scholarly journals Celastrol negatively regulates cell invasion and migration ability of human osteosarcoma via downregulation of the PI3K/Akt/NF-κB signaling pathway in vitro

2016 ◽  
Vol 12 (5) ◽  
pp. 3423-3428 ◽  
Author(s):  
Xiaolong Yu ◽  
Qiang Wang ◽  
Xin Zhou ◽  
Changlin Fu ◽  
Ming Cheng ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Cell Invasion ◽  
Migration Ability ◽  
Invasion And Migration ◽  
Human Osteosarcoma ◽  
And Migration

scholarly journals Quantifying the invasion and migration ability of cancer cells with a 3D matrigel drop invasion assay

2021 ◽  
Author(s):  
Merve Aslan ◽  
En-Chi Hsu ◽  
Shiqin Liu ◽  
Tanya Stoyanova
Keyword(s):  
Cancer Cell ◽  
Cell Invasion ◽  
Cancer Metastasis ◽  
Drug Effects ◽  
Invasion Assay ◽  
Cancer Cell Invasion ◽  
Migration Ability ◽  
Invasion And Migration ◽  
And Migration

Abstract Metastasis is the main cause of cancer associated morbidity which will account for approximately 600,000 deaths in the USA in 2021. Defining new mechanisms that drive cancer metastasis is vital for developing new therapeutic strategies and improving clinical outcomes for cancer patients. Herein, we describe a recently established three-dimensional Matrigel drop invasion assay to measure cancer cell invasion and migration capability in vitro. This assay is a versatile and simple tool to test the ability of cells to invade and migrate, test the functional role of genes of interest in cell invasion and migration, analyze the localization of the target proteins at the cell invasion edge in situ, and screen drug effects on cancer cell invasion and migration.

The Mechanism of Human Umbilical Mesenchymal Stem Cells (HUMSC) on Biological Behavior of Human Papillomavirus (HPV) Cells Through Restraining the Programmed Cell Death Protein 1/Programmed Cell Death Ligand 1 (PD-1/PD-L1) Signal Pathway

2022 ◽  
Vol 12 (2) ◽  
pp. 265-272
Author(s):  
Min Wang ◽  
Yanong Zhu ◽  
Tongmin Li ◽  
Chaofeng Xia
Keyword(s):  
Cell Death ◽  
Test Group ◽  
Signal Pathway ◽  
Biological Behavior ◽  
Control Group ◽  
Migration Ability ◽  
Blank Group ◽  
Invasion And Migration ◽  
And Migration

Biological behavior of HPV cell was observed by HUMSC through restraining PD-1/PD-L1 signal pathway. And HUMSC was adopted as target cell for the treatment on HPV. The rat HPV model was established and divided into three groups including blank group, control group and test group according to different reagents being injected into rats. Use HE staining method to observe the cancerous transformation of tumor tissue sections. The gene presentation of PD-1/PD-L1 and lymphocyte was detected with Western blot. The invasion and migration condition of cancer cells was observed from experiment in vitro. The quantity of cancer cells in test group was the least. And invasion and migration ability in test group was the weakest. The control group was the second. The number of tumor cells in the blank group was the largest. Strongest ability to invade and migrate. The presentation of PD-L1 was restrained partly by HUMSC. The increasing of immune-associated cells could be prompted by HUMSC. The quantity of CD3+, CD4+ and CD8+ in PB was the most in test group. The expression of blank groups is the lowest than others restrained by HUMSC. And quantity of abundant immune cells including CD3+, CD4+ and CD8+ could be activated partly through activating immune action of body. And monitoring function of immune system on HPV cells could be increased effectively. The invasion and migration ability in vitro of HPV could be reduced partly.

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