scholarly journals Topical treatment of Klebsiella pneumoniae B5055 induced burn wound infection in mice using natural products

2010 ◽  
Vol 4 (06) ◽  
pp. 367-377 ◽  
Author(s):  
Seema Kumari ◽  
Kusum Harjai ◽  
Sanjay Chhibber
Keyword(s):  
Klebsiella Pneumoniae ◽  
Wound Infection ◽  
Antimicrobial Agents ◽  
Aloe Vera ◽  
Burn Wound ◽  
Additional Advantage ◽  
Specific Phage ◽  
Aloe Vera Gel ◽  
Therapeutic Value ◽  
Daily Application

Background: Burn wound infection remains the principal cause of death in burn patients. Efficacy of honey and aloe vera gel was evaluated in the treatment of burn wound infection induced with Klebsiella pneumoniae B5055 and their efficacy was compared with an isolated and well-characterized Klebsiella specific phage Kpn5. Methodology: A full thickness burn wound was induced in mice and infected with K. pneumoniae B5055 via topical route. The efficacy of natural antimicrobial agents (honey and aloe vera gel) topically applied daily was compared with the efficacy of phage Kpn5 suspended in hydrogel applied topically a single time on the burn wound. Efficacy of these antimicrobial agents was assessed on the basis of the percentage of infected mice that survived following treatment. Results: In comparison to untreated control mice, those treated with a single dose of phage Kpn5 at MOI of 200 showed significant reduction in mortality (P < 0.001). Daily application of honey and aloe vera gel provided significant protection (P < 0.001), but in combination with phage, no additional advantage was observed (P > 0.05) compared to the use of honey and aloe vera gel alone. Conclusions: The results of this study strongly suggest that phage Kpn5 has therapeutic value in treating burn wound infection in mice as a single topical application of this phage was able to rescue mice from infection caused by K. pneumoniae B5055 in comparison to multiple applications of honey and aloe vera gel.

Bacteriophage versus antimicrobial agents for the treatment of murine burn wound infection caused by Klebsiella pneumoniae B5055

2011 ◽  
Vol 60 (2) ◽  
pp. 205-210 ◽  
Author(s):  
Seema Kumari ◽  
Kusum Harjai ◽  
Sanjay Chhibber
Keyword(s):  
Klebsiella Pneumoniae ◽  
Wound Infection ◽  
Silver Nitrate ◽  
Antimicrobial Agents ◽  
Phage Therapy ◽  
Burn Wound ◽  
Wound Site ◽  
Two Agents ◽  
Specific Phage ◽  
Percentage Survival

This study was planned to evaluate the efficacy of silver nitrate and gentamicin in the treatment of burn wound infection and to compare it with phage therapy using an isolated and well-characterized Klebsiella-specific phage, Kpn5. A full-thickness burn wound was induced in mice and infected with Klebsiella pneumoniae B5055 via the topical route. Different concentrations of silver nitrate or gentamicin were applied topically daily after establishment of infection. Phage Kpn5 mixed in hydrogel was also applied topically at an m.o.i. of 200 on the burn wound site. The efficacy of these antimicrobial agents was assessed on the basis of percentage survival of infected mice following treatment. The results showed that a single dose of phage Kpn5 resulted in a significant reduction in mortality (P<0.001). Daily applications of silver nitrate and gentamicin at 0.5 % and 1000 mg l−1, respectively, provided significant protection (P<0.001) compared to lower concentrations of the two agents. However, the level of protection given by these two agents was lower than that given by the phage therapy. The results strongly suggest that phage Kpn5 has therapeutic utility in treating burn wound infection in mice as a single topical application of this phage was able to rescue mice from infection caused by K. pneumoniae B5055 in comparison to multiple applications of silver nitrate and gentamicin.

scholarly journals Antibacterial Activity of Aloe vera Gel against Multidrug Resistant Staphylococcus aureus and Pseudomonas aeruginosa

2020 ◽  
pp. 74-80
Author(s):  
Usman A. ◽  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Wound Infection ◽  
Aloe Vera ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Diffusion Technique ◽  
Aloe Vera Gel

Staphylococcus aureus and Pseudomonas aeruginosa have been implicated as important nosocomial pathogens causing severe infections especially in hospitalized patients. The aim of the study was to assess the antibacterial activity of Aloe vera gel against multidrug resistant S. aureus and P. aeruginosa isolated from wound. Clinical isolates of S. aureus and P. aeruginosa from wound infection were collected from Microbiology laboratory of Barau Dikko Teaching Hospital (BDTH), Kaduna and re-confirmed using standard microbiological procedure. Antibiotic susceptibility pattern of the isolates was determined using Kirby Bauer disk diffusion method. Aloe vera gel was obtained fresh matured leaves of Aloe vera plant and was screened for the presence of phytochemical constituents. Antibacterial activity of the Aloe vera gel against Multidrug Resistant (MDR) Staphylococcus aureus and Pseudomonas aeruginosa isolates was determined by agar well diffusion technique. The result revealed that all the two isolates were resistant to more than three classes of antibiotics. The Staphylococcus aureus isolate was resistant to fluoroquinolone (ciprofloxacin), aminoglycoside (gentamicin), cephalosporin (cefaroline), folate pathway antagonist (trimethoprim-sulfamethoxazole), penicillin(cefoxitin) and macrolide (erythromycin) while the Pseudomonas aeruginosa isolate was resistant to βlactam (ticarcillin-clavulanate), fluoroquinolone (ciprofloxacin and norfloxacin), aminoglycoside (gentamicin) and cephalosporin (ceftazidime)hence regarded as MDR isolates. Phytochemical screening of the gel revealed the presence of saponins, flavonoids, terpenoids and alkaloids. The Aloe vera gel was found to have antibacterial activity against the test isolates with MIC and MBC values of 25µg/mL and 50µg/mL against MDR S. aureus 50µg/mL and 100µg/mL against MDR P. aeruginosa respectively. The study identified that Aloe vera gel possesses antibacterial activity against MDR S. aureus and P. aeruginosa isolated from wound infection. Keywords: Aloe vera gel, Staphylococcus aureus, Pseudomonas aeruginosa, antibacterial, MDR

In Vivo Comparison of Two Silver Sulfadiazine Antimicrobial Agents on Burn Wound Infection

1987 ◽  
Vol 8 (5) ◽  
pp. 381-383 ◽  
Author(s):  
Vic Velanovich ◽  
John P. Heggers ◽  
Martin C. Robson ◽  
Steven M. Zoellner ◽  
Thomas P. McHugh ◽  
...  
Keyword(s):  
Wound Infection ◽  
Antimicrobial Agents ◽  
Silver Sulfadiazine ◽  
Burn Wound

Effective Topical Delivery of H-AgNPs for Eradication of Klebsiella pneumoniae–Induced Burn Wound Infection

2019 ◽  
Vol 20 (5) ◽  
Author(s):  
Sanjay Chhibber ◽  
Vijay Singh Gondil ◽  
Love Singla ◽  
Munish Kumar ◽  
Tanya Chhibber ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Wound Infection ◽  
Topical Delivery ◽  
Burn Wound

scholarly journals Postharvest Application of Aloe vera Gel-Based Edible Coating to Improve the Quality and Storage Stability of Fresh-Cut Papaya

2020 ◽  
Vol 2020 ◽  
pp. 1-10 ◽  
Author(s):  
Vittorio Farina ◽  
Roberta Passafiume ◽  
Ilenia Tinebra ◽  
Dario Scuderi ◽  
Filippo Saletta ◽  
...  
Keyword(s):  
Sodium Alginate ◽  
Antimicrobial Agents ◽  
Aloe Vera ◽  
Titratable Acidity ◽  
Solid Content ◽  
Soluble Solid Content ◽  
Aloe Vera Gel ◽  
Soluble Solid ◽  
Fresh Cut ◽  
Ripening Rate

Ready-to-eat products are damaged by various factors, including exposure to O2 and CO2, extreme temperatures, and rapid decay, due to trauma during processing. The use of natural antimicrobial agents and antioxidants might extend the shelf-life of the fruits. The aim of this work is to investigate the effects of four different antibrowning and gelling agents added into the Aloe vera gel-based edible coatings and applied to fresh-cut papaya. EC1 treatment consists of Aloe vera gel (30% v/v), EC2 contains CaCl2 (5% v/v), EC3 contains K carrageenan (0.5% v/v), and EC4 contains sodium alginate (1.5% v/v) and K carrageenan (0.5% v/v). The fruits treated with EC2 showed the best results while maintaining high values in terms of firmness (that differ from the control of 42.5%), soluble solid content (that differ from the control of 14.6%), and titratable acidity (that differ from the control of 49%). Hence, the addition of CaCl2 also reduces the ripening rate and loss of color without altering the product’s sensory qualities. EC3 and EC4 treatments have provided an oxygen barrier and reduced respiratory rate, increasing the firmness retention and keeping a high C∗ value thanks to K carrageenan and sodium alginate.

scholarly journals Aloe vera Gel: Effective Therapeutic Agent against Multidrug-Resistant Pseudomonas aeruginosa Isolates Recovered from Burn Wound Infections

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Mehdi Goudarzi ◽  
Maryam Fazeli ◽  
Mehdi Azad ◽  
Sima Sadat Seyedjavadi ◽  
Reza Mousavi
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Therapeutic Agent ◽  
Biological Activities ◽  
Aloe Vera ◽  
Multidrug Resistant ◽  
Wound Infections ◽  
Burn Wound ◽  
Aloe Vera Gel ◽  
Herbal Medicinal

Objective. Aloe vera is an herbal medicinal plant with biological activities, such as antimicrobial, anticancer, anti-inflammatory, and antidiabetic ones, and immunomodulatory properties. The purpose of this study was investigation of in vitro antimicrobial activity of A. vera gel against multidrug-resistant (MDR) Pseudomonas aeruginosa isolated from patients with burn wound infections. Methods. During a 6-month study, 140 clinical isolates of P. aeruginosa were collected from patients admitted to the burn wards of a hospital in Tehran, Iran. Antimicrobial susceptibility test was carried out against the pathogens using the A. vera gel and antibiotics (imipenem, gentamicin, and ciprofloxacin). Results. The antibiogram revealed that 47 (33.6%) of all isolates were MDR P. aeruginosa. The extract isolated from A. vera has antibacterial activity against all of isolates. Also, 42 (89.4%) isolates were inhibited by A. vera gel extract at minimum inhibitory concentration (MIC) ≤ 200 µg/mL. MIC value of A. vera gel for other isolates (10.6%) was 800 µg/mL. All of MDR P. aeruginosa strains were inhibited by A. vera at similar MIC50 and MIC90 200 µg/mL. Conclusion. Based on our results, A. vera gel at various concentrations can be used as an effective antibacterial agent in order to prevent wound infection caused by P. aeruginosa.

scholarly journals Comparison of the effectiveness of Aloe Vera Gel with 2% Nitrofurazone ointment on the healing of superficial second-degree burns. Randomised clinical trial

2020 ◽  
Author(s):  
Shokoh Varaei ◽  
Hadi Ranjbar ◽  
Parichehr Sabaghzadeh ◽  
Sanaz Bostani ◽  
Soodabhe Amirsalari
Keyword(s):  
Clinical Trial ◽  
Assessment Tool ◽  
Aloe Vera ◽  
The Body ◽  
Burn Wound ◽  
Aloe Vera Gel ◽  
Degree Burn ◽  
Significant Difference ◽  
The Mean ◽  
Second Degree

Abstract Background Burn injuries are one of the most common sources of trauma globally that comprise a significant drain on long-term personal and healthcare cost Large surface area burn wounds are difficult to manage and may result in significant physiologic and psychological sequelae . The aim of this study was to compare the effectiveness of Aloe Vera gel with Nitrofurazone ointment in the healing of superficial second-degree burn wounds.Methods The present study was a split body controlled, randomized clinical trial. The sample was recruited from patients with superficial second-degree burn wound who were prescribed to treat with 2% Nitrofurazone ointment. Thirty patients with at least two burn, each burn on an alternate side of the body, entered the study — samples allocated to two groups which received Aloe Vera gel or 2% Nitrofurazone ointment on their burns. Bates-Jensen Wound assessment tool (BWAT) was used to evaluate the healing of burns. The burns were evaluated before, one, two and three weeks after the beginning of treatment.Results The mean ± SD of BWAT scores in Aloe Vera zones were 30.32 ± 3.28, 27.33 ± 3.38, 21.33 ± 3.13, 16.12 ± 2.16 respectively (F(2, 65.07) =440.00, p=0.001). The mean ± SD of BWAT scores in Nitrofurazone ointment zones were 30.51 ± 3.79, 28.45 ± 3.49, 23.36 ± 2.89, 19.23 ± 2.11 (F(1, 52.00) =228.00, p=0.001).Conclusions There is a significant difference in (BWAT) scores between intervention and control groups. Aloe Vera gel was as effective as Nitrofurazone ointment in the treatment of superficial second-degree burns.Trial registration IRCT2014113020151N1. Registered 14 December 2014, https://www.irct.ir/trial/17874

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