scholarly journals Correlation analysis of levels of inflammatory cytokines and nitric oxide in peripheral blood with urine proteins and renal function in patients with gestational hypertension

2018 ◽  
Author(s):  
Jing-Yang Zhang ◽  
Xiao-Xiao Cao ◽  
Hong-Xia Wen ◽  
Hong-Yan Zhang
Keyword(s):  
Nitric Oxide ◽  
Renal Function ◽  
Correlation Analysis ◽  
Inflammatory Cytokines ◽  
Peripheral Blood ◽  
Gestational Hypertension ◽  
Urine Proteins

EFFECTS OF TARAXACUM MONGOLICUM EXTRACT ON LIPOPOLYSACCHARIDE-INDUCED NITRIC OXIDE AND CYTOKINES PRODUCTION BY BOVINE PERIPHERAL BLOOD MONONUCLEAR CELLS

2016 ◽  
Vol 42 (04) ◽  
pp. 203-212
Author(s):  
Kuo-Hua Lee ◽  
Kai-Chen Hsu ◽  
Yu-Shan Wang ◽  
Chia-Chou Yeh ◽  
Jih-Yih Chen ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Mrna Expression ◽  
Inflammatory Cytokines ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Bovine Mastitis ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Pro Inflammatory Cytokines

The objective of this study is to investigate Taraxacum mongolicum (TM) as a therapeutic alternative for preventing and treating bovine mastitis. The effect of the anti-inflammatory activity of Taraxacum mongolicum extract (TME) on lipopolysaccharide (LPS)-induced responses was studied in the bovine peripheral blood mononuclear cells (PBMCs). The dried plant TM was extracted with 10 volumes of distilled water to generate its water extract. PBMCs were pretreated with various concentrations of TME (0, 1, 10, 100, 1000 [Formula: see text]g/mL) and subsequently incubated with LPS (1 [Formula: see text]g/mL). Cell viability was analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylthiazolium bromide (MTT) assay. The level of nitric oxide (NO) was determined by using Griess reagent assay. The mRNA expression levels of pro-inflammatory cytokines including interleukin (IL)-1[Formula: see text], IL-6, IL-8, tumor necrosis factor (TNF)-[Formula: see text] and granulocyte chemotactic protein (GCP)-2 were determined by using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The results showed no significant cytotoxic effects on the PBMCs at various treated concentrations of TME. Treatment of TME (100 and 1000 [Formula: see text]g/mL) significantly inhibited NO production in LPS-stimulated PBMCs. TME (10 [Formula: see text]g/mL) significantly inhibited LPS-stimulated IL-1[Formula: see text], IL-6, IL-8, TNF-[Formula: see text] and GCP-2 mRNA expression in PBMCs at a time-dependent manner. In this article, we reported for the first time that TME significantly inhibited production of NO and pro-inflammatory cytokines in LPS-stimulated PBMCs. This finding could be useful for clinical practice in preventing and treating bovine mastitis.

scholarly journals Intrauterine Growth Restriction: Cytokine Profiles of Trophoblast Antigen-Stimulated Maternal Lymphocytes

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Raj Raghupathy ◽  
Majedah Al-Azemi ◽  
Fawaz Azizieh
Keyword(s):  
Inflammatory Cytokines ◽  
Peripheral Blood ◽  
Intrauterine Growth Restriction ◽  
Mononuclear Cells ◽  
Normal Pregnancy ◽  
Placental Insufficiency ◽  
Growth Restriction ◽  
Intrauterine Growth ◽  
Anti Inflammatory ◽  
Ifnγ And Tnfα

Intrauterine growth restriction (IUGR) is an important perinatal syndrome that poses several serious short- and long-term effects. We studied cytokine production by maternal peripheral blood lymphocytes stimulated by trophoblast antigens. 36 women with a diagnosis of IUGR and 22 healthy women with normal fetal growth were inducted. Peripheral blood mononuclear cells were stimulated with trophoblast antigens and levels of the proinflammatory cytokines IL-6, IL-8, IL-12, IL-23, IFNγ, and TNFα and the anti-inflammatory cytokines IL-4, IL-10, and IL-13 were measured in culture supernatants by ELISA. IL-8 was produced at higher levels by blood cells of the IUGR group than normal pregnant women, while IL-13 was produced at lower levels. IL-8, IFNγ, and TNFα were higher in IUGR with placental insufficiency than in normal pregnancy. IL-12 levels were higher and IL-10 levels were lower in IUGR with placental insufficiency than in IUGR without placental insufficiency. We suggest that a stronger pro-inflammatory bias exists in IUGR as compared to normal pregnancy and in IUGR with placental insufficiency when compared to IUGR without placental insufficiency. Several ratios of proinflammatory to anti-inflammatory cytokines also support the existence of an inflammatory bias in IUGR.

scholarly journals Systemic hemodynamics and renal function in hemorrhaged dogs resuscitated with cross-linked hemoglobin

2000 ◽  
Vol 278 (1) ◽  
pp. R28-R33 ◽  
Author(s):  
John M. Stulak ◽  
Luis A. Juncos ◽  
John A. Haas ◽  
J. Carlos Romero
Keyword(s):  
Nitric Oxide ◽  
Renal Function ◽  
Cardiac Output ◽  
Sodium Excretion ◽  
Systemic Hemodynamics ◽  
Urinary Flow ◽  
Excretory Function ◽  
Renal Function Recovery ◽  
Function Recovery ◽  
Hormonal Milieu

Cross-linked hemoglobin (XL-Hb) infused into dogs increases mean arterial pressure (MAP) but decreases blood flow to the renal (RBF), mesenteric (MBF), and iliac (IBF) circulations. These actions differ markedly from dextran infusion (which increases RBF, MBF, and IBF without altering MAP) and may be due to scavenging of nitric oxide by XL-Hb. However, because the hormonal milieu regulating regional circulation is altered during hemorrhage (when XL-Hb may be used), we studied whether systemic hemodynamics, RBF, MBF, IBF, and renal excretory function in hemorrhaged dogs was altered when resuscitated with XL-Hb compared with dextran ( n = 6 each). Hemorrhage decreased MAP by 25% due to a 75% decline in cardiac output. RBF, MBF, and IBF all fell by 33, 64, and 72%, respectively ( P < 0.05 each). There was also a fall in glomerular filtration rate (GFR), urinary flow, and sodium excretion ( P < 0.05 each). After resuscitation, MAP, cardiac output, RBF, MBF, IBF, and GFR all recovered to basal values with either XL-Hb or dextran. Urinary flow and sodium excretion increased to above basal levels with dextran (both by 3.5-fold; P < 0.05) or XL-Hb (by 7.5- and 10-fold, respectively; P < 0.05). We conclude that resuscitation with XL-Hb after hemorrhage not only increases MAP, but also restores RBF, MBF, IBF, GFR, and urinary sodium and volume excretion analogously to dextran. The results contrast with those in normal dogs and suggest that nitric oxide inhibition does not impair hemodynamic and renal function recovery during hemorrhage.

Hypothermia Induces Anti-Inflammatory Cytokines and Inhibits Nitric Oxide and Myeloperoxidase-Mediated Damage in the Hearts of Endotoxemic Rats

CHEST Journal ◽  
2004 ◽  
Vol 125 (4) ◽  
pp. 1483-1491 ◽  
Author(s):  
Philip O. Scumpia ◽  
Paul J. Sarcia ◽  
Kindra M. Kelly ◽  
Vincent G. DeMarco ◽  
Jeffrey W. Skimming
Keyword(s):  
Nitric Oxide ◽  
Inflammatory Cytokines ◽  
Anti Inflammatory

Enteral nutrition affects nitric oxide production in peripheral blood and liver after a postoperative lipopolysaccharide-induced endotoxemia in rats

Nutrition ◽  
2007 ◽  
Vol 23 (7-8) ◽  
pp. 575-581 ◽  
Author(s):  
George Kolios ◽  
Katerina Kotzampassi ◽  
Pinelopi Manousou ◽  
Daniel Paramythiotis ◽  
Helen Papanastasiou ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Enteral Nutrition ◽  
Peripheral Blood ◽  
Nitric Oxide Production ◽  
Oxide Production

Effects of NG-nitro-L-arginine methyl ester on renal function and blood pressure

1991 ◽  
Vol 261 (6) ◽  
pp. F1033-F1037 ◽  
Author(s):  
V. Lahera ◽  
M. G. Salom ◽  
F. Miranda-Guardiola ◽  
S. Moncada ◽  
J. C. Romero
Keyword(s):  
Nitric Oxide ◽  
Blood Pressure ◽  
Renal Function ◽  
Methyl Ester ◽  
Renal Plasma Flow ◽  
Urinary Sodium Excretion ◽  
Systemic Blood Pressure ◽  
Synthesis Inhibitor ◽  
Renal Changes ◽  
Dose Dependent

The dose-dependent effects of intravenous infusions of nitric oxide (NO) synthesis inhibitor, NG-nitro-L-arginine methyl ester (L-NAME; 0.1, 1, 10, and 50 micrograms.kg-1.min-1), were studied in anesthetized rats to determine whether the inhibitory actions of L-NAME are manifested primarily in alterations of renal function or whether they are the consequences of the increase in systemic blood pressure. Mean arterial pressure (MAP) was not altered by the intravenous L-NAME infusions of 0.1 and 1.0 microgram.kg-1.min-1. However, 0.1 microgram.kg-1.min-1 L-NAME induced a 30% decrease in urine flow rate (UV). The administration of 1.0 microgram.kg-1.min-1 L-NAME, in addition to decreasing UV, also decreased urinary sodium excretion (UNaV) and renal plasma flow (RPF). The intravenous L-NAME infusions of 10.0 and 50.0 microgram.kg-1.min-1 intravenous L-NAME infusions of 10.0 and 50.0 microgram.kg-1.min-1 produced significant increases in MAP that reversed the initial fall in UV and UNaV, despite decreasing RPF and glomerular filtration rate (GFR). The administration of L-arginine alone (10 micrograms.kg-1.min-1) did not modify any of the parameters measured, but it effectively prevented all the hemodynamic and renal changes induced by the infusion of 50 micrograms.kg-1.min-1 L-NAME. These results suggest that the decrease in nitric oxide production induced by the intravenous infusion of L-NAME affects renal excretion of sodium and water in the absence of any significant change in blood pressure. At larger doses, L-NAME also produces hypertension that overrides the initial antinatriuretic effect.

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