Clinical significance of miR‑181a in patients with neonatal sepsis and its regulatory role in the lipopolysaccharide‑induced inflammatory response

Neonatal sepsis (NS) occurs in neonates within 28 days, especially preterm infants. The dysregulation of miRNAs is widely detected in NS. The study investigated the expression changes and clinical significance of miR-129-5p in NS patients and further explored the regulatory role of miR-129-5p in the LPS-induced inflammatory response in monocytes. A total of 75 neonates with NS and 84 neonates without NS were recruited. qRT-PCR was used for the measurement of miR-129-5p expression. The receiver operating characteristic (ROC) curve was constructed for diagnostic value analysis. ELISA was used to detect the concentration of inflammatory cytokines. Monocytes were isolated from the blood of neonates to investigate the role of miR-129-5p in the LPS-induced inflammatory response in vitro. miR-129-5p was low expressed in the serum of NS cases compared with controls. Serum miR-129-5p had a diagnostic value for NS with a sensitivity of 82.7% and specificity of 79.8%. There was close association for serum miR-129-5p with TNF-α (r = -0.652, p < 0.001) and IL-8 (r = -0.700, p < 0.001) levels in NS patients. Overexpression of miR-129-5p reversed the increasing trend of TNF-α and IL-8 induced by LPS, whereas miR-129-5p downregulation aggravated the increase of TNF-α and IL-8 induced by LPS in monocytes. MiR-129-5p was downregulated in the serum of NS patients, and it might be a promising biomarker for disease diagnosis. Overexpression of miR-129-5p alleviated the inflammatory response of NS.

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Clinical significance of miR-19b-3p in patients with sepsis and its regulatory role in the LPS-induced inflammatory response

European Journal of Medical Research ◽

10.1186/s40001-020-00408-3 ◽

2020 ◽

Vol 25 (1) ◽

Cited By ~ 2

Author(s):

Huimin Xu ◽

Xiuwu Liu ◽

Huaijun Ni

Keyword(s):

Inflammatory Response ◽

Clinical Significance ◽

Regulatory Role

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The Relationship Among Intra-Amniotic Inflammatory Response, The Progression of Inflammation in Chorionic Plate and Early-Onset Neonatal Sepsis

Frontiers in Pediatrics ◽

10.3389/fped.2021.582472 ◽

2021 ◽

Vol 9 ◽

Author(s):

Kyung Chul Moon ◽

Jeong-Won Oh ◽

Chan-Wook Park ◽

Joong Shin Park ◽

Jong Kwan Jun

Keyword(s):

Inflammatory Response ◽

Neonatal Sepsis ◽

Early Onset ◽

Multiple Logistic Regression Analysis ◽

Intrauterine Infection ◽

Preterm Neonates ◽

Chorionic Plate ◽

Study Population ◽

Stage 1 ◽

The Relationship

Background: The chorionic plate (CP) has been denigrated by the well-known route of the extraplacental membranes from the decidua parietalis through the chorion to the amnion in the progression of ascending intrauterine infection among preterm births (PTBs). However, considering previous studies reporting the relationship among intra-amniotic inflammatory response (IAIR), the progression of inflammation in extraplacental membranes and early-onset neonatal sepsis (EONS), and the anatomic connection between extraplacental membranes and CP, there is a good chance that IAIR would be more likely and severe according to the progression of inflammation in CP, and this progression of inflammation in CP would be associated with a significant increase in EONS in neonates delivered due to either PTL or preterm-PROM. Unfortunately, there is no information about the relationship among IAIR, the progression of inflammation in CP, and EONS among spontaneous PTBs. The objective of the current study is to examine this issue.Method: The study population included 309 singleton pregnant women-delivered preterm neonates with the following conditions: (1) gestational age (GA) at delivery: 20.0~36.9 weeks; (2) spontaneous PTBs: PTL (151 cases) or preterm-PROM (158 cases); (3) available results of placental histologic examination; (4) without congenital anomaly; and (5) delivery within 60 h of amniocentesis. We examined IAIR, and the frequency of intra-amniotic inflammation (IAI) and EONS according to the progression of inflammation in CP [i.e., stage-0, inflammation-free CP; stage-1, inflammation restricted to subchorionic fibrin (SCF); stage-2, inflammation in connective tissue (CT) of CP but without chorionic vasculitis; and stage-3, chorionic vasculitis]. IAIR was determined by amniotic fluid (AF) matrix metalloproteinase-8 (MMP-8) concentration (ng/ml), and IAI was defined as an elevated AF MMP-8 concentration (≥23 ng/ml). EONS included either suspected or proven EONS.Results: (1) Each stage (stage-0 to stage-3) was present in 69.3% (214/309), 15.9% (49/309), 11.0% (34/309), and 3.9% (12/309) of the study population. (2) AF MMP-8 concentrations continuously elevated according to the progression of inflammation in CP [stage-0 vs. stage-1 vs. stage-2 vs. stage-3; median (ng/ml), range (ng/ml); 6.0 (0.3–4202.7) vs. 153.9 (0.3–6142.6) vs. 464.9 (5.8–3929.0) vs. 1,780.4 (35.1–5019.5); Kruskal–Wallis test, P < 0.001 and Spearman's rank-correlation test, P < 0.000001, r = 0.553]. (3) Moreover, the frequency of IAI and EONS gradually increased with the progression of inflammation in CP [stage-0 vs. stage-1 vs. stage-2 vs. stage-3; IAI, 30.5% (64/210) vs. 70.2% (33/47) vs. 96.7% (29/30) vs. 100% (12/12); EONS, 3.5% (7/200) vs. 25.5% (12/47) vs. 32.3% (10/31) vs. 40.0% (4/10); each for Pearson's chi-square test, P < 0.000001 and linear-by-linear association, P < 0.000001]. (4) Of note, multiple logistic regression analysis demonstrated that a more advanced stage in the progression of inflammation within CP was associated with a higher odds ratio (OR) for EONS [stage-1 vs. stage-2 vs. stage-3; OR, 7.215, 95% confidence-interval (CI) (2.177–23.908) vs. OR, 10.705, 95% CI (2.613–43.849) vs. OR, 27.189, 95% CI (2.557–289.124)] compared with stage-0 even after the adjustment for potential confounding variables.Conclusion: IAIR is more likely and severe according to the progression of inflammation in CP, and this progression of inflammation in CP is an independent risk factor for EONS in spontaneous PTBs. This finding suggests that CP may be another playground for the progression of ascending intrauterine infection in addition to extraplacental membranes, and the progression of inflammation in CP may be used for the prediction of EONS in spontaneous PTBs.

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Two Cu(II)-based coordination polymers constructed from the pyridyl-carboxylate ligands: structural diversity and treatment activity on neonatal sepsis by reducing the over inflammatory response

Inorganic and Nano-Metal Chemistry ◽

10.1080/24701556.2020.1726956 ◽

2020 ◽

Vol 50 (9) ◽

pp. 853-858

Author(s):

Zhuo-Chao Lv ◽

Wen-Xiao Zhang ◽

Hong-Yan Zhao

Keyword(s):

Inflammatory Response ◽

Coordination Polymers ◽

Neonatal Sepsis ◽

Structural Diversity ◽

Carboxylate Ligands ◽

Treatment Activity

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Low-mannose-binding lectin levels in susceptibility to neonatal sepsis in preterm neonates with fetal inflammatory response syndrome

The Journal of Maternal-Fetal & Neonatal Medicine ◽

10.3109/14767050903551418 ◽

2010 ◽

Vol 23 (9) ◽

pp. 1009-1013 ◽

Cited By ~ 13

Author(s):

Ozgur Ozdemir ◽

Ener Cagri Dinleyici ◽

Neslihan Tekin ◽

Omer Colak ◽

M. Arif Aksit

Keyword(s):

Inflammatory Response ◽

Neonatal Sepsis ◽

Mannose Binding Lectin ◽

Preterm Neonates ◽

Mannose Binding ◽

Binding Lectin ◽

Fetal Inflammatory Response

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Erratum to : Clinical Significance Of HMGB-1 Levels In Patients With Systemic Inflammatory Response Syndrome

Nihon Kyukyu Igakukai Zasshi ◽

10.3893/jjaam.15.578 ◽

2004 ◽

Vol 15 (10) ◽

pp. 578-578

Keyword(s):

Systemic Inflammatory Response Syndrome ◽

Inflammatory Response ◽

Clinical Significance ◽

Systemic Inflammatory Response

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Clinical Significance of Variable Histomorphologic Findings Related to Mucosal Inflammation in Negative Appendectomy

Journal of Clinical Medicine ◽

10.3390/jcm10174030 ◽

2021 ◽

Vol 10 (17) ◽

pp. 4030

Author(s):

Euno Choi ◽

Youngeun Yoo ◽

Ji Min Kim ◽

Sun Hee Sung ◽

Dakeun Lee ◽

...

Keyword(s):

Abdominal Pain ◽

Inflammatory Response ◽

Clinical Significance ◽

Neutrophil Count ◽

Risk Group ◽

Signs And Symptoms ◽

Mucosal Inflammation ◽

Negative Appendectomy ◽

High Power Field ◽

Histopathologic Findings

The aim of the study was to investigate the clinical significance of various histomorphologic findings related to mucosal inflammation in negative appendectomy. We reviewed histopathologic findings of 118 negative appendectomies and correlated them with the appendicitis inflammatory response (AIR) score and appendiceal diameter. Among 118 patients with negative appendectomy, 94 (80%), 73 (78%) and 89 (75%) patients displayed mucosal inflammation, high neutrophil score (neutrophil count ≥10/5 high power field and surface epithelial flattening, respectively. Out of 118 patients with negative appendectomy, mucosal inflammation, high neutrophil score and surface epithelial flattening were associated with higher risk group according to the appendicitis inflammatory response (AIR) score (p < 0.05, respectively). In addition, mucosal inflammation, high neutrophil score and surface epithelial flattening were frequently detected in 118 negative appendectomies, compared with 24 incidental appendectomies (p < 0.05, respectively). In an analysis of 77 negative appendectomy patients with appendiceal diameter data available, increased appendiceal diameter was positively correlated with luminal inflammation, high neutrophil score and surface epithelial flattening (p < 0.05, respectively). In conclusion, mucosal inflammation, high neutrophil score and surface epithelial flattening in negative appendectomy may be relevant to patients’ signs and symptoms, especially in cases with no other cause of the abdominal pain.

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Clinical significance of D-dimer level in refractory Mycoplasma pneumoniae pneumonia

10.21203/rs.3.rs-42480/v3 ◽

2020 ◽

Author(s):

Xia Huang ◽

Dan Li ◽

Feng Liu ◽

Deyu Zhao ◽

Yifan Zhu ◽

...

Keyword(s):

Logistic Regression ◽

Inflammatory Response ◽

Clinical Significance ◽

Mycoplasma Pneumoniae ◽

Multivariate Logistic Regression Analysis ◽

Multivariate Logistic Regression ◽

Good Ability ◽

Excessive Inflammatory Response ◽

Mycoplasma Pneumoniae Pneumonia ◽

D Dimer

Abstract Background: The levels of serum D-dimer (D-D) in children with Mycoplasma pneumoniae pneumonia (MPP) were assessed to explore the clinical significance of D-D levels in refractory MPP (RMPP). Method: A total of 430 patients with MPP were enrolled between January 2015 and December 2015 and divided into a general MPP (GMPP) group (n=306) and a RMPP group (n=124). Clinical data, D-D level, white blood cell (WBC) count, proportion of neutrophils (N%), C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), alanine transaminase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) were compared between the two groups. Multivariate logistic regression was performed to identify independent predictors of RMPP. Results: (1) Hospitalization time, preadmission fever duration, total fever duration, WBC, N %, CRP, LDH, ESR, ALT, AST, and D-D were significantly higher in the RMPP group than those in the GMPP group (all P<0.05). (2) Correlation analysis showed that D-D was positively correlated with WBC, CRP, ESR, and LDH, and could be used to jointly evaluate the severity of the disease. (3) Multivariate logistic regression analysis identified preadmission fever duration, CRP, LDH and DD as independent risk factors for RMPP (all P<0. 05). D-D had the highest predictive power for RMPP (P<0.01). The D-D level also had a good ability to predict pleural effusion and liver injury (all P<0.01). Conclusion: Serum D-D levels were significantly increased in patients with RMPP, indicating that excessive inflammatory response and vascular endothelial injury with prolonged duration existed in this patient population. Increased levels of serum D-D may be used as an early predictor of RMPP and the occurrence of complications. Our findings provide a theoretical basis for the early diagnosis of RMPP, early intervention and excessive inflammatory response in the pathogenesis of mycoplasma.

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Clinical significance of D-dimer level in refractory Mycoplasma pneumoniae pneumonia

10.21203/rs.3.rs-42480/v2 ◽

2020 ◽

Author(s):

Xia Huang ◽

Dan Li ◽

Feng Liu ◽

Deyu Zhao ◽

Yifan Zhu ◽

...

Keyword(s):

Logistic Regression ◽

Inflammatory Response ◽

Clinical Significance ◽

Mycoplasma Pneumoniae ◽

Multivariate Logistic Regression Analysis ◽

Multivariate Logistic Regression ◽

Good Ability ◽

Wbc Count ◽

Excessive Inflammatory Response ◽

D Dimer

Abstract Background: The levels of serum D-dimer (D-D) in children with Mycoplasma pneumoniae pneumonia (MPP) were assessed to explore the clinical significance of D-D levels in refractory MPP (RMPP).Method: A total of 430 patients with MPP were enrolled between January 2015 and December 2015 and divided into a general MPP (GMPP) group (n=306) and a RMPP group (n=124). Clinical data, D-D level, white blood cell (WBC) count, proportion of neutrophils (N%), C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), alanine transaminase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) were compared between the two groups. Multivariate logistic regression was performed to identify independent predictors of RMPP.Results: (1) Hospitalization time, preadmission fever duration, total fever duration, WBC, N %, CRP, LDH, ESR, ALT, AST, and D-D were significantly higher in the RMPP group than those in the GMPP group, (all P<0.05). (2) Correlation analysis showed that D-D was positively correlated with WBC, CRP, ESR, and LDH, and could be used to jointly evaluate the severity of the disease. (3) Multivariate logistic regression analysis identified preadmission fever duration, CRP, LDH and DD as independent risk factors for RMPP (all P<0. 05). D-D had the highest predictive power for RMPP (P<0.01).. The D-D level also had a good ability to predict pleural effusion and liver injury (all P<0.01). Conclusion: Serum D-D levels were significantly increased in patients with RMPP, indicating that excessive inflammatory response and vascular endothelial injury with prolonged duration existed in this patient population. Increased levels of serum D-D may be used as an early predictor of RMPP and the occurrence of complications. Our findings provide a theoretical basis for the early diagnosis of RMPP, early intervention and excessive inflammatory response in the pathogenesis of mycoplasma.

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