scholarly journals Magnetic stent hyperthermia for esophageal cancer: An in vitro investigation in the ECA-109 cell line

2011 ◽  
Author(s):  
Ling-Yun Zhao
Keyword(s):  
Esophageal Cancer ◽  
Cell Line ◽  
In Vitro Investigation

scholarly journals Suppressive Effect of Fig (Ficus Carica) Latex on Esophageal Cancer Cell Proliferation

2013 ◽  
Vol 30 (2) ◽  
pp. 93-96 ◽  
Author(s):  
Seyyed Abbas Hashemi ◽  
Saeid Abediankenari
Keyword(s):  
Esophageal Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Human Cancer ◽  
Cancer Cell Line ◽  
Ficus Carica ◽  
Esophageal Cancer Cell ◽  
Fig Tree ◽  
Vitro Effect

SUMMARY Fig (Ficus carica) tree latex was a source of treatment of different diseases in the Iranian traditional medicine reported by Avicenna in his 10th century book Canon of Medicine. The aim of this investigation was to establish the anticancer effect of fig tree latex on human cancer cells. The in vitro effect of different doses of fig tree latex including 2.5 mg/ml, 5 mg/ml, and 10 mg/ml on esophageal cancer cell line was evaluated after 72 hours by MTT assay. There was a significant change in 10 mg/ml treatment of latex after 72 hours on esophageal cancer line (P; 0.025). Ten mg/ml was the optimum concentration in the inhibition of cell line growth. Fig (Ficus carica) tree latex could be a candidate as a potential agent for the inhibition of cancerous cells production and development.

In vitro Cytotoxicity of Bisphenol A in Monocytes Cell Line

2015 ◽  
Vol 40 (2) ◽  
pp. 180-186 ◽  
Author(s):  
Mauro Neri ◽  
Grazia Maria Virzì ◽  
Alessandra Brocca ◽  
Francesco Garzotto ◽  
Jeong Chul Kim ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Cell Line ◽  
Direct Contact ◽  
Cytotoxic Effect ◽  
In Vitro Cytotoxicity ◽  
Maximum Value ◽  
In Vitro Investigation ◽  
Apoptotic Effect ◽  
Monocyte Cell

Background/Aim: Bisphenol A (BPA) is used in the production of many plastics, which are used to build biomaterials that sometimes are in direct contact with blood. It is believed that the release of BPA into bloodstream may give rise to cytotoxic events for blood components. The aim of the present study was to perform an in vitro investigation of the observable cytotoxic effect of BPA, at increasing concentrations, on the monocyte cell line. Methods: We incubated in vitro monocyte cells (U937) for 24 h in cell line medium samples (RPMI 1640) at different concentrations of BPA. We then generated curves to evaluate viability, necrosis and apoptosis of monocytes against increasing concentrations of BPA. Results: The percentage values of concentrations of BPA corresponding to 50% of the viability and necrosis of the monocytes were 1.39 and 1.48 ng/ml, respectively. Based on our observations, we reported an increasing cytotoxic effect for higher concentrations. The apoptotic effect reached the maximum value at BPA concentration of 1.5 ng/ml; at still higher concentrations, we observed a predominantly necrotic cell death. Conclusion: Viability, necrosis and apoptosis of monocytes are strongly and positively correlated with BPA concentration. A direct contact of such compound with biological components of blood may lead to high levels of cytotoxicity, and require us to evaluate additional factors while judging the bio-incompatibility of BPA.

GLP-1(28–36) improves β-cell mass and glucose disposal in streptozotocin-induced diabetic mice and activates cAMP/PKA/β-catenin signaling in β-cells in vitro

2013 ◽  
Vol 304 (12) ◽  
pp. E1263-E1272 ◽  
Author(s):  
Weijuan Shao ◽  
Zhaoxia Wang ◽  
Wilfred Ip ◽  
Yu-Ting Chiang ◽  
Xiaoquan Xiong ◽  
...  
Keyword(s):  
Cyclin D1 ◽  
Cell Line ◽  
Cell Mass ◽  
Glucose Disposal ◽  
Β Cells ◽  
Β Cell ◽  
Pancreatic Β Cells ◽  
In Vitro Investigation

Recent studies have demonstrated that the COOH-terminal fragment of the incretin hormone glucagon-like peptide-1 (GLP-1), a nonapeptide GLP-1(28–36)amide, attenuates diabetes and hepatic steatosis in diet-induced obese mice. However, the effect of this nonapeptide in pancreatic β-cells remains largely unknown. Here, we show that in a streptozotocin-induced mouse diabetes model, GLP-1(28–36)amide improved glucose disposal and increased pancreatic β-cell mass and β-cell proliferation. An in vitro investigation revealed that GLP-1(28–36)amide stimulates β-catenin (β-cat) Ser675 phosphorylation in both the clonal INS-1 cell line and rat primary pancreatic islet cells. In INS-1 cells, the stimulation was accompanied by increased nuclear β-cat content. GLP-1(28–36)amide was also shown to increase cellular cAMP levels, PKA enzymatic activity, and cAMP response element-binding protein (CREB) and cyclic AMP-dependent transcription factor-1 (ATF-1) phosphorylation. Furthermore, GLP-1(28–36)amide treatment enhanced islet insulin secretion and increased the growth of INS-1 cells, which was associated with increased cyclin D1 expression. Finally, PKA inhibition attenuated the effect of GLP-1(28–36)amide on β-cat Ser675 phosphorylation and cyclin D1 expression in the INS-1 cell line. We have thus revealed the beneficial effect of GLP-1(28–36)amide in pancreatic β-cells in vitro and in vivo. Our observations suggest that GLP-1(28–36)amide may exert its effect through the PKA/β-catenin signaling pathway.

Chemical Composition Analysis and In Vitro Investigation of Cytotoxic and Antioxidative Activities of Iranian Propolis against Breast Cancer Cell Line, MCF-7

2018 ◽  
Vol 3 (39) ◽  
pp. 10857-10863 ◽  
Author(s):  
Fariba Asgharpour ◽  
Ali Akbar Moghadamnia ◽  
Sohrab Kazemi ◽  
Hamid Reza Nouri ◽  
Mahdi Pouramir ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Chemical Composition ◽  
Cell Line ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
Composition Analysis ◽  
In Vitro Investigation ◽  
Chemical Composition Analysis ◽  
Mcf 7

scholarly journals Phenanthrenes from Juncus Compressus Jacq. with Promising Antiproliferative and Anti-HSV-2 Activities

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 2085 ◽  
Author(s):  
Csaba Bús ◽  
Norbert Kúsz ◽  
Gusztáv Jakab ◽  
Seyyed Senobar Tahaei ◽  
István Zupkó ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Tumor ◽  
Tumor Cell Line ◽  
2D Nmr ◽  
Biologically Active ◽  
Active Components ◽  
In Vitro Investigation ◽  
First Time

Juncaceae species are rich sources of phenanthrenes. The present study has focused on the isolation and structure determination of biologically active components from Juncus compressus. Eleven compounds (nine phenanthrenes and two flavonoids) have been isolated from the plant by the combination of different chromatographic methods. Two compounds (compressins A (Compound 1) and B (Compound 2)) are novel natural products, while seven phenanthrenes (effusol (Compound 3), effususol (Compound 4), juncusol (Compound 5), 2-hydroxy-1-methyl-4-oxymethylene-5-vinyl-9,10-dihydrophenanthrene (Compound 6), 7-hydroxy-1-methyl-2-methoxy-5-vinyl-9,10-dihydrophenanthrene (Compound 7), effususin A (Compound 8), and dehydroeffusol (Compound 9)), and two flavonoids (apigenin (Compound 10) and luteolin (Compound 11) were isolated for the first time from the plant. Compressin B (Compound 2) is a dimeric phenanthrene, in which two juncusol monomers (Compound 5) are connecting through their C-3 atoms. The structure elucidation of the isolated compounds was carried out using 1D, 2D NMR spectroscopic methods and HR-MS measurements. In vitro investigation of the antiproliferative effect of the phenanthrenes on two cervical (HeLa and SiHa) and an ovarian human tumor cell line (A2780) revealed that compounds have remarkable antiproliferative activity, mainly on the HeLa cell line. Moreover, juncusol (Compound 5) proved to possess significant antiviral activity against the herpes simplex 2 virus (HSV-2).

Ultrastructure of Choriocarcinoma in Vitro

1969 ◽  
Vol 27 ◽  
pp. 362-363
Author(s):  
John C. Garancis ◽  
R. A. Pattillo
Keyword(s):  
Cell Line ◽  
Physiological Function ◽  
Cell System ◽  
Bewo Cell ◽  
Bewo Cells ◽  
Gestational Choriocarcinoma ◽  
Bewo Cell Line

Growth of cell system (BeWo-cell line) derived from human gestational choriocarcinoma has been established and continuously maintained in-vitro. Furthermore, it is evident from the previous studies that this cell line has retained the physiological function of the placental trophoblasts, namely the synthesis of human chorionic gonadotrophil(HCG).The BeWo cells were relatively small and possessed single nuclei, thus indicating that this cell line consists exclusively of cytotrophoblasts. In some instances cells appeared widely separated and their lateral surfaces were provided with numerous microvilli (Fig.1).

In vitro investigation of Varizella zoster virus as an oncolytic virus in glioblastoma therapy

2008 ◽  
Vol 35 (S 01) ◽  
Author(s):  
H Leske ◽  
A Baiker ◽  
C Schichor ◽  
J.C Tonn ◽  
R Goldbrunner ◽  
...  
Keyword(s):  
Oncolytic Virus ◽  
In Vitro Investigation ◽  
Varizella Zoster Virus
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