scholarly journals In vitro immunomodulatory effects of thymol and cinnamaldehyde in a pig intestinal epithelial cell line (IPEC-J2)

2020 ◽  
Vol 8 (3) ◽  
pp. 127-134
Author(s):  
C. Shen ◽  
L.G. Christensen ◽  
P.B. Rasmussen ◽  
K.M. Kragh
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Tight Junctions ◽  
Feed Additives ◽  
Cellular Damage ◽  
Feed Additive ◽  
Epithelial Cell Line ◽  
Rt Pcr ◽  
Wound Recovery

Thymol and cinnamaldehyde are phytogenic feed additives developed to improve gut health and growth performance in poultry and swine. This study evaluated the in vitro immune modulating effects of thymol and cinnamaldehyde blend (TCB) in a porcine gut epithelial cell line (IPEC-J2), with or without cellular damage caused by challenge with lipopolysaccharides. Cytotoxicity, permeability, wound-healing and bacteria adhesion assays were recorded. The expression of cytokines, tight junctions and polymeric immunoglobulin receptor (pIgR) were measured by RT-PCR. The IPEC-J2 cells were cultured in the presence of TCB at concentrations ranging from 1 ng/ml to 1 μg/ml and displayed high viability (>90%). TCB increased barrier integrity (13.8% less in lipopolysaccharide challenge which induced gut epithelial leakage, P<0.05) and accelerated the initial speed of wound recovery (day 1, 26% wound recovery in TCB treated vs 7% in control, P<0.05; day 2, 54 vs 39%, P<0.001). The RT-PCR analysis of cell culture showed that TCB upregulated anti-inflammatory cytokine interleukin (IL)-10 (73.3%, P<0.05) in non-stimulated IPEC-J2 cells, while, when stimulated, pIgR (9.7%, P<0.05) and tight junctions claudin-4 (9.4%, P<0.05) were upregulated by TCB. Furthermore, TCB significantly increased Lactobacillus acidophilus adherence to gut epithelial cells (285.0%, P<0.05). Overall, the current in vitro study showed that TCB can induce various immune responses, which may explain its in vivo benefits as feed additive.

scholarly journals Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line.

1988 ◽  
Vol 106 (3) ◽  
pp. 761-771 ◽  
Author(s):  
P Boukamp ◽  
R T Petrussevska ◽  
D Breitkreutz ◽  
J Hornung ◽  
A Markham ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Human Skin ◽  
Cell Lines ◽  
Hacat Cell ◽  
Epithelial Cell Line ◽  
Spontaneous Transformation ◽  
Chromosomal Alterations ◽  
Hacat Cell Line

In contrast to mouse epidermal cells, human skin keratinocytes are rather resistant to transformation in vitro. Immortalization has been achieved by SV40 but has resulted in cell lines with altered differentiation. We have established a spontaneously transformed human epithelial cell line from adult skin, which maintains full epidermal differentiation capacity. This HaCaT cell line is obviously immortal (greater than 140 passages), has a transformed phenotype in vitro (clonogenic on plastic and in agar) but remains nontumorigenic. Despite the altered and unlimited growth potential, HaCaT cells, similar to normal keratinocytes, reform an orderly structured and differentiated epidermal tissue when transplanted onto nude mice. Differentiation-specific keratins (Nos. 1 and 10) and other markers (involucrin and filaggrin) are expressed and regularly located. Thus, HaCaT is the first permanent epithelial cell line from adult human skin that exhibits normal differentiation and provides a promising tool for studying regulation of keratinization in human cells. On karyotyping this line is aneuploid (initially hypodiploid) with unique stable marker chromosomes indicating monoclonal origin. The identity of the HaCaT line with the tissue of origin was proven by DNA fingerprinting using hypervariable minisatellite probes. This is the first demonstration that the DNA fingerprint pattern is unaffected by long-term cultivation, transformation, and multiple chromosomal alterations, thereby offering a unique possibility for unequivocal identification of human cell lines. The characteristics of the HaCaT cell line clearly document that spontaneous transformation of human adult keratinocytes can occur in vitro and is associated with sequential chromosomal alterations, though not obligatorily linked to major defects in differentiation.

scholarly journals In-vitro cytotoxicity of Trigona itama honey against human lung adenocarcinoma epithelial cell line (A549)

2019 ◽  
Vol 30 ◽  
pp. 100955 ◽  
Author(s):  
Siti Norfitrah Mohd Salim ◽  
Logaraj Ramakreshnan ◽  
Chng Saun Fong ◽  
Ridhwan Abdul Wahab ◽  
Mohammad Syaiful Bahari Abdull Rasad
Keyword(s):  
Epithelial Cell ◽  
Lung Adenocarcinoma ◽  
Cell Line ◽  
Human Lung ◽  
In Vitro Cytotoxicity ◽  
Epithelial Cell Line ◽  
Human Lung Adenocarcinoma

scholarly journals Listeria monocytogenes Infection of Bat Pipistrellus nathusii Epithelial cells Depends on the Invasion Factors InlA and InlB

Pathogens ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 867
Author(s):  
Olga Povolyaeva ◽  
Yaroslava Chalenko ◽  
Egor Kalinin ◽  
Olga Kolbasova ◽  
Elena Pivova ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Epithelial Cells ◽  
Cell Line ◽  
Domestic Animals ◽  
Epithelial Cell Line ◽  
Intracellular Pathogen ◽  
Wild Type ◽  
Listeria Monocytogenes Infection ◽  
Natural Hosts

L. monocytogenes is a widespread facultative intracellular pathogen. The range of natural hosts that supporting L. monocytogenes persistence in the environment has not been fully established yet. In this study, we were interested in the potential of L. monocytogenes to infect cells of bats, which are being increasingly recognized as a reservoir for microorganisms that are pathogenic to humans and domestic animals. A stable epithelial cell line was developed from the kidneys of Pipistrellus nathusii, a small bat widely distributed across Europe. The wild-type L. monocytogenes strain EGDe infected this cell line with an invasion efficiency of 0.0078 ± 0.0009%. Once it entered bat cells, L. monocytogenes doubled within about 70 min. When L. monocytogenes lacked either of the major invasion factors, InlA and InlB, invasion efficiency decreased by a factor of 10 and 25 respectively (p < 0.000001). The obtained results suggest that bat epithelial cells are susceptible to L. monocytogenes infection and that L. monocytogenes invasion of bat cells depends on the major invasion factors InlA and InlB. These results constitute the first report on in vitro studies of L. monocytogenes infection in bats.

Concentration- and time-dependent effects of enoxaparin on human adenocarcinomic epithelial cell line A549 proliferation in vitro

2011 ◽  
Vol 89 (10) ◽  
pp. 705-711 ◽  
Author(s):  
Walid Abu Arab ◽  
Rami Kotb ◽  
Marco Sirois ◽  
Éric Rousseau
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Positive Impact ◽  
A549 Cells ◽  
Cell Counting ◽  
Epithelial Cell Line ◽  
Major Health Problem ◽  
Antineoplastic Effect ◽  
Cell Counts

Non-small cell lung cancer (NSCLC) is a major health problem. Surgery is the only potential curative treatment, in spite of the high recurrence and mortality rates. Low molecular weight heparins (LMWH) have been suggested to have a positive impact on the outcome of various cancers, mainly attributed to their anticoagulant properties; yet a direct antineoplastic effect has not been excluded. We thought to evaluate the direct effect of the LMWH enoxaparin on the human lung adenocarcinomic epithelial cell line A549 and to determine potential antiproliferative and antimetastatic effects that could guide future trials. A549 cells were cultured with different concentrations of enoxaparin (1–30 U/mL). Cell counting was performed at 24, 48, and 72 h. Detection of c-Myc protein and CD44 protein was performed by electrophoresis and Western blotting. Statistical analysis was performed using paired Student’s t tests. Cell counts were decreased with increasing concentrations and time of exposure to enoxaparin. This corresponds to decreased expression of c-Myc and CD44. In conclusion, enoxaparin displayed a direct dose and exposure duration dependent suppressor effect on A549 cell proliferation and the expression of both c-Myc and CD44 in vitro, suggesting reduced proliferative and metastatic potentials of these cells.

scholarly journals Retention of differentiated properties in an established dog kidney epithelial cell line (MDCK).

1979 ◽  
Vol 81 (3) ◽  
pp. 635-648 ◽  
Author(s):  
M J Rindler ◽  
L M Chuman ◽  
L Shaffer ◽  
M H Saier
Keyword(s):  
Epithelial Cell ◽  
Adenylate Cyclase ◽  
Cell Line ◽  
Tight Junctions ◽  
Plasma Membranes ◽  
Mdck Cells ◽  
Morphological Properties ◽  
Epithelial Cell Line ◽  
Kidney Epithelial Cell ◽  
Epithelial Sheets

Madin-Darby canine kidney (MDCK) cells grown in tissue culture have the morphological properties of distal tubular epithelial cells, form tight junctions, and lack several proximal tubular enzyme markers. Adenylate cyclase in these cells was stimulated by vasopressin, oxytocin, prostaglandins E1 and E2, glucagon, and cholera toxin. Hormone-stimulated adenylate cyclase activity in isolated membrane preparations was dependent on low concentrations of GTP and had the MgCl2 and pH optima expected for the kidney enzyme. The results, as well as the demonstration of enhanced hemicyst formation induced by cyclic AMP, suggest that the MDCK cell line has retained the differentiated properties of the kidney epithelial cell of origin. When MDCK cells were injected into baby nude mice, continuous nodule growth was observed until adulthood was attained. Histological studies revealed the presence of two cell types: normal mouse fibroblasts which comprise 80--90% of the solid nodule mass, and MDCK cells, which formed epithelial sheets lining internal fluid-filled glands. Electron microscope analysis showed that the mucosal surfaces of the cells were characterized by microvilli which faced the lumen of the glands, that adjacent MDCK cells were joined by tight junctions, and that the serosal surfaces of the epithelial sheets were characterized by smooth plasma membranes which were lined by a continuous basement membrane. These observations lead to the conclusion that the MDCK cells retain regional differentiation of their plasma membranes and the ability to regenerate kidney tubule-like structures in vivo.

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