagnosis of Lymphoma by Histopathology alone versus Combined Histopathology and Immunohistochemistry

2017 ◽  
Vol 1 (01) ◽  
pp. 2-4
Author(s):  
Mohammed Ali ◽  
A K M Mynul Islam ◽  
Salina Haque ◽  
Md. Kamrul Hasan ◽  
Md. Hafizur Rahman ◽  
...  
Keyword(s):  
Hodgkin Lymphoma ◽  
Histopathological Examination ◽  
Final Diagnosis ◽  
Critical Issue ◽  
Cross Sectional Study ◽  
Suspected Case ◽  
Financial Constraint ◽  
Cross Sectional ◽  
Non Hodgkin Lymphoma ◽  
Appropriate Therapy

Background: Diagnosis of lymphoma is a critical issue in Bangladesh and still we have to rely on histopathology reports as limited number of centres doing immunohistochemistry (IHC) with panel of incomplete markers. Previously in most of the cases of lymphomas, IHC was not done due to financial constraint and lack of facilities. But IHC is essential for categorization according to WHO classification 2008, through which we can optimize appropriate therapy and predict outcome. Objective: To evaluate the discordance of diagnosis of lymphoma by combined histopathology and immunohistochemistry, and histopathology alone. Methodology: This is a comparative cross-sectional study of diagnoses of lymphoma by immunohistochemistry (IHC) in combination with histopathology of paraffin embedded tissue blocks with histopathology alone of suspected cases of lymphoma treated in the department of Haematology of National Institute of Cancer Research and Hospital (NICR&H) from May 2016 to November 2016. Preliminary histopathological examination was done on tissue biopsy in suspected case of lymphoma and subsequently IHC was done to know the discordance of initial diagnosis with final diagnosis after combined histopathology and IHC. Results: Out of 68 patients 57 (83.8%) were diagnosed as lymphoma on the basis of histopathology alone, among which 41 (60.3%) cases were diagnosed as Non-Hodgkin Lymphoma (NHL) and 16 (23.5%) cases as Hodgkin Lymphoma (HL).After combined histopathology and IHC 61 (89.7%) cases were confirmed as lymphoma of which 50 (73.5%0 were NHL and 11 (16.2%) were HL. Discordance of diagnosis was found in 18 (27%) cases between the two methods of diagnosis. Conclusion: Subjective variation in the accuracy of diagnosis of lymphoma on the basis of histopathology alone may occur. Inclusion of IHC with histopathology improves the accuracy of diagnosis of lymphoma.

STUDY OF HISTOPATHOLOGY IN PATIENTS WITH HODGKIN AND NON - HODGKIN LYMPHOMA

2016 ◽  
pp. 59-65 ◽  
Author(s):  
Van Mao Nguyen
Keyword(s):  
Lymph Node ◽  
Hodgkin Lymphoma ◽  
Cross Sectional Study ◽  
University Hospital ◽  
Cross Sectional ◽  
Histopathological Classification ◽  
Non Hodgkin Lymphoma ◽  
Key Words Lymphoma ◽  
Malignant Grade

Background: Lymphoma is one of the most ten common cancers in the world as well as in Vietnam which has been ever increasing. It was divided into 2 main groups Hodgkin and non – Hodgkin lymphoma in which non-Hodgkin lymphoma appeared more frequency, worse prognosis and different therapy. Objectives: - To describe some common characteristics in patients with non – Hodgkin lymphoma; - To determine the proportion between Hodgkin and non- Hodgkin lymphoma, histopathological classification of classical Hodgkin by modified Rye 1966 and non-Hodgkin lymphoma by Working Formulation (WF) of US national oncology institute 1982. Materials and Method: This cross-sectional study was conducted on 65 patients with Hodgkin and non- Hodgkin lymphoma diagnosed definitely by histopathology at Hue Central Hospital and Hue University Hospital. Results:. The ratio of male/female for the non-Hodgkin lymphoma was 1.14/1, the most frequent range of age was 51-60 accounting for 35%, not common under 40 years. Non - Hodgkin lymphoma appeared at lymph node was the most common (51.7%), at the extranodal site was rather high 48.3%. The non - Hodgkin lymphoma proportion was predominant 92.3% comparing to the Hodgkin lymphoma only 7.7%; The most WF type was WF7 (53.3%), following the WF6 18,3% and WF5 11,7%; The intermediate malignancy grade of non- Hodgkin lymphoma was the highest proportion accouting for 85%, then the low and the high one 8.3% and 6.7% respectively. Conclusion: The histopathological classification and the malignant grade of lymphoma for Hodgkin and non - Hodgkin lymphoma played a practical role for the prognosis and the treatment orientation, also a fundamental one for the modern classification of non - Hodgkin lymphoma nowadays. Key words: lymphoma, Hodgkin lymphoma, non-Hodgkin lymphoma, classication, grade, histopathology, lymph node

Niespecyficzny obraz chłoniaka B-komórkowego u dwuipółletniej dziewczynki – opis przypadku

2019 ◽  
Vol 24 (3) ◽  
Author(s):  
Ewa Krasuska-Sławińska ◽  
Izabela Królik-Elgas ◽  
Marzena Stypińska ◽  
Anna Matosek-Rutkowska
Keyword(s):  
B Cell ◽  
Hodgkin Lymphoma ◽  
Histopathological Examination ◽  
B Cell Lymphoma ◽  
Final Diagnosis ◽  
Lymphoblastic Lymphoma ◽  
Final Conclusion ◽  
Dental Surgery ◽  
Non Hodgkin Lymphoma ◽  
Primary Location

B-cell lymphoblastic lymphoma which is a type of non-Hodgkin lymphoma is rather uncommon in children. Focal changes in bones in the course of non-Hodgkin lymphoma are mostly secondary changes and their primal location in a bone is rare. PBL (primary bone lymphoma) mainly concerns a thighbone and a tibial bone; the primary location in jaw bones is quite sporadic. In diagnostics, there is mainly magnetic resonance, medical scan (tomography), and above all – histopathological test. There is also chemotherapy by choice, and primary location in a jaw or a mandible significantly advances the prognosis. The aim of the work is to introduce a patient who was definitively diagnosed B-cell lymphoblastic lymphoma from the early B-cells. The girl reported to Laryngological Clinic, Dental Surgery Clinic for Children, Oncological Clinic of Children’s Memorial Health Institute. The cause of the visit was an elevation on the right side of a nose base, present for two months and misdiagnosed by doctors as a post-traumatic swelling in this region. After introducing laboratory and scan diagnostics and taking biopsy from the lesion, a final conclusion was made. Also, a proper treatment according to the protocol for B-cell lymphoblastic lymphoma was introduced. Non-specific B-cell lymphoma picture, as mentioned in the described case, specifically due to location in a jaw bone and a slow pace of growing, may both constitute huge diagnostic problems and deteriorate prognosis. Therefore, it is important to take into account also lymphoma – in such location of a lesion. Moreover, it is worth remembering that the final diagnosis may only be passed on the basis of histopathological examination.

scholarly journals Long‐term fatigue in survivors of non‐Hodgkin lymphoma: The Lymphoma Study Association SIMONAL cross‐sectional study

Cancer ◽  
2019 ◽  
Author(s):  
Nicolas Mounier ◽  
Sabine Anthony ◽  
Raphaël Busson ◽  
Catherine Thieblemont ◽  
Vincent Ribrag ◽  
...  
Keyword(s):  
Hodgkin Lymphoma ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Non Hodgkin Lymphoma ◽  
Study Association

scholarly journals Comprehensive Geriatric Assessment in Elderly Patients with Aggressive Non-Hodgkin Lymphoma in Peru

Blood ◽  
2018 ◽  
Vol 132 (Supplement 1) ◽  
pp. 2307-2307
Author(s):  
Brady E. Beltrán ◽  
Rodrigo Motta ◽  
María P. Gamarra ◽  
Roger Araujo ◽  
Jorge J. Castillo
Keyword(s):  
Hodgkin Lymphoma ◽  
Geriatric Assessment ◽  
Comprehensive Geriatric Assessment ◽  
Research Funding ◽  
Cross Sectional Study ◽  
Bivariate Analysis ◽  
Geriatric Syndrome ◽  
Geriatric Syndromes ◽  
Cross Sectional ◽  
Non Hodgkin Lymphoma

Abstract Introduction: The incidence of Non-Hodgkin Lymphoma (NHL) is increasing, especially in people over 60 years of age. This population usually has a worse prognosis, probably due to comorbidities, functional deterioration and decreased tolerance to treatment. Therefore, a pre-therapeutic evaluation would be important to make decisions. The Comprehensive Geriatric Assessment (CGA) is the recommended tool for this evaluation, but it is a complex process that demands time and resources. We performed a study to evaluate the characteristics of the CGA in patients >64 years of age with NHL and to determine which domains can constitute a simplified model. Methods: This is a cross-sectional study with retrospective data collection of geriatric evaluations performed in >64 years with aggressive NHL (90% DLBCL, 10% PTCL) admitted to our institution between September 2015 and August 2017. Number of drugs, prescriptions in older adults was evaluated (STOPP), Lawton scale, Barthel scale, KATZ index, walking speed, Up and Go time (TUG), Mini-mental test, Yesavage scale, Gijón scale, Mini-nutritional, Geriatric syndromes (Incontinence, Falls, Pressure ulcers, Immobility, Sensory Deficits, Osteoporosis), and Accumulated Disease Scale in Geriatrics (CIRS-G). The CGA included 9 domains, and fragility was defined as deterioration in >2 domains. The different evaluations were compared with fragility. Multivariate models were constructed using logistic regression. Results: We included 253 patients with an average age of 76 years. 62% had >1 affected domain, and 40% were considered fragile (>2 affected domains). In the bivariate analysis, age >85 years, and all the geriatric scales except STOPP, were strongly associated with fragility. The final model had 6 variables: Use of >5 drugs ( OR 773.1, 95% CI,4.5-132134.4;p=0.011), Lawton scale <7 (OR 385.2,95%CI, 20.1-7363.5;p<0.001), TUG >20 (OR 124.6,95%CI, 6.2-2489.8;p=0.002), Mini-mental <23 (OR 53.5,95%CI, 3.7-778.8;p=0.004),Yesavage scale >5 (OR 118.7,95%CI, 11.2-1254.5;p<0.001), and presence of at least one geriatric syndrome (OR 235.6,95%CI, 13.3-4158.7;p<0.001). Removing the Mini-mental minimally affected the model, but suppressing two or more variables does weaken the model. Conclusions: In our cohort of patients older than 64 years with aggressive NHL, a model based on five measurements (i.e. number of drugs, TUG, Lawton scale, Yesavage scale and Geriatric Syndromes) could constitute an evaluation with an efficiency similar to a complete CGA. Disclosures Castillo: Pharmacyclics: Consultancy, Research Funding; Genentech: Consultancy; Millennium: Research Funding; Abbvie: Consultancy, Research Funding; Janssen: Consultancy, Research Funding; Beigene: Consultancy, Research Funding.

Economic burden in non‐Hodgkin lymphoma survivors: The French Lymphoma Study Association SIMONAL cross‐sectional study

Cancer ◽  
2021 ◽  
Author(s):  
Virginie Nerich ◽  
Christophe Guyeux ◽  
Michel Henry‐Amar ◽  
Raphaël Couturier ◽  
Catherine Thieblemont ◽  
...  
Keyword(s):  
Hodgkin Lymphoma ◽  
Economic Burden ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Non Hodgkin Lymphoma ◽  
Study Association ◽  
Lymphoma Survivors

Cognitive decline in older long-term survivors from Non-Hodgkin Lymphoma: a multicenter cross-sectional study

2020 ◽  
Vol 11 (5) ◽  
pp. 790-795 ◽  
Author(s):  
Domenico La Carpia ◽  
Rosa Liperoti ◽  
Mauro Guglielmo ◽  
Beatrice Di Capua ◽  
Liliana Franca Devizzi ◽  
...  
Keyword(s):  
Cognitive Decline ◽  
Hodgkin Lymphoma ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Non Hodgkin Lymphoma ◽  
Long Term Survivors

Bone Marrow and Peripheral Blood Evaluation in Non-Hodgkin Lymphoma – A Cross Sectional Study of 78 Cases in a Tertiary Care Centre in Kerala

2021 ◽  
Vol 8 (32) ◽  
pp. 2943-2949
Author(s):  
Arya Puthukkat Muraleedharan ◽  
Prabhalakshmy Kuzhikkattil Krishnankutty
Keyword(s):  
Bone Marrow ◽  
Hodgkin Lymphoma ◽  
Peripheral Blood ◽  
Bone Marrow Aspirate ◽  
Peripheral Blood Smear ◽  
Cross Sectional Study ◽  
Lymphoid Cells ◽  
Cross Sectional ◽  
Non Hodgkin Lymphoma ◽  
Blood Smears

BACKGROUND In the evaluation of patients with non-Hodgkin lymphoma (NHL), determination of bone marrow involvement is an integral part of staging work up. Peripheral blood counts and examination of blood smears are also done in patients with lymphoma as part of pre-treatment investigations. METHODS A cross sectional study of 78 patients with a prior histopathological diagnosis of NHL was conducted. Peripheral blood counts were performed on an automated haematology analyser to look for various cytopenias. Peripheral blood smears and bone marrow aspirate (BMA) / imprint smears were examined in detail for atypical lymphoid cells. Bone marrow trephine biopsies of these patients were studied to assess the NHL involvement and the various patterns of involvement. Adjuvant immunohistochemistry (IHC) was performed in bone marrow biopsies with scant cellularity or crush artefact to discern the marrow involvement. RESULTS Bone marrow trephine biopsy showed involvement by lymphoma in 65.4 % cases. The incidence of involvement was higher in B-cell lymphomas, especially in low grade types. The predominant pattern of involvement was interstitial pattern (41.2 %). Discordant histology between bone marrow and the primary anatomic site was found in 7.8 % of the cases, which was seen more in diffuse large B-cell lymphomas. Majority of the patients with bone marrow infiltration by NHL had anaemia (84.3 %). Bicytopenia and pancytopenia were also observed. On peripheral blood smear examination atypical lymphoid cells were present in 23 % cases. CONCLUSIONS Bone marrow examination is an important aspect in the diagnosis of NHL, because of its both prognostic and therapeutic implications. Hence, the presence of atypical lymphoid cells and other changes in the peripheral blood should be detected in these patients. KEYWORDS Non-Hodgkin Lymphoma, Bone Marrow Biopsy, Bone Marrow Aspirate / Imprint, Peripheral Blood Smear, Atypical Lymphoid Cells

Non-Hodgkin Lymphoma Diagnosis and Treatment in Kosovo, A Cross Sectional Study

2019 ◽  
Vol 19 ◽  
pp. S246
Author(s):  
Adriatik Berisha ◽  
Shemsedin Sadiku ◽  
Aferdita Ukimeraj ◽  
Shpat Rrusta ◽  
Edlira Gashi
Keyword(s):  
Hodgkin Lymphoma ◽  
Cross Sectional Study ◽  
Diagnosis And Treatment ◽  
Sectional Study ◽  
Cross Sectional ◽  
Non Hodgkin Lymphoma

scholarly journals Fatigue level changes with time in long-term Hodgkin and non-Hodgkin lymphoma survivors: a joint EORTC-LYSA cross-sectional study

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Raphaël Busson ◽  
Marleen van der Kaaij ◽  
Nicolas Mounier ◽  
Berthe M. P. Aleman ◽  
Catherine Thiéblemont ◽  
...  
Keyword(s):  
Hodgkin Lymphoma ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Non Hodgkin Lymphoma ◽  
Lymphoma Survivors

Histomorphologic and Immunohistochemical Study of Lymph-Node Biopsies in Generalised Lymphadenopathy

2021 ◽  
Vol 8 (07) ◽  
pp. 364-368
Author(s):  
Shayfa Palliyalil ◽  
Mini B
Keyword(s):  
Lymph Node ◽  
Hodgkin Lymphoma ◽  
Tertiary Care ◽  
Final Diagnosis ◽  
Cross Sectional Study ◽  
Age Group ◽  
Cross Sectional ◽  
Neoplastic Lesion ◽  
Neoplastic Lesions ◽  
Granulomatous Lymphadenitis

BACKGROUND “Lymphadenopathy’’ refers to nodes that are abnormal in size, consistency, or number. It is designated as generalised’’ if lymph-nodes are enlarged in two or more non-contiguous areas. Approximately three fourths of patients presenting with unexplained lymph-node enlargement have localised lymphadenopathy, whereas one fourth have generalised lymphadenopathy. In the present study an attempt is made to identify and categorise various neoplastic versus nonneoplastic lesions in patients presenting with generalised lymphadenopathy in a tertiary care centre.METHODS A cross sectional study of 100 lymph-node biopsies was conducted. After adequate fixation in 10 % formalin, tissues were routinely processed and stained with haematoxylin and eosin (H & E). Special stains like Ziehl-Neelsen and reticulin were employed whenever indicated. Immunohistochemistry (IHC) was performed for all neoplastic lesions and for some cases of non-neoplastic lesions. RESULTS Amongst 100 cases of lymph node biopsies analysed in this study, 71 cases were non-neoplastic and 29 were neoplastic. The most common non-neoplastic lesion observed was granulomatous lymphadenitis (32 cases), followed by reactive lymphadenitis (20). 25 cases of non-Hodgkin lymphoma (NHL) and 4 cases of Hodgkin lymphoma were observed in the neoplastic group. In the age range of 4.5 to 77 years included in the study, major bulk of cases was found in the age group of 11 - 20 years; out of this, 78.9 % were non neoplastic and 21.1 % were neoplastic. It was observed that 100 % of cases in the age group below 10 years were non-neoplastic, whereas in the age group above 70 years, all cases were neoplastic. CONCLUSIONS Granulomatous lymphadenitis was the most common lesion observed, probably because of high incidence of tuberculosis in this part of state. Morphological evaluation by light microscopy supplemented by special stains and IHC are necessary for establishing final diagnosis of generalised lymphadenopathy. KEYWORDS Generalised Lymphadenopathy, Haematoxylin and Eosin, IHC, Granulomatous Lymphadenitis, Kikuchi Fujimoto Lymphadenopathy, Hodgkin Lymphoma, NonHodgkin Lymphoma

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