scholarly journals Serum S100A12 and S100B Proteins are Independent Predictors of the Presence and Severity of Obstructive Sleep Apnea

2019 ◽  
Author(s):  
GÖZDE DEMİRCİ ◽  
ADİL ZAMANİ ◽  
ŞEBNEM YOSUNKAYA ◽  
İBRAHİM KILINÇ
Keyword(s):  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Linear Regression ◽  
Linear Regression Analysis ◽  
Treatment Compliance ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Apnea Hypopnea Index ◽  
Protein Levels ◽  
Obstructive Sleep

Background/aim: Obstructive sleep apnea (OSA) is associated with serious cardio-metabolic risks. Early diagnosis and treatment compliance are important. For this purpose, research is being carried out on biomarkers associated with the pathogenesis of the disease. We aimed to investigate whether serum S100A12 and S100B proteins could be used as biochemical markers in OSA patients to determine disease presence and severity. Materials and Methods: A total of 60 (16 women, 44 men) patients with OSA and 50 (20 women, 30 men) controls were enrolled in this cross-sectional study. Each subject included in the study underwent full-night polysomnography (PSG). The presence and severity of OSA was assessed by the apnea?hypopnea index (AHI). In the OSA group, 17-cases were mild, 18 were moderate, and 25 were severe. The serum levels of S100A12 and S100B were measured using the enzyme-linked immunosorbent assay (ELISA) technique. These protein levels were compared using the Student?s t-test in the patient and control groups. Spearman's rho correlation coefficients and corresponding p-values were calculated to determine the correlations between these protein levels and polysomnographic parameters. For evaluating the association between OSA and biomarkers, as well as possible confounding factors with S100A12 and S100B, we employed multiple linear regression analyses for the patients with OSA. Results: Serum levels of S100A12 and S100B were higher in patients than controls (p=0.01 and p=0.005, respectively), and a significant correlation was determined between S100A12 and S100B values and AHI (p=0.0001; p=0.0001), sleep time with SpO2< 90% (p=0.032; p=0.01), minimum SpO2 during sleep (p=0.019; p=0.007), and oxygen desaturation index ODI (p=0.001; p=0.0001). In the linear regression analysis, AHI was independently related with both S100A12 (p<?0.0001) and S100B (p=?0.011). Receiving operating curves (ROC) identified patients with OSA: AUC for S100A12=0.643; AUC for S100B=0.655 (p<0.05). Conclusion: Serum levels of S100B and S100A proteins have high diagnostic performance in OSA and are independent predictors of OSA presence and severity.

scholarly journals Prediction of optimal continuous positive airway pressure in Thai patients with obstructive sleep apnea

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Narongkorn Saiphoklang ◽  
Kanyada Leelasittikul ◽  
Apiwat Pugongchai
Keyword(s):  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Continuous Positive Airway Pressure ◽  
Airway Pressure ◽  
Positive Airway Pressure ◽  
Linear Regression Analysis ◽  
Prediction Equation ◽  
Apnea Hypopnea Index ◽  
Predictive Equation ◽  
Obstructive Sleep

AbstractContinuous positive airway pressure (CPAP) is simple and effective treatment for obstructive sleep apnea (OSA) patients. However, the CPAP prediction equation in each country is different. This study aimed to predict CPAP in Thai patients with OSA. A retrospective study was conducted in Thai patients, who OSA was confirmed by polysomnography and CPAP titration from January 2015 to December 2018. Demographics, body mass index (BMI), neck circumference (NC), Epworth sleepiness scale, apnea–hypopnea index (AHI), respiratory disturbance index (RDI), mean and lowest pulse oxygen saturation (SpO2), and optimal pressure were recorded. A total of 180 subjects were included: 72.8% men, age 48.7 ± 12.7 years, BMI 31.0 ± 6.3 kg/m2, NC 40.7 ± 4.1 cm, AHI 42.5 ± 33.0 per hour, RDI 47.1 ± 32.8 per hour, and lowest SpO2 77.1 ± 11.0%. Multiple linear regression analysis identified NC, BMI, RDI, and lowest SpO2. A final CPAP predictive equation was: optimal CPAP (cmH2O) = 4.614 + (0.173 × NC) + (0.067 × BMI) + (0.030 × RDI) − (0.076 × lowest SpO2). This model accounted for 50.0% of the variance in the optimal pressure (R2 = 0.50). In conclusion, a CPAP prediction equation can be used to explain a moderate proportion of the titrated CPAP in Thai patients with OSA. However, the CPAP predictive equation in each country may be different due to differences of ethnicity and physiology.Trial registration: TCTR20200108003.

scholarly journals 0170 Association of HIF-1 Alpha and Beta Subunits with Clock and BMAL1 Proteins in Obstructive Sleep Apnea Patients

SLEEP ◽  
2020 ◽  
Vol 43 (Supplement_1) ◽  
pp. A67-A67
Author(s):  
A Gabryelska ◽  
M Sochal ◽  
S Turkiewicz ◽  
P Bialasiewicz
Keyword(s):  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Circadian Clock ◽  
Hypoxia Inducible Factor ◽  
Study Groups ◽  
Oxygen Metabolism ◽  
Apnea Hypopnea Index ◽  
Protein Levels ◽  
Obstructive Sleep ◽  
Significant Difference

Abstract Introduction Obstructive sleep apnea (OSA) is a chronic condition that is characterized by intermittent hypoxia. Key regulator of oxygen metabolism is hypoxia inducible factor (HIF), which consists of oxygen sensitive subunit and continuously produced subunit. Circadian clock is composed of set of genes, which function as activators - CLOCK and BMAL 1, who similarly to HIF are basic helix-loop-helix-PER-ARNT-SIM transcription factors. Therefore, the aim of the study was to assess the relationship between HIF-1alpha, HIF-1beta, CLOCK, BMAL1 and polysomnography (PSG) variables in healthy individuals and severe OSA patients. Methods The study included 20 individuals, who underwent PSG and based on apnea-hypopnea index (AHI) were divided into severe OSA group (n=10; AHI30; 90% male) and healthy control (n=10; AHI&lt;5; 70% male). All participants had their peripheral blood collected in the evening (9:00-10:00 pm) before and in the morning (6:00-7:00 am) after the PSG. HIF-1alpha, HIF-1beta, CLOCK and BMAL1 protein concertation measurements were performed using ELISA. Results Significant difference was observed in the following protein measurements between study groups: evening and morning HIF-1 (p=0.020 and p=0.043, respectively), evening HIF-1alpha (p=0.047), evening and morning CLOCK (p=0.037 and p=0.019, respectively) and morning BMAL1 (p=0.016). No differences were observed between morning and evening protein levels in both groups. Evening HIF-1beta corraleted with evening CLOCK and morning BMAL1 (R=0.511, p=0.21 and R=0.594, p=0.006, respectively), while morning HIF-1 with evening BMAL1 (R=474, p=0.35). Furthermore, evening and morning HIF-1 correlated with evening BMAL1 (R=564, p=0.010 and R=0.689, p=0.001, respectively). Additionally, morning CLOCK and BMAL1 correlated with AHI (R=0.510, p=0.022 and R=0.560, p=0.010, respectively) and desaturation index (R=0.487, p=0.209 and R=0.570, p=0.009, respectively). Conclusion There is significant correlation between both subunits of HIF-1 protein and circadian clock proteins: CLOCK and BMAL1, which further correlate with increased disease severity. This suggests OSA patients are in risk of circadian clock disruption due to present hypoxia. Support The study was financed by Polish National Centre Grant no. 2018/31/N/NZ5/03931.

Physiological determinants of nocturnal arterial oxygenation in patients with obstructive sleep apnea

1985 ◽  
Vol 59 (5) ◽  
pp. 1364-1368 ◽  
Author(s):  
T. D. Bradley ◽  
D. Martinez ◽  
R. Rutherford ◽  
F. Lue ◽  
R. F. Grossman ◽  
...  
Keyword(s):  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Linear Regression Analysis ◽  
Gas Analysis ◽  
Apnea Hypopnea Index ◽  
Pulmonary Mechanics ◽  
Arterial Blood Gas ◽  
Arterial Blood ◽  
Nocturnal Hypoxemia ◽  
Obstructive Sleep

Among patients with similar degrees of obstructive sleep apnea (OSA) there is considerable variability in the degree of associated nocturnal hypoxemia. The factors responsible for this variability have not been clearly defined. Therefore we studied 44 patients with OSA to identify the physiological determinants of nocturnal arterial O2 saturation (SaO2). All patients underwent pulmonary function testing, arterial blood gas analysis, and overnight polysomnography. Mean nocturnal SaO2 ranged from 96 to 66% and apnea-hypopnea index from 11 to 128 per hour of sleep. Several anthropometric, respiratory physiological, and polysomnographic variables that could be expected to influence nocturnal SaO2 were entered into a stepwise multiple linear regression analysis, with mean nocturnal SaO2 as the dependent variable. Three variables [awake supine arterial PO2 (PaO2), expiratory reserve volume, and percentage of sleep time spent in apnea] were found to correlate strongly with mean nocturnal SaO2 (multiple R, 0.854; P less than 0.0001) and accounted for 73% of its variability among patients. Body weight, other lung volumes, and airflow rates influenced awake PaO2 and expiratory reserve volume but had no independent influence on nocturnal SaO2. In a further group of 15 patients with OSA a high correlation was obtained between measured nocturnal SaO2 and that predicted by the model (r = 0.87; P less than 0.001). We conclude that derangements of pulmonary mechanics and awake PaO2 (generally attributable to obesity and diffuse airway obstruction) are of major importance in establishing the severity of nocturnal hypoxemia in patients with OSA.

scholarly journals The Association Between Obstructive Sleep Apnea and Lung Nodule, Carcinoembryonic Antigen

2021 ◽  
Author(s):  
Ai-Ming Zeng ◽  
Li-Da Chen ◽  
Zhi-Wei Zhao ◽  
Meng-Xue Chen ◽  
Jie-Feng Huang ◽  
...  
Keyword(s):  
Obstructive Sleep Apnea ◽  
Regression Analysis ◽  
Sleep Apnea ◽  
Carcinoembryonic Antigen ◽  
Pulmonary Nodule ◽  
Linear Regression Analysis ◽  
Lung Nodule ◽  
Control Group ◽  
Apnea Hypopnea Index ◽  
Obstructive Sleep

Abstract Purpose: The association between obstructive sleep apnea (OSA) and cancer risks gaining more and more attention. Data on the association between OSA and lung cancer risk are limited. This study is to investigate whether a link exists between Low-dose computed tomography (LDCT) scanning of the chest findings, carcinoembryonic antigen (CEA) and OSA in patients suspected of OSA.Methods: The cross-sectional study included patients aged 18 years or older who underwent continuous nocturnal polysomnography at our sleep center between February 2019 and November 2020. All subjects underwent chest LDCT and CEA. Patients with an apnea-hypopnea index(AHI) of ≥15/h were classified as clinically significant OSA group, whereas patients with an AHI ＜15/h were classified as control group.Results: A total of 277 patients were enrolled in the study. 176 patients were categorized into the OSA group, while 101 patients were categorized into the control group. There is no relationship between any OSA-related parameter and presence of lung nodule or presence of ≥6mm lung nodule in the binary logistic regression analysis. OSA group demonstrated a significant higher value of CEA than control group. Stepwise multiple linear regression analysis showed that lowest O2 saturation(β=-0.256, p＜0.001), smoking status (β=0.156, p=0.007) and age (β=0.153, p=0.008) were independent predictors of elevated CEA. Conclusion: In a group of patients suspected of OSA, we confirmed an independent correlation between nocturnal hypoxia and elevated CEA levels. OSA was not related with presence of pulmonary nodule or≥6 mm pulmonary nodule in LDCT.

Alteration of Serum Levels of Inflammatory Cytokines and Polysomnographic Indices after Uvulopalatal Flap Surgery in Obstructive Sleep Apnea

2017 ◽  
Vol 96 (2) ◽  
pp. 65-68 ◽  
Author(s):  
Murad Mutlu ◽  
Erkan Vuralkan ◽  
Istemihan Akin ◽  
Hikmet Firat ◽  
Sadik Ardic ◽  
...  
Keyword(s):  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Cystatin C ◽  
Serum Levels ◽  
Intercellular Adhesion Molecule ◽  
Apnea Hypopnea Index ◽  
Intercellular Adhesion Molecule 1 ◽  
Obstructive Sleep ◽  
Tnf Α ◽  
Before And After

The aim of the current study was to compare the changes in polysomnographic indices and serum levels of C-reactive protein (CRP), cystatin C, tumor necrosis factor-α (TNF-α), and intercellular adhesion molecule-1 (ICAM-1) in patients with obstructive sleep apnea (OSA) who were treated surgically via a uvulopalatal flap (UPF) technique. Twenty-five patients (14 men, 11 women), average age 46.2 ± 9.3 years, who underwent UPF surgery were included in this study. Serum biochemical analyses and polysomnographic examinations were performed before and 6 months after the surgery. Pre- and postoperative values of apnea hypopnea index (AHI), oxygen desaturation index (ODI), and minimum oxygen concentrations, as well as serum levels of CRP, cystatin C, TNF-α, and ICAM-1 were compared. Comparison of variables before and after UPF surgery demonstrated that AHI (p = 0.001), ODI (p < 0.001) and oxygen saturation (p < 0.001) were significantly improved. In addition, serum levels of CRP (p = 0.036), cystatin C (p = 0.005), TNF-α (p < 0.001), and ICAM-1 (p < 0.001) were significantly reduced 6 months after surgery. Our results suggest that UPF is an effective surgical method that alleviates the severity of OSA. Moreover, it may have the potential to prevent the development of atherosclerosis by attenuating the inflammatory process induced by activation of inflammatory mediators such as CRP, TNF-α, ICAM-1, and cystatin C.

scholarly journals Irisin Is Correlated with Blood Pressure in Obstructive Sleep Apnea Patients

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Xing Wang ◽  
Zhengjiao Zhang ◽  
Xiaoxin Lan ◽  
Keyou Fu ◽  
Guanhua Xu ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Secondary Hypertension ◽  
The Other ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Apnea Hypopnea Index ◽  
Potential Biomarker ◽  
Obstructive Sleep

Background. Despite approximately 95% primary cases of hypertension, secondary hypertension seems to be common with resistant forms. Notably, obstructive sleep apnea (OSA) is known as a common cause of secondary hypertension and has a major characteristic of obesity. Irisin acts as a link between muscles and adipose tissues in obesity, playing an essential role in human blood pressure (BP) regulation. However, whether irisin is associated with secondary hypertension caused by OSA and how it takes effect essentially have not been elucidated. Purpose. To investigate the changes of irisin and its relationship with BP in OSA. Methods. 72 snoring patients finished Epworth Sleep Scale (ESS) evaluation before polysomnography (PSG). BP was the average of three brachial BP values by mercury sphygmomanometer. Serum irisin level was determined by enzyme-linked immunosorbent assay (ELISA). Results were analyzed by SPSS software. Results. Irisin was higher in the severe and quite severe group than that in control and nonsevere groups ( p < 0.05 ). For BP, significant differences were found between the control group and the other three groups ( p < 0.05 ) and between the quite severe and the other three groups ( p ≤ 0.001 ). Positive correlations were found between irisin and apnea-hypopnea index (AHI), AHI and BP, and irisin level and BP. Negative correlations were between irisin and SpO2 nadir and SpO2 nadir and BP. Positive correlation still existed between AHI and irisin even after adjusting for some obesity-related variables. Conclusions. Irisin may serve as a potential biomarker for severity of OSA independently of obesity and imply the development of hypertension.

scholarly journals Inflammatory Markers in Middle-Aged Obese Subjects: Does Obstructive Sleep Apnea Syndrome Play a Role?

2010 ◽  
Vol 2010 ◽  
pp. 1-6 ◽  
Author(s):  
Paschalis Steiropoulos ◽  
Nikolaos Papanas ◽  
Evangelia Nena ◽  
Maria Antoniadou ◽  
Evangelia Serasli ◽  
...  
Keyword(s):  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Obstructive Sleep Apnea Syndrome ◽  
Sleep Apnea Syndrome ◽  
Serum Levels ◽  
Blood Analysis ◽  
Apnea Hypopnea Index ◽  
Inflammation Markers ◽  
Obstructive Sleep ◽  
Apnea Syndrome

Background. Obstructive Sleep Apnea Syndrome (OSAS) is associated with inflammation, but obesity may be a confounding factor. Thus, the aim of this study was to explore differences in serum levels of inflammation markers between obese individuals with or without OSAS.Methods. Healthy individuals (n=61) from an outpatient obesity clinic were examined by polysomnography and blood analysis, for measurement of TNF-α, IL-6, CRP, and fibrinogen levels. According to Apnea-Hypopnea Index (AHI), participants were divided into two BMI-matched groups: controls (AHI < 15/h,n=23) and OSAS patients (AHI ≥ 15/h,n=38).Results. OSAS patients had significantly higher TNF-αlevels (P<.001) while no other difference in the examined inflammation markers was recorded between groups. Overall, TNF-αlevels were correlated with neck circumference (P<.001), AHI (P=.002), and Oxygen Desaturation Index (P=.002).Conclusions. Obese OSAS patients have elevated TNF-αlevels compared to BMI-matched controls, suggesting a role of OSAS in promoting inflammation, possibly mediated by TNF-a.

scholarly journals Friedman Score in Relation to Compliance and Treatment Response in Nonsevere Obstructive Sleep Apnea

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Lars M. Berg ◽  
Torun K. S. Ankjell ◽  
Yi-Qian Sun ◽  
Tordis A. Trovik ◽  
Anders Sjögren ◽  
...  
Keyword(s):  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Treatment Group ◽  
Treatment Compliance ◽  
Mandibular Advancement ◽  
Apnea Hypopnea Index ◽  
Mandibular Advancement Splint ◽  
Obstructive Sleep ◽  
Tongue Position ◽  
Sleep Recording

Nonsevere obstructive sleep apnea (OSA) is most often treated with a continuous positive airway pressure (CPAP) device or a mandibular advancement splint (MAS). However, patient compliance with these treatments is difficult to predict. Improvement in apnea-hypopnea index (AHI) is also somewhat unpredictable in MAS treatment. In this study, we investigated the association between Friedman tongue position score (Friedman score) and both treatment compliance and AHI improvement in patients with nonsevere OSA receiving CPAP or MAS treatment. 104 patients with nonsevere OSA were randomly allocated to CPAP or MAS treatment and followed for 12 months. Data were collected through a medical examination, questionnaires, sleep recordings from ambulatory type 3 polygraphic sleep recording devices, and CPAP recordings. Associations between Friedman score, treatment compliance, and AHI improvement were analysed with logistic regression analyses. Friedman score was not associated with treatment compliance (odds ratio [OR]: 0.85, 95% confidence interval [CI]: 0.59–1.23), or AHI improvement (OR: 1.05, 95% CI: 0.62–1.76) in the overall study sample, the CPAP treatment group, or the MAS treatment group. Adjustment for socioeconomic factors, body mass index, and tonsil size did not significantly impact the results. Although Friedman score may predict OSA severity and contribute to the prediction of success in uvulopalatopharyngoplasty, we found no association between Friedman score and treatment compliance in patients with nonsevere OSA receiving CPAP or MAS treatment, nor did we find any association between Friedman score and AHI improvement. Factors other than Friedman score should be considered when deciding whether a patient with nonsevere OSA should be treated with CPAP or MAS.

scholarly journals P060 Hypoxia-induced overexpression of Rev-Erb-alpha and NPAS2 proteins in obstructive sleep apnea patients - possible mechanism of DM2 development

2021 ◽  
Vol 2 (Supplement_1) ◽  
pp. A41-A41
Author(s):  
F Karuga ◽  
S Turkiewicz ◽  
M Ditmer ◽  
M Sochal ◽  
P Białasiewicz ◽  
...  
Keyword(s):  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Serum Protein ◽  
Protein Level ◽  
Control Group ◽  
Apnea Hypopnea Index ◽  
Orphan Nuclear Receptor ◽  
Protein Levels ◽  
Obstructive Sleep ◽  
Significant Difference

Abstract Circadian clocks are endogenous coordinators of 24-hour behavioral and molecular rhythms, which disruption may be caused by obstructive sleep apnea (OSA). It is composed of a set of genes, function as activators (CLOCK, BMAL) or repressors (PER, CRY). Neuronal PAS Domain Protein 2 (NPAS2) can substitute CLOCK in its function. Orphan nuclear receptor (Rev-Erb-α) is another protein supporting the CLOCK-BMAL1 complex, forming the loop which helps to regulate their expression. There are studies suggesting the significant influence of circadian disruption mediated via NPAS2 and Rev-Erb-α on DM2 development. The aim of the study was to determine the role of NPAS2 and Rev-Erb-α in DM2 for OSA patients. All participants underwent polysomnography (PSG) examination. Based on apnea-hypopnea index accompanied by clinical data the recruited individuals (n=40) were assigned to one from 3 groups: OSA (severe OSA, no DM2; n=17), DM2 (severe OSA + DM2; n=7) and control group (no OSA, no DM2; n=16). Serum protein levels of Rev-Erb-α and NPAS2 were assessed with ELISA immunoassay. Analysis between the groups revealed the statistically significant difference only in NPAS2 protein level (p=0.037). Further post-hoc analysis revealed significant differences between OSA and the control group (p=0.017). Moreover, a statistically significant correlation between AHI and NPAS2 serum protein level was observed (r=-0.478, p=0.002). NPAS2 protein levels are associated with a number of apneas and hypopneas during the REM phase of sleep and might have a significant role in the development of OSA complications. However, further studies are needed to understand its role.

Clinical Predictors of Pediatric Obstructive Sleep Apnea Syndrome

2018 ◽  
Vol 127 (9) ◽  
pp. 608-613 ◽  
Author(s):  
Chi-Chih Lai ◽  
Pei-Wen Lin ◽  
Hsin-Ching Lin ◽  
Michael Friedman ◽  
Hsueh-Wen Chang ◽  
...  
Keyword(s):  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Nasal Obstruction ◽  
Linear Regression Analysis ◽  
Mouth Breathing ◽  
Apnea Hypopnea Index ◽  
Physical Factors ◽  
Z Score ◽  
Adequate Model ◽  
Obstructive Sleep

Objective: To develop an adequate model using reliable clinical and physical factors to predict pediatric obstructive sleep apnea/hypopnea syndrome (OSAS). Methods: Complete anthropometric measurements including BMI z score, tonsil size grading, and updated Friedman tongue position (uFTP) were evaluated. Subjective assessments of clinical symptoms/signs, including snoring visual analog scale (VAS), nasal obstruction, and mouth breathing, were recorded. Results: Eighty-eight children (57 boys and 31 girls, mean age = 9.0 years) were confirmed to have OSAS by comprehensive polysomnography (PSG). When the aforementioned variables were analyzed individually, the results indicated that snoring VAS, nasal obstruction, mouth breathing, and BMI z score were reliable predictors of apnea/hypopnea index (AHI/h) values (correlation coefficient r = 0.386, P < .001; r = 0.416, P < .001; r = 0.255, P = .02; and r = 0.243, P = .02, respectively). When all significant factors were included in the stepwise multiple linear regression analysis, the final predictive model is: Pediatric AHI = 0.108 + 0.103 snoring VAS + 0.894 nasal obstruction + 0.207 BMI z score ( F = 4.06, P = .01). Conclusion: The proposed noninvasive, simple, inexpensive, and easy to perform screening tool could be used to predict pediatric OSAS. An abnormal calculated AHI may prompt clinical physicians to conduct further PSG diagnostics and treatment.

Sign in / Sign up

Export Citation Format

Share Document