scholarly journals Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells

10.3791/1656 ◽  
2009 ◽  
Author(s):  
William N. White ◽  
Palaniappan Sethu
Keyword(s):  
Blood Lysis

scholarly journals Validation of SYBR green I based closed tube loop mediated isothermal amplification (LAMP) assay and simplified direct-blood-lysis (DBL)-LAMP assay for diagnosis of visceral leishmaniasis (VL)

2018 ◽  
Vol 12 (11) ◽  
pp. e0006922 ◽  
Author(s):  
Keerti Kaumudee Dixit ◽  
Sandeep Verma ◽  
Om Prakash Singh ◽  
Dharmendra Singh ◽  
Akhil Pratap Singh ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Lamp Assay ◽  
Sybr Green ◽  
Isothermal Amplification ◽  
Sybr Green I ◽  
Loop Mediated Isothermal Amplification ◽  
Closed Tube ◽  
Blood Lysis

scholarly journals The relationship of the dilute whole blood lysis time to the fibrinolytic activity of blood: effect of change in plasma fibrinogen

1973 ◽  
Vol 26 (3) ◽  
pp. 189-193 ◽  
Author(s):  
J. A. Hickman ◽  
I. C. Gordon-Smith ◽  
P. F. Whitfield ◽  
S. J. Godfrey
Keyword(s):  
Whole Blood ◽  
Fibrinolytic Activity ◽  
Plasma Fibrinogen ◽  
Lysis Time ◽  
Blood Lysis ◽  
Relationship Of ◽  
The Relationship

scholarly journals Comparative analysis of whole blood lysis methods for flow cytometry

Cytometry ◽  
1997 ◽  
Vol 30 (3) ◽  
pp. 124-133 ◽  
Author(s):  
Xavier Bossuyt ◽  
Gerald E. Marti ◽  
Thomas A. Fleisher
Keyword(s):  
Flow Cytometry ◽  
Comparative Analysis ◽  
Whole Blood ◽  
Blood Lysis

scholarly journals Effects of whole blood lysis and fixation on the infectivity of human T-lymphotropic virus type 1 (HTLV-I)

Cytometry ◽  
1994 ◽  
Vol 18 (1) ◽  
pp. 49-54 ◽  
Author(s):  
Julie G. Ericson ◽  
Alex V. Trevino ◽  
Gary P. Toedter ◽  
Lawrence E. Mathes ◽  
Garret C. Newbound ◽  
...  
Keyword(s):  
Virus Type ◽  
Whole Blood ◽  
T Lymphotropic Virus ◽  
Blood Lysis

scholarly journals Development of a Method to Detect Mycobacterium paratuberculosis in the Blood of Farmed Deer Using Actiphage® Rapid

2021 ◽  
Vol 8 ◽  
Author(s):  
Anton Kubala ◽  
Tania M. Perehinec ◽  
Catherine Evans ◽  
Andrea Pirovano ◽  
Benjamin M. C. Swift ◽  
...  
Keyword(s):  
Gradient Centrifugation ◽  
Elisa Test ◽  
Buffy Coat ◽  
Test Results ◽  
Blood Samples ◽  
Diagnostic Potential ◽  
Ficoll Gradient ◽  
Blood Lysis ◽  
Small Set ◽  
Cattle Blood

Mycobacterium avium subsp paratuberculosis (MAP) is the causative agent of Johne's disease, which is an economically and clinically relevant pathogen for commercial deer production. The purpose of this study was to develop a method that could be used to rapidly detect MAP infection in deer using the Actiphage Rapid blood test. This test has previously been used to detect MAP in cattle blood following the purification of buffy coat using Ficoll gradients, however this method is quite laborious and costly. The purpose of this study was to develop a simpler method of blood preparation that was also compatible with deer blood and the Actiphage test. Initially differential lysis of RBCs using Ammonium Chloride-Potassium (ACK) blood lysis buffer was compared with the Ficoll gradient centrifugation method using cattle blood samples for compatibility with the Actiphage reagents, and it was found that the simpler ACK method did not have an impact on the Actiphage test reagents, producing an equivalent sensitivity for detection of low levels of MAP. When the two methods were compared using clinical blood samples from farmed deer, the ACK lysis method resulted in a cleaner sample. When a blinded test of 132 animals from 4 different production groups was carried out, the majority of the positive test results were found to be from animals in just one group, with a small number identified in a second group. The test results were found to be reproducible when a small set of positive animals were tested again 1 month after their initial testing. Finally a set of negative animals which had been previously screened using an ELISA test, all animals gave a negative Actiphage result. This study shows that this improved sample preparation method and Actiphage blood testing can be used to test blood samples from deer, and the full diagnostic potential of the method can now be evaluated.

Insulin and Blood Fibrinolytic Activity

1973 ◽  
Vol 29 (02) ◽  
pp. 293-299 ◽  
Author(s):  
Anne M Hedlin
Keyword(s):  
Glucose Tolerance ◽  
Fibrinolytic Activity ◽  
Glucose Tolerance Test ◽  
Tolerance Test ◽  
Control Subjects ◽  
Insulin Tolerance ◽  
Hospital Patients ◽  
Blood Lysis ◽  
And Control ◽  
Increased Activity

SummaryThe effect of insulin on fibrinolysis was studied in hospital patients who were subjected to an insulin tolerance or glucose tolerance test. Blood samples were obtained at frequent intervals over a 2-3 hour period and fibrinolytic activity determined by the dilute whole blood lysis test. Normal control subjects were tested in both fasting and in non-fasting states. A marked increase in fibrinolytic activity was produced during the insulin tolerance test. However, as this increase did not appear until the blood glucose had been reduced to 30-40 mg % it was considered to be due, not to the insulin which had been administered, but rather to the action of those hormones which would be released in response to the hypoglycemia. On the other hand, the results of the glucose tolerance test demonstrated a gradual increase in fibrinolysis during the test period. Again this increased activity could not be attributed to the action of insulin because a similar effect was apparent in the samples obtained from the control subjects. Therefore, it was concluded that the enhancement of fibrinolysis observed in both test and control subjects was not due to the action of insulin.

scholarly journals Rapid automated disgnosis of bacteremia by impedance detection

1977 ◽  
Vol 5 (1) ◽  
pp. 51-57
Author(s):  
R L Kagan ◽  
W H Schuette ◽  
C H Zierdt ◽  
J D MacLowry
Keyword(s):  
Blood Culture ◽  
Detection Efficiency ◽  
Detection System ◽  
Blood Cultures ◽  
Automated Detection ◽  
Culture Bottle ◽  
System A ◽  
Blood Lysis ◽  
Filtration Technique ◽  
New System

Lysis and filtration of blood culture specimens were combined with impedance detection of bacterial growth to facilitate the diagnosis of bacteremia. A blood lysis-filtration technique (Zierdt et al., 1976) was coupled to a simple, inexpensive automated detection system. The practical and technical aspects of the impedance detection system are discussed. This new blood culturing system was compared to a conventional system for 264 aerobic blood cultures. A 30-ml sample of the blood-broth mixture was withdrawn from the conventional aerobic blood culture bottle and processed in parallel. Excluding the isolation of the commonly recognized contaminants, the detection efficiency was 36% greater in the new system. A total of 53 blood cultures from 107 patients were positive by one or both methods. The new system detected 92% of the total number of positive cultures, compared with 56% detected by the routine method. The explanation of the differences is discussed.

scholarly journals Flow cytometric analysis of whole blood lysis, three anticoagulants, and five cell preparations

Cytometry ◽  
1992 ◽  
Vol 13 (1) ◽  
pp. 68-74 ◽  
Author(s):  
Patricia H. Carter ◽  
Sandra Resto-Ruiz ◽  
Glennelle C. Washington ◽  
Steve Ethridge ◽  
Alessio Palini ◽  
...  
Keyword(s):  
Whole Blood ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric ◽  
Blood Lysis

Ficoll-hypaque separation vs whole blood lysis: Comparison of efficiency and impact on minimal residual disease analysis

2017 ◽  
Vol 40 (2) ◽  
pp. 201-208 ◽  
Author(s):  
E. Genuardi ◽  
D. Barbero ◽  
I. Dogliotti ◽  
B. Mantoan ◽  
D. Drandi ◽  
...  
Keyword(s):  
Minimal Residual Disease ◽  
Whole Blood ◽  
Residual Disease ◽  
Blood Lysis ◽  
Disease Analysis ◽  
Minimal Residual

Increased von Willebrand factor, P-selectin and fibrin content in occlusive thrombus resistant to lytic therapy

2016 ◽  
Vol 115 (06) ◽  
pp. 1129-1137 ◽  
Author(s):  
Bruno Del Blanco ◽  
Marisol Ruiz-Meana ◽  
Jaume Francisco ◽  
José Barrabés ◽  
Jaume Figueras ◽  
...  
Keyword(s):  
Coronary Intervention ◽  
Clot Lysis ◽  
St Segment Elevation ◽  
Coronary Thrombus ◽  
Healthy Donors ◽  
St Segment ◽  
Blood Lysis ◽  
Pai 1 ◽  
D Dimer

SummaryTherapeutic fibrinolysis is ineffective in 40 % of ST-segment elevation acute myocardial infarction (STEMI) patients, but understanding of the mechanisms is incomplete. It was our aim to compare the composition of coronary thrombus in lysis-resistant STEMI patients with that of lysis-sensitive patients. Intracoronary thrombi (n=64) were obtained by aspiration in consecutive STEMI patients. Of them, 20 had received fibrinolysis and underwent rescue percutaneous coronary intervention (r-PCI, lysis-resistant patients) and 44 underwent primary PCI (p-PCI). Lysis-sensitivity was determined in vitro by clot permeability measurements and turbidimetric lysis in plasma of 44 patients undergoing p-PCI and 20 healthy donors. Clot-lysis sensitivity was defined as a clot-lysis time not greater than 1 SD over the mean of healthy donors. Coronary thrombus composition in 20 lysis-resistant and in 20 lysissensitive patients was analysed by immunofluorescence with confocal microscopy. Plasma biomarkers (P-selectin, VWF, PAI-1, t-PA, D-dimer, TF pathway markers, plasmin and CD34+) were measured simultaneously on peripheral blood. Lysis-resistant clots had higher levels of fibrin (p=0.02), P-selectin (p=0.03) and VWF (p=0.01) than lysis-sensitive clots. Among thrombi obtained ≤ 6 hours after onset of symptoms, those from lysis-resistant patients showed a higher content in fibrin than those from p-PCI patients (p=0.01). Plasma PAI-1 (p=0.02) and D-dimer levels were significantly higher (p=0.003) in lysis-resistant patients, whereas plasmin levels were lower (p=0.03). Multivariate analysis showed the content of fibrin and VWF within thrombus as predictors of thrombolysis resistance. In conclusion, coronary thrombi in STEMI patients resistant to fibrinolysis are characterised by higher fibrin, P-selectin and VWF content than lysis-sensitive thrombi.

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