Encapsulation of Lactobacillus plantarum ATCC 8014 and Pediococcus acidilactici ATCC 8042 in a freeze-dried alginate-gum arabic system and its in vitro testing under gastrointestinal conditions

Com o objetivo de avaliar as perdas em silagem de capim-marandu produzidas com aditivos foram desenvolvidos dois experimentos. No experimento 1, objetivou-se conhecer o perfil de fermentação e a estabilidade aeróbia de quatro silagens: 1) forragem não tratada (Controle); 2) tratada com Lactobacillus plantarum e Propionibacterium; 3) tratada com Lactobacillus buchneri; e 4) tratada com 0,1% de benzoato de sódio. No experimento 2, foram utilizados nove novilhos castrados Nelore (PC de 350 ± 38,9 kg), alocados em três quadrados latinos 3 x 3 para avaliação do consumo e da digestibilidade das rações contendo 85,4% das seguintes silagens de capim-marandu: 1) controle; 2) controle com L. plantarum, Pediococcus acidilactici + enzimas fibrolíticas; e 3) tratamento 2 + L. buchneri. No experimento 1, as silagens apresentaram baixas recuperações de MS durante a fermentação (média de 86%) e os coeficientes de digestibilidade in vitro da matéria seca reduziram de 65,5% (momento da ensilagem) para 50,0% no 60º dia após o fechamento dos silos. No experimento 2, o valor médio de consumo das rações foi de 5,7 kg MS/dia (1,6% PC) e a digestibilidade de 51,6% e não diferiram entre as rações. As silagens apresentaram perdas acentuadas na fase fermentativa e o uso de aditivos não alterou essas perdas. A inoculação com bactérias não influenciou o consumo ou a digestibilidade das rações.

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Viability of microencapsulated Akkermansia muciniphila and Lactobacillus plantarum during freeze-drying, storage and in vitro simulated upper gastrointestinal tract passage

Food & Function ◽

10.1039/c8fo01331d ◽

2018 ◽

Vol 9 (11) ◽

pp. 5868-5879 ◽

Cited By ~ 19

Author(s):

Martín Sebastián Marcial-Coba ◽

Tomasz Cieplak ◽

Thiago Barbosa Cahú ◽

Andreas Blennow ◽

Susanne Knøchel ◽

...

Keyword(s):

Gastrointestinal Tract ◽

Lactobacillus Plantarum ◽

Freeze Drying ◽

Storage Stability ◽

Upper Gastrointestinal Tract ◽

Akkermansia Muciniphila ◽

Freeze Dried ◽

Upper Gastrointestinal

Microencapsulated and subsequently freeze-dried cells showed acceptable storage stability and enhanced survival during in vitro upper gastrointestinal tract passage.

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Pediococcus acidilactici LAB4 and Lactobacillus plantarum LAB12 assimilate cholesterol and modulate ABCA1, CD36, NPC1L1 and SCARB1 in vitro

Beneficial Microbes ◽

10.3920/bm2016.0048 ◽

2017 ◽

Vol 8 (1) ◽

pp. 97-109 ◽

Cited By ~ 5

Author(s):

F.T. Lim ◽

S.M. Lim ◽

K. Ramasamy

Keyword(s):

Lactobacillus Plantarum ◽

Prolonged Exposure ◽

Scavenger Receptor ◽

Nile Red ◽

Growth Media ◽

Pediococcus Acidilactici ◽

Down Regulation ◽

Cholesterol Lowering ◽

Ht29 Cells

There is growing interest in the use of probiotic lactic acid bacteria (LAB) for prevention of hypercholesterolaemia. This study assessed the cholesterol lowering ability of Pediococcus acidilactici LAB4 and Lactobacillus plantarum LAB12 in growth media. Both LAB yielded >98% (39.2 μg/ml) cholesterol lowering in growth media. Nile Red staining indicated direct assimilation of cholesterol by the LAB. The LAB were then explored for their prophylactic (pre-treatment of HT29 cells with LAB prior to cholesterol exposure) and biotherapeutic (treatment of HT29 cells with LAB after exposure to cholesterol) use against short and prolonged exposure of HT29 cells to cholesterol, respectively. For HT29 cells pre-treated with LAB, cholesterol lowering was accompanied by down-regulation of ATP-binding cassette family transporter-type A1 (ABCA1), cluster of differentiation 36 (CD36) and scavenger receptor class B member 1 (SCARB1). HT29 cells treated with LAB after prolonged exposure to cholesterol source, on the other hand, was associated with up-regulation of ABCA1, restoration of CD36 to basal level and down-regulation of Neimann-Pick C1-Like 1 (NPC1L1). The present findings implied the potential use of LAB4 and LAB12 as part of the strategies in prevention and management of hypercholesterolaemia.

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Encapsulation of Lactobacillus acidophilus NCDC 016 cells by spray drying: characterization, survival after in vitro digestion, and storage stability

Food & Function ◽

10.1039/d0fo01394c ◽

2020 ◽

Vol 11 (10) ◽

pp. 8694-8706

Author(s):

Divyasree Arepally ◽

Ravula Sudharshan Reddy ◽

Tridib Kumar Goswami

Keyword(s):

Spray Drying ◽

Lactobacillus Acidophilus ◽

Storage Stability ◽

Gum Arabic ◽

In Vitro Digestion ◽

Encapsulated Cells ◽

Gastrointestinal Conditions ◽

Drying Technology ◽

And Storage

L. acidophilus was encapsulated with maltodextrin and different concentrations of gum arabic by spray drying technology. Encapsulated cells have shown better viability under simulated gastrointestinal conditions compared to free cells.

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Lactobacillus plantarum immobilized onto soymilk residue (Okara) for the enhancement of soymilk fermentation and cell survival under simulated gastrointestinal conditions

10.7287/peerj.preprints.2349 ◽

2016 ◽

Author(s):

Xia Xiudong ◽

Wang Ying ◽

Liu Xiaoli ◽

Li Ying ◽

Zhou Jianzhong

Keyword(s):

Lactobacillus Plantarum ◽

Specific Growth ◽

Cell Immobilization ◽

Low Ph ◽

Lag Phase ◽

Cell Resistance ◽

Food Grade ◽

Titrable Acidity ◽

Gastrointestinal Conditions

Cell immobilization is an alternative to microencapsulation for the maintenance of cells in a liquid medium. However, artificial immobilization carriers are expensive and pose a high safety risk. This study aimed to evaluate the potential of okara, a food-grade byproduct from soymilk production, as a natural immobilizer for L. plantarum 70810 cells. The study also aimed to evaluate the effects of okara-immobilized L. plantarum 70810 cells on soymilk fermentation, glucosidic isoflavone bioconversion, and cell resistance to gastrointestinal (GI) stress. Scanning electron microscopy revealed that the lactobacilli cells attached and bound to okara’s surface. Compared with the free cells (FL), immobilized Lactobacillus plantarum (IL) cells exhibited a significantly higher specific growth rate and shorter lag phase of growth, a faster decrease in pH and increase in titrable acidity, and a higher soymilk viscosity. Similarly, IL in soymilk showed higher productions of daizein and genistein compared with the control. Compared with FL, IL showed reinforced resistance to simulatedGI stress in vitro that included low pH, low pH plus pepsin, pancreatin, and bile salt. Our results indicate that okara is a new potential immobilization carrier to enhance the growth and glucosidic isoflavone bioconversion activities of L. plantarum in soymilk and improve cell survivability following GI transit.

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Effects of oligosaccharides on the growth and stress tolerance of Lactobacillus plantarum ZLP001 in vitro, and the potential synbiotic effects of L. plantarum ZLP001 and fructo-oligosaccharide in post-weaning piglets1

Journal of Animal Science ◽

10.1093/jas/skz254 ◽

2019 ◽

Vol 97 (11) ◽

pp. 4588-4597 ◽

Cited By ~ 2

Author(s):

Jing Wang ◽

Sixin Wang ◽

Hui Liu ◽

Dongyan Zhang ◽

Yamin Wang ◽

...

Keyword(s):

Stress Tolerance ◽

Lactobacillus Plantarum ◽

Combination Treatment ◽

Average Daily Gain ◽

Nutrient Digestibility ◽

Feed Consumption ◽

Freeze Dried ◽

Weaning Piglets ◽

G Ratio

Abstract In this study, we evaluated the effects of seven oligosaccharides on the growth rate and stress tolerance of Lactobacillus plantarum ZLP001 in vitro, and the potential synbiotic effects of the most effective oligosaccharide [fructo-oligosaccharide (FOS)] and L. plantarum ZLP001 on the growth performance, apparent nutrient digestibility, fecal microbiota, and serum immune index in weaning piglets. Most oligosaccharides were utilized as carbohydrate sources by L. plantarum ZLP001, but we observed obvious differences in the bacterial growth depending on oligosaccharide type and concentration. Oligosaccharides and glucose significantly alleviated the decrease in L. plantarum ZLP001 viability in artificial gastric fluid, whereas none of the sugars affected viability in artificial intestinal fluid. FOS and galacto-oligosaccharide significantly improved the viability of L. plantarum ZLP001 under heat stress (65 °C for 15 and 30 min). FOS and soybean oligosaccharide significantly increased the viability of L. plantarum ZLP001 in response to cold stress (4 °C for 30 and 60 days). On the basis of the findings of in vitro experiments, we selected FOS for in vivo studies. Eighty-four weaned piglets were randomly assigned to one of the following groups: control (basal diet, no additives), freeze-dried L. plantarum ZLP001 (4.2 × 109 CFU/g, 2 g/kg diet), FOS (5 g/kg diet), and combination (0.2% L. plantarum ZLP001 + 0.5% FOS). Body weight and feed consumption were recorded for determinations of the average daily gain (ADG), average daily feed intake (ADFI), and feed-to-gain ratio (F/G). On day 28, fresh fecal samples were collected to evaluate the apparent digestibility of nutrients and microbiota, and serum samples were collected to determine the immune status. L. plantarum ZLP001 plus FOS significantly increased ADG and decreased the F/G ratio compared with the no-additive control. The combination treatment also increased the apparent nutrient digestibility of dry matter and crude protein. Compared with the control and single supplementation, the combination treatment had a significant regulatory effect on the intestinal microbiota, as evidenced by increases in Lactobacillus spp. and a decrease in Enterobacteriaceae. In addition, the combination treatment increased the concentrations of serum IFN-γ and immunoglobulin G. In conclusion, FOS can be utilized well by L. plantarum ZLP001 and can be combined with it as a potential synbiotic that shows synergistic effects in weaning piglets.

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Use of Lactobacillus plantarum (strains 22F and 25F) and Pediococcus acidilactici (strain 72N) as replacements for antibiotic-growth promotants in pigs

10.21203/rs.3.rs-307288/v1 ◽

2021 ◽

Author(s):

Pawiya Pupa ◽

Prasert Apiwatsiri ◽

Wandee Sirichokchatchawan ◽

Noppadon Pirarat ◽

Tanawong Maison ◽

...

Keyword(s):

Lactobacillus Plantarum ◽

Average Daily Gain ◽

Intestinal Morphology ◽

Pediococcus Acidilactici ◽

Feed Conversion ◽

Peptone Water ◽

Antibiotic Group ◽

Growth Promotants ◽

Spray Dried

Abstract Lactobacillus plantarum (strains 22F and 25F) and Pediococcus acidilactici (strain 72N) have appeared promising in in vitro studies. Microencapsulation, especially by the spray-drying method, has been used to preserve their numbers and characteristics during storage and digestion. This study compared the efficacy of these LAB strains and their microencapsulated form with antibiotic usage on growth performance, faecal microbial counts, and intestinal morphology in nursing-finishing pigs. A total of 240 healthy neonatal pigs were treated on days 0, 3, 6, 9, and 12 after cross-fostering. Sterile peptone water was delivered orally to the control and antibiotic groups. Six-month storage spray-dried Lactobacillus plantarum strain 22F was administered to piglets in the spraydry group. The three fresh strains of LAB (109 CFU/mL) were orally administered to piglets in each group. All pigs received the basal diets, but these were supplemented with routine antibiotic for the antibiotic group. Pigs in the probiotic supplemented groups (spray-dried and three fresh strains of LAB) exhibited a better average daily gain and feed conversion ratio than those of the controls in the nursery and grower phases. Probiotic supplementation increased viable lactobacilli and decreased enterobacterial counts. Antibiotic additives reduced both enterobacterial and lactobacilli counts. Villous height and villous height:crypt depth ratio were greater in probiotic and antibiotic supplemented pigs comparing to the controls, especially in the jejunum. The results demonstrated the feasibility of using these strains as a substitute for antibiotics and the practicality of the microencapsulation protocol for use in swine farms.

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Efeitos da adição de inoculantes microbianos sobre a composição bromatológica e perfil fermentativo da silagem de sorgo produzida em silos experimentais

Revista Brasileira de Zootecnia ◽

10.1590/s1516-35982002000900028 ◽

2002 ◽

Vol 31 (6) ◽

pp. 2373-2379 ◽

Cited By ~ 9

Author(s):

Paulo Henrique Mazza Rodrigues ◽

Ana Lúcia Senatore ◽

Stefano Juliano Tavares de Andrade ◽

Juliana Martins Ruzante ◽

Carlos de Sousa Lucci ◽

...

Keyword(s):

Lactobacillus Plantarum ◽

Pediococcus Acidilactici ◽

Streptococcus Faecium

Foram estudados os efeitos de três inoculantes microbianos, na silagem de sorgo (Agroceres 2005), em 16 silos experimentais, confeccionados a partir de baldes plásticos portando válvulas. O sorgo, cortado aos 97 dias de crescimento (35,9% de MS), foi homogeneizado e submetido a quatro tratamentos, com quatro repetições: controle, Sil-All (Streptococcus faecium, Pediococcus acidilactici, Lactobacillus plantarum, amilase, hemicelulase e celulase), Silobac (L. plantarum, S. faecium e Lactobacillus sp) e Pioneer 1174 (S. faecium e L. plantarum). Os silos foram abertos após 120 dias para análise da composição bromatológica e perfil fermentativo. Os três inoculantes diminuíram o teor de MS e aumentaram a concentração de etanol e as perdas de MS, em relação ao grupo controle. O Sil-All aumentou o teor de FDN, em relação ao grupo controle, e diminuiu a concentração de amido, em relação ao Silobac, enquanto que o Pioneer 1174 diminuiu a estabilidade aeróbia, em relação ao grupo controle. Não foram observados efeitos sobre os teores de PB, NIDA, carboidratos solúveis, FDA, lignina, hemicelulose, celulose, a digestibilidade in vitro da matéria seca, o poder tampão e as concentrações dos ácidos acético, propiônico, butírico e lático, bem como sobre o pH ou sobre as concentrações de nitrogênio amoniacal.

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Lactobacillus plantarum immobilized onto soymilk residue (Okara) for the enhancement of soymilk fermentation and cell survival under simulated gastrointestinal conditions

10.7287/peerj.preprints.2349v1 ◽

2016 ◽

Author(s):

Xia Xiudong ◽

Wang Ying ◽

Liu Xiaoli ◽

Li Ying ◽

Zhou Jianzhong

Keyword(s):

Lactobacillus Plantarum ◽

Specific Growth ◽

Cell Immobilization ◽

Low Ph ◽

Lag Phase ◽

Cell Resistance ◽

Food Grade ◽

Titrable Acidity ◽

Gastrointestinal Conditions

Cell immobilization is an alternative to microencapsulation for the maintenance of cells in a liquid medium. However, artificial immobilization carriers are expensive and pose a high safety risk. This study aimed to evaluate the potential of okara, a food-grade byproduct from soymilk production, as a natural immobilizer for L. plantarum 70810 cells. The study also aimed to evaluate the effects of okara-immobilized L. plantarum 70810 cells on soymilk fermentation, glucosidic isoflavone bioconversion, and cell resistance to gastrointestinal (GI) stress. Scanning electron microscopy revealed that the lactobacilli cells attached and bound to okara’s surface. Compared with the free cells (FL), immobilized Lactobacillus plantarum (IL) cells exhibited a significantly higher specific growth rate and shorter lag phase of growth, a faster decrease in pH and increase in titrable acidity, and a higher soymilk viscosity. Similarly, IL in soymilk showed higher productions of daizein and genistein compared with the control. Compared with FL, IL showed reinforced resistance to simulatedGI stress in vitro that included low pH, low pH plus pepsin, pancreatin, and bile salt. Our results indicate that okara is a new potential immobilization carrier to enhance the growth and glucosidic isoflavone bioconversion activities of L. plantarum in soymilk and improve cell survivability following GI transit.

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Preparation of cultured astrocytes for x-ray microanalysis

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100156924 ◽

1989 ◽

Vol 47 ◽

pp. 988-989

Author(s):

N.K.R. Smith ◽

K.E. Hunter ◽

P. Mobley ◽

L.P. Felpel

Keyword(s):

Cultured Cells ◽

Elemental Content ◽

Elemental Distribution ◽

Sprague Dawley ◽

X Ray ◽

Cultured Astrocytes ◽

Freeze Dried ◽

Ultimate Objective

Electron probe energy dispersive x-ray microanalysis (XRMA) offers a powerful tool for the determination of intracellular elemental content of biological tissue. However, preparation of the tissue specimen , particularly excitable central nervous system (CNS) tissue , for XRMA is rather difficult, as dissection of a sample from the intact organism frequently results in artefacts in elemental distribution. To circumvent the problems inherent in the in vivo preparation, we turned to an in vitro preparation of astrocytes grown in tissue culture. However, preparations of in vitro samples offer a new and unique set of problems. Generally, cultured cells, growing in monolayer, must be harvested by either mechanical or enzymatic procedures, resulting in variable degrees of damage to the cells and compromised intracel1ular elemental distribution. The ultimate objective is to process and analyze unperturbed cells. With the objective of sparing others from some of the same efforts, we are reporting the considerable difficulties we have encountered in attempting to prepare astrocytes for XRMA.Tissue cultures of astrocytes from newborn C57 mice or Sprague Dawley rats were prepared and cultured by standard techniques, usually in T25 flasks, except as noted differently on Cytodex beads or on gelatin. After different preparative procedures, all samples were frozen on brass pins in liquid propane, stored in liquid nitrogen, cryosectioned (0.1 μm), freeze dried, and microanalyzed as previously reported.

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