scholarly journals Anti-Epileptic Potential of Ziziphus Vulgaris and Ferula Asafoetida Extracts in Drug Induced Seizure Models of Experimental Mice

2020 ◽  
Vol 8 (1) ◽  
pp. 37-46
Author(s):  
Nadia Anwar ◽  
Mahtab Ahmad Khan ◽  
Mehwish Bukhari ◽  
Hammad Ahmed ◽  
Awais Ali Zaidi ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Seizure Frequency ◽  
Plant Extracts ◽  
Intraperitoneal Administration ◽  
Epileptic Activity ◽  
Positive Control ◽  
Histopathological Analysis ◽  
Significant Delay ◽  
Seizure Models ◽  
Ferula Asafoetida

Background: Ziziphus vulgaris (ZV) and Ferula asafoetida (FA) have phenolic compounds with potential anti-epileptic activity. Objective: This study was aimed to investigate the anti-epileptic potential of hydroalcoholic (30:70) crude extracts of ZV and FA. Methods: Different doses (5 mg/ml, 15 mg/ml, 25 mg/ml) of extracts from ZV and FA were separately administered intraperitoneally to groups (7/group) of male albino mice (20-30 g). Phenytoin (15 mg/ml, intraperitoneal) was used as positive control. After 30 min, tonic-clonic seizures were induced by intraperitoneal administration of picrotoxin (6 mg/ml) and strychnine (4 mg/ml) in separate groups. Animals were monitored for 1 h and different parameters including onset and frequency of seizures and protection (against mortality & seizures) were determined. Results: A dose dependent significant delay in onset and decrease in seizure frequency as well as mortality was observed in animals treated with plant extracts (ZV and FA). Positive control (phenytoin) also showed significant delay in seizure onset and decreased the seizure frequency. Conclusion: The plant extracts (ZV and FA) contain the phenolic compounds which may induce the GABAergic transmission that could be the most probable mechanism for their anti-epileptic activity. Molecular studies and histopathological analysis are required to elucidate the exact anti-epileptic mechanisms of ZV and FA extracts.

Capillary zone electrophoretic determination of phenolic compounds in chess (Bromus inermisL.) plant extracts

2006 ◽  
Vol 29 (2) ◽  
pp. 308-313 ◽  
Author(s):  
Dagmar Štěrbová ◽  
Jiří Vlček ◽  
Vlastimil Kubáň
Keyword(s):  
Phenolic Compounds ◽  
Plant Extracts ◽  
Capillary Zone

Antioxidant Activity of Plant Extracts Containing Phenolic Compounds

1999 ◽  
Vol 47 (10) ◽  
pp. 3954-3962 ◽  
Author(s):  
Marja P. Kähkönen ◽  
Anu I. Hopia ◽  
Heikki J. Vuorela ◽  
Jussi-Pekka Rauha ◽  
Kalevi Pihlaja ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Plant Extracts

scholarly journals Stimulation of phenolic compounds production in the in vitro cultivated Polyscias filicifolia Bailey shoots and evaluation of the antioxidant and cytotoxic potential of plant extracts

2018 ◽  
Vol 87 (2) ◽  
Author(s):  
Anita Agnieszka Śliwińska ◽  
Katarzyna Sykłowska-Baranek ◽  
Anita Kośmider ◽  
Sebastian Granica ◽  
Karolina Miszczak ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Cytotoxic Activity ◽  
Phenolic Compound ◽  
Plant Extracts ◽  
Detrimental Effect ◽  
A549 Cells ◽  
Ferulic Acids

<p>In this study, an efficient method to enhance phenolic compound production in the in vitro cultured shoots of <em>Polyscias filicifolia</em> was developed. The phenolic compound content in <em>P. filicifolia</em> has not yet been reported. Shoots were treated with methyl jasmonate (JM) or salicylic acid (SA) at doses of 50, 100, or 200 µM. HPLC-UV-VIS and LC-MS techniques were used for the determination of chlorogenic, caffeic, and ferulic acids. The total phenolics and flavonoids were quantified, and the antioxidant capacity of plant extracts was determined using DPPH and ABTS methods. Finally, the cytotoxic activity of <em>P. filicifolia</em> extracts in normal (HaCaT) and cancer (A549) cells was investigated. Further, the effect of the extracts on cisplatin cytotoxicity was assessed.</p><p>The elicitors significantly enhanced phenolic production compared to that in untreated shoots and leaves of intact plants. Chlorogenic acid was the most abundant compound with the highest yield of 5.03 ±0.25 mg/g DW after treatment with 50 µM SA. The total flavonoid and phenolic content was significantly and dose-dependently influenced by JM. The highest antioxidant capacity was noted in extracts derived from shoots grown on media supplemented with 50 µM SA and 200 µM JM; these doses were used for further cytotoxic activity investigations. The extracts from JM or SA treatments reduced cancer cell viability and increased their mortality, whereas the extract from JM treatment exhibited protective effect on normal cells. Moreover, the comparison of cytotoxic properties of plant extracts and cisplatin indicated that plant phenolic compounds in combination with anticancer drugs could reduce the detrimental effect of the latter on human cells.</p>

scholarly journals Nitric oxide inhibitory and anti- Bacillus activity of phenolic compounds and plant extracts from Mesua species

2018 ◽  
Vol 28 (2) ◽  
pp. 231-234 ◽  
Author(s):  
Thiruventhan Karunakaran ◽  
Intan Safinar Ismail ◽  
Gwendoline Cheng Lian Ee ◽  
Siti Mariam Mohd Nor ◽  
Kalaivani Palachandran ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Phenolic Compounds ◽  
Plant Extracts

Analgesic efficacy of intraperitoneal administration of bupivacaine in cats

2016 ◽  
Vol 18 (11) ◽  
pp. 906-912 ◽  
Author(s):  
Javier Benito ◽  
Beatriz Monteiro ◽  
Anne-Marie Lavoie ◽  
Guy Beauchamp ◽  
B Duncan X Lascelles ◽  
...  
Keyword(s):  
Repeated Measures ◽  
Linear Models ◽  
Intraperitoneal Administration ◽  
Analgesic Efficacy ◽  
Pain Scale ◽  
Positive Control ◽  
Rescue Analgesia ◽  
Pain Scores ◽  
Blinded Study ◽  
Effect Of Treatment

Objectives The aim of this study was to evaluate the analgesic efficacy of intraperitoneal (IP) bupivacaine in cats undergoing ovariohysterectomy (OVH). Methods Forty-five cats were included in a randomized, prospective, blinded study after owners’ written consent was obtained. The anesthetic protocol included acepromazine–buprenorphine–propofol–isoflurane. A ventral midline incision was made and cats (n = 15/group) were administered either IP saline 0.9% (negative and positive control groups; NG and PG, respectively) or IP bupivacaine (2 mg/kg; bupivacaine group; BG). Cats in the PG received meloxicam (0.2 mg/kg SC). An OVH was performed and postoperative pain was evaluated using a dynamic interactive visual analog scale (DIVAS), the UNESP-Botucatu multidimensional composite pain scale (MCPS) and mechanical nociceptive thresholds (MNT) for up to 8 h after the end of surgery. Postoperative sedation was evaluated using DIVAS. Rescue analgesia was provided with buprenorphine and/or meloxicam. Repeated measures linear models and a Cochran–Mantel–Haenszel test were used for statistical analysis ( P <0.05). Results There was a significant effect of treatment on the number of times rescue analgesia was administered ( P = 0.002) (PG, n = 2, 13%; NG, n = 12, 80%; BG, n = 4, 27%) with the number of rescues being higher in the NG group than in the PG ( P = 0.0004) and BG ( P = 0.02) groups. The DIVAS, MCPS and MNT were significantly different when compared with baseline values at different time points; however, data were not significantly different among groups. Conclusions and relevance Treatments PG and BG produced similar analgesia in terms of pain scores, number of times rescue analgesia was administered and MNT. Based on rescue analgesia, IP administration of bupivacaine provides analgesia in cats after OVH.

scholarly journals Cassia tora Linn Cream Inhibits Ultraviolet-B-Induced Psoriasis in Rats

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Manmohan Singhal ◽  
Niraj Kansara
Keyword(s):  
Skin Diseases ◽  
Methanolic Extract ◽  
Positive Control ◽  
Ultraviolet B ◽  
Histopathological Analysis ◽  
Standard Group ◽  
Dermal Toxicity ◽  
Epidermal Thickness ◽  
Cassia Tora ◽  
Capillary Loop

The aim of present study was to determine the antipsoriatic activity of newly formulated O/W creams of methanolic extract of Cassia tora L. leaves by using ultraviolet-B-induced psoriasis in rat. The plant Cassia tora L. is traditionally claimed to be useful in the treatment of a number of skin diseases. However, there are no established scientific reports for its antipsoriatic activity. Methanolic Cassia tora L. leaves extract was used to prepare various concentrations of O/W creams and tested for acute dermal toxicity study. The different O/W creams showed good physical characteristics and passed the sensitivity, irritation, grittiness and bleeding test. The results of acute dermal toxicity showed that the creams were safe up to the dose of 2000 mg/kg. In case of psoriasis model, histopathological analysis revealed that there were absence of Munro's microabscess, elongation of rete ridges, and capillary loop dilation in the section in Test 2 (0.1%) and standard group. O/W creams and methanolic extract of Cassia tora L. leaves exhibited significant reduction in percentage of relative epidermal thickness and spleen index as compared to positive control. We concluded that topical O/W creams and crude extract containing methanolic extract of Cassia tora L. leaves have potent antipsoriatic activity in ultraviolet-B-induced psoriasis in rat.

scholarly journals Evaluation of Mesenchymal Cells (MSC) and Dapsone for the Treatment of Dermonecrotic Wounds Caused by Loxosceles laeta Venom in Rabbits

2018 ◽  
Author(s):  
Guilherme Martins ◽  
Maira Oliveira ◽  
Ana Flávia Botelho ◽  
Conrado Gamba ◽  
Clara Duarte ◽  
...  
Keyword(s):  
Stem Cells ◽  
Positive Control ◽  
Negative Control ◽  
Intradermal Injection ◽  
Wound Contraction ◽  
Histopathological Analysis ◽  
Male Adult ◽  
Regenerative Therapies ◽  
Phosphate Buffered Saline ◽  
Loxosceles Laeta

We studied the efficacy of mesenchymal stem cells (MSC), either alone or associated with dapsone (DAP) in the treatment of dermonecrotic wounds caused by Loxosceles laeta spider venom. Twenty-five male adult rabbits were distributed into five groups, of which four groups received an intradermal injection of 20 &mu;g of L. laeta venom and only one received ultrapure water (negative control). After 4 hours, each group that received venom, was treated with MSC, DAP, MSC + DAP and Phosphate-buffered saline &ndash; PBS (positive control). Photographic records were made for analysis of the wound area evolution by morphometry. Twelve days after treatment, the skin samples around the lesion were removed for subsequent histological analysis. Concerning the rate of wound contraction, we observed that DAP showed the best percentage of contraction at day 3. In the treatments using MSCs, a negative value of wound contraction was observed for the isolated MSCs, as well as a lower contraction value for the association of the MSC + DAP when compared to PBS group. Histopathological analysis showed diminished tissue lesion and less intense inflammation in MSCs and DAP groups. This could indicated potential use of stem cells in regenerative therapies after loxoscelic accidents.

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