Chemical composition, antibacterial and antioxidant activities of Juniperus oxycedrus essential oils from North Western Algeria

The aim of the current study was to identify the essential oils of Juniperus oxycedrus from North Western Algeria (Mostaganem) by gas chromatography coupled to mass spectrometer (GC-MS), and to evaluate their antibac-terial and antioxidant activities. Extraction was carried out by two processes, hydrodistillation (HD) and steam distillation (SD). Analysis by GC-MS led to the identification of 38 components using both methods. HD was found to be the best process for the extraction of Juniperus essential oil, and the ma-jor identified compounds were: germacrene-D (38.28%), followed by α.-pinene (17.66%), and δ-cadinene (5.91%). In the antibacterial test, SD oil had a higher inhibitory effect. The strain Staphylococcus aureus was the most sensitive with an inhibition zone of 15.25 mm and a minimum inhibitory concentration (MIC) of 0.25 mg/mL. The antioxidant activity of J. oxycedrus es-sential oils obtained by HD was more important than SD (IC50= 19.52 mg/mL and IC50= 38.62 mg/mL) with DPPH and β-carotene bleaching tests respec-tively. Essential oils of J. oxycedrus could therefore represent good candidates to be used as antioxidant and antibacterial agents.

Download Full-text

Chemical Composition, Antibacterial and Antioxidant Activities of the Essential Oils from Thymus satureioides and Thymus pallidus

Natural Product Communications ◽

10.1177/1934578x1100601025 ◽

2011 ◽

Vol 6 (10) ◽

pp. 1934578X1100601 ◽

Cited By ~ 3

Author(s):

Ghalbane Ichrak ◽

Belaqziz Rim ◽

Ait Said Loubna ◽

Oufdou Khalid ◽

Romane Abderrahmane ◽

...

Keyword(s):

Gas Chromatography ◽

Essential Oils ◽

Steam Distillation ◽

Antioxidant Activities ◽

Inhibition Zone ◽

Volatile Fraction ◽

Dpph Assay ◽

Thymus Satureioides ◽

Broth Dilution

This study was designed to examine the in vitro antibacterial and antioxidant activities of the essential oils (EOs) of Thymus satureioides ( T.s) and T. pallidus ( T.p). EOs were isolated by steam distillation and analyzed by capillary gas chromatography and gas chromatography coupled to mass spectrometry (GC–MS). The major constituents of the volatile fraction of T. satureioides were borneol (29.5%), carvacrol (9.1%), and β-caryophyllene (8.2%), while those of T. pallidus were camphor (29.8%), dihydrocarvone (17.6%), borneol (7.6%) and camphene (7.5%). The essential oils were tested against a panel of Gram+ and Gram- bacteria by using agar diffusion and broth dilution methods. The data indicated that the Gram-positive Bacillus subtilis was the most sensitive strain producing an average inhibition zone of 51.7 mm. Furthermore, Pseudomonas aeruginosa, known as a resistant strain, was also sensitive. The samples were also subjected to screening for their possible antioxidant activity by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The IC50 values of the oil of T. satureioides and T. pallidus were 0.32 and 11.6 mg/mL, respectively.

Download Full-text

Antifungal activity of Mentha piperita and Carum carvi essential oils

Zbornik Matice srpske za prirodne nauke ◽

10.2298/zmspn1733201p ◽

2017 ◽

pp. 201-207 ◽

Cited By ~ 1

Author(s):

Dragana Plavsic ◽

Gordana Dimic ◽

Djordje Psodorov ◽

Dragan Psodorov ◽

Ljubisa Saric ◽

...

Keyword(s):

Antifungal Activity ◽

Essential Oils ◽

Inhibition Zone ◽

Inhibitory Effect ◽

Biologically Active ◽

Mentha Piperita ◽

Diffusion Method ◽

Aromatic Plants ◽

Disc Diffusion Method ◽

Penicillium Aurantiogriseum

Aromatic plants are one of the most important sources of biologically active secondary metabolites, which possess various antimicrobial characteristics. The aim of this work was to examine the effect of antifungal activities of mint and caraway essential oils against the selected fungi. Eight species of molds were selected for antifungal testing: Alternaria alternata, Aspegillus flavus, A. niger, A. versicolor, Eurotium herbariorum, Penicillium aurantiogriseum, P. chrysogenum and P. expansum. Testing of essential oils antifungal activity against the selected species was conducted using the disc diffusion method by adding mint and caraway essential oils (0.5, 1, 5, and 10 ?l per disc). Antifungal activity of essential oils was expressed by the diameter of inhibition zone (mm). The most powerful effect of mint essential oil was recorded against E. herbariorum, as its growth was completely inhibited by the quantity of 5 ?l. The weakest inhibitory effect was observed against P. chrysogenum (inhibition zone 13.67 mm) by the quantity of 10 ?l. The most powerful antifungal activity of caraway was observed against E. herbariorum as growth was completely inhibited by the quantity of 10 ?l. The weakest inhibitory effect was observed against A. niger (inhibition zone 28 mm) by the quantity of 10 ?l.

Download Full-text

Assessment of in vitro Antioxidant and Antidiabetic Capacities of Medlar (Mespilus germanica)

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha47111325 ◽

2018 ◽

Vol 47 (2) ◽

pp. 384-389

Author(s):

Sebnem Selen ISBILIR ◽

Sevilay Inal KABALA ◽

Hulya YAGAR

Keyword(s):

Antioxidant Activity ◽

Leaf Extract ◽

Antioxidant Activities ◽

Inhibitory Effect ◽

Total Phenolic ◽

Flower Bud ◽

Ethanol Extracts ◽

Dpph Scavenging ◽

Β Carotene

The objective of the current study was to evaluate the antioxidant activity and enzyme inhibitory effect of different parts of medlar including fruit, leaf and flower bud by using various in vitro methods, and also determination of total phenolic and flavonoid content in the samples. Ethanol extracts of medlar parts were prepared and their antioxidant activities were determined using 1,1-diphenyl-2-picryl-hydrazil (DPPH•) scavenging and β-carotene bleaching methods. The leaf extract showed the strongest antioxidant activity. DPPHradical scavenging activity was in the order of BHA > leaf > bud > fruit. This ordering was the same for β-carotene bleaching activity, tocopherol > leaf > bud > fruit. The highest total phenolic (60.3 ± 1.69 mg GAE g-1 extract) and flavonoid (14.77 ± 1.15 mg QE g-1 extract) content were determined in leaf extract. For possible antidiabetic effects of extracts, α-amylase and α-glucosidase inhibitory activities were investigated, the bud extract showed the highest inhibition activities among the all extracts.

Download Full-text

(R)-(–)-Linalyl Acetate and (S)-(–)-Germacrene D from the Leaves of Mexican Bursera linanoe

Natural Product Communications ◽

10.1177/1934578x1000500301 ◽

2010 ◽

Vol 5 (3) ◽

pp. 1934578X1000500 ◽

Cited By ~ 1

Author(s):

Koji Noge ◽

Nobuhiro Shimizu ◽

Judith X. Becerra

Keyword(s):

Solvent Extraction ◽

Essential Oils ◽

Decomposition Product ◽

Steam Distillation ◽

Common Method ◽

Volatile Components ◽

Linalyl Acetate ◽

Germacrene D

The leaf volatile components of Mexican Bursera linanoe were identified as ( R)-(–)-linalyl acetate (57.6%; 95.5% ee) and ( S)-(–)-germacrene D (39.3%; 100% ee) by solvent extraction and GC–MS and chiral GC analyses. Linalool was previously reported as the major component from the leaves of B. linanoe. However, we believe that this is a decomposition product of linalyl acetate during steam distillation, a common method for extraction of essential oils. The chemically unique blend in the leaves of B. linanoe may act as a chemical barrier against its potential herbivores, Blepharida beetles that have a tendency for attacking chemically similar plants as hosts.

Download Full-text

Chemical compositon, antibacterial and antioxidant activities of Cnidium silaifolium ssp. orientale (Boiss.) Tutin essential oils

Grasas y Aceites ◽

10.3989/gya.1146192 ◽

2021 ◽

Vol 72 (2) ◽

pp. e403

Author(s):

A.E. Karadağ ◽

B. Demirci ◽

Ö. Çeçen ◽

F. Tosun ◽

F. Demirci

Keyword(s):

Essential Oils ◽

Antioxidant Activities ◽

Chemical Compositions ◽

Significant Activity ◽

Gram Positive Bacteria ◽

Microdilution Method ◽

Aerial Parts ◽

Germacrene D ◽

Different Parts

The chemical compositions of the essential oils (EOs) obtained by hydrodistillation from different parts of Cnidium silaifolium ssp. orientale (Boiss.) Tutin were analyzed both by GC-FID and GC/MS, simultaneously. One hundred nine compounds representing 90.1% of the total volatiles in the EOs were identified with the main characteristic compounds α-pinene (50.3%) in the root, germacrene D (20.3%) in the fruit, and β-caryophyllene (18.7%) in the aerial parts of C. silaifolium ssp. orientale. The antimicrobial activity against human pathogenic Gram-negative and Gram-positive bacteria was evaluated by the in vitro microdilution method. Antibacterial succeptibility was observed from the root and aerial part EOs against Staphylococcus aureus (0.039 and 0.156 mg/mL, respectively); while the fruit EO was most effective against Bacillus cereus at 0.07 mg/mL. The antioxidant capacities of the EOs were also evaluated by in vitro DPPH• and ABTS•+ scavenging assays, where no significant activity was observed compared to ascorbic acid and Trolox.

Download Full-text

Chemical Variability of the Essential Oil Isolated from Aerial Parts of Tetraclinis Articulata from North-Western Algeria

Natural Product Communications ◽

10.1177/1934578x1501000834 ◽

2015 ◽

Vol 10 (8) ◽

pp. 1934578X1501000 ◽

Cited By ~ 1

Author(s):

Maghnia Boussaïd ◽

Chahrazed Bekhechi ◽

Fawzia Beddou ◽

Daoudi Chabane Sari ◽

Ange Bighelli ◽

...

Keyword(s):

Essential Oil ◽

Bornyl Acetate ◽

Pca Analysis ◽

Aerial Parts ◽

Group I ◽

Tetraclinis Articulata ◽

Chemical Variability ◽

Germacrene D ◽

Western Algeria ◽

North Western

The objective was to investigate the yield and chemical composition of 50 essential oil samples isolated from leaves and flowers of Tetraclinis articulata harvested in eight locations (coastal township and highlands) of Tlemcen Province (North-Western Algeria). Essential oil yields varied drastically from sample to sample (0.03 to 0.86%, w/w). No direct correlation was observed between the yield and the altitude of the harvest areas. The oils consisted mainly of monoterpenes: α-pinene (9.2–56.5%), bornyl acetate (1.2–45.1%), camphor (0.5–40.3%), borneol (0.2–12.9%), limonene (3.6–12.5%), and myrcene (1.6–9.7%). Sesquiterpenes were represented by germacrene D (up to 14.2%) and (E)-β-caryophyllene (up to 13.3%). PCA analysis of the data allowed the distinction of two groups within the samples. The composition of group I (9 samples) was dominated by camphor, (Mean = 30.9%) followed by α-pinene (M = 19.1%) and bornyl acetate (M = 11.4%). Group II was divided into two sub-groups. Samples of sub-group IIA (8 samples) contained mainly α-pinene (M = 45.4%). Samples of the largest group IIB (33 samples) were characterized by similar contents of α-pinene (M = 28.2%) and bornyl acetate (M = 24.5%) and the occurrence of camphor to a lesser extent (M = 10.0%).

Download Full-text

The chemical composition, antimicrobial, and antioxidant activities of Pycnocycla spinosa and Pycnocyla flabellifolia essential oils

Zeitschrift für Naturforschung C ◽

10.1515/znc-2016-0038 ◽

2016 ◽

Vol 71 (11-12) ◽

pp. 403-408 ◽

Cited By ~ 2

Author(s):

Mohaddese Mahboubi ◽

Elaheh Mahdizadeh ◽

Rezvan Heidary Tabar

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Antioxidant Activities ◽

Inhibition Zone ◽

Chemical Compositions ◽

Caryophyllene Oxide ◽

Aerial Parts ◽

Dilution Assay ◽

Main Components ◽

Broth Dilution

Abstract The purpose of our study was to compare the chemical compositions and antimicrobial and antioxidant activities of Pycnocycla spinosa and Pycnocycla flabellifolia essential oils. cis-Asarone (62.5%) and widdra-2,4(14)-diene (9%) were the main components of P. spinosa aerial part essential oil, while elemicin (60.1%) and caryophyllene oxide (9.8%) were the main components of P. spinosa seed essential oil. α-Phellandrene (25.5%), p-cymene (15.3%), and limonene (13.3%) were found in P. flabellifolia essential oil. The inhibition zone diameters for P. flabellifolia essential oil were significantly higher than for the two other essential oils from P. spinosa (p<0.05). In broth dilution assay (µL/mL), the sensitive microorganism to Pycnocycla sp. (P. spinosa, P. flabellifolia) was Aspergillus niger, followed by Candida albicans. In 2,2-diphenyl-1-picrylhydrazyl (DPPH) system, P. spinosa aerial parts essential oil (IC50=548 µg/mL) had higher antioxidant activity than that of two other essential oils.

Download Full-text

Chemical Compositions and Biological Activities of Essential Oils of Beilschmiedia glabra

Natural Product Communications ◽

10.1177/1934578x1501000740 ◽

2015 ◽

Vol 10 (7) ◽

pp. 1934578X1501000 ◽

Cited By ~ 4

Author(s):

Wan Mohd Nuzul Hakimi Wan Salleh ◽

Farediah Ahmad ◽

Khong Heng Yen ◽

Razauden Mohamed Zulkifli

Keyword(s):

Essential Oils ◽

Phenolic Content ◽

Biological Activities ◽

Chemical Compositions ◽

Strong Activity ◽

Germacrene D ◽

Leaf Oil ◽

Weak Activity ◽

Anti Inflammatory ◽

Β Carotene

This study was designed to examine the chemical compositions of essential oils from Beilschmiedia glabra and their antioxidant, antimicrobial, antityrosinase, acetylcholinesterase and anti-inflammatory activities. In total, 47 components were identified in the essential oils, which made up 86.8% and 89.7% of the leaf and bark oils, respectively. The leaf oil is composed mainly of β-eudesmol (15.4%), β-selinene (12.2%), caryophyllene oxide (8.1%) and γ-gurjunene (5.2%), while the bark oil contains high percentages of β-eudesmol (19.3%), β-selinene (16.9%), δ-cadinene (15.8%), germacrene D (9.8%) and β-caryophyllene (5.5%). Antioxidant activity showed that the leaf oil has the highest phenolic content at 233.4 mg GA/g, while the bark oil showed potent activity in the β-carotene/linoleic acid bleaching assay. However, both oils showed weak activity in the DPPH and ABTS assays. For antimicrobial activity, the leaf and bark oils displayed strong activity against Candida glabrata and Saccharomyces cerevisiae with MIC values of 31.3 and 62.5 μg/mL, respectively. Percentage inhibitions against tyrosinase (leaf 73.7%; bark 76.0%) and acetylcholinesterase (leaf 48.1%; bark 45.2%) were tested at a concentration of 1 mg/mL, while anti-inflammatory activity (leaf 59.7%; bark 48.9%) was evaluated at a concentration of 100 μM. Evaluation of these assays indicated moderate levels of activity.

Download Full-text

Comparative Study of Volatile Components of Essential Oil from Conyza canadensis between Hydrodistillation and Steam Distillation

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.113-116.1644 ◽

2010 ◽

Vol 113-116 ◽

pp. 1644-1647 ◽

Cited By ~ 2

Author(s):

Zhi Ming Liu ◽

Hai Ying Wang ◽

Shan Shan Liu ◽

Nai Xiang Jiang

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Steam Distillation ◽

Biomimetic Synthesis ◽

Gas Chromatography Mass Spectrometry ◽

Volatile Components ◽

Conyza Canadensis ◽

Aerial Parts ◽

Germacrene D ◽

The Common

The volatile components of essential oils of fresh aerial parts from horseweed (Conyza canadensis) collected in October with hydrodistillation and steam distillation, respectively, were analyzed by gas chromatography-mass spectrometry. The results showed that limonene, β-pinene, trans-α-bergamotene, cis-β-ocimene, β-myrcene, germacrene D, 1,3,8-p-menthatriene, caryophyllene, (E)-β-farnesene, (Z)-β-farnesene, α-curcumene, caryophyllene oxide were the common volatile components in the essential oils between hydrodistillation and steam distillation. High limonene content (68.87%) was in the essential oil with hydrodistillation. Limonene or other terpenoids has the potential to develop as the biomimetic synthesis template for environmentally-friendly herbicide.

Download Full-text