scholarly journals Serum Cytokine Profile of Unaffected First-degree Relatives of Patients with Rheumatoid Arthritis

2014 ◽  
Vol 41 (2) ◽  
pp. 280-285 ◽  
Author(s):  
Lillian Barra ◽  
Kelly Summers ◽  
David Bell ◽  
Ewa Cairns
Keyword(s):  
Rheumatoid Arthritis ◽  
Discriminant Analysis ◽  
Disease Onset ◽  
Study Groups ◽  
Cytokine Profile ◽  
Healthy Controls ◽  
First Degree Relatives ◽  
Inflammatory Protein ◽  
Multiplexed Immunoassay ◽  
Macrophage Inflammatory Protein

Objective.Various cytokines have been implicated in the pathogenesis of rheumatoid arthritis (RA) and it is known that elevations in multiple cytokines occur prior to disease onset. The objective of our study is to determine whether the cytokine profile in unaffected first-degree relatives (FDR) of patients with RA is distinct from healthy controls.Methods.The sera of patients with RA, their unaffected FDR, and healthy controls were measured for 27 cytokines using a luminex-based multiplexed immunoassay. Subjects were also tested for rheumatoid factor and 6 different anticitrullinated protein/peptide antibodies (ACPA). Discriminant analysis was performed to define the cytokine profile of the 3 study groups.Results.Fourteen patients with RA, 37 unaffected FDR, and 27 healthy controls were enrolled. All patients with RA and 49% of FDR were ACPA-positive. Patients with RA had elevated levels of inflammatory cytokines compared to FDR and controls. Lower interleukin 13 (IL-13) levels were independently associated with RA. IL-4 was significantly lower in FDR compared to controls. Using discriminant analysis based on the levels of all cytokines measured, RA, FDR, and healthy controls could be distinguished with 91% accuracy. ACPA-positive subjects had higher levels of IL-9, but lower levels of IL-12p70 and macrophage inflammatory protein-1β. No individual cytokine was associated with ACPA-positivity in FDR, but the entire cytokine profile accurately distinguished ACPA-positive from ACPA-negative FDR.Conclusion.The cytokine profiles of patients with RA, healthy controls, unaffected ACPA-positive FDR, and ACPA-negative FDR appear to be distinct.

scholarly journals Role of Macrophage Inflammatory Protein-3α and Its Ligand CCR6 in Rheumatoid Arthritis

2003 ◽  
Vol 83 (4) ◽  
pp. 579-588 ◽  
Author(s):  
Jeffrey H Ruth ◽  
Shiva Shahrara ◽  
Christy C Park ◽  
Jacques C M Morel ◽  
Pawan Kumar ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Inflammatory Protein ◽  
Macrophage Inflammatory Protein

scholarly journals Macrophage inflammatory protein 1 alpha expression by synovial fluid neutrophils in rheumatoid arthritis

1999 ◽  
Vol 58 (5) ◽  
pp. 297-302 ◽  
Author(s):  
Y. Hatano ◽  
T. Kasama ◽  
H. Iwabuchi ◽  
R. Hanaoka ◽  
H. T Takeuchi ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Synovial Fluid ◽  
Inflammatory Protein ◽  
Macrophage Inflammatory Protein

scholarly journals Macrophage inflammatory protein-1 alpha. A novel chemotactic cytokine for macrophages in rheumatoid arthritis.

1994 ◽  
Vol 93 (3) ◽  
pp. 921-928 ◽  
Author(s):  
A E Koch ◽  
S L Kunkel ◽  
L A Harlow ◽  
D D Mazarakis ◽  
G K Haines ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Chemotactic Cytokine ◽  
Inflammatory Protein ◽  
Macrophage Inflammatory Protein

scholarly journals Dysregulated microRNA expression in rheumatoid arthritis families—a comparison between rheumatoid arthritis patients, their first-degree relatives, and healthy controls

2020 ◽  
Author(s):  
Emma Renman ◽  
Mikael Brink ◽  
Lisbeth Ärlestig ◽  
Solbritt Rantapää-Dahlqvist ◽  
Kristina Lejon
Keyword(s):  
Rheumatoid Arthritis ◽  
Microrna Expression ◽  
Significant Trend ◽  
Healthy Controls ◽  
Altered Expression ◽  
First Degree Relatives ◽  
Key Points ◽  
Polymerase Chain ◽  
And Gender

Abstract Objective Recent studies have demonstrated an altered expression of certain microRNAs in patients with rheumatoid arthritis (RA) as well as their first-degree relatives (FDRs) compared to healthy controls (HCs), suggesting a role of microRNA in the progression of the disease. To corroborate this, a set of well-characterized RA families originating from northern Sweden were analyzed for differential expression of a selected set of microRNAs. Method MicroRNA was isolated from frozen peripheral blood cells obtained from 21 different families and included 26 RA patients, 22 FDRs, and 21 HCs. Expression of the selected microRNAs miR-22-3p, miR-26b-5p, miR-34a-3p, miR-103a-3p, miR-142-3p, miR-146a-5p, miR-155, miR-346, and miR-451a was determined by a two-step quantitative real-time polymerase chain reaction (qRT-PCR). Statistical analysis including clinical variables was applied. Results Out of the nine selected microRNAs that previously have been linked to RA, we confirmed four after adjusting for age and gender, i.e., miR-22-3p (p = 0.020), miR-26b-5p (p = 0.018), miR-142-3p (p = 0.005), and miR-155 (p = 0.033). Moreover, a significant trend with an intermediate microRNA expression in FDR was observed for the same four microRNAs. In addition, analysis of the effect of corticosteroid use showed modulation of miR-103a-3p expression. Conclusions We confirm that microRNAs seem to be involved in the development of RA, and that the expression pattern in FDR is partly overlapping with RA patients. The contribution of single microRNAs in relation to the complex network including all microRNAs and other molecules is still to be revealed. Key Points• Expression levels of miR-22-3p, miR-26b-5p, miR-142-3p, and miR-155 were significantly altered in RA patients compared to those in controls.• In first-degree relatives, a significant trend with an intermediate microRNA expression in FDR was observed for the same four microRNAs.

The Viral Chemokine Macrophage Inflammatory Protein-II Is a Selective Th2 Chemoattractant

Blood ◽  
1998 ◽  
Vol 92 (11) ◽  
pp. 4036-4039 ◽  
Author(s):  
S. Sozzani ◽  
W. Luini ◽  
G. Bianchi ◽  
P. Allavena ◽  
T.N.C. Wells ◽  
...  
Keyword(s):  
T Cells ◽  
Chemokine Receptor ◽  
Cytokine Profile ◽  
Mononuclear Phagocytes ◽  
Th2 Cells ◽  
Type Ii ◽  
Cd8 Cells ◽  
T Helper 2 ◽  
Inflammatory Protein ◽  
Macrophage Inflammatory Protein

Abstract Kaposi’s sarcoma (KS) lesions are characterized by a prominent leukocyte infiltrate composed of mononuclear phagocytes and T cells. KS-associated CD4+ and CD8+ cells showed predominantly a type II cytokine profile. The CC chemokine viral macrophage inflammatory protein-II (vMIP-II) encoded by the KS-associated herpes virus 8 was a selective chemoattractant for T helper 2 (Th2 cells) and for monocytes, whereas it was inactive on other leukocytes, including Th1 cells, dendritic cells, and natural killer (NK) cells. vMIP-II was an agonist for CCR8, a chemokine receptor selectively expressed on CD4+ and CD8+ cells with a type II cytokine profile. Hence, vMIP-II has agonist activity for a chemokine receptor (CCR8), which is preferentially expressed on polarized Th2 cells. The capacity of vMIP-II to attract type II T cells selectively is likely to be a component of the virus strategy to subvert the host immune response.

scholarly journals Neurodevelopment regulators miR-137 and miR-34 family as biomarkers for early and adult onset schizophrenia

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Bao-Yu Chen ◽  
Jin-Jia Lin ◽  
Ming-Kun Lu ◽  
Hung-Pin Tan ◽  
Fong-Lin Jang ◽  
...  
Keyword(s):  
Operating Characteristic ◽  
Familial Aggregation ◽  
Differential Expression Analysis ◽  
Disease Onset ◽  
Healthy Controls ◽  
Adult Onset ◽  
Dual Roles ◽  
First Degree Relatives ◽  
Intermediate Phenotypes ◽  
Almost All

AbstractEarly-onset schizophrenia (EOS) may have stronger familial aggregation and a more severe outcome than adult-onset schizophrenia (AOS). MicroRNA (miRNA) takes on dual roles as a genetic and epigenetic modulator, which may mediate the influence of genetic risk. Neurological soft signs (NSS) are neurological abnormalities that may be intermediate phenotypes or endophenotypes for schizophrenia. Our previous study found poorer performance on NSS tests from patients with EOS and their unaffected first-degree relatives. Thus, we aimed to identify a set of aberrant neurodevelopmental-related miRNAs that could serve as potential biomarkers for EOS or schizophrenia with NSS. This study included 215 schizophrenia patients (104 EOS and 111 AOS), 72 unaffected first-degree relatives, 31 patients with bipolar disorder, and 100 healthy controls. Differential expression analysis revealed that miR-137, miR-34b, and miR-34c were significantly up-regulated in patients with schizophrenia and their unaffected first-degree relatives compared to healthy controls. Receiver operating characteristic (ROC) analysis showed that the miR-137 expression signature could be used to discriminate between patients with EOS and healthy controls (AUC = 0.911). Additionally, miR-34b had the highest ability to discriminate between EOS and AOS (AUC = 0.810), which may indicate different aetiological pathways to disease onset. Moreover, miR-137 dysregulation was correlated with almost all NSS subscales (i.e., sensory integration, motor sequencing, etc.) and, when EOS patients with NSS, miR-137 expression discriminated these patients from healthy controls to a greater extent (AUC = 0.957). These findings support the potential for neurodevelopmental-related miRNAs to be used as indicators of vulnerability to EOS.

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