scholarly journals Activity Of Enteral Lactase And Lactobacilli In Maternal And Hereditary Toxic Anemia

2021 ◽  
Vol 03 (06) ◽  
pp. 51-57
Author(s):  
Kudeshova G.T. ◽  
◽  
Kuchkarova L.S. ◽  
Keyword(s):  
Enzyme Activity ◽  
Small Intestine ◽  
Lactase Activity ◽  
Growing Rats ◽  
Phenylhydrazine Hydrochloride ◽  
White Rats ◽  
Milk Sugar ◽  
Mother And Child ◽  
Old Rats

In this paper, the activity of lactobacilli in the small intestine and lactobacilli in the colon, which are involved in the digestion of milk sugar during lactotrophy using phenylhydrazine hydrochloride, studied the effects of toxic anemia on mother and child. Erythrocyte counts, hemoglobin levels, lactase enzyme activity in the small intestine, and lactobacilli activity in the colon were determined in 12- and 24-day-old rats born to mother anemic rats with toxic anemia under the influence of phenylhydrazine hydrochloride, and in growing rats after mother and offspring toxic anemia. Experiments in white rats have shown that in rats growing in experimental toxic anemia of mother and offspring, there is an increase in lactase activity in the small intestine and a decrease in lactobacilli activity depending on the degree of intoxication in the colon.

Development and tissue distribution of sucrase-isomaltase mRNA in rats

1990 ◽  
Vol 258 (1) ◽  
pp. G52-G58 ◽  
Author(s):  
L. L. Leeper ◽  
S. J. Henning
Keyword(s):  
Enzyme Activity ◽  
Small Intestine ◽  
Northern Blot ◽  
Blot Analysis ◽  
Molecular Basis ◽  
Northern Blot Analysis ◽  
Sucrase Activity ◽  
Old Rats ◽  
Two Parameters ◽  
Precocious Development

Previous studies of sucrase-isomaltase (SI) activities have shown this complex to be absent in the suckling rat and to appear during the weaning period. We describe here the cloning of a heterologous SI cDNA and its use for the quantitation of SI mRNA as a first step toward understanding the molecular basis of SI development. A survey of RNA from 12 tissues of mature rats by Northern blot analysis showed a 6-kb band of SI mRNA only in the small intestine. Within the latter, both sucrase activity and SI mRNA peaked in the jejunum. Assay of jejunal tissue from developing rats showed sucrase activity and SI mRNA to be first detectable at 18 days, to rise in parallel through 24 days, and then to diverge a little (enzyme activity being lower) by 36 days. When glucocorticoid was administered to 10-day-old rats, neither sucrase activity nor SI mRNA was detectable 12 h later. Both parameters were readily detected 24 h postinjection, although the mRNA had risen relatively more than the enzyme activity. The two parameters increased in concert through 5 days postinjection and then plateaued. We conclude that, with respect to distribution along the intestine and to normal and precocious development, activities of SI in the rat are determined primarily by the abundance of its mRNA.

scholarly journals Regulation of trypsin-like enzyme activity in the tissues of white rats with thiamine

2016 ◽  
Vol 71 (1) ◽  
pp. 77-81
Author(s):  
O. Ustjansky ◽  
S. Petrov ◽  
O. Budnyak ◽  
S. Gozhenk
Keyword(s):  
Enzyme Activity ◽  
Small Intestine ◽  
Protease Activity ◽  
Maximum Activity ◽  
Parenteral Administration ◽  
White Rats ◽  
Intact Rats

The effect of intramuscular thiamine injection on the activity of trypsin-like enzymes in the liver, kidneys, stomach and small intestine of white rats has been researched. In the organs of intact rats, the maximum activity of trypsin-like proteases has been established in the small intestine, and the minimum one in the liver. Thiamine parenteral administration leads to the decrease of trypsin-like protease activity in the liver, kidney and stomach, and the increase of the enzyme activity in the small intestine that suggests the possibility of non-coenzyme thiamine effect on the trypsin-like enzyme activity.

scholarly journals Phosphatidylcholine synthesis in the developing small intestine

1980 ◽  
Vol 186 (2) ◽  
pp. 399-403 ◽  
Author(s):  
P G Holtzapple ◽  
C M Starr ◽  
T Morck
Keyword(s):  
Small Intestine ◽  
Oleic Acid ◽  
Steady State ◽  
Adult Rats ◽  
Acyltransferase Activity ◽  
Acid Incorporation ◽  
Old Rats ◽  
Phosphatidylcholine Synthesis

1. Phosphatidylcholine synthesis in the foetal, newborn and adult small intestine of rats was studied by determination of cytidine diphosphocholine-1,2-diacylglycerocholine phosphotransferase (cholinephosphotransferase) and acyl-CoA-1-acyl-sn-glycerol-3-phosphocholine acyltransferase (lysophosphatidylcholine acyltransferase) activities and the incorporation of [1-14C]oleic acid into phosphatidylcholine. 2. Cholinephosphotransferase activity was low in foetal jejunum and ileum, increased 3-4 fold in the ileum by 6 days of age and by 12 days in the jejunum. Jejunal activity remained constant throughout weaning; ileal activity gradually decreased to values 25% of that of the jejunum. 3. Lysophosphatidylcholine acyltransferase activity was high in foetal jejunum and ileum, decreased 70% immediately after birth in the jejunum and increased to adult values by 12 days of age. Ileal activity decreased by 20% after birth, but decreased more rapidly at weaning to 30% of the activity in jejunum. 4. Initial rates and steady-state incorporation of [1-14C]oleic acid into phosphatidylcholine by jejunal rings of 10 day-old rats exceeded that observed in jejunal rings from adult rats by 2-4-fold. 5. In the postnatal jejunum, neither cholinephosphotransferase and lysophosphatidylcholine acyltransferase activities nor oleic acid incorporation were stimulated by cortisone administration in vivo.

Peculiarities of the ultrastructure of neurons of the neocortex of 5-day-day rats under conditions of prenatal alcoholization

2021 ◽  
Vol 23 (1) ◽  
pp. 35-38
Author(s):  
L. I. Bon ◽  
◽  
S. M. Zimatkin ◽  
Keyword(s):  
Oxidative Stress ◽  
Pyramidal Neurons ◽  
Direct Action ◽  
Ultrastructural Changes ◽  
Initial Weight ◽  
Rat Pups ◽  
White Rats ◽  
Old Rats ◽  
Antenatal Alcoholization

The aim of this work was to study the ultrastructure of the internal pyramidal neurons of the neocortex of 5-day-old rat pups after antenatal alcoholization. The studies were carried out on female outbred white rats with an initial weight of 230 ± 20 g and their offspring. Prenatal alcoholization causes deep and varied ultrastructural changes in pyramidal neurons in the neocortex of 5-day-old rats. Moreover, these violations of direct action not only as a consequence of the damaging effect of alcohol, its metabolite acetehyde or the oxidative stress they cause on the membranes and organelles of neurons during embryogenesis, but also as a violation of the normal "program" of development" of neurons in the cortex.

THE EFFECT OF FASTING ON DISACCHARIDASE ACTIVITY IN THE RAT SMALL INTESTINE

PEDIATRICS ◽  
1971 ◽  
Vol 47 (1) ◽  
pp. 65-72
Author(s):  
L. K. McNeill ◽  
J. R. Hamilton
Keyword(s):  
Small Intestine ◽  
Epithelial Cells ◽  
Specific Activity ◽  
Cell Mass ◽  
Free Access ◽  
Lactase Activity ◽  
Childhood Diseases ◽  
Mucosal Protein ◽  
And Function ◽  
Fasted Rats

We assessed intestinal structure, mucosal epithelial kinetics, and disaccharidase activities after fasting. Rats fasted for up to 120 hours were compared with control rats fed ad libitum. All rats had free access to water and all were prevented from ingesting their own stools. Body weight, small intestinal weight and mucosal protein, and maltase and sucrase activity of the total small intestine decreased in fasted rats. Lactase activity did not decrease. Specific activity of lactase actually increased in the jejunum. Assessed after a 96-hour fast, jejunal villi were shortened with less epithelial cells along their length and the rate of migration of those cells along the villi was diminished in the fasted rats compared with control rats. We attribute the decreased total intestinal sucrase and maltase activities to a loss of total epithelial cell mass in the small bowel. An abnormality in the cells of the progenitor zone of the crypts is suggested by the decreased migration rate of mucosal epithelial cells in fasting rats. These factors do not explain our observations completely since lactase activity did not diminish. We postulate that the activity of the "acid" β-galactosidase located in the cytoplasm or lysosomes of the epithelial cells was stimulated by fasting. Our observations are relevant to clinical pediatrics. Undernutrition and fasting my be associated with many childhood diseases and with treatment of disease. In assessing clinical data and advising treatment, the pediatrician should be aware of the potentially harmful effects of starvation on intestinal structure and function.

Relation between dietary-induced increase of intestinal lactase activity and lactose digestion and absorption in adult rats

1984 ◽  
Vol 247 (6) ◽  
pp. G729-G735
Author(s):  
J. Leichter ◽  
T. Goda ◽  
S. D. Bhandari ◽  
S. Bustamante ◽  
O. Koldovsky
Keyword(s):  
Glucose Absorption ◽  
Diet Group ◽  
Lactase Activity ◽  
Weight Changes ◽  
Adult Rats ◽  
High Starch ◽  
Old Rats ◽  
Starch Diet

To study the relation between dietary-induced increase of intestinal lactase activity and lactose absorption, 11-wk-old rats were fed either a high-starch (70 cal%), low-fat (7 cal%) diet or a low-starch (5 cal%), high-fat (73 cal%) diet for 7 days. Food intake and body weight changes were similar in the two dietary groups. In the first experiment, lactose absorption was studied in vivo after oral administration of 600 mg lactose (10% solution in water with added [3H]PEG) to rats fasted for 16 h. Groups of rats were killed at time 0 and at 1-h intervals for the next 3 h. Lactase activity and lactose absorption were significantly higher (P less than 0.01) in the high-starch group than in the low-starch group. In the subsequent experiment, 9-wk-old rats were fed the two isocaloric diets for 3 days. By use of the everted sac technique, we have demonstrated a significantly higher absorption of monosaccharides from lactose in the high-starch diet group; also, glucose transport was higher in the high-starch diet-fed animals. When Tris, an inhibitor of lactase, was added into the mucosal fluid, absorption of lactose was abolished and no effect was seen on glucose absorption (in vivo and in vitro). In both experiments, significant linear regression was established between lactase activity and lactose absorption. Our results thus show that the increase in lactase activity, induced by feeding a high-starch diet to adult rats, is accompanied by an increased capacity to hydrolyze lactose and absorb the constituent monosaccharides.

Ornithine decarboxylase in rat small intestine: stimulation with food or insulin

1976 ◽  
Vol 231 (5) ◽  
pp. 1557-1561 ◽  
Author(s):  
DV Maudsley ◽  
J Leif ◽  
Y Kobayashi
Keyword(s):  
Enzyme Activity ◽  
Small Intestine ◽  
Ornithine Decarboxylase ◽  
Diamine Oxidase ◽  
Actinomycin D ◽  
Histidine Decarboxylase ◽  
Decarboxylase Activity ◽  
Adenosylmethionine Decarboxylase ◽  
Diamine Oxidase Activity ◽  
Similarities And Differences

Ornithine decarboxylase in the small intestine of starved rats was stimulated 3- to 10-fold by refeeding or administration of insulin. A peak is observed 3-5 h following treatment after which the enzyme activity rapidly declines. The rise in ornithine decarboxylase is reduced by actinomycin D or cycloheximide. The increase in enzyme activity occurs mainly in the duodenum and jejunum with less than a twofold change being observed in the ileum. A small (twofold) increase in S-adenosylmethionine decarboxylase activity in the small intestine was observed after food, but there was no change in diamine oxidase activity. Whereas pentagastrin and metiamide administration markedly stimulated histidine decarbosylase in the gastric mucosa, no consistent effect of these agents on ornithine decarboxylase in the small intestine was observed. The similarities and differences between histidine decarboxylase and ornithine decarboxylase are discussed.

EXCRETION OF CATECHOL AMINES BY WHITE RATS IN THE COLD

1964 ◽  
Vol 42 (4) ◽  
pp. 567-577 ◽  
Author(s):  
Lorraine C. Smith ◽  
L.-P. Dugal
Keyword(s):  
The Other ◽  
Sprague Dawley ◽  
White Rats ◽  
Sprague Dawley Rats ◽  
Old Rats ◽  
Catechol Amines ◽  
Sustained Increase

Exposure of white rats to 2 °C for 20 weeks caused an immediate and sustained increase in the excretion of catechol amines as compared to controls kept at 23 °C. Adrenaline excretion increased approximately three to four times while noradrenaline excretion increased about eight times. There were no marked differences between Wistar and Sprague–Dawley rats in the amounts of adrenaline and noradrenaline excreted at 23 °C or 2 °C. 'Old' rats kept in activity cages excreted much more of the catechol amines both at 23 °C and 2 °C than did the other rats and they showed a peak for adrenaline excretion after 1 week and for noradrenaline excretion after 3 to 4 weeks in the cold.

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