Genetic State of Pulsatilla Patens (L.) Mill. Coenopopulations Based on DNA Labeling

AbstractNontraditional irrigation water sources (e.g., recycled water, brackish water) may harbor human pathogens, including Vibrio spp., that could be present in a viable-but-nonculturable (VBNC) state, stymieing current culture-based detection methods. To overcome this challenge, we coupled 5-bromo-2′-deoxyuridine (BrdU) labeling, enrichment techniques, and 16S rRNA sequencing to identify metabolically-active Vibrio spp. in nontraditional irrigation water (recycled water, pond water, non-tidal freshwater, and tidal brackish water). Our coupled BrdU-labeling and sequencing approach revealed the presence of metabolically-active Vibrio spp. at all sampling sites. Whereas, the culture-based method only detected vibrios at three of the four sites. We observed the presence of V. cholerae, V. vulnificus, and V. parahaemolyticus using both methods, while V. aesturianus and V. shilonii were detected only through our labeling/sequencing approach. Multiple other pathogens of concern to human health were also identified through our labeling/sequencing approach including P. shigelloides, B. cereus and E. cloacae. Most importantly, 16S rRNA sequencing of BrdU-labeled samples resulted in Vibrio spp. detection even when our culture-based methods resulted in negative detection. This suggests that our novel approach can effectively detect metabolically-active Vibrio spp. that may have been present in a VBNC state, refining our understanding of the prevalence of vibrios in nontraditional irrigation waters.

Download Full-text

Extracts from Pulsatilla patens target cancer-related signaling pathways in HeLa cells

Scientific Reports ◽

10.1038/s41598-021-90136-3 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Grażyna Łaska ◽

Magdalena Maciejewska-Turska ◽

Elwira Sieniawska ◽

Łukasz Świątek ◽

David S. Pasco ◽

...

Keyword(s):

Signaling Pathways ◽

Hela Cells ◽

Luciferase Reporter ◽

Cervical Cancer Cell Line ◽

Phytochemical Analysis ◽

Root Extract ◽

Triterpenoid Saponins ◽

Cancer Signaling ◽

Methanolic Extracts ◽

Pulsatilla Patens

AbstractThe purpose of this study was to determine if a methanolic extract of the Pulsatilla patens (L.) Mill. can inhibit the progression of cancer through the modulation of cancer-related metabolic signaling pathways. We analyzed a panel of 13 inducible luciferase reporter gene vectors which expression is driven by enhancer elements that bind to specific transcription factors for the evaluation of the activity of cancer signaling pathways. The root extract of P. patens exhibited strong inhibition of several signaling pathways in HeLa cells, a cervical cancer cell line, and was found to be the most potent in inhibiting the activation of Stat3, Smad, AP-1, NF-κB, MYC, Ets, Wnt and Hdghog, at a concentration of 40 µg/mL. The methanolic extracts of P. patens enhanced apoptotic death, deregulated cellular proliferation, differentiation, and progression towards the neoplastic phenotype by altering key signaling molecules required for cell cycle progression. This is the first study to report the influence of Pulsatilla species on cancer signaling pathways. Further, our detailed phytochemical analysis of the methanolic extracts of the P. patens allowed to deduce that compounds, which strongly suppressed the growth and proliferation of HeLa cancer cells were mainly triterpenoid saponins accompanied by phenolic acids.

Download Full-text

Cell cycle-dependent effects on deoxyribonucleotide and DNA labeling by nucleoside precursors in mammalian cells.

Molecular and Cellular Biology ◽

10.1128/mcb.7.1.532 ◽

1987 ◽

Vol 7 (1) ◽

pp. 532-534 ◽

Cited By ~ 22

Author(s):

J M Leeds ◽

C K Mathews

Keyword(s):

Cell Cycle ◽

Mammalian Cells ◽

Chinese Hamster Ovary Cells ◽

Chinese Hamster ◽

G1 Phase ◽

Ovary Cells ◽

Dna Labeling ◽

Metabolic Pool ◽

Specific Activities ◽

Cycle Dependent

dCTP pools equilibrated to equivalent specific activities in Chinese hamster ovary cells or in nuclei after incubation of cells with radiolabeled nucleosides, indicating that dCTP in nuclei does not constitute a distinct metabolic pool. In the G1 phase, [5-3H]deoxycytidine labeled dCTP to unexpectedly high specific activities. This may explain reports of replication-excluded DNA precursor pools.

Download Full-text

Correlated autoradiographic and biochemical study of DNA labeling in equine abortion virus hepatitis

Virology ◽

10.1016/0042-6822(62)90227-1 ◽

1962 ◽

Vol 16 (4) ◽

pp. 460-465 ◽

Cited By ~ 7

Author(s):

Glenn A. Gentry ◽

Barbara M. Walker ◽

Charles C. Randall

Keyword(s):

Biochemical Study ◽

Virus Hepatitis ◽

Dna Labeling ◽

Equine Abortion

Download Full-text

Petrophytic-steppe communities of the Mogutova Mountain (Zhiguli Hills)

Samara Journal of Science ◽

10.17816/snv20163110 ◽

2016 ◽

Vol 5 (3) ◽

pp. 48-53

Author(s):

Larisa Valerievna Sidyakina ◽

Vladimir Mikhailovich Vasjukov ◽

Sergey Vladimirovich Saksonov

Keyword(s):

Russian Federation ◽

Endemic Species ◽

National Park ◽

The North ◽

Steppe Communities ◽

Feather Grass ◽

The Russian Federation ◽

Pulsatilla Patens ◽

Steep Slopes

The Mogutova Mountain - domal erosive ostanets in the north of the Zhigulyovsk hill, lies in national park Samara Luka. Communities petrophytic steppes are formed on calcareous exposures of steep slopes of the western, southern and east expositions of the mountain Mogutova. Petrophytic-steppe communities on a slope of east exposition are described - petrophytic-forbs-feather grass steppe ( Stipa capillata + Stipa pennata + Herbae stepposae ), on a slope of west exposition - petrophytic-sunrose-feather grass steppe ( Stipa pennata + Helianthemum nummularium ), on a slope of south exposition - petrophytic-thyme-sedge-feather grass steppe ( Stipa pulcherrima + Carex pediformis + Thymus zheguliensis ). In the studied communities three narrow-local of endemic species of the Zhigulyovsk hill grows: Euphorbia zhiguliensis, Gypsophila juzepczukii, Thymus zheguliensis and three of subendemic species grows: Festuca wolgensis s.str. , Tanacetum sclerophyllum (endemics of Central Volga area), Koeleria sclerophylla (endemic of Central Volga area and South Ural); the relict element of flora is presented by three species: Alyssum lenense, Clausia aprica, Helianthemum nummularium . Five species are included in the Red book of the Russian Federation: Astragalus zingeri, Euphorbia zhiguliensis, Koeleria sclerophylla, Stipa pennata, Thymus zheguliensis ; sixteen species are included in the Red book of the Samara region: Adonanthe vernalis, Alyssum lenense, Astragalus zingeri, Clausia aprica, Cotoneaster laxiflorus, Euphorbia zhiguliensis, Festuca wolgensis, Gypsophila juzepczukii, Helianthemum nummularium, Koeleria sclerophylla, Onosma volgensis, Pulsatilla patens, Scabiosa isetensis, Stipa pennata, Tanacetum sclerophyllum, Thymus zheguliensis. The most widespread plants of petrophytic steppes of the Mogutova Mountain: Astragalus zingeri, Carex pediformis, Echinops ruthenicus, Galium hexanarium, Gypsophila juzepczukii, Helictotrichon desertorum, Jurinea ledebourii, Onosma volgensis, Psephellus carbonatus, Stipa pennata, Tanacetum sclerophyllum, Thesium ramosum, Thymus zheguliensis .

Download Full-text