Pediococcus acidilactici LAB4 and Lactobacillus plantarum LAB12 assimilate cholesterol and modulate ABCA1, CD36, NPC1L1 and SCARB1 in vitro

2017 ◽  
Vol 8 (1) ◽  
pp. 97-109 ◽  
Author(s):  
F.T. Lim ◽  
S.M. Lim ◽  
K. Ramasamy
Keyword(s):  
Lactobacillus Plantarum ◽  
Prolonged Exposure ◽  
Scavenger Receptor ◽  
Nile Red ◽  
Growth Media ◽  
Pediococcus Acidilactici ◽  
Down Regulation ◽  
Cholesterol Lowering ◽  
Ht29 Cells

There is growing interest in the use of probiotic lactic acid bacteria (LAB) for prevention of hypercholesterolaemia. This study assessed the cholesterol lowering ability of Pediococcus acidilactici LAB4 and Lactobacillus plantarum LAB12 in growth media. Both LAB yielded >98% (39.2 μg/ml) cholesterol lowering in growth media. Nile Red staining indicated direct assimilation of cholesterol by the LAB. The LAB were then explored for their prophylactic (pre-treatment of HT29 cells with LAB prior to cholesterol exposure) and biotherapeutic (treatment of HT29 cells with LAB after exposure to cholesterol) use against short and prolonged exposure of HT29 cells to cholesterol, respectively. For HT29 cells pre-treated with LAB, cholesterol lowering was accompanied by down-regulation of ATP-binding cassette family transporter-type A1 (ABCA1), cluster of differentiation 36 (CD36) and scavenger receptor class B member 1 (SCARB1). HT29 cells treated with LAB after prolonged exposure to cholesterol source, on the other hand, was associated with up-regulation of ABCA1, restoration of CD36 to basal level and down-regulation of Neimann-Pick C1-Like 1 (NPC1L1). The present findings implied the potential use of LAB4 and LAB12 as part of the strategies in prevention and management of hypercholesterolaemia.

scholarly journals Perfil fermentativo, estabilidade aeróbia e valor nutritivo de silagens de capim-marandu ensilado com aditivos

2008 ◽  
Vol 37 (10) ◽  
pp. 1728-1736 ◽  
Author(s):  
Thiago Fernandes Bernardes ◽  
Ricardo Andrade Reis ◽  
Rafael Camargo do Amaral ◽  
Gustavo Rezende Siqueira ◽  
Anna Paula de Toledo Piza Roth ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Pediococcus Acidilactici ◽  
Lactobacillus Buchneri

Com o objetivo de avaliar as perdas em silagem de capim-marandu produzidas com aditivos foram desenvolvidos dois experimentos. No experimento 1, objetivou-se conhecer o perfil de fermentação e a estabilidade aeróbia de quatro silagens: 1) forragem não tratada (Controle); 2) tratada com Lactobacillus plantarum e Propionibacterium; 3) tratada com Lactobacillus buchneri; e 4) tratada com 0,1% de benzoato de sódio. No experimento 2, foram utilizados nove novilhos castrados Nelore (PC de 350 ± 38,9 kg), alocados em três quadrados latinos 3 x 3 para avaliação do consumo e da digestibilidade das rações contendo 85,4% das seguintes silagens de capim-marandu: 1) controle; 2) controle com L. plantarum, Pediococcus acidilactici + enzimas fibrolíticas; e 3) tratamento 2 + L. buchneri. No experimento 1, as silagens apresentaram baixas recuperações de MS durante a fermentação (média de 86%) e os coeficientes de digestibilidade in vitro da matéria seca reduziram de 65,5% (momento da ensilagem) para 50,0% no 60º dia após o fechamento dos silos. No experimento 2, o valor médio de consumo das rações foi de 5,7 kg MS/dia (1,6% PC) e a digestibilidade de 51,6% e não diferiram entre as rações. As silagens apresentaram perdas acentuadas na fase fermentativa e o uso de aditivos não alterou essas perdas. A inoculação com bactérias não influenciou o consumo ou a digestibilidade das rações.

Cholesterol lowering by Pediococcus acidilactici LAB4 and Lactobacillus plantarum LAB12 in adult zebrafish is associated with improved memory and involves an interplay between npc1l1 and abca1

2017 ◽  
Vol 8 (8) ◽  
pp. 2817-2828 ◽  
Author(s):  
Fei Tieng Lim ◽  
Siong Meng Lim ◽  
Kalavathy Ramasamy
Keyword(s):  
Learning And Memory ◽  
Lactobacillus Plantarum ◽  
Spatial Learning ◽  
Spatial Learning And Memory ◽  
Pediococcus Acidilactici ◽  
Adult Zebrafish ◽  
Cholesterol Lowering

P. acidilacticiLAB4 andL. plantarumLAB12 lowered cholesterol in adult zebrafishviaintestinal/hepaticnpc1l1andabca1modulation as well as brainappa, which was correlated with improved spatial learning and memory.

scholarly journals Screening for Cholesterol-Lowering Probiotics from Lactic Acid Bacteria Isolated from Corn Silage Based on Three Hypothesized Pathways

2019 ◽  
Vol 20 (9) ◽  
pp. 2073 ◽  
Author(s):  
Changlu Ma ◽  
Shuwen Zhang ◽  
Jing Lu ◽  
Cai Zhang ◽  
Xiaoyang Pang ◽  
...  
Keyword(s):  
Bile Salt ◽  
Down Regulation ◽  
Cholesterol Removal ◽  
Cholesterol Lowering ◽  
Probiotic Potential ◽  
Cholesterol Levels ◽  
Bile Salt Deconjugation ◽  
Lowering Activity

A total of 85 strains of lactic acid bacteria were isolated from corn silage in this study and analyzed in vitro for their cholesterol removal, NPC1L1 protein down-regulation and bile salt deconjugation ability, respectively. Nineteen strains were selected for further analysis for their probiotic potential. Finally, 3 strains showing better probiotic potential were evaluated for their cholesterol-lowering activity in hamsters. The strains showing the greater cholesterol removal and NPC1L1 protein down-regulation activity had no significant effects on serum and hepatic cholesterol levels in hamsters (p > 0.05). However, Lactobacillus plantarum CAAS 18008 (1 × 109 CFU/d) showing the greater bile salt deconjugation ability significantly reduced serum low-density lipoprotein cholesterol, total cholesterol, and hepatic total cholesterol levels by 28.8%, 21.7%, and 30.9%, respectively (p < 0.05). The cholesterol-lowering mechanism was attributed to its bile salt hydrolase activity, which enhanced daily fecal bile acid excretion levels and thereby accelerated new bile acid synthesis from cholesterol in liver. This study demonstrated that the strains showing greater cholesterol removal and NPC1L1 protein down-regulation activity in vitro hardly reveal cholesterol-lowering activity in vivo, whereas the strains showing greater bile salt deconjugation ability in vitro has large potential to decrease serum cholesterol levels in vivo.

Use of Lactobacillus plantarum (strains 22F and 25F) and Pediococcus acidilactici (strain 72N) as replacements for antibiotic-growth promotants in pigs

2021 ◽  
Author(s):  
Pawiya Pupa ◽  
Prasert Apiwatsiri ◽  
Wandee Sirichokchatchawan ◽  
Noppadon Pirarat ◽  
Tanawong Maison ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Average Daily Gain ◽  
Intestinal Morphology ◽  
Pediococcus Acidilactici ◽  
Feed Conversion ◽  
Peptone Water ◽  
Antibiotic Group ◽  
Growth Promotants ◽  
Spray Dried

Abstract Lactobacillus plantarum (strains 22F and 25F) and Pediococcus acidilactici (strain 72N) have appeared promising in in vitro studies. Microencapsulation, especially by the spray-drying method, has been used to preserve their numbers and characteristics during storage and digestion. This study compared the efficacy of these LAB strains and their microencapsulated form with antibiotic usage on growth performance, faecal microbial counts, and intestinal morphology in nursing-finishing pigs. A total of 240 healthy neonatal pigs were treated on days 0, 3, 6, 9, and 12 after cross-fostering. Sterile peptone water was delivered orally to the control and antibiotic groups. Six-month storage spray-dried Lactobacillus plantarum strain 22F was administered to piglets in the spraydry group. The three fresh strains of LAB (109 CFU/mL) were orally administered to piglets in each group. All pigs received the basal diets, but these were supplemented with routine antibiotic for the antibiotic group. Pigs in the probiotic supplemented groups (spray-dried and three fresh strains of LAB) exhibited a better average daily gain and feed conversion ratio than those of the controls in the nursery and grower phases. Probiotic supplementation increased viable lactobacilli and decreased enterobacterial counts. Antibiotic additives reduced both enterobacterial and lactobacilli counts. Villous height and villous height:crypt depth ratio were greater in probiotic and antibiotic supplemented pigs comparing to the controls, especially in the jejunum. The results demonstrated the feasibility of using these strains as a substitute for antibiotics and the practicality of the microencapsulation protocol for use in swine farms.

scholarly journals Efeitos da adição de inoculantes microbianos sobre a composição bromatológica e perfil fermentativo da silagem de sorgo produzida em silos experimentais

2002 ◽  
Vol 31 (6) ◽  
pp. 2373-2379 ◽  
Author(s):  
Paulo Henrique Mazza Rodrigues ◽  
Ana Lúcia Senatore ◽  
Stefano Juliano Tavares de Andrade ◽  
Juliana Martins Ruzante ◽  
Carlos de Sousa Lucci ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Pediococcus Acidilactici ◽  
Streptococcus Faecium

Foram estudados os efeitos de três inoculantes microbianos, na silagem de sorgo (Agroceres 2005), em 16 silos experimentais, confeccionados a partir de baldes plásticos portando válvulas. O sorgo, cortado aos 97 dias de crescimento (35,9% de MS), foi homogeneizado e submetido a quatro tratamentos, com quatro repetições: controle, Sil-All (Streptococcus faecium, Pediococcus acidilactici, Lactobacillus plantarum, amilase, hemicelulase e celulase), Silobac (L. plantarum, S. faecium e Lactobacillus sp) e Pioneer 1174 (S. faecium e L. plantarum). Os silos foram abertos após 120 dias para análise da composição bromatológica e perfil fermentativo. Os três inoculantes diminuíram o teor de MS e aumentaram a concentração de etanol e as perdas de MS, em relação ao grupo controle. O Sil-All aumentou o teor de FDN, em relação ao grupo controle, e diminuiu a concentração de amido, em relação ao Silobac, enquanto que o Pioneer 1174 diminuiu a estabilidade aeróbia, em relação ao grupo controle. Não foram observados efeitos sobre os teores de PB, NIDA, carboidratos solúveis, FDA, lignina, hemicelulose, celulose, a digestibilidade in vitro da matéria seca, o poder tampão e as concentrações dos ácidos acético, propiônico, butírico e lático, bem como sobre o pH ou sobre as concentrações de nitrogênio amoniacal.

Down regulation of osteopontin interferes with HCV replication by inhibition of WNT signalling in vitro

2013 ◽  
Vol 51 (01) ◽  
Author(s):  
C Loscher ◽  
R Bartenschlager ◽  
V Lohmann ◽  
G Tiegs ◽  
G Sass
Keyword(s):  
Wnt Signalling ◽  
Down Regulation

Role of 4-O Galloylchlorogenic Acid in Lung Cancer- An Insilico Approach

2017 ◽  
Vol 9 (5) ◽  
Author(s):  
Jaynthy C. ◽  
N. Premjanu ◽  
Abhinav Srivastava
Keyword(s):  
Lung Cancer ◽  
Cell Proliferation ◽  
In Silico ◽  
Computational Study ◽  
Regulation Mechanism ◽  
Down Regulation ◽  
Fruit Extract ◽  
In Vitro Techniques ◽  
Discovery Studio

Cancer is a major disease with millions of patients diagnosed each year with high mortality around the world. Various studies are still going on to study the further mechanisms and pathways of the cancer cell proliferation. Fucosylation is one of the most important oligosaccharide modifications involved in cancer and inflammation. In cancer development increased core fucosylation by FUT8 play an important role in cell proliferation. Down regulation of FUT8 expression may help cure lung cancer. Therefore the computational study based on the down regulation mechanism of FUT8 was mechanised. Sapota fruit extract, containing 4-Ogalloylchlorogenic acid was used as the inhibitor against FUT-8 as target and docking was performed using in-silico tool, Accelrys Discovery Studio. There were several conformations of the docked result, and conformation 1 showed 80% dock score between the ligand and the target. Further the amino acids of the inhibitor involved in docking were studied using another tool, Ligplot. Thus, in-silico analysis based on drug designing parameters shows that the fruit extract can be studied further using in-vitro techniques to know its pharmacokinetics.

Methods of experimental polyploidization and their use in apple breeding

2020 ◽  
Vol 62 ◽  
pp. 85-90
Author(s):  
L. V. Tashmatova ◽  
O. V. Matsneva ◽  
T. M. Khromova ◽  
V. V. Shakhov
Keyword(s):  
Exposure Time ◽  
Cell Walls ◽  
Prolonged Exposure ◽  
Ornamental Plants ◽  
Colchicine Treatment ◽  
Apple Trees ◽  
Open Ground ◽  
High Concentrations ◽  
Diploid Gametes

The article presents methods of experimental polyploidy of fruit, berry and ornamental plants. The purpose of this review is to highlight the problems and prospects of polyploidization of plants in the open ground and in vitro culture and the possibility of their application for apple trees. For the purpose of obtaining apple tetraploids as donors of diploid gametes, seed seedlings were treated with a solution of colchicine in concentrations of 0.1-0.4 % for 24 and 48 hours. Colchicine concentrations of 0.3 % and 0.4 % at 48 hours of treatment had a detrimental eff ect on their development. As a result, tetraploids and chimeras were obtained from seeds from free pollination of the varieties Orlik, Svezhest, Kandil Orlovsky, as well as from seeds obtained from crossing the varieties Svezhest×Bolotovskoe, Moskovskoe Оzherel’e×Imrus, Girlyanda×Venyaminovskoe. The optimal concentration of colchicine was 0.1 %. Methods of colchicine treatment have been studied: 1) adding to the nutrient medium, colchicine concentration: 0.01%, 0.02%, exposure time 24h-19 days; 2) applying amitotic solution to the growth point, colchicine concentration: 0.1 %, 0.2 %, exposure time 24h-7 days. To increase the penetration of colchicine through the cell walls, a 0.1 % dimexide solution was used. Studies have shown that high concentrations and prolonged exposure to colchicine reduce the viability of explants.

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