Total flavonoid fraction of rhizoma drynaria improves bone properties in ovariectomized mice and exerts estrogen-like activities in rat osteoblast-like (UMR-106) cells

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
W.-Y. Pang ◽  
X.-L. Wang ◽  
K.-C. Wong ◽  
P.-C. Leung ◽  
X.-S. Yao ◽  
...  
Keyword(s):  
Total Flavonoid ◽  
Ovariectomized Mice ◽  
Bone Properties ◽  
Rat Osteoblast ◽  
Flavonoid Fraction ◽  
Umr 106

scholarly journals Naringin improves bone properties in ovariectomized mice and exerts oestrogen-like activities in rat osteoblast-like (UMR-106) cells

2010 ◽  
Vol 159 (8) ◽  
pp. 1693-1703 ◽  
Author(s):  
Wai-Yin Pang ◽  
Xin-Lun Wang ◽  
Sau-Keng Mok ◽  
Wan-Ping Lai ◽  
Hung-Kay Chow ◽  
...  
Keyword(s):  
Ovariectomized Mice ◽  
Bone Properties ◽  
Rat Osteoblast ◽  
Umr 106

scholarly journals Drynaria fortunei-derived total flavonoid fraction and isolated compounds exert oestrogen-like protective effects in bone

2013 ◽  
Vol 110 (3) ◽  
pp. 475-485 ◽  
Author(s):  
Ka-Chun Wong ◽  
Wai-Yin Pang ◽  
Xin-Lun Wang ◽  
Sao-Keng Mok ◽  
Wan-Ping Lai ◽  
...  
Keyword(s):  
Chinese Herb ◽  
Osteoblastic Cell ◽  
Dependent Manner ◽  
Protective Effects ◽  
Receptor Activator ◽  
Rat Osteoblast ◽  
Flavonoid Fraction ◽  
Umr 106 ◽  
Dose Dependent ◽  
Tomography Analysis

Drynaria fortunei (Kunze) J. Sm. (DF), a Chinese herb commonly used for the treatment of bone fracture, was previously shown to exert anabolic effects on bone. However, its active ingredients as well as the mechanisms of action are far from clear. The present study aimed to characterise the bone anabolic effects of DF flavonoid fraction (DFTF) in ovariectomised (OVX) mice and to determine if DFTF and its isolated compounds exert oestrogen-like effects in rat osteoblast-like UMR-106 cells. Young OVX C57/BL6J mice were treated orally with DFTF (0·087, 0·173 or 0·346 mg/g per d), 17β-oestradiol (2 μg/g per d) or its vehicle for 6 weeks. Serum and urine samples were collected for biochemical marker analysis. Bones were collected for computed tomography analysis. UMR-106 cells were treated with DFTF and isolated compounds naringin, (2S)-5,7,3′,5′-tetrahydroxy-flavonone 7-O-neohesperidoside (compound 1) and 5,7-dihydroxychromone 7-O-neohesperidoside (compound 2). DFTF exerted dose-dependent effects in improving bone mineral densities as well as bone strength at the femur, tibia and lumbar spine L1 in OVX mice. DFTF and the three isolated compounds stimulated osteoblastic cell proliferation and alkaline phosphatase activities in a dose-dependent manner. In addition, they stimulated the ratio of osteoprotegrin and receptor-activator NF-κB ligand mRNA expression, suggesting their involvement in inhibiting osteoclastogenesis. These stimulatory effects on osteoblastic functions were abolished in the presence of oestrogen receptor (ER) antagonist, ICI 182780. The present results suggested that DFTF is effective in protecting against OVX-induced bone loss in mice, and its actions in regulating osteoblastic activities appear to be mediated by ER.

Na+/Ca2+ exchange in rat osteoblast-like UMR 106 cells

2009 ◽  
Vol 11 (11) ◽  
pp. 1666-1675 ◽  
Author(s):  
Kenneth E. White ◽  
Frank A. Gesek ◽  
Peter A. Friedman
Keyword(s):  
Rat Osteoblast ◽  
Umr 106

scholarly journals Improvement of bone properties and enhancement of mineralization by ethanol extract of Fructus Ligustri Lucidi

2008 ◽  
Vol 99 (3) ◽  
pp. 494-502 ◽  
Author(s):  
Yan Zhang ◽  
Ping-Chung Leung ◽  
Chun-Tao Che ◽  
Hung-Kay Chow ◽  
Chun-Fu Wu ◽  
...  
Keyword(s):  
Bone Loss ◽  
Bone Mineral ◽  
Sham Operation ◽  
Dependent Manner ◽  
Aged Rats ◽  
Mineral Density ◽  
Fructus Ligustri Lucidi ◽  
Bone Properties ◽  
Ovx Rats ◽  
Umr 106

Fructus Ligustri Lucidi (FLL), a kidney-tonifying Chinese herb, was shown to regulate Ca balance in ovariectomized (OVX) rats in our previous study. This study investigated whether it could improve bone properties in aged normal and OVX rats and increase osteoblastic differentiation in rat osteoblast-like UMR-106 cells. Ten-month-old aged rats underwent sham-operation or ovariectomy, were orally administered with FLL extracts or its vehicle and fed with diets containing different levels of Ca (LCD, 0·1 % Ca; MCD, 0·6 % Ca; HCD, 1·2 % Ca) for 12 weeks. Ovariectomy induced bone loss at multiple-sites of both tibia and femur in all rats being studied. FLL extract increased bone mineral density and bone mineral content at both tibial and femoral diaphysis as well as the lumbar vertebra (LV-2) in rats fed either LCD or MCD. In addition, FLL increased biomechanical strength of the tibial diaphysis in these rats. Combination of FLL and high-Ca diet significantly improved bone mass of cortical and trabecular bone at appendicular bones and LV-2 and decreased bone loss associated with ovarietomy and low-Ca feeding. Treatment of UMR-106 cells with FLL extracts accelerated the formation of calcified matrix and increased extracellular Ca and P depositions in time- and dose-dependent manner. The level of mineralization reached a maximum by 6 d incubation at the dosage of 10 μg FLL extract/ml. Our study indicated that FLL extract could improve bone properties in aged rats possibly via its direct action on osteoblastic cells by enhancement of the mineralization process.

scholarly journals Chlorate-induced inhibition of tyrosine sulfation on bone sialoprotein synthesized by a rat osteoblast-like cell line (UMR 106-01 BSP).

1994 ◽  
Vol 269 (7) ◽  
pp. 4845-4852
Author(s):  
K.P. Mintz ◽  
L.W. Fisher ◽  
W.J. Grzesik ◽  
V.C. Hascall ◽  
R.J. Midura
Keyword(s):  
Cell Line ◽  
Bone Sialoprotein ◽  
Tyrosine Sulfation ◽  
Rat Osteoblast ◽  
Umr 106

Er-Xian Decoction Exerts Estrogen-Like Osteoprotective EffectsIn VivoandIn Vitro

2014 ◽  
Vol 42 (02) ◽  
pp. 409-426 ◽  
Author(s):  
Ka-Chun Wong ◽  
Kin-Shing Lee ◽  
Hon-Kit Luk ◽  
Hoi-Ying Wan ◽  
Chui-Kwan Ho ◽  
...  
Keyword(s):  
Distal Femur ◽  
Sprague Dawley ◽  
Micro Computed Tomography ◽  
Beneficial Effects ◽  
Proliferative Effect ◽  
Bone Properties ◽  
Ovx Rats ◽  
Herba Epimedii ◽  
Umr 106

Er-xian Decoction (EXD), containing Herba epimedii Maxim (HEP) and Curculigo orchioides Gaertn (XM) as principal drugs, is a traditional Chinese medicine (TCM) formula prescribed for the treatment of postmenopausal osteoporosis. In the present study, the in vivo anti-osteoporosis effects of EXD, HEP and XM on four-month-old ovariectomized (OVX) Sprague–Dawley rats were investigated. Micro-computed tomography analysis showed that EXD could significantly improve the micro-architectural parameters (BMD, BV/TV, Tb.N, Tb.Th, and Tb.Sp) of trabecular bone in the distal femur and proximal tibia in OVX rats (p < 0.05). The biomechanical parameters of the distal femur in rats treated with EXD were also improved significantly (p < 0.05 vs. OVX group). The in vivo efficacy of EXD was found to be superior to HEP or XM alone in improving the bone properties of OVX rats. Treatment of rat osteoblastic-like UMR-106 cells with EXD, HEP, and XM significantly promoted the cell proliferation rate (p < 0.05) with the most promising effects observed in cells treated with EXD (p < 0.001). The proliferative effect in UMR-106 cells induced by EXD, HEP, and XM were abolished in the presence of the estrogen antagonist, ICI182780, suggesting that their effects were mediated by estrogen receptor (ER). Additionally, EXD could activate ER-α and ER-β mediated estrogen-response element (ERE)-dependent luciferase activity as well as phosphorylate ER-α at serine 118 in UMR-106 cells. Taken together, EXD offered better osteoprotective effects than its single principal herb, and the beneficial effects of EXD in preventing bone deteriorations are, at least partially, through the ER signaling pathway.

scholarly journals Bone-protective effects of bioactive fractions and ingredients in Sambucus williamsii HANCE

2011 ◽  
Vol 106 (12) ◽  
pp. 1802-1809 ◽  
Author(s):  
Hui-Hui Xiao ◽  
Yi Dai ◽  
Hoi-Ying Wan ◽  
Man-Sau Wong ◽  
Xin-Sheng Yao
Keyword(s):  
Crude Extract ◽  
Protective Effects ◽  
Mice Model ◽  
Mineral Density ◽  
Hplc Fingerprint ◽  
Bone Properties ◽  
Ovx Rats ◽  
Proliferation And Differentiation ◽  
Bone Content ◽  
Umr 106

Our previous study demonstrated that 60 % ethanol crude extract of Sambucus williamsii HANCE (SWH) improved bone mass, bone strength and bone micro-structure in both ovariectomised (OVX) rats and mice. The present study aims to identify the bioactive fractions and ingredients in SWH that account for its osteoprotective effects. Bilateral sham-operated mice acted as controls. OVX C57BL/6J mice, aged 12 weeks, were orally administrated daily with vehicle or 17β-oestradiol (3·2 mg/kg), SWH (60 % ethanol crude extract; 1·0 g/kg), SWA (water eluate; 0·570 g/kg), SWB (30 % ethanol eluate; 0·128 g/kg) or SWC (50 and 95 % ethanol eluates; 0·189 g/kg) for 12 weeks. The effects of the different fractions on bone properties in the OVX mice model were studied. In addition, their effects on osteoblast proliferation and differentiation were evaluated in UMR 106 cells. SWC significantly restored bone mineral density and improved bone size and bone content parameters in the femur and tibia as well as increased biomechanical strength at the tibia diaphysis in OVX mice. Similarly, SWC was the most potent fraction in stimulating cell proliferation and differentiation in UMR 106 cells. Also, SWC did not alter uterus weight in OVX mice. Nine major peaks, seven lignans and two phenolic acids, in the HPLC fingerprint of the SWC fraction were identified, isolated and characterised. In conclusion, the present study demonstrated that SWC was the most potent fraction in SWH that exerted anti-osteoporotic effects. Furthermore, lignans might be the potential bioactive components in SWC.

Purification of bone sialoprotein from the medium of the rat osteoblast-like cell line UMR 106-01 BSP

1994 ◽  
Vol 16 (3-4) ◽  
pp. 205-209 ◽  
Author(s):  
K. P. Mintz ◽  
R. J. Midura ◽  
L. W. Fisher
Keyword(s):  
Cell Line ◽  
Bone Sialoprotein ◽  
Rat Osteoblast ◽  
Umr 106
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