Antifungal activity of Mentha piperita and Carum carvi essential oils

Aromatic plants are one of the most important sources of biologically active secondary metabolites, which possess various antimicrobial characteristics. The aim of this work was to examine the effect of antifungal activities of mint and caraway essential oils against the selected fungi. Eight species of molds were selected for antifungal testing: Alternaria alternata, Aspegillus flavus, A. niger, A. versicolor, Eurotium herbariorum, Penicillium aurantiogriseum, P. chrysogenum and P. expansum. Testing of essential oils antifungal activity against the selected species was conducted using the disc diffusion method by adding mint and caraway essential oils (0.5, 1, 5, and 10 ?l per disc). Antifungal activity of essential oils was expressed by the diameter of inhibition zone (mm). The most powerful effect of mint essential oil was recorded against E. herbariorum, as its growth was completely inhibited by the quantity of 5 ?l. The weakest inhibitory effect was observed against P. chrysogenum (inhibition zone 13.67 mm) by the quantity of 10 ?l. The most powerful antifungal activity of caraway was observed against E. herbariorum as growth was completely inhibited by the quantity of 10 ?l. The weakest inhibitory effect was observed against A. niger (inhibition zone 28 mm) by the quantity of 10 ?l.

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Note: Inhibition of Pathogenic Bacteria by Essential Oils at Different Concentrations

Food Science and Technology International ◽

10.1177/1082013203009002003 ◽

2003 ◽

Vol 9 (2) ◽

pp. 85-88 ◽

Cited By ~ 42

Author(s):

G. Özkan ◽

O. Sağdiç ◽

M. Özcan

Keyword(s):

Essential Oils ◽

Pathogenic Bacteria ◽

Antibacterial Agents ◽

Antimicrobial Effect ◽

Inhibitory Effect ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

E Coli ◽

Paper Disc

The antimicrobial effect of 11 selected Turkish spice essential oils was investigated against seventeen pathogenic bacteria. The antimicrobial activity of the essential oils of six spices (cumin, fennel, laurel, mint, marjoram, oregano, pickling herb, sage, savory, thyme (black) and thyme) was tested at four concentrations (0.2, 0.4, 1 and 2%) on various microorganisms ( E. aerogenes, E. coli, E. coli O157:H7, K. pneumoniae, P. vulgaris, S. enteritidis, S. gallinarum, S. typhimurium, S. aureus, Y. enterocolitica, A. hydrophila, C. xerosis, M. luteus, M. smegmatis, E. feacalis, P. aeruginosa and P. fluorescens). All preparations showed antibacterial activity against at least one or more bacteria. The inhibitory effect of the essential oils was evaluated through paper disc diffusion method. In general, the essential oils at 1 and 2% levels were effective. The most active essential oils were marjoram, thyme and oregano. According to the results, the studied essential oils potentially might be used as antibacterial agents to prevent the spoilage of food products, although further research is needed.

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Antifungal properties of Foeniculum vulgare, Carum carvi and Eucalyptus sp. essential oils against Candida albicans strains

Zbornik Matice srpske za prirodne nauke ◽

10.2298/zmspn1324195s ◽

2013 ◽

pp. 195-202 ◽

Cited By ~ 6

Author(s):

Jelica Skrobonja ◽

Dafina Delic ◽

Maja Karaman ◽

Milan Matavulj ◽

Mirjana Bogavac

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Essential Oils ◽

Inhibition Zone ◽

Biologically Active ◽

Foeniculum Vulgare ◽

Minimum Fungicidal Concentration ◽

Carum Carvi ◽

Microdilution Method

Aromatic plants are among the most important sources of biologically active secondary metabolites, with high antimicrobal potential. This study was carried out to examine in vitro antifungal activity of Foeniculum vulgare (Apiaceae), Carum carvi (Apiaceae) and Eucalyptus sp.(Myrtaceae) essential oils against three Candida albicans strains of different origin (laboratory-CAL, human pulmonary-CAH and ATCC10231-CAR). The essential oils were screened on C. albicans using disc and well-diffusion and microdilution method, and compared to Nystatine and Fluconazole as standard anti-mycotics. The activity of tested oils was expressed by inhibition zone diameter (mm), minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) (mg/ml). The results indicated that studied essential oils show antifungal activity against all three isolates of C. albicans. It was observed that each oil exhibits different degree of antifungal activity depending on the oil concentration applied as well as on analyzed strain of C. albicans. Carum carvi demonstrated the strongest antifungal effect to all tested strains, showing the lowest MIC values (0.03mg/ml for CAL, 0.06mg/ml for CAH, and 0.11mg/ml for CAR, respectively). Eucalyptus sp. exhibited the lowest antifungal activity, with MIC values ranging from 0.11 mg/ml for CAL to 0.45 mg/ml for both CAH and CAR.

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Antifungal Effect of Different Plant Extracts against Phytopathogenic Fungi Alternaria alternata and Fusarium oxysporum Isolated from Tomato Plant

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i31a31681 ◽

2021 ◽

pp. 188-197

Author(s):

Haytham M. Daradka ◽

Abdelrahman Saleem ◽

Wael Abdulghani Obaid

Keyword(s):

Fusarium Oxysporum ◽

Plant Extracts ◽

Alternaria Alternata ◽

Inhibition Zone ◽

Inhibitory Effect ◽

Phytopathogenic Fungi ◽

Biologically Active ◽

Diffusion Method ◽

Minimum Fungicidal Concentration ◽

Fungal Phytopathogens

The antifungal activity of 10 plant extracts was tested against the phytopathogenic fungi, Alternaria alternata and Fusarium oxysporum, the pathogens of early blight and wilt diseases of tomato. Plants tested related to different families. Three doses (10, 50 and 100 mg/ml) of plant extracts were assayed on A. alternata and F. oxysporum by measuring the inhibition zone of mycelial growth of fungi by disc-diffusion method on the Potato dextrose agar (PDA). The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of plant extracts were determined. The inhibitory effect of plant extracts ranged from 10-29 and 12-31 mm against A. alternata and F. oxysporum, respectively. The extracts of Pulicaria crispa and Olea chrysophylla were the most effective antifungal followed by Citrullus colocynthis, Psiadia arabica and Otostegia fruticosa. These plant extracts contain biologically active major compounds such as alkaloids, flavonoids, saponins, tannins and terpenoids. The remaining plant extracts did not emerge any inhibitive effect on tested fungi. The MIC and MFC of plant extracts ranged 6–38, 7-34 and 28-56, 22-50 mg/ml for A. alternata and F. oxysporum, respectively. Therefore, these plant extracts have biologically active compounds and have a potential to control fungal phytopathogens in agricultural crops.

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Antifungal activity of cultivated oyster mushrooms on various agro-wastes

Summa Phytopathologica ◽

10.1590/0100-5405/2069 ◽

2017 ◽

Vol 43 (1) ◽

pp. 9-13 ◽

Cited By ~ 7

Author(s):

Mustafa Nadhim Owaid ◽

Sajid Salahuddin Saleem Al-Saeedi ◽

Idham Ali Abed Al-Assaffii

Keyword(s):

Antifungal Activity ◽

Wheat Straw ◽

Fungal Pathogens ◽

Date Palm ◽

Inhibition Zone ◽

Diffusion Method ◽

Fruiting Bodies ◽

Disc Diffusion Method ◽

Date Palm Fibers

ABSTRACT This study evaluated the antifungal activity of four fruiting bodies of oyster mushroom harvested from three agro-substrates in vitro. At three concentrations (2, 4 and 8 mg/disc), extracts discs of Pleurotus ostreatus (grey), P. ostreatus var. florida, P. cornucopiae var. citrinopileatus and P. salmoneostramineus were tested against three fungal pathogens: Trichoderma harzianum (after 2 days), Verticillium sp. and Pythium sp. (after 5 days) via the Disc Diffusion Method. The highest overall activity was by the extract disc Y2 (P. cornucopiae grown on M2 substrate; 70% wheat straw, 20% hardwood sawdust and 10% date palm fibers) and the lowest by Y1 (P. cornucopiae grown on wheat straw). The best inhibition zone was 16 mm toward T. harzianum by extract disc W2 (2 mg/disc) (P. ostreatus var. florida grown on M2 substrate), compared with 23 mm with Nystatin disc (100 U), followed 7 and 5 mm by P3 (P. salmoneostramineus grown on M3 substrate; 50% wheat straw, 30% hardwood sawdust and 20% date palm fibers) extract disc (8 mg/disc) against Pythium sp., and (4 mg/disc) against Verticillium sp., respectively.

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Bactericidal And Antioxidant Effects of Domestic Essential Oils On Certain Pathogenic Bacteria.

10.21203/rs.3.rs-605851/v1 ◽

2021 ◽

Author(s):

Faisal MB. Al-Sarraj ◽

Ahmad M. Qumri

Keyword(s):

Antioxidant Activity ◽

Essential Oils ◽

Pathogenic Bacteria ◽

Inhibition Zone ◽

Diffusion Method ◽

Disc Diffusion ◽

Bacterial Strains ◽

Disc Diffusion Method ◽

Rapd Pcr

Abstract Background: Essential oils (EOs) are volatile, liquid substances that come from natural sources such as plants. As secondary metabolites of most plant species, that are typically found in leaf chloroplasts or from the hydrolysis of certain glycosides, their primary function is to combat attacks from predators as well as attract pollinators. Due to their biological activity, EOs have gained popularity in industries such as food processing, cosmetics, and personal care products as well as the pharmaceutical industry. In this study, the antimicrobial nature and antioxidant activity of Saudi Arabian EOs were compared with the antimicrobial activity of known antibiotics.Methods: The In vitro evaluation of antibacterial activity and antioxidant capacity of EOs collected from Jeddah domestic market for gram-negative and gram-positive pathogenic bacteria was carried out using the disc diffusion method. Disc diffusion method was used for the assessment of bactericidal activity of local EOs. The antioxidant activity was determined using phosphomolybdenum method. Furthermore, RAPD-PCR was used to compare the effects of these EOs on DNA in both untreated and treated pathogenic bacterial strains.Results and Conclusion: Bacterial strains with varying antibiotic resistance potential have been successfully isolated. The inhibition zone measurement revealed that the EOs used had some antibacterial effects on the bacterial strains as well as some selectivity on the bacterial strains that they acted on.

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AKTIVITAS ANTIJAMUR EKSTRAK ETANOL 50% DAN ETANOL 96% DAUN PACAR KUKU Lawsonia inermis L TERHADAP Trichophyton mentagrophytes

EKOLOGIA ◽

10.33751/ekol.v19i1.1657 ◽

2020 ◽

Vol 19 (1) ◽

pp. 12-19

Author(s):

Oom Komala ◽

Yulianita . ◽

Fuji Raka Siwi

Keyword(s):

Antifungal Activity ◽

Ethanol Extract ◽

Trichophyton Mentagrophytes ◽

Inhibition Zone ◽

Diffusion Method ◽

Dilution Method ◽

Disc Diffusion ◽

Lawsonia Inermis ◽

Disc Diffusion Method ◽

Mic Value

Trichopyton mentagrophytes is a type of fungus that belongs to a group of dermatophytes, and a disease that causes dermatophytosis (ringworm), this fungus likes parts that contain keratin substances such as skin, hair / hair, nails or horns. This study aims to determine the antifungal activity of 50% and 96% ethanol extract. KHM testing was made with a concentration of 2.5%, 5%, 7.5% and 10% with the dilution method to determine KHM. Whereas in testing the width of the inhibitory area (LDH) was carried out after getting the MIC value at concentrations of 10%, 25% and 50% with disc diffusion method to determine the effective LDH and qualitative phytochemical testing. The results showed that 96% ethanol solvent at a concentration of 50% could inhibit the fungus Trichopyton mentagrophytes compared to 50% ethanol as seen from the inhibition zone formed in the width of the inhibitory area. Secondary metabolite compounds found in 50% ethanol extract and 96% ethanol are flavonoids, alkaloids, saponins, tannins, quinones and terpenoids.

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Chemical composition, antibacterial and antioxidant activities of Juniperus oxycedrus essential oils from North Western Algeria

South Asian Journal of Experimental Biology ◽

10.38150/sajeb.7(2).p74-83 ◽

2018 ◽

Vol 7 (2) ◽

pp. 74-83

Author(s):

Cherifa Zahaf ◽

Djilali Achour ◽

Meriem Mokhtar ◽

Moussa Brada

Keyword(s):

Essential Oils ◽

Steam Distillation ◽

Antioxidant Activities ◽

Inhibition Zone ◽

Inhibitory Effect ◽

Juniperus Oxycedrus ◽

Germacrene D ◽

Western Algeria ◽

North Western ◽

Β Carotene

The aim of the current study was to identify the essential oils of Juniperus oxycedrus from North Western Algeria (Mostaganem) by gas chromatography coupled to mass spectrometer (GC-MS), and to evaluate their antibac-terial and antioxidant activities. Extraction was carried out by two processes, hydrodistillation (HD) and steam distillation (SD). Analysis by GC-MS led to the identification of 38 components using both methods. HD was found to be the best process for the extraction of Juniperus essential oil, and the ma-jor identified compounds were: germacrene-D (38.28%), followed by α.-pinene (17.66%), and δ-cadinene (5.91%). In the antibacterial test, SD oil had a higher inhibitory effect. The strain Staphylococcus aureus was the most sensitive with an inhibition zone of 15.25 mm and a minimum inhibitory concentration (MIC) of 0.25 mg/mL. The antioxidant activity of J. oxycedrus es-sential oils obtained by HD was more important than SD (IC50= 19.52 mg/mL and IC50= 38.62 mg/mL) with DPPH and β-carotene bleaching tests respec-tively. Essential oils of J. oxycedrus could therefore represent good candidates to be used as antioxidant and antibacterial agents.

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The Citrus reticulata essential oil: evaluation of antifungal activity against penicillium species related to bakery products spoilage

Potravinarstvo Slovak Journal of Food Sciences ◽

10.5219/1695 ◽

2021 ◽

Vol 15 ◽

pp. 1112-1119

Author(s):

Hana Ďúranová ◽

Veronika Valková ◽

Lucia Galovičová ◽

Jana Štefániková ◽

Miroslava Kačániová

Keyword(s):

Antioxidant Activity ◽

Antifungal Activity ◽

Essential Oil ◽

Vapor Phase ◽

Radical Scavenging ◽

Diffusion Method ◽

Wheat Bread ◽

Citrus Reticulata ◽

Disc Diffusion ◽

Disc Diffusion Method

Fungal food spoilage plays a key role in the deterioration of food products, and finding a suitable natural preservative can solve this problem. Therefore, antifungal activity of green mandarin (Citrus reticulata) essential oil (GMEO) in the vapor phase against the growth of Penicillium (P.) expansum and P. chrysogenum inoculated on wheat bread (in situ experiment) was investigated in the current research. The volatile compounds of the GMEO were analyzed by a gas chromatograph coupled to a mass spectrometer (GC–MS), and its antioxidant activity was determined by testing free radical-scavenging capacity (DPPH assay). Moreover, the disc diffusion method was used to analyze the antifungal activity of GMEO in in vitro conditions. The results demonstrate that the Citrus reticulata EO consisted of α-limonene as the most abundant component (71.5%), followed by γ-terpinene (13.9%), and β-pinene (3.5%), and it displayed the weak antioxidant activity with the value of inhibition 5.6 ±0.7%, which corresponds to 103.0 ±6.4 µg TEAC.mL-1. The findings from the GMEO antifungal activity determination revealed that values for the inhibition zone with disc diffusion method ranged from 0.00 ±0.00 (no antifungal effectiveness) to 5.67 ±0.58 mm (moderate antifungal activity). Finally, exposure of Penicillium strains growing on bread to GMEO in vapor phase led to the finding that 250 μL.L-1 of GMEO exhibited the lowest value for mycelial growth inhibition (MGI) of P. expansum (-51.37 ±3.01%) whose negative value reflects even supportive effect of the EO on the microscopic fungus growth. On the other hand, GMEO at this concentration (250 μL.L-1) resulted in the strongest inhibitory action (MGI: 54.15 ±1.15%) against growth of P. chrysogenum. Based on the findings it can be concluded that GMEO in the vapor phase is not an effective antifungal agent against the growth of P. expansum inoculated on bread; however, its antifungal potential manifested against P. chrysogenum suggests GMEO to be an appropriate alternative to the use of chemical inhibitors for bread preservation.

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