scholarly journals Reprogrammed CRISPR‑Cas13a targeting the HPV16/18 E6 gene inhibits proliferation and induces apoptosis in E6‑transformed keratinocytes

2020 ◽  
Author(s):  
Chunjing Li ◽  
Liwen Guo ◽  
Guoqing Liu ◽  
Mingjuan Guo ◽  
Huiling Wei ◽  
...  
Keyword(s):  
E6 Gene

High E6 Gene Expression Predicts for Distant Metastasis and Poor Survival in Patients With HPV-Positive Oropharyngeal Squamous Cell Carcinoma

2016 ◽  
Vol 95 (4) ◽  
pp. 1132-1141 ◽  
Author(s):  
Shariq S. Khwaja ◽  
Callie Baker ◽  
Wesley Haynes ◽  
Christopher R. Spencer ◽  
Hiram Gay ◽  
...  
Keyword(s):  
Gene Expression ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Distant Metastasis ◽  
Oropharyngeal Squamous Cell Carcinoma ◽  
Poor Survival ◽  
E6 Gene

Cloning and sequencing of the murine farnesyltransferase α-encoding cDNA from a cell line which expresses the human papillomavirus type-16 E6 gene

Gene ◽  
1995 ◽  
Vol 164 (2) ◽  
pp. 373-374 ◽  
Author(s):  
Hiroshi Shirasawa ◽  
Tomoaki Kinoshita ◽  
Yuji Shino ◽  
Kohji Mori ◽  
Kumiko Shimizu ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Cell Line ◽  
Cloning And Sequencing ◽  
Human Papillomavirus Type 16 ◽  
E6 Gene ◽  
A Cell

The E6 gene of HPV 16 is sufficient for colony formation in primary human keratinocytes

1995 ◽  
Vol 121 (S1) ◽  
pp. S24-S24
Author(s):  
L. Sherman ◽  
R. Schlegel
Keyword(s):  
Colony Formation ◽  
Human Keratinocytes ◽  
Primary Human Keratinocytes ◽  
Hpv 16 ◽  
E6 Gene

Selective enhancement of bovine papillomavirus type 1 DNA replication in Xenopus laevis eggs by the E6 gene product

1989 ◽  
Vol 9 (2) ◽  
pp. 406-414
Author(s):  
H Romanczuk ◽  
W M Wormington
Keyword(s):  
Dna Replication ◽  
Xenopus Laevis ◽  
Gene Product ◽  
Mammalian Cells ◽  
Xenopus Laevis Oocytes ◽  
Bovine Papillomavirus ◽  
In Vitro Synthesis ◽  
E6 Gene ◽  
E6 Protein

Genetic analyses of bovine papillomavirus type 1 (BPV-1) DNA in transformed mammalian cells have indicated that the E6 gene product is essential for the establishment and maintenance of a high plasmid copy number. In order to analyze the direct effect of the E6 protein on the replication of a BPV-1-derived plasmid, a cDNA containing the BPV-1 E6 open reading frame was subcloned into an SP6 vector for the in vitro synthesis of the corresponding mRNA. The SP6 E6 mRNA was injected into Xenopus laevis oocytes to determine the subcellular localization of the E6 gene product and to analyze the effect of the protein on BPV-1 DNA replication. SP6 E6 mRNA microinjected into stage VI oocytes was translated into a 15.5-kilodalton protein that was specifically immunoprecipitated by antibodies directed against the E6 gene product. The E6 protein preferentially accumulated in oocyte nuclei, a localization which is consistent with the replicative functions in which it has been implicated. The expression of E6 in replication-competent mature oocytes selectively enhanced the replication of a BPV-derived plasmid, indicating a direct role for this gene product in the control of BPV-1 DNA replication.

Human papillomavirus type 16 E6 gene variations in Chinese population

2010 ◽  
Vol 36 (2) ◽  
pp. 160-163 ◽  
Author(s):  
H.B. Cai ◽  
C.C. Chen ◽  
X.H. Ding
Keyword(s):  
Human Papillomavirus ◽  
Chinese Population ◽  
Human Papillomavirus Type 16 ◽  
E6 Gene

scholarly journals Transcriptional Activation of the Telomerase hTERT Gene by Human Papillomavirus Type 16 E6 Oncoprotein

2001 ◽  
Vol 75 (9) ◽  
pp. 4467-4472 ◽  
Author(s):  
Tim Veldman ◽  
Izumi Horikawa ◽  
J. Carl Barrett ◽  
Richard Schlegel
Keyword(s):  
Human Papillomavirus ◽  
Transcriptional Activation ◽  
Transcriptional Control ◽  
Regulatory Region ◽  
Rnase Protection ◽  
Hpv 16 ◽  
Htert Gene ◽  
Human Papillomavirus Type 16 ◽  
E6 Gene

ABSTRACT The E6 and E7 oncogenes of human papillomavirus type 16 (HPV-16) are sufficient for the immortalization of human genital keratinocytes in vitro. The products of these viral genes associate with p53 and pRb tumor suppressor proteins, respectively, and interfere with their normal growth-regulatory functions. The HPV-16 E6 protein has also been shown to increase the telomerase enzyme activity in primary epithelial cells by an unknown mechanism. We report here that a study using reverse transcription-PCR and RNase protection assays in transduced primary human foreskin keratinocytes (HFKs) shows that the E6 gene (but not the E7 gene) increases telomerase hTERT gene transcription coordinately with E6-induced telomerase activity. In these same cells, the E6 gene induces a 6.5-fold increase in the activity of a 1,165-bp 5′ promoter/regulatory region of the hTERT gene, and this induction is attributable to a minimal 251-bp sequence (−211 to +40). Furthermore, there is a 35-bp region (+5 to +40) within this minimal E6-responsive promoter that is responsible for 60% of E6 activity. Although the minimal hTERT promoter contains Myc-responsive E-box elements and recent studies have suggested a role for Myc protein in hTERT transcriptional control, we found no alterations in the abundance of either c-Myc or c-Mad in E6-transduced HFKs, suggesting that there are other or additional transcription factors critical for regulating hTERT expression.

Effects of HPV Pseudotype Virus in Cutting E6 Gene Selectively in SiHa Cells

2018 ◽  
Vol 38 (2) ◽  
pp. 212-221 ◽  
Author(s):  
Yan-xiang Cheng ◽  
Gan-tao Chen ◽  
Xiao Yang ◽  
Yan-qing Wang ◽  
Li Hong
Keyword(s):  
E6 Gene ◽  
Siha Cells ◽  
Pseudotype Virus

scholarly journals Epithelial to Mesenchymal Transition (EMT) in a Laryngeal Squamous Cell Carcinoma of a Horse: Future Perspectives

Animals ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2318
Author(s):  
Federico Armando ◽  
Francesco Godizzi ◽  
Elisabetta Razzuoli ◽  
Fabio Leonardi ◽  
Mario Angelone ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Tumor Cells ◽  
Squamous Cell ◽  
Epithelial To Mesenchymal Transition ◽  
Hpv Infection ◽  
Mesenchymal Transition ◽  
Human Squamous Cell Carcinoma ◽  
E6 Gene ◽  
Twist 1

Squamous cell carcinoma (SCC) is one of the most frequent tumors of skin and muco-cutaneous junctions in the horse. Equine papillomavirus type 2 (EcPV2) has been detected in equine SCC of the oral tract and genitals, and recently also in the larynx. As human squamous cell carcinoma of the larynx (SCCL), it is strongly etiologically associated with high-risk papillomavirus (h-HPV) infection. This study focuses on tumor cells behavior in a naturally occurring tumor that can undergo the so-called epithelial to mesenchymal transition (EMT). A SCCL in a horse was investigated by immunohistochemistry using antibodies against E-cadherin, pan-cytokeratin AE3/AE1, β-catenin, N-cadherin, vimentin, ZEB-1, TWIST, and HIF-1α. EcPV2 DNA detection and expression of oncogenes in SCC were investigated. A cadherin switch and an intermediate filaments rearrangement within primary site tumor cells together with the expression of the EMT-related transcription factors TWIST-1, ZEB-1, and HIF-1α were observed. DNA obtained from the tumor showed EcPV2 positivity, with E2 gene disruption and E6 gene dysregulation. The results suggest that equine SCCL might be a valuable model for studying EMT and the potential interactions between EcPV2 oncoproteins and the EMT process in SCCL.

An improved method for primary culture of normal cervical epithelial cells and establishment of cell model in vitro with HPV-16 E6 gene by lentivirus

2017 ◽  
Vol 233 (4) ◽  
pp. 2773-2780 ◽  
Author(s):  
Tingting Fan ◽  
Xiaofu Li ◽  
Ya Li ◽  
Yanfang Zhi ◽  
Shouhua Rong ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Primary Culture ◽  
Cell Model ◽  
Improved Method ◽  
Hpv 16 ◽  
E6 Gene ◽  
Cervical Epithelial Cells

Human Papillomavirus Type 16 E6 Gene Variations in Young Chinese Women With Cervical Squamous Cell Carcinoma

2010 ◽  
Vol 18 (4) ◽  
pp. 406-412 ◽  
Author(s):  
Yan Hu ◽  
Yan-Ying Zhu ◽  
Sheng-Hui Zhang ◽  
Hua Zhu ◽  
Ci-Xia Shuai
Keyword(s):  
Squamous Cell Carcinoma ◽  
Human Papillomavirus ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Chinese Women ◽  
Cervical Squamous Cell Carcinoma ◽  
Human Papillomavirus Type 16 ◽  
E6 Gene
Sign in / Sign up

Export Citation Format

Share Document