Human tumor cell infection by Newcastle Disease Virus leads to upregulation of HLA and cell adhesion molecules and to induction of interferons, chemokines and finally apoptosis

2002 ◽  
Author(s):  
B. Washburn ◽  
V. Schirrmacher
Keyword(s):  
Cell Adhesion ◽  
Tumor Cell ◽  
Newcastle Disease Virus ◽  
Adhesion Molecules ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Cell Adhesion Molecules ◽  
Human Tumor ◽  
Cell Infection ◽  
Human Tumor Cell

scholarly journals Effect of interferon on the development of Trypanosoma cruzi in tissue culture "Vero" cells

1980 ◽  
Vol 75 (1-2) ◽  
pp. 157-160 ◽  
Author(s):  
R. R. Golgher ◽  
M. S. M. Bertelli ◽  
M. L. Petrillo-Peixoto ◽  
Z. Brener
Keyword(s):  
Tissue Culture ◽  
Trypanosoma Cruzi ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Vero Cells ◽  
Cell Infection ◽  
Amniotic Cells

Results are presented on the effects of interferon on the intracellular stages of T. cruzi in tissue culture "Vero" cells. Interferon was obtained by infecting monolayers of human amniotic cells with inactivated Newcastle disease virus. Interferon has not affected the cell infection by T. cruzi culture infective stages and neither has it prevented the transformation of amastigote into trypomastigote stages.

scholarly journals L1 cell adhesion molecules as regulators of tumor cell invasiveness

2009 ◽  
Vol 3 (3) ◽  
pp. 275-277 ◽  
Author(s):  
Priscila F. Siesser ◽  
Patricia F. Maness
Keyword(s):  
Cell Adhesion ◽  
Tumor Cell ◽  
Adhesion Molecules ◽  
Cell Adhesion Molecules ◽  
Cell Invasiveness

Newcastle disease virus therapy of human tumor xenografts: antitumor effects of local or systemic administration

2001 ◽  
Vol 172 (1) ◽  
pp. 27-36 ◽  
Author(s):  
Anan Phuangsab ◽  
Robert M. Lorence ◽  
Kirk W. Reichard ◽  
Mark E. Peeples ◽  
Robert J. Walter
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Human Tumor ◽  
Systemic Administration ◽  
Antitumor Effects ◽  
Human Tumor Xenografts ◽  
Tumor Xenografts

scholarly journals Safety and Clinical Usage of Newcastle Disease Virus in Cancer Therapy

2011 ◽  
Vol 2011 ◽  
pp. 1-13 ◽  
Author(s):  
Han Yuen Lam ◽  
Swee Keong Yeap ◽  
Mehdi Rasoli ◽  
Abdul Rahman Omar ◽  
Khatijah Yusoff ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Human Tumor ◽  
Wild Type ◽  
Anticancer Effect ◽  
Human Tumor Cells ◽  
Immunological Effects ◽  
Oncolytic Effect

Newcastle disease virus (NDV) is an avian virus that causes deadly infection to over 250 species of birds, including domestic and wild-type, thus resulting in substantial losses to the poultry industry worldwide. Many reports have demonstrated the oncolytic effect of NDV towards human tumor cells. The interesting aspect of NDV is its ability to selectively replicate in cancer cells. Some of the studies have undergone human clinical trials, and favorable results were obtained. Therefore, NDV strains can be the potential therapeutic agent in cancer therapy. However, investigation on the therapeutic perspectives of NDV, especially human immunological effects, is still ongoing. This paper provides an overview of the current studies on the cytotoxic and anticancer effect of NDV via direct oncolysis effects or immune stimulation. Safety of NDV strains applied for cancer immunotherapy is also discussed in this paper.

scholarly journals Adaptation of the Newcastle Disease Virus to Cell Cultures for Enhancing Its Oncolytic Properties

Acta Naturae ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 66-73
Author(s):  
K. S. Yurchenko ◽  
Yi. Jing ◽  
A. M. Shestopalov
Keyword(s):  
Newcastle Disease Virus ◽  
Cancer Cells ◽  
Tumor Cells ◽  
Disease Virus ◽  
Cell Cultures ◽  
Newcastle Disease ◽  
Human Cancer ◽  
Genetic Material ◽  
Human Tumor ◽  
Natural Strains

This study focuses on the adaptation of natural Newcastle disease virus (NDV) strains isolated from wild birds to human tumor cells. Many candidates for virotherapy are viruses pathogenic for human. During recombination of genetic material, there always exists a risk of getting a virus with an unstable genome. This problem can be solved by using natural apathogenic viruses as oncolytic agents. The Newcastle disease virus is the causative agent of contagious avian diseases. Its natural strains exhibit an antitumor effect and are considered safe for humans. As shown in earlier studies, the oncolytic properties of natural strains can be enhanced during adaptation to cell cultures, without interference in the virus genome. This study demonstrates that serial passaging increases the viral infectious titer in cancer cells. Moreover, the viability of tumor cells decreases post-infection when Newcastle disease virus strains are adapted to these cell cultures. The findings of this study complement the well-known data on the adaptation of the Newcastle disease virus to human cancer cells. Hence, it is possible to obtain a NDV strain with a more pronounced oncolytic potential during adaptation. This should be taken into account when choosing a strategy for designing anticancer drugs based on this virus.

Possible viral oncolysis by members of the Reoviridae and Paramyxoviridae families (an in vitro study).

2019 ◽  
Vol 37 (15_suppl) ◽  
pp. e14212-e14212
Author(s):  
Anastasia O. Sitkovskaya ◽  
Elena Yu. Zlatnik ◽  
Sergey A. Kolpakov ◽  
Elena P. Kolpakova ◽  
Inna A. Novikova ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Cell Lines ◽  
Newcastle Disease ◽  
Positive Control ◽  
Genetic Characteristics ◽  
Oncolytic Activity

e14212 Background: Our purpose was to study possible oncolytic activity of Newcastle disease virus and reovirus in different tumor cell cultures in vitro. Methods: The study was performed on LaSota strain Newcastle disease virus (NDV) vaccine (OOO NPP AVIVAK, 5000 doses) and human reovirus strains R-92 (3 serotype) deposited in the State collection of viruses of the Research Institute of Virology named after. D.I.Ivanovsky. HeLa (cervical cancer), A549 (lung adenocarcinoma), BT20 (breast cancer), PC3 (prostate cancer), HT29 (colon cancer) cell lines were used; pig embryo kidney cell culture (PEVKC) was used as a positive control. Results: After Newcastle disease virus was placed in the medium with tumor cell lines, a 5-day visual microscopic control of the cell morphology did not show signs of the cytopathic action, while a complete lysis of cell biomass was observed in the positive control (PEKC) by the third day. After the incubation with reovirus, the oncolytic activity was registered already in 24 hours: partial or complete destruction of the monolayer, decreased cell adhesion (only solitary cellular conglomerates remained at the bottom of vials), changes in the typical cell appearance. The complete cytopathogenic effect was observed after 2 days. Conclusions: The results demonstrated a marked lytic effect of reovirus in vitro, as well as varying sensitivity of the studied tumor lines to it, which may depend on their molecular genetic characteristics, for example on the RAS mutation status. Despite ambiguous results, we consider it expedient to continue the studies of these and other viruses in tumor models both in vitro and in vivo.

Sign in / Sign up

Export Citation Format

Share Document