Wing geometry as a tool for studying the population structure of dwarf honey bees (Apis florea Fabricius 1876) in Iran

2009 ◽  
Vol 48 (4) ◽  
pp. 238-246 ◽  
Author(s):  
İrfan Kandemir ◽  
Mohammad G. Moradi ◽  
Berna Özden ◽  
Ayça Özkan
Keyword(s):  
Population Structure ◽  
Honey Bees ◽  
Apis Florea ◽  
Wing Geometry

scholarly journals Comparative nestmate recognition in Asian honey bees,Apis florea,Apis andreniformis,Apis dorsata, andApis cerana

Apidologie ◽  
2007 ◽  
Vol 38 (5) ◽  
pp. 411-418 ◽  
Author(s):  
Michael D. Breed ◽  
Xiao-Bao Deng ◽  
Robert Buchwald
Keyword(s):  
Honey Bees ◽  
Nestmate Recognition ◽  
Apis Florea ◽  
Apis Dorsata ◽  
Apis Andreniformis

scholarly journals Genetic diversity and population structure of two subspecies of western honey bees (Apis mellifera L.) in the Republic of South Africa as revealed by microsatellite genotyping

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8280
Author(s):  
Amin Eimanifar ◽  
Johanna T. Pieplow ◽  
Alireza Asem ◽  
James D. Ellis
Keyword(s):  
South Africa ◽  
Genetic Diversity ◽  
Population Structure ◽  
Apis Mellifera ◽  
Honey Bees ◽  
Molecular Ecology ◽  
Apis Mellifera Capensis ◽  
Nucleotide Polymorphisms ◽  
Invasive Pests ◽  
The Republic

Apis mellifera scutellata and Apis mellifera capensis, two native subspecies of western honey bees in the Republic of South Africa (RSA), are important to beekeepers in their native region because beekeepers use these bees for honey production and pollination purposes. Additionally, both bees are important invasive pests outside of their native ranges. Recently, whole mitogenome sequencing and single nucleotide polymorphisms were used to study their genetic diversity. To add to our knowledge of the molecular ecology of both bees, we tested the ability of microsatellites to be used as a tool to discriminate between A.m. capensis and A.m. scutellata. We analyzed the genetic variability and overall population structure of both bee subspecies and hybrids of the two by genotyping individuals collected from RSA (N = 813 bees from 75 apiaries) at 19 microsatellite DNA loci. Overall, populations averaged between 9.2 and 11.3 alleles per locus, with unbiased heterozygosity values ranging from 0.81 to 0.86 per population. Bayesian clustering analyses revealed two distinct evolutionary units, though the results did not match those of earlier morphometric and molecular analyses. This suggests that the microsatellites we tested were not sufficient for subspecies identification purposes, especially for Cape and hybrid bees. Nevertheless, the microsatellite data highlight the considerable genetic diversity within both populations and a larger-than-expected hybridization zone between the natural distributions of A.m. capensis and A.m. scutellata.

scholarly journals GENETIC DIVERSITY OF HONEY BEE POPULATIONS IN TURKEY BASED ON MICROSATELLITE MARKERS: A COMPARISON BETWEEN MIGRATORY VERSUS STATIONARY APIARIES AND ISOLATED REGIONS VERSUS REGIONS OPEN TO MIGRATORY BEEKEEPING

2019 ◽  
Author(s):  
Mert Kükrer
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Microsatellite Markers ◽  
Honey Bee ◽  
Honey Bees ◽  
Phylogenetic Trees ◽  
The Status ◽  
Genetic Impact ◽  
Geographic Populations ◽  
Ecological Importance

The honey bee (Apis mellifera L.) is a globally significant species of apparent economic and ecological importance. Recent reports from Spain, Italy and Greece point to an intense admixture of honey bee populations signified by a loss of population structure. This is mostly attributed to migratory beekeeping practices and replacement of queens or colonies with commercial ones that are usually from non-native races or hybrids of different subspecies. These two practices are also heavily carried out in parts of Turkey where almost three-quarters of the 6 million colonies are transferred seasonally from one region to other.Past research using microsatellite and RAPD markers, mtDNA, allozymes and geometric morphometry revealed the presence of five different subspecies of honey bees (meda, syriaca,caucasica, anatoliaca and an ecotype from Carniolan subspecies group) inTurkey. Here, we carried out an analysis of population structure of Turkish honeybees sampled from six different regions (n = 250) during the period 2010-2012. A total of 29 microsatellite markers were used in four multiplex reactions. The results show that population structure is preserved in general although there are signs of gene flow between the clusters.Overall FST between stationary colonies was calculated as 0,067. For migratory colonies the value was 0,015 and for all the 250 samples the value was 0,047. Four different clusters corresponding to geographical distributions of four subspecies were revealed in structure analysis. The differentiation between the clusters was also apparent in PCA and FCA as well as phylogenetic trees constructed based on genetic distances.The genetic impact of migratory beekeeping was demonstrated for the first time based on a comparison of assignment probabilities of individuals from migratory and stationary colonies to their geographic populations. Another comparison between regions that are either open to migratory beekeeping or closed let us to evaluate the status of isolated regions and showed the importance of establishing such regions. The effects of queen and colony trade were revealed by the presence of introgression from the highly commercial Caucasian bees. Our findings confirm the previously observed high levels geographically structured genetic diversity in honey bees of Turkey and emphasize the need to develop policies to maintain this diversity.

scholarly journals Honey Bee Diversity is Swayed by Migratory Beekeeping and Trade Despite Conservation Practices: Genetic Evidences for the Impact of Anthropogenic Factors on Population Structure

2019 ◽  
Author(s):  
Mert Kükrer ◽  
Meral Kence ◽  
Aykut Kence
Keyword(s):  
Population Structure ◽  
Gene Flow ◽  
Honey Bee ◽  
Honey Bees ◽  
Anthropogenic Impacts ◽  
Anthropogenic Factors ◽  
Conservation Implications ◽  
The Status ◽  
Bee Diversity ◽  
The Impact

Intense admixture of honey bee (Apis mellifera L.) populations is mostly attributed to migratory beekeeping practices and replacement of queens and colonies with non-native races or hybrids of different subspecies. These two practices are also heavily carried out in Anatolia and Thrace where 5 subspecies reside naturally.Here, we carried out an analysis of population structure of honey bees sampled from six different regions (n = 250) in order to test the genetic impacts of migratory beekeeping, queen and colony trade and conservation efficacy of isolated regions. A total of 30 microsatellite markers were used in four multiplex reactions.Direct genetic impact of migratory beekeeping was demonstrated first time based on a comparison of assignment of individuals to their geographically native populations where migratory colonies showed less fidelity. We found genetic evidence for them acting as a hybrid zone mobile in space and time, becoming vectors of otherwise local gene combinations.The effects of honey bee trade were revealed by the presence of very high introgression levels from the highly commercial Caucasian bees naturally limited to a narrow range. We also measured the direction and magnitude of this gene flow connected with bee trade.Comparison between regions that are either open to migratory beekeeping or not let us evaluate the status of isolated regions as centers of limited gene flow and showed the importance of establishing such regions.Despite signs of gene flow, our findings confirm high levels of geographically structured genetic diversity of four subspecies of honey bees in Turkey and emphasize the need to develop policies to maintain this diversity.Our overall results might potentially bear a wider interest to the community since they constitute an important attempt to quantify the effects of anthropogenic impacts on established patterns of honey bee diversity. Our measurable and justified findings on migratory beekeeping, queen and colony replacements as well as conservation implications will hopefully be of use for the decision makers and other stakeholders.

scholarly journals Scanning electron microscopic studies on tongue of open-nesting honey bees Apis dorsata F. and Apis florea F. (Hymenoptera: Apidae)

2015 ◽  
Vol 7 (1) ◽  
pp. 324-327
Author(s):  
Neelima R. Kumar ◽  
Kalpna Nayyar ◽  
Ruchi Sharma ◽  
Anudeep Anudeep
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Honey Bee ◽  
Honey Bees ◽  
Floral Resources ◽  
Apis Florea ◽  
Apis Dorsata ◽  
Electron Microscopic ◽  
Native Flora ◽  
Scanning Electron

Taste stimuli play vital role in the life of honey bees. Sensory structures observed on tongue of the honey bees with the help of Scanning electron microscopy (SEM) have become an important tool in analyzing honey bee biodiversity which offers an advanced diagnostic tool to study honey bee biogeography and determine adaptive variations to native flora. Tongue of honey bees present a high geographic variability in regard to the floral resources visited by the bees. The present study has determined to determine differences in the tongue ofopen-nesting bees by scanning electron microscopy of Apis dorsata and Apis florea. The two bees showed distinct morphological variations with respect to the lapping and sucking apparatus. It was observed that the ridges on the proximal region exhibited rough surface on A.dorsata whereas spinous in case of A.florea. Moreover, the arrangement of hair in the middle part of the tongue also differed in the two species. The shape of flabellum differed in the two species reason being the influence of native flora. It was observed that the shape of flabellum was oval in A.dorsata whereas in A.florea it was triangular. These differences indicated for the role of native flora and honey bee biodiversity.

scholarly journals Gut Bacterial Inhabitants of Open Nested Honey Bee, Apis Florea

2021 ◽  
Author(s):  
D.N. Ganeshprasad ◽  
Kunal Jani ◽  
Yogesh S. Shouche ◽  
A H Sneharani
Keyword(s):  
16S Rrna ◽  
Honey Bee ◽  
Honey Bees ◽  
Indian Subcontinent ◽  
Apis Florea ◽  
Culturable Bacteria ◽  
Bacterial Phyla ◽  
Environment And Health ◽  
Culture Independent ◽  
Dominant Bacteria

Abstract Honey bees are complex social system, which are highly dynamic having close interactions with their surrounding environment. Gut microbiota of honey bees has a major role in interaction behavior with its environment and health. Apis florea is the primitive among all the honey bees and are indigenous to Indian subcontinent. The study reports the identification and analysis of bacteria in the gut of wild species of honey bee, Apis florea, by culture-based and culture-independent methods. Cultured bacteria were identified and characterized by MALDI-TOF MS and 16S rRNA sequencing. A comprehensive analysis and identification of non-culturable bacteria were performed by 16S rRNA amplicon next generation sequencing. The sequence analysis approach classified gut bacteria into 5 bacterial phyla, 8 families and 10 genera in major. The dominant bacterial taxa identified in Apis florea belonged to Prevotellaceae (52.1%), Enterobacteriaceae (42.7%) and Halobacteriaceae (1.3%). The dominant bacteria belonged to genera of Prevotella, Escherichia-Shigella, Natronomonas, Methylobacterium, Pantoea, Bifidobacterium, Enterobacter, Klebsiella, Lactobacillus and Nitrobacter belonging to phyla Bacteroidetes, Proteobacteria, Euryarchaeota, Actinobacteria, and Firmicutes. Many of these bacteria identified herewith are not reported for their occurrence in others species of Apis genus making this study of highly relevance with respect to bee microbiome.

Sign in / Sign up

Export Citation Format

Share Document