Impact of Natural Oil Blend Formulation (NOBF) on Calves during Transportation

Background and Aim: African swine fever is one of the severe pathogens of swine. It has a significant impact on production and economics. So far, there are no known remedies, such as vaccines or drugs, reported working successfully. In the present study, the natural oil blend formulation's (NOBF) efficacy was evaluated against ASFV in vitro using porcine alveolar macrophages (PAMs) cells of swine. Materials and Methods: The capacity of NOBF against the ASFV was tested in vitro. The NOBF combines Eucalyptus globulus, Pinus sylvestris, and Lavandula latifolia. We used a 2-fold serial dilution to test the NOBF formulation dose, that is, 105 HAD50/mL, against purified lethal dose of African swine in primary PAMs cells of swine. The PAM cells survival, real-time polymerase chain reaction (PCR) test, and hemadsorption (HAD) observation were performed to check the NOBF efficacy against ASFV. Results: The in vitro trial results demonstrated that NOBF up to dilution 13 or 0.000625 mL deactivates the lethal dose 105 HAD50 of ASFV. There was no HAD (Rosetta formation) up to dilution 12 or 0.00125 mL of NOBF. The Ct value obtained by running real-time PCR of the NOBF group at 96 h post-infection was the same as the initial value or lower (25), whereas the Ct value of positive controls increased several folds (17.84). Conclusion: The in vitro trial demonstrated that NOBF could deactivate the ASFV. The NOBF has the potential to act as anti-ASFV agent in the field. The next step is to conduct in vivo level trial to determine its efficacy.

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Deactivation of Avian Infectious Bronchitis Virus H120 Using Natural Oil Blend on in vitro Medium

Asian Journal of Poultry Science ◽

10.3923/ajpsaj.2021.24.30 ◽

2020 ◽

Vol 15 (1) ◽

pp. 24-30

Author(s):

Keri Lestari ◽

Haig Babikian ◽

Yusef Babikyan ◽

Imam Megantara ◽

Rajeev Kumar Jha ◽

...

Keyword(s):

Infectious Bronchitis Virus ◽

Avian Infectious Bronchitis Virus ◽

Infectious Bronchitis ◽

Natural Oil ◽

Oil Blend

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Development of a Natural Oil Blend Formulation to Replace Antibiotics and Growth Promoters in Broiler Chicken Feed

International Journal of Poultry Science ◽

10.3923/ijps.2021.199.208 ◽

2021 ◽

Vol 20 (5) ◽

pp. 199-208

Author(s):

Agus Setiyono ◽

Andriyanto . ◽

Aulia Andi Mustika ◽

Lina Noviyanti Sutardi ◽

Haig Babikian ◽

...

Keyword(s):

Broiler Chicken ◽

Growth Promoters ◽

Natural Oil ◽

Oil Blend

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Anti-oxidant and Anti-free Radical Activities of Natural Oil Blend Formulation: Implication for Immune Regulation and Potential Adjuvant Treatment in Infectious Diseases

American Journal of Biomedical Science & Research ◽

10.34297/ajbsr.2020.10.001491 ◽

2020 ◽

Vol 10 (2) ◽

pp. 152-158

Author(s):

Haig Babikian

Keyword(s):

Free Radical ◽

Infectious Diseases ◽

Adjuvant Treatment ◽

Immune Regulation ◽

Natural Oil ◽

Anti Oxidant ◽

Oil Blend

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Influence of Natural Oil Polyol on Mechanical Properties of Polylactic Acid

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.866.208 ◽

2017 ◽

Vol 866 ◽

pp. 208-211 ◽

Cited By ~ 1

Author(s):

Kantima Chaochanchaikul

Keyword(s):

Mechanical Properties ◽

Differential Scanning Calorimetry ◽

Tensile Strength ◽

Polylactic Acid ◽

Castor Oil ◽

Oil Content ◽

Scanning Calorimetry ◽

Elongation At Break ◽

Natural Oil ◽

Oil Blend

The aim of this work was to improve the mechanical properties of polylactic acid (PLA) by natural oil polyol. Castor oil is natural oil polyol used for this work. It was directly extracted from castor seed and without chemical modification. The contents of castor oil were varied from 0 to 10 wt%. The effect of castor oil content on mechanical properties of PLA were evaluated by tensile and impact testings. Differential scanning calorimetry (DSC) and morphology analysis were used for explanation of the result. The result showed that the elongation at break and impact strength of PLA /10 wt% castor oil blend were increased about 108 and 30 % as comparing neat PLA whereas tensile strength tended to decrease about 24 %. The changes in glass transition temperature, crystallinity content and morphology of PLA corresponded well with the result of mechanical properties.

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Natural Oil Blend Formulation (NOBF) as an anti- African Swine Fever Virus (ASFV) agent in Primary Porcine Alveolar Macrophases (PAM) cells of Swine

10.1101/2020.10.09.332890 ◽

2020 ◽

Author(s):

Haig Yousef Babikian ◽

Rajeev Kumar Jha ◽

Quang Lam Truong ◽

Lan Thi Nguyen ◽

Hoa Thi Nguyen ◽

...

Keyword(s):

Pinus Sylvestris ◽

Eucalyptus Globulus ◽

African Swine Fever Virus ◽

African Swine Fever ◽

Fever Virus ◽

Ct Value ◽

Natural Oil ◽

Oil Blend ◽

Lavandula Latifolia

ABSTRACTAfrican swine fever is one of the severe pathogens of swine. It has a significant impact on production and on economics. So far, there are no known remedies, such as vaccines or drugs, reported. The natural oil blend formulation (NOBF) successfully tested against the African swine fever virus (ASFV) in in vitro conditions. The natural oil blend formulation (NOBF) combines Eucalyptus globulus, Pinus sylvestris, and Lavandula latifolia. We used a two-fold serial dilution to test the NOBF formulation dose. The in vitro trial results demonstrated that NOBF up to dilution 13 or 0.000625 ml deactivates the lethal dose 105HAD50 of ASFV. There was no hemadsorption (Rosetta formation) up to dilution 12 or 0.00125 ml of NOBF. The Ct value of the NOBF group at 96 hours post-infection was the same as the initial value or lower (25), whereas the Ct value of positive controls increased several folds (17.84). The in vitro trial demonstrated that NOBF can deactivate the African swine fever virus.HIGHLIGHTSThe natural oil blend formulation (NOBF) was formulated using three natural oils, i.e., Eucalyptus globulus, Pinus sylvestris, and Lavandula latifolia.The in vitro trial was conducted using porcine alveolar macrophages (PAMs) and further passaged in the PAMs; the stock used in the present study was that obtained after the 15th passage.The natural oil blend formulation (NOBF) showed protection against ASF virus up to a dilution of 13 or 0.000625 of a dilution of 16 or 0.000078 ml that was tried.The real-time PCR analysis showed that the virus did not replicate in the NOBF group, which implies that either ASFV growth was inhibited in the presence of NOBF or that it was inactivated.The in vitro trial outcome showed that NOBF has anti-ASFV properties.

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