scholarly journals DEVELOPMENT AND VALIDATION OF AN ANALYTICAL METHOD FOR CONDENSED TANNIN EXTRACTS OBTAINED FROM THE BARK OF FOUR TREE SPECIES USING HPLC

Wood Research ◽  
2021 ◽  
Vol 66 (2) ◽  
pp. 171-182
Author(s):  
Faustino Ruiz Aquino ◽  
Rossy Feria Reyes ◽  
José Guadalupe Rutiaga Quiñones ◽  
Wenceslao Santiago García ◽  
Mario Ernesto Suárez Mota ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Analytical Method ◽  
Tree Species ◽  
Condensed Tannins ◽  
High Performance ◽  
Condensed Tannin ◽  
Hydroxyl Group ◽  
Aromatic Esters ◽  
Development And Validation ◽  
Method Of Extraction

Herein, we evaluated the content of condensed tannins present in the bark of four tree species that are Quercus laurina Humb. & Bonpl., Quercus crassifolia Humb. & Bonpl., Arbutus xalapensis Kunth, and Prunus serotina Ehrn. An analytical method using high-performance liquid chromatography (HPLC) for condensed tannin extracts was developed and validated. Also, the aqueous extracts were analyzed by Fourier transform infrared spectroscopy (FTIR). Based on the Stiasny number, A. xalapensis, and Q. laurina represent an important source of condensed tannins, which may be subject to exploitation. Using infrared spectroscopy, it was observed that tannins do not show an important signal of carbonyl groups (aromatic esters) with respect to high purity catechin. Furthermore, the band of the hydroxyl group is less pronounced in tannins, because various hydroxyl are interacting with each other. However, it can be seen that the method of extraction of wood tannins developed in this work, presents satisfactory results.

Development and validation of a HPLC-UV method for the simultaneous detection and quantification of paclitaxel and sulforaphane in lipid based self-microemulsifying formulation

2019 ◽  
Vol 57 (10) ◽  
pp. 931-938 ◽  
Author(s):  
Mohammad M Kamal ◽  
Sami Nazzal
Keyword(s):  
Analytical Method ◽  
Mobile Phase ◽  
High Performance ◽  
Limit Of Detection ◽  
Simultaneous Detection ◽  
Reversed Phase ◽  
Limit Of Quantitation ◽  
Development And Validation ◽  
Simultaneous Delivery ◽  
Detection And Quantification

Abstract Paclitaxel (PTX) and sulforaphane (SFN) are known anticancer molecules. Their activity was found to be potentiated when tested concurrently. Only recently, however, a novel SFN enabled PTX self-microemulsifying formulation (SMEDDS) was developed for their simultaneous delivery. This necessitated the development of an analytical method for the simultaneous detection and quantitation of PTX and SFN. In this study, a simple and sensitive isocratic high performance liquid chromatography-ultraviolet (HPLC-UV) analytical method was developed and validated per International Conference on Harmonization guidelines to satisfy this objective. Its application was demonstrated when quantifying the amount of PTX and SFN released from the SMEDDS in various dissolution media. The separation of the analytes was performed with the aid of a reversed phase C18 column at ambient temperature using a 60:40 mixture of acetonitrile and KH2PO4 buffer (pH 5.0) as the mobile phase. PTX and SFN peaks were detected at 202 nm with high resolution without interference from excipients. This method showed linearity within 2.5–100 μg/mL range with r2 > 0.999. The limit of detection and lower limit of quantitation were 0.1638 and 0.4964 μg/mL for PTX and 0.4419 and 1.3389 μg/mL for SFN, respectively. A total of 98–101% of the injected samples was recovered with RSD of 0.06–0.68% indicating the suitability of the method for the simultaneous detection and quantitation of the molecules in dissolution media.

A REVIEW ON METHOD DEVELOPMENT AND VALIDATION BY USING HPLC

2021 ◽  
pp. 34-37
Author(s):  
Dipali S. Doifode ◽  
Shailesh G. Jawarkar ◽  
Monika P. Jadhao ◽  
Manali M. Bode
Keyword(s):  
Quality Assurance ◽  
Analytical Method ◽  
High Performance ◽  
Method Development ◽  
Limit Of Detection ◽  
Science Research ◽  
Hplc Method ◽  
Pharmaceutical Drugs ◽  
Method Development And Validation ◽  
Development And Validation

Analytical method development and validation are the continuous and inter-dependent task associated with the research and development, quality control and quality assurance departments. High performance liquid chromatography (HPLC) has been widely used for years as an analytical method and is a key tool for the separation and analysis of pharmaceutical drugs, for drug monitoring and for quality assurance and life science research. Most of the drugs in multi component dosage forms can be analysed by HPLC method because of the several advantages like rapidity, specicity, accuracy, precision and ease of automation in this method. HPLC methods development and validation play important roles in new discovery, development, manufacture of pharmaceutical drugs and various other studies related to humans and animals. This review gives information regarding principle, types, instrumentation and along with various application of the method and Validation of HPLC method as per ICH Guidelines covers all the performance characteristics of validation, like Accuracy, precision, specicity, linearity, range and limit of detection, limit of quantication, robustness

Development and Validation of a Method for the Detection and Quantification of Antidepressants and Antipsychotics in Bone Samples by GC–MS

2020 ◽  
Author(s):  
Antonino Laudani ◽  
Deborah Sangregorio ◽  
Pietro Zuccarello ◽  
Teresa Licciardello ◽  
Stephanie Grasso ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Analytical Method ◽  
Forensic Toxicology ◽  
Selected Ion Monitoring ◽  
Method Performance ◽  
Matrix Interferences ◽  
Potential Alternative ◽  
Development And Validation ◽  
Method Of Extraction ◽  
Human Autopsy

Abstract Bone marrow is the tissue contained inside the bones and can be considered one of the potential alternative tissues in forensic toxicology. This matrix could be particularly useful in those cases where the routine sample is not available due to an advanced state of decomposition or skeletonization of the corpse. The aim of this study was to develop, validate and apply an analytical method of extraction and analysis of different antidepressants and antipsychotics, commonly used in therapy, from spiked pig ribs. Pig ribs, each of 5 g and 5 cm long, were spiked at 3 concentration levels (100, 200 and 500 ng/g). For each concentration, 10 pig ribs were prepared. The method involves the leaching by ethanol at different pHs of nine drugs from the inside of ribs, in particular from the bone marrow, without the fragmentation of bone tissue. Following a liquid–liquid purification and extraction, analysis was performed by gas chromatography coupled with mass spectrometry in selected ion monitoring mode. For method validation was assessed linearity, sensitivity, precision and accuracy, matrix interferences and, finally, carryover. Analytical method performance was acceptable respect to acceptance criteria for validation. No matrix interferences were detected; for this reason, it is possible to affirm that this method has a good selectivity. Moreover, the method was not affected by carryover. Considering that the study conducted on pig ribs has given encouraging results, it can be assumed that this method can be used in forensic toxicological protocols (human autopsy cases) as alternative to classic procedures.

scholarly journals Development and Validation of a Rapid Analytical Method for the Simultaneous Quantification of Metabolic Syndrome Drugs by HPLC-DAD Chromatography

2021 ◽  
Vol 89 (1) ◽  
pp. 8
Author(s):  
Jorge Cruz-Angeles ◽  
Luz María Martínez ◽  
Marcelo Videa ◽  
José Rodríguez-Rodríguez ◽  
Cecilia Martínez-Jiménez
Keyword(s):  
Metabolic Syndrome ◽  
Analytical Method ◽  
High Performance ◽  
Pharmaceutical Products ◽  
Array Detector ◽  
Simultaneous Treatment ◽  
Simultaneous Quantification ◽  
The Metabolic Syndrome ◽  
Pharmaceutical Form ◽  
Development And Validation

Worldwide, 25% of the population suffers from metabolic syndrome (MetS). The treatment of patients with MetS regularly includes drugs prescribed simultaneously to treat several disorders that manifest at the same time, such as hypercholesterolemia, arterial hypertension, and diabetes. To the authors’ best knowledge, there is no previous published analytical method for the simultaneous quantification of drugs used in the treatment of these diseases. In the present study, a rapid high-performance liquid chromatography with a diode-array detector HPLC-DAD methodology was developed for simultaneous quantification of carvedilol (CVD), telmisartan (TEL), bezafibrate (BZT), gliclazide (GZD), and glimepiride (GMP) in bulk and pharmaceutical form. The chromatographic separation of the five pharmaceuticals was achieved on a Hypersil GOLD C18 Selectivity (5 µm, 150 × 4.60 mm2) using a mobile phase of acetonitrile (50%) and 0.02 M KH2PO4, pH 3 (50%) at a flow rate of 1 mL/min and at 25 °C. The total separation time was 9 min. The analytical method was validated following the International Conference on Harmonization guidelines. A reproducible method was obtained with acceptable limits of detection (LOD) and quantification (LOQ) for CVD (0.012 and 0.035 μg mL−1), TEL (0.103 and 0.313 μg mL−1), BZT (0.025 and 0.076 μg mL−1), GZD (0.039 and 0.117 μg mL−1), and GMP (0.064 and 0.127 μg mL−1). The validated method allowed the determination of these drugs in commercial pharmaceutical products both individually and simultaneously. The present method was found to be suitable for simultaneous quantification of the five drugs that are most commonly used in the simultaneous treatment of the metabolic syndrome.

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