Effects of Adding Methionine in Low-Protein Diet on Production Performance, Reproductive Organs and Chemical Liver Composition of Laying Hens under Tropical Conditions

2005 ◽  
Vol 4 (5) ◽  
pp. 301-308 ◽  
Author(s):  
Chaiyapoom Bunchasak . ◽  
Taweesak Silapasorn .
2021 ◽  
Vol 8 ◽  
Author(s):  
Miaolin Ma ◽  
Shunju Geng ◽  
Meiling Liu ◽  
Lihong Zhao ◽  
Jianyun Zhang ◽  
...  

This study investigated the effects of different levels of methionine (Met) in a low protein diet on the production performance, reproductive system, metabolism, and gut microbial composition of laying hens to reveal the underlying molecular mechanism of Met in a low protein diet on the host metabolism and gut microbial composition and function of hens. A total of 360 healthy 38-week-old Peking Pink laying hens with similar body conditions and egg production (EP) were randomly divided into four groups with nine replicates per treatment and 10 hens per replicate. The hens in each treatment group were fed low protein diets containing different levels of Met (0.25, 0.31, 0.38, and 0.47%, respectively) for 12 weeks. Feed and water were provided ad libitum throughout the trial period. The results showed that, compared with the 0.25% Met group, the final body weight (FBW), average daily gain (ADG), EP, egg weight (EW), and average daily feed intake (ADFI) in the other groups were significantly increased and feed egg ratio (FER) was decreased. Meanwhile, the EW and yield of abdominal fat (AFY) in the 0.47% Met group were higher than those in other groups. The triglyceride (TG), estradiol (E2), total protein (TP), albumin (ALB), and immunoglobulin A (IgA) in the 0.38 and 0.47% Met groups were higher than those in other groups. In addition, 16S rRNA gene sequencing revealed that there was no difference in the Sobs index, ACE index, and Shannon index among all groups. However, it is worth noting that feeding low protein diets with Met changed the gut microbial composition (e.g., the supplementation of Met increased the level of Lactobacillus and decreased the proportion of Faecalibacterium). Also, our results showed that the changes in gut microbial composition induced by the diets with different levels of Met were closely related to the changes of key parameters: ADFI, EW, FBW, TG, EM, EP, ADG, FER, and uric acid (UA). Our results highlight the role of adding an appropriate amount of Met to the low protein diet in laying hens, which could improve the gut microbial composition, production performance, reproductive system, and nutrient metabolism of laying hens. In conclusion, this study suggested that when the Met level was 0.38%, the production performance of the laying hens was pretty good.


2020 ◽  
Vol 19 (1) ◽  
pp. 51-64
Author(s):  
Chaiwat Arjin ◽  
◽  
Chanmany Souphannavong ◽  
Apinya Sartsook ◽  
Mintra Seel-audom ◽  
...  

Banana stem is a common feed component for raising pigs in mountainous Southeast Asia. However, its nutritive value and digestibility are low. This study was carried out to investigate the effects of unfermented and fermented banana stems on crossbred pigs concerning nutrient digestibility, productive performance, and intestinal morphology. Initially, an in vitro ileal digestibility test was performed for the following feedstuffs: fresh banana stem (BS), fermented banana stem (FBS), concentrate (C), fresh banana stem + concentrate (BSC), and fermented banana stem + concentrate (FBSC). For the 120-day experiment, 16 crossbred pigs were divided into two groups and fed with BSC and FBSC. They were placed in individual cages and subsequently moved to metabolic cages for seven days to determine apparent total tract digestibility (ATTD). Finally, all pigs were slaughtered and their small intestines were analyzed for intestinal morphology. The results show that pure fresh and fermented banana stems had low digestibility. However, their digestibility increased by 50% when mixed with concentrate. Crossbred pigs fed BSC and FBSC did not exhibit significant differences in their performance, but the intestinal morphology of the FBSC group had improved intestinal morphology, especially the villi height. In conclusion, both fresh and fermented banana stems can be recommended in a low protein diet as feed for crossbred pigs in an improved production system. This is relevant for raising pigs in mountainous areas, as it has the potential to reduce feed cost and maintain production performance.


2012 ◽  
Vol 23 (1) ◽  
pp. 151-155 ◽  
Author(s):  
Hai-hua Zhang ◽  
Guang-yu Li ◽  
Xiu-mei Xing ◽  
Er-jun Ren ◽  
Ying Yang ◽  
...  

Author(s):  
F. G. Zaki

Addition of lithocholic acid (LCA), a naturally occurring bile acid in mammals, to a low protein diet fed to rats induced marked inflammatory reaction in the hepatic cells followed by hydropic degeneration and ductular cell proliferation. These changes were accompanied by dilatation and hyperplasia of the common bile duct and formation of “gallstones”. All these changes were reversible when LCA was withdrawn from the low protein diet except for the hardened gallstones which persisted.Electron microscopic studies revealed marked alterations in the hepatic cells. Early changes included disorganization, fragmentation of the rough endoplasmic reticulum and detachment of its ribosomes. Free ribosomes, either singly or arranged in small clusters were frequently seen in most of the hepatic cells. Vesiculation of the smooth endoplasmic reticulum was often encountered as early as one week after the administration of LCA (Fig. 1).


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