scholarly journals Enhanced expression levels of aquaporin-1 and aquaporin-4 in A549 cells exposed to silicon dioxide

2016 ◽  
Vol 14 (3) ◽  
pp. 2101-2106 ◽  
Author(s):  
Xiaohui Hao ◽  
Hongli Wang ◽  
Wei Liu ◽  
Shupeng Liu ◽  
Zihe Peng ◽  
...  
Keyword(s):  
Silicon Dioxide ◽  
A549 Cells ◽  
Aquaporin 4 ◽  
Expression Levels ◽  
Aquaporin 1 ◽  
Enhanced Expression

Long-term regulation of four renal aquaporins in rats

1996 ◽  
Vol 271 (2) ◽  
pp. F414-F422 ◽  
Author(s):  
J. Terris ◽  
C. A. Ecelbarger ◽  
S. Nielsen ◽  
M. A. Knepper
Keyword(s):  
Aquaporin 4 ◽  
Osmotic Gradient ◽  
Brattleboro Rats ◽  
Sprague Dawley ◽  
Aquaporin 3 ◽  
Expression Levels ◽  
Aquaporin 1 ◽  
Aquaporin 2 ◽  
Sprague Dawley Rats

The aquaporins are molecular water channels expressed in the kidney and other organs. To investigate long-term regulation of renal expression of these water channels, we carried out immunoblotting studies using membrane fractions from rat renal cortex and medulla. Both 48-h water restriction in Sprague-Dawley rats and 5-day arginine vasopressin (AVP) infusion in Brattleboro rats caused significant increases in the expression levels of two aquaporins, aquaporin-2 and aquaporin-3, while the levels of aquaporin-1 and aquaporin-4 were unchanged. The increases in aquaporin-2 and aquaporin-3 expression were seen in inner and outer medulla as well as cortex. Ablation of the corticomedullary interstitial osmotic gradient with an infusion of furosemide did not eliminate the upregulatory response to AVP infusion in Brattleboro rats. Furthermore, 5-day furosemide infusion to Sprague-Dawley rats did not decrease expression levels of the collecting duct aquaporins, but rather increased them. We conclude that the expression of aquaporin-2 and aquaporin-3, but not aquaporin-1 or aquaporin-4, is increased in response to elevated circulating AVP. Because regulation of aquaporin-2 and aquaporin-3 levels was observed in the cortex and because osmotic gradient ablation did not abrogate the increase, we conclude that changes in interstitial osmolality are not necessary for the AVP-induced upregulation of aquaporin-2 and aquaporin-3 expression.

scholarly journals The time course changes in expression of aquaporin 4 and aquaporin 1 following global cerebral ischemic edema in rat

2016 ◽  
Vol 7 (1) ◽  
pp. 4 ◽  
Author(s):  
Gökhan Akdemir ◽  
Figen Kaymaz ◽  
Yasemin Gursoy-Özdemir ◽  
Nejat Akalan ◽  
EkinSu Akdemir
Keyword(s):  
Time Course ◽  
Aquaporin 4 ◽  
Aquaporin 1 ◽  
Course Changes ◽  
Cerebral Ischemic

Fetal development of membrane water channel proteins aquaporin‐1 and aquaporin‐4 in the human brain

2006 ◽  
Vol 24 (5) ◽  
pp. 295-305 ◽  
Author(s):  
Éva Gömöri ◽  
József Pál ◽  
Hajnalka Ábrahám ◽  
Zsolt Vajda ◽  
Endre Sulyok ◽  
...  
Keyword(s):  
Human Brain ◽  
Fetal Development ◽  
Water Channel ◽  
Aquaporin 4 ◽  
Aquaporin 1 ◽  
Channel Proteins

scholarly journals PDE4B Proposed as a High Myopia Susceptibility Gene in Chinese Population

2022 ◽  
Vol 12 ◽  
Author(s):  
Fuxin Zhao ◽  
Wei Chen ◽  
Hui Zhou ◽  
Peter S. Reinach ◽  
Yuhan Wang ◽  
...  
Keyword(s):  
Protein Expression ◽  
Normal Control ◽  
High Myopia ◽  
Susceptibility Gene ◽  
A549 Cells ◽  
Dna Pooling ◽  
Myopia Progression ◽  
Expression Levels ◽  
Replication Stage ◽  
Gene And Protein Expression

Myopia is the most common cause of refractive error worldwide. High myopia is a severe type of myopia, which usually accompanies pathological changes in the fundus. To identify high myopia susceptibility genes, DNA-pooling based genome-wide association analysis was used to search for a correlation between single nucleotide polymorphisms and high myopia in a Han Chinese cohort (cases vs. controls in discovery stage: 507 vs. 294; replication stage 1: 991 vs. 1,025; replication stage 2: 1,021 vs. 52,708). Three variants (rs10889602T/G, rs2193015T/C, rs9676191A/C) were identified as being significantly associated with high myopia in the discovery, and replication stage. rs10889602T/G is located at the third intron of phosphodiesterase 4B (PDE4B), whose functional assays were performed by comparing the effects of rs10889602T/T deletion of this risk allele on PDE4B and COL1A1 gene and protein expression levels in the rs10889602T/Tdel/del, rs10889602T/Tdel/wt, and normal control A549 cell lines. The declines in the PDE4B and COL1A1 gene expression levels were larger in the rs10889602T/T deleted A549 cells than in the normal control A549 cells (one-way ANOVA, p < 0.001). The knockdown of PDE4B by siRNA in human scleral fibroblasts led to downregulation of COL1A1. This correspondence between the declines in rs10889602 of the PDE4B gene, PDE4B knockdown, and COL1A1 protein expression levels suggest that PDE4B may be a novel high myopia susceptibility gene, which regulates myopia progression through controlling scleral collagen I expression levels. More studies are needed to determine if there is a correlation between PDE4B and high myopia in other larger sample sized cohorts.

scholarly journals PKN1 Is a Novel Regulator of Hippocampal GluA1 Levels

2021 ◽  
Vol 13 ◽  
Author(s):  
Motahareh Solina Safari ◽  
Dido Obexer ◽  
Gabriele Baier-Bitterlich ◽  
Stephanie zur Nedden
Keyword(s):  
Synaptic Proteins ◽  
The Novel ◽  
Serine Threonine Kinase ◽  
Expression Levels ◽  
Akt Phosphorylation ◽  
Protein Levels ◽  
Subunit Expression ◽  
Enhanced Expression ◽  
Synaptic Maturation ◽  
Specific Regulation

Alterations in the processes that control α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) expression, assembly and trafficking are closely linked to psychiatric and neurodegenerative disorders. We have recently shown that the serine/threonine kinase Protein kinase N1 (PKN1) is a developmentally active regulator of cerebellar synaptic maturation by inhibiting AKT and the neurogenic transcription factor neurogenic differentiation factor-2 (NeuroD2). NeuroD2 is involved in glutamatergic synaptic maturation by regulating expression levels of various synaptic proteins. Here we aimed to study the effect of Pkn1 knockout on AKT phosphorylation and NeuroD2 levels in the hippocampus and the subsequent expression levels of the NeuroD2 targets and AMPAR subunits: glutamate receptor 1 (GluA1) and GluA2/3. We show that PKN1 is expressed throughout the hippocampus. Interestingly, not only postnatal but also adult hippocampal phospho-AKT and NeuroD2 levels were significantly elevated upon Pkn1 knockout. Postnatal and adult Pkn1–/– hippocampi showed enhanced expression of the AMPAR subunit GluA1, particularly in area CA1. Surprisingly, GluA2/3 levels were not different between both genotypes. In addition to higher protein levels, we also found an enhanced GluA1 content in the membrane fraction of postnatal and adult Pkn1–/– animals, while GluA2/3 levels remained unchanged. This points toward a very specific regulation of GluA1 expression and/or trafficking by the novel PKN1-AKT-NeuroD2 axis. Considering the important role of GluA1 in hippocampal development as well as the pathophysiology of several disorders, ranging from Alzheimer’s, to depression and schizophrenia, our results validate PKN1 for future studies into neurological disorders related to altered AMPAR subunit expression in the hippocampus.

Mechanism of interaction between TM4SF1 and jak2-stat3 signaling pathway in lung cancer cells and expression analysis in non-small cell lung cancer

2020 ◽  
Author(s):  
Yumeng Niu ◽  
Hailong Deng ◽  
Lipeng Li ◽  
Weikang Chen ◽  
Yuxuan Wang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Signaling Pathway ◽  
A549 Cells ◽  
Normal Lung ◽  
Expression Levels ◽  
Qrt Pcr ◽  
Stat3 Signaling ◽  
Skin Cancers ◽  
Stat3 Signaling Pathway ◽  
Cancer Tissues

Abstract Background According to the latest data released in 2018, it is estimated that there will be 18.1 million new cancer cases worldwide (excluding 1.7 million non-melanoma skin cancers) and 9.6 million cancer deaths (excluding 950 non-melanoma skin cancers) Million cases). Among them, the incidence of lung cancer (11.6% of the total number of cases) and mortality (18.4% of the total number of cancer deaths, which are expected to cause 1.8 million deaths) are the first. In recent years, studies have found TM4SF1 play an important role in the development process of many tumors.Methods Sixty-one patients with NSCLC who underwent surgical resection of cancer tissues, para-carcinoma tissues, and 10 normal lung tissues removed from benign lung disease (Jun/2018-Dec/2018) were collected. Real-time immunofluorescence quantitative PCR (qRT-PCR) and Western blot were used to detect the expression of TM4SF1 in NSCLC tissues (CT), para-carcinoma tissue (PCT), and normal lung tissues(NLT). TM4SF1 gene was overexpressed in lung cancer A549 cells using lentiviral transfection technology, qRT-PCR and Western blot were used to detect whether TM4SF1 gene was successfully expressed in lung cancer A549 cells, and Transwell was used to detect the effect of TM4SF1 overexpression on A549 migration. JAK2-STAT3 signal pathway interference reagent AG490 was used to analyze the expression levels of Stat3 and downstream Sox2 genes in the overexpression group, blank group, negative control group and their corresponding treatment groups TM4SF1, JAK2-STAT3 signal pathway using real-time qRT-PCR. Analyze the relevance of these three indicators at the same time.Results The expression levels of TM4SF1 mRNA and protein in cancer tissues were significantly higher than those in adjacent cancer tissues (P<0.05) and normal lung tissue specimens (P <0.05). The expression of TM4SF1 was not significantly associated with the age and sex of patients, but was associated with tumor size, degree of differentiation, lymph node metastasis, and clinical stage were related (P<0.05). TM4SF1 was successfully overexpressed in A549 cells. After overexpressing TM4SF1, the ability to migrate of A549 cells was significantly enhanced, and the expression levels of Stat3 and downstream Sox2 in the JAK2-STAT3 signaling pathway were up-regulated. The expression of TM4SF1, Stat3 and Sox2 at the mRNA level showed a positive correlation trend (P<0.01).Conclusion TM4SF1 is highly expressed in NSCLC, and its expression level is closely related to many clinical staging indicators. Overexpression of this gene can promote the migration of A549 cells and up-regulate the expression levels of Stat3 and downstream Sox2 in the JAK2-STAT3 signaling pathway. The expressions of TM4SF1, Stat3 and Sox2 were positively correlated in A549 cells. TM4SF1 may promote the occurrence, development and distant metastasis of NSCLC through this pathway. TM4SF1 may become a potential therapeutic target for NSCLC.

scholarly journals Expression of Aquaporin 1 and Aquaporin 4 in the Temporal Neocortex of Patients with Parkinson's Disease

2016 ◽  
Vol 27 (2) ◽  
pp. 160-168 ◽  
Author(s):  
Akihiko Hoshi ◽  
Ayako Tsunoda ◽  
Mari Tada ◽  
Masatoyo Nishizawa ◽  
Yoshikazu Ugawa ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Aquaporin 4 ◽  
Aquaporin 1 ◽  
Temporal Neocortex

scholarly journals Systemic Hypertension Effects on the Ciliary Body and Iris. An Immunofluorescence Study with Aquaporin 1, Aquaporin 4, and Na+, K+ ATPase in Hypertensive Rats

Cells ◽  
2018 ◽  
Vol 7 (11) ◽  
pp. 210 ◽  
Author(s):  
Ibrahim González-Marrero ◽  
Luis Hernández-Abad ◽  
Emilia Carmona-Calero ◽  
Leandro Castañeyra-Ruiz ◽  
José Abreu-Reyes ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Intraocular Pressure ◽  
Aqueous Humor ◽  
Ciliary Body ◽  
Aquaporin 4 ◽  
Systemic Hypertension ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Aquaporin 1 ◽  
Wistar Kyoto

Aquaporin 1 (AQP1) and aquaporin 4 (AQP4) have been identified in the eye as playing an essential role in the formation of the aqueous humor along with the Na+/K+ ATPase pump. Different authors have described the relationship between blood pressure, aqueous humor production, and intraocular pressure with different conclusions, with some authors supporting a positive correlation between blood pressure and intraocular pressure while others disagree. The aim of this work was to study the effect of high blood pressure on the proteins involved in the production of aqueous humor in the ciliary body (CB) and iris. For this purpose, we used the eyes of spontaneously hypertensive rats (SHR) and their control Wistar-Kyoto rats (WKY). Immunofluorescence was performed in different eye structures to analyze the effects of hypertension in the expression of AQP1, AQP4, and the Na+/K+ ATPase α1 and α2 subunits. The results showed an increase in AQP1 and Na+/K+ ATPase α1 and a decrease in AQP4 and Na+/K+ ATPase α2 in the CB of SHR, while an increase in AQP4 and no significant differences in AQP1 were found in the iris. Therefore, systemic hypertension produced changes in the proteins implicated in the movement of water in the CB and iris that could influence the production rate of aqueous humor, which would be affected depending on the duration of systemic hypertension.

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